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Id: biblio-967330
Autor: Liao, Yi; Liu, Miao Miao; Li, Qing Ling; Chu, Jun; Su, Jing Jing; Wu, Jia Wen.
Título: Cloning, expression, and characterization of three ribosomal protein S13 genes in Sophora flavescens / Clonagem, expressão e caracterização de três proteínas ribossômicas S13 genes em Sophora flavescens
Fonte: Biosci. j. (Online);34(5):1379-1391, sept./oct. 2018.
Idioma: en.
Resumo: To characterize the structure and function of ribosomal protein S13 (RPS13), we identified fulllength open reading frames (ORFs) of three RPS13 genes (RPS13-1, RPS13-2, and RPS13-3) of the Chinese medicinal plant, Sophora flavescens. The target genes were amplified by reverse transcription-olymerase chain reaction (RT-PCR), ligated into the pET22b(+) vector, and then transformed into Escherichia coli BL21 competent cells for protein expression. The physicochemical properties, protein motif, evolution, and structural organization of the three RPS13 genes were analyzed using bioinformatics tools. The full-length ORFs (453 bp) of the three RPS13 genes of S. flavescens were cloned, and each encodes a protein of 151 amino acids in length, and their expression was detected by Western blotting. Bioinformatics analysis showed that RPS13s are stable proteins that are closely related to the 40S RPS13s of Vigna radiate var. radiate. Their three-dimensional structures included three -helices at the C-terminal and four -helices at the N-terminal, and the two clusters of helices were connected by a long random coil, which may help maintain the dynamic bridging interactions between the large and small subunits of the ribosome. The full-length ORFs of three RPS13 genes of S. flavescens were successfully cloned and expressed in vitro. The study of the physicochemical properties, evolution, and secondary and three-dimensional structures of the three proteins will provide the theoretical basis for further studies on the function of RPS13s in plants.

Objetivo: Para caracterizar a estrutura e a função da proteína ribossomal S13 (RPS13), identificamos fases de leitura abertas (ORFs) completas de três genes RPS13 (RPS13-1, RPS13-2 e RPS13-3) da planta medicinal chinesa, Sophora flavescens. Métodos: Os genes alvo foram amplificados por reação em cadeia da polimerase por transcrição reversa (RT-PCR), ligados ao vetor pET22b(+), e então transformados em células competentes de Escherichia coli BL21 para expressão protéica. As propriedades físico-químicas, o motivo protéico, a evolução e a organização estrutural dos três genes RPS13 foram analisados utilizando ferramentas de bioinformática. Resultados: ORFs completos (453 pb) dos três genes RPS13 de S. flavescens foram clonados, e cada um codifica uma proteína de 151 aminoácidos de comprimento, e sua expressão foi detectada por western blotting. A análise de bioinformática mostrou que as RPS13s são proteínas estáveis que estão intimamente relacionadas com as 40S RPS13s de Vigna radiata var. radiate. Suas estruturas tridimensionais incluíam três -hélices no C-terminal e quatro -hélices no N-terminal, e os dois aglomerados de hélices eram conectados por uma longa bobina aleatória, o que pode ajudar a manter as interações de ponte dinâmicas entre o subunidades grandes e pequenas do ribossomo. Conclusões: As ORFs completas de três genes RPS13 de S. flavescens foram clonadas e expressas com sucesso in vitro. O estudo das propriedades físico-químicas, evolução e estruturas secundárias e tridimensionais das três proteínas fornecerão a base teórica para estudos adicionais sobre a função das RPS13s em plantas.
Descritores: Biologia Computacional
Sophora
Transcrição Reversa
Escherichia coli
Genes
Responsável: BR396.1 - Biblioteca Central


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Texto completo SciELO Brasil
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Id: biblio-889171
Autor: Toma, Maíra Akemi; Carvalho, Teotonio Soares de; Guimarães, Amanda Azarias; Costa, Elaine Martins da; Silva, Jacqueline Savana da; Moreira, Fatima Maria de Souza.
Título: Tripartite symbiosis of Sophora tomentosa, rhizobia and arbuscular mycorhizal fungi
Fonte: Braz. j. microbiol;48(4):680-688, Oct.-Dec. 2017. tab, graf.
Idioma: en.
Resumo: ABSTRACT Sophora tomentosa is a pantropical legume species with potential for recovery of areas degraded by salinization, and for stabilization of sand dunes. However, few studies on this species have been carried out, and none regarding its symbiotic relationship with beneficial soil microorganisms. Therefore, this study aimed to evaluate the diversity of nitrogen-fixing bacteria isolated from nodules of Sophora tomentosa, and to analyze the occurrence of colonization of arbuscular mycorrhizal fungi on the roots of this legume in seafront soil. Thus, seeds, root nodules, and soil from the rhizosphere of Sophora tomentosa were collected. From the soil samples, trap cultures with this species were established to extract spores and to evaluate arbuscular mycorhizal fungi colonization in legume roots, as well as to capture rhizobia. Rhizobia strains were isolated from nodules collected in the field or from the trap cultures. Representative isolates of the groups obtained in the similarity dendrogram, based on phenotypic characteristics, had their 16S rRNA genes sequenced. The legume species showed nodules with indeterminate growth, and reddish color, distributed throughout the root. Fifty-one strains of these nodules were isolated, of which 21 were classified in the genus Bacillus, Brevibacillus, Paenibacillus, Rhizobium and especially Sinorhizobium. Strains closely related to Sinorhizobium adhaerens were the predominant bacteria in nodules. The other genera found, with the exception of Rhizobium, are probably endophytic bacteria in the nodules. Arbuscular mycorrhizal fungi was observed colonizing the roots, but arbuscular mycorhizal fungi spores were not found in the trap cultures. Therefore Sophora tomentosa is associated with both arbuscular mycorhizal fungi and nodulating nitrogen-fixing bacteria.
Descritores: Bactérias/isolamento & purificação
Fungos/isolamento & purificação
Micorrizas/isolamento & purificação
Sophora/microbiologia
Simbiose
-Fenômenos Fisiológicos Bacterianos
Bactérias/classificação
Bactérias/genética
Fungos/classificação
Fungos/genética
Fungos/fisiologia
Micorrizas/classificação
Micorrizas/genética
Micorrizas/fisiologia
Filogenia
Raízes de Plantas/microbiologia
Microbiologia do Solo
Sophora/fisiologia
Responsável: BR1.1 - BIREME


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Texto completo SciELO Costa Rica
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Id: lil-753793
Autor: Luzia Delgado, Carolina Maria; Souza de Paula, Alexandre; Santos, Marisa; Silveira Paulilo, Maria Terezinha.
Título: Dormancy-breaking requirements of Sophora tomentosa and Erythrina speciosa (Fabaceae) seeds / Dormancy-breaking requirements of Sophora tomentosa and Erythrina speciosa (Fabaceae) seeds
Fonte: Rev. biol. trop;63(1):285-294, Jan.-Mar. 2015. ilus, graf, tab.
Idioma: en.
Resumo: The physical dormancy of seeds has been poorly studied in species from tropical forests, such as the Atlantic Forest. This study aimed to examine the effect of moderate alternating temperatures on breaking the physical dormancy of seeds, the morphoanatomy and histochemistry of seed coats, and to locate the structure/region responsible for water entrance into the seed, after breaking the physical dormancy of seeds of two woody Fabaceae (subfamily Faboideae) species that occur in the Brazilian Atlantic Forest: Sophora tomentosa and Erythrina speciosa. To assess temperature effect, seeds were incubated in several temperature values that occur in the Atlantic Forest. For morphological and histochemical studies, sections of fixed seeds were subjected to different reagents, and were observed using light or epifluorescence microscopy, to analyze the anatomy and histochemistry of the seed coat. Treated and non-treated seeds were also analyzed using a scanning electron microscope (SEM) to observe the morphology of the seed coat. To localize the specific site of water entrance, the seeds were blocked with glue in different regions and also immersed in ink. In the present work a maximum temperature fluctuation of 15ºC was applied during a period of 20 days and these conditions did not increase the germination of S. tomentosa or E. speciosa. These results may indicate that these seeds require larger fluctuation of temperature than the applied or/and longer period of exposition to the temperature fluctuation. Blocking experiments water inlet combined with SEM analysis of the structures of seed coat for both species showed that besides the lens, the hilum and micropyle are involved in water absorption in seeds scarified with hot water. In seeds of E. speciosa the immersion of scarified seeds into an aniline aqueous solution showed that the solution first entered the seed through the hilum. Both species showed seed morphological and anatomical features for seed coats of the subfamily Faboideae. Lignin and callose were found around all palisade layers and the water impermeability and ecological role of these substances are discussed in the work.

Requisitos para romper la latencia en semillas de Sophora tomentosa y Erythrina speciosa (Fabaceae). La latencia física de las semillas ha sido poco estudiada en las especies de los bosques tropicales, como el bosque atlántico. Este estudio tuvo como objetivo examinar el efecto de las temperaturas moderadas alternantes en romper la latencia física de las semillas, la anatomía y la histoquímica de la cubierta de las semillas, y la localización de la estructura o región responsable de la entrada de agua, después de romper la latencia física de las semillas de Sophora tomentosa y Eythrina speciosa, dos especies leñosas de Fabaceae (subfamilia Faboideae) que presentes en el bosque atlántico de Brasil. Para cumplir con el primer objetivo se incubaron las semillas a varias temperaturas que se dan en el bosque atlántico. Para los estudios morfológicos e histoquímicos se fijaron secciones de semillas sometidos a diferentes reactivos y se observaron usando luz o microscopía de epifluorescencia para analizar la anatomía y la histoquímica de la cubierta de la semilla. Semillas tratadas y no tratadas se analizaron también usando un microscopio electrónico de barrido (MDB) o microscopio estereoscópico (ME) para observar la morfología de la cubierta de la semilla. Para localizar el sitio específico de la entrada de agua, las semillas fueron bloqueadas con pegamento en diferentes regiones y también sumergidas en tinta. En el presente trabajo se aplicó una fluctuación de temperatura máxima de 15°C durante un período de 20 días y estas condiciones no aumentó la germinación de S. tomentosa o E. speciosa. Estos resultados pueden indicar que estas semillas requieren mayor fluctuación de la temperatura que la aplicada y/o un período más largo de exposición a la fluctuación de la temperatura. Experimentos de bloqueo de entrada de agua combinada con el análisis de las estructuras de la cubierta de la semilla para ambas especies (SEM) mostró que, a pesar de la lente, el hilio y micropilo están implicados en la absorción de agua en las semillas escarificadas con agua caliente. En las semillas de E. speciosa la inmersión de semillas escarificadas en una solución acuosa de anilina mostró que la solución entró por primera vez a la semilla a través del hilio. Ambas especies mostraron características morfológicas y anatómicas de semillas con cubierta de la subfamilia Faboideae. La lignina y callosa se encontraron alrededor de todas las capas de empalizada y la impermeabilidad al agua y en el trabajo se discute el papel ecológico de estas sustancias.
Descritores: Erythrina/crescimento & desenvolvimento
Germinação/fisiologia
Dormência de Plantas/fisiologia
Sementes/crescimento & desenvolvimento
Sophora/crescimento & desenvolvimento
-Microscopia Eletrônica de Varredura
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CR1.1 - BINASSS - Biblioteca Nacional de Salud y Seguridad Social


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Texto completo SciELO Brasil
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Id: lil-688594
Autor: Zhao, Long Fei; Xu, Ya Jun; Ma, Zhan Qiang; Deng, Zhen Shan; Shan, Chang Juan; Wei, Ge Hong.
Título: Colonization and plant growth promoting characterization of endophytic Pseudomonas chlororaphis strain Zong1 isolated from Sophora alopecuroides root nodules
Fonte: Braz. j. microbiol;44(2):629-637, 2013. ilus, tab.
Idioma: en.
Projeto: 973 Project of China; . National Science Foundation of China; . Henan Provincial Department of Science and Technology Research Project; . Henan Provincial Education Department of Science and Technology Research Key Research Project; . Foundation for University Key Teacher by the Ministry of Education of Henan Province.
Resumo: The endophytic strain Zong1 isolated from root nodules of the legume Sophora alopecuroides was characterized by conducting physiological and biochemical tests employing gfp-marking, observing their plant growth promoting characteristics (PGPC) and detecting plant growth parameters of inoculation assays under greenhouse conditions. Results showed that strain Zong1 had an effective growth at 28 ºC after placed at 4-60 ºC for 15 min, had a wide range pH tolerance of 6.0-11.0 and salt tolerance up to 5% of NaCl. Zong1 was resistant to the following antibiotics (µg/mL): Phosphonomycin (100), Penicillin (100) and Ampicillin (100). It could grow in the medium supplemented with 1.2 mmol/L Cu, 0.1% (w/v) methylene blue and 0.1-0.2% (w/v) methyl red, respectively. Zong1 is closely related to Pseudomonas chlororaphis based on analysis the sequence of 16S rRNA gene. Its expression of the gfp gene indicated that strain Zong1 may colonize in root or root nodules and verified by microscopic observation. Furthermore, co-inoculation with Zong1 and SQ1 (Mesorhizobium sp.) showed significant effects compared to single inoculation for the following PGPC parameters: siderophore production, phosphate solubilization, organic acid production, IAA production and antifungal activity in vitro. These results suggest strains P. chlororaphi Zong1 and Mesorhizobium sp. SQ1 have better synergistic or addictive effect. It was noteworthy that each growth index of co-inoculated Zong1+SQ1 in growth assays under greenhouse conditions is higher than those of single inoculation, and showed a significant difference (p < 0.05) when compared to a negative control. Therefore, as an endophyte P. chlororaphis Zong1 may play important roles as a potential plantgrowth promoting agent.
Descritores: Endófitos/isolamento & purificação
Endófitos/metabolismo
Pseudomonas/isolamento & purificação
Pseudomonas/metabolismo
Sophora/microbiologia
-Antibiose
Antibacterianos/farmacologia
Análise por Conglomerados
Ácidos Carboxílicos/metabolismo
DNA Bacteriano/química
DNA Bacteriano/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Endófitos/classificação
Endófitos/genética
Fungos/crescimento & desenvolvimento
Concentração de Íons de Hidrogênio
Ácidos Indolacéticos/metabolismo
Dados de Sequência Molecular
Filogenia
Desenvolvimento Vegetal
Fosfatos/metabolismo
Raízes de Plantas/microbiologia
Pseudomonas/classificação
Pseudomonas/genética
/genética
RNA, RIBOSOMAL, ABNORMALITIES, MULTIPLES/genética
Análise de Sequência de DNA
Sideróforos/metabolismo
Cloreto de Sódio/metabolismo
Sophora/crescimento & desenvolvimento
Temperatura
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-461361
Autor: Ma, X. M; Bao, G. Sh; Wan, J. M; Liao, D. J; Yin, Sh. F; Meng, X. Q; Zhou, G. K; Lu, X. M; Li, H. Y.
Título: Therapeutic effects of Sophora moorcroftiana alkaloids in combination with albendazole in mice experimentally infected with protoscolices of Echinococcus granulosus
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;40(10):1403-1408, Oct. 2007. ilus, tab.
Idioma: en.
Resumo: The objective of the present study was to determine if the combination of alkaloids from Sophora moorcroftiana seeds and albendazole might be effective in the treatment of experimental echinococcosisin female NIH mice (6 weeks old and weighing 18-20 g, N = 8 in each group) infected withprotoscolices of Echinococcus granulosus. Viable protoscolices (N = 6 x 103) were cultured in vitro in 1640 medium and mortality was calculated daily. To determine the in vivo efficacy, mice were inoculated intraperitoneally with viable protoscolices and then treated once daily by gavage for three months with the alkaloids (50 mg kg-1 day-1) and albendazole (50 mg kg-1 day-1), separately and in combination (both alkaloids at 25 mg kg-1 day-1 and albendazole at 25 mg kg-1 day-1). Next, the hydatid cysts collected from the peritoneal cavity of the animals were weighed and serum IL-4, IL-2, and IgE levels were analyzed. Administration of alkaloids to cultured protoscolices showed significant dose- and time-dependent killing effects. The weight of hydatid cysts was significantly decreased upon treatment with each drug (P < 0.01), but the decrease was more prominent and the rate of hydatid cyst growth inhibition was much higher (76.1 percent) in the group receiving the combined treatments (18.3 ± 4.6 mg). IL-4 and total IgE were decreased (939 ± 447 pg/mL and 2.03 ± 0.42 IU/mL, respectively) in serum from mice treated with alkaloids and albendazole compared with the untreated control (1481 ± 619 pg/mL and 3.31 ± 0.37 IU/mL; P < 0.01). These results indicate that S. moorcroftiana alkaloids have protoscolicidal effects and the combination of alkaloids and albendazole has significant additive effects.
Descritores: Albendazol/administração & dosagem
Alcaloides/administração & dosagem
Anticestoides/administração & dosagem
Equinococose/tratamento farmacológico
Echinococcus granulosus/efeitos dos fármacos
Sophora/química
-Modelos Animais de Doenças
Quimioterapia Combinada
Equinococose/imunologia
Equinococose/patologia
Imunoglobulina E/sangue
/sangue
INTERLEUKIN-ABBREVIATIONS AS TOPIC/sangue
/sangue
INTERLEUKIN-TEMEFOS/sangue
Camundongos Endogâmicos
Sementes/química
Fatores de Tempo
Limites: Animais
Feminino
Camundongos
Responsável: BR1.1 - BIREME



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