Base de dados : LILACS
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Texto completo SciELO Chile
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Id: lil-640532
Autor: Hoenemann, Claudia; Ambold, Juliane; Hohe, Annette.
Título: Gene expression of a putative glutathione S-transferase is responsive to abiotic stress in embryogenic cell cultures of Cyclamen persicum
Fonte: Electron. j. biotechnol;15(1):6-6, Jan. 2012. ilus, tab.
Idioma: en.
Projeto: DFG.
Resumo: Background: Cyclamen persicum is an economically important ornamental crop that is propagated exclusively through seeds as vegetative propagation using cuttings is not possible. Therefore a micropropagation method through somatic embryogenesis is of high interest; however the method suffers from low reliability concerning quality and quantity of the produced plantlets. A crucial step of the protocol is the removal of plant growth regulators (PGRs) that triggers embryo development. In order to get a better insight in this crucial step of the propagation process, a gene expression analysis has been set up using five different genes of glutathione S-transferases (GST) as these are known to be auxin responsive as well as stress reactive. Results: One out of the five genes of glutathione S-transferases (CpGST1) displayed a clear down-regulation 72 hrs after removal of PGRs compared to 4 hrs after, implying auxin responsiveness. However, a more detailed analysis including the time points 0, 4 and 72 hrs revealed an initial strong up-regulation after 4 hrs before it was down-regulated after 72 hrs. In comparison fold-changes of the additional four GST-genes were marginal. Comparing cultures on semisolid medium to that in suspension, transcript abundances of CpGST1 were clearly decreased in suspension culture. Conclusions: Against the initial hypothesis CpGST was not auxin responsive but stress reactive, probably especially indicating drought stress imposed on the cells upon transfer from submerged suspension culture to semisolid medium. Mechanical stress caused by shaking of suspensions cultures seemed to be less important.
Descritores: Cyclamen/enzimologia
Expressão Gênica
Glutationa Transferase/genética
-Técnicas de Cultura de Células
Reação em Cadeia da Polimerase
Estresse Fisiológico
Fatores de Tempo
Responsável: CL1.1 - Biblioteca Central

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Texto completo SciELO Chile
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Id: lil-591930
Autor: Hoenemann, Claudia; Hohe, Annette.
Título: Selection of reference genes for normalization of quantitative real-time PCR in cell cultures of Cyclamen persicum
Fonte: Electron. j. biotechnol;14(1):12-13, Jan. 2011. ilus, tab.
Idioma: en.
Projeto: DFG.
Resumo: As a prerequisite for gene expression analyses in cell cultures of the ornamental crop Cyclamen persicum basic parameters for quantitative real-time polymerase chain reaction (qRT-PCR) have been established including the selection of reference genes using the software tools 'geNorm' and 'NormFinder'. Five potential reference genes have been tested (elongation factor tu (Ef-Tu), putative ABC transporter ATPase, putative conserved oligomeric Golgi (COG) complex component, V-ATPase G subunit 1 and Histone H3-K9 methyltransferase 4 (H3-K9-HMTase 4)). 'NormFinder' as well as 'geNorm' identified Ef-Tu to be the least stable reference gene while the ranking of the most stable genes differed depending on the algorithm. According to 'NormFinder' COG complex component displayed the most stable expression whereas 'geNorm' indicated V-ATPase G subunit 1 and a putative ABC transporter ATPase to be the most reliable reference genes. Hence, we concluded to use a normalization factor calculated from the four reference genes V-ATPase G subunit 1, ABC transporter ATPase, Histone H3-K9 methyltransferase 4 (H3-K9-HMTase 4) and COG complex component for normalization of qRT-PCR in cell cultures of Cyclamen persicum.
Descritores: Cyclamen
Desenvolvimento Embrionário
Desenvolvimento Embrionário/genética
Reação em Cadeia da Polimerase/métodos
Tipo de Publ: Relatório Técnico
Responsável: CL1.1 - Biblioteca Central

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Texto completo SciELO Chile
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Id: lil-558553
Autor: Seyring, Martina; Ewald, Aloma; Mueller, Anke; Haensch, Klaus-Thomas.
Título: Screening for propagation suitability in vitro of different Cyclamen species / Revisión para la adecuación de propagación in vitro de diferentes especies de Cyclamen
Fonte: Electron. j. biotechnol;12(4):10-11, Oct. 2009. ilus, tab.
Idioma: en.
Resumo: In the present study we examined the possibility of propagating different Cyclamen species (C. africanum Boiss. and Reut., C. cilicium Boiss. and Heldr., C. coum Mill., C. hederifolium Ait., C. persicum Mill., C. purpurascens Mill.) including some of their subspecies and cultivars in vitro using explants of adult plants. For this purpose two protocols have been applied to eleven genotypes combined with mostly four explant types (placentas with ovules, leaves, petioles and peduncles). The use of these protocols has given rise to either somatic embryo-like structures and/or adventitious shoots in all genotypes. This way it was possible to propagate each of the examined genotypes in vitro using explants of adult plants in a time less than one year. These results may be used in breeding and propagation of Cyclamen as an ornamental plant and as a medicinal plant.
Descritores: Brotos de Planta/crescimento & desenvolvimento
Brotos de Planta/genética
Cyclamen/crescimento & desenvolvimento
Desenvolvimento Embrionário
Responsável: CL1.1 - Biblioteca Central

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Id: lil-501156
Autor: Brunini, Carlos R. D; Viriato, Ezequiel Paulo.
Título: A Bíblia e a Homeopatia / The Bible and the Homeopathy.
Fonte: São Paulo; Robe; 2003. 224 p.
Idioma: pt.
Descritores: Bíblia
-Arsenicum Album
Arsenicum Metallicum
Atropa belladonna
Bufo rana
Cura pela Fé
Ferrum Metallicum
Hepar Sulphuris Calcareum
Qualidade de Vida
Energia Vital em Homeopatia
Responsável: BR926.1 - Biblioteca Artur de Almeida Rezende Filho
BR926.1; WB930, B896bh, 2003

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