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Texto completo SciELO Brasil
Texto completo
Id: biblio-951809
Autor: Paz, Jorge Enrique Wong; Contreras, Carolina Rubio; Munguía, Abigail Reyes; Aguilar, Cristóbal Noé; Inungaray, María Luisa Carrillo.
Título: Phenolic content and antibacterial activity of extracts of Hamelia patens obtained by different extraction methods
Fonte: Braz. j. microbiol;49(3):656-661, July-Sept. 2018. tab, graf.
Idioma: en.
Resumo: Abstract Hamelia patens, is a plant traditionally used to treat a variety of conditions among the Huastec people of Mexico. The objective of this study is to characterize the phenolic content and critically examine the antimicrobial activity of leaf extracts H. patens, obtained by maceration, Soxhlet and percolation, using ethanol as 70% solvent. Phenolic compounds are characterized by liquid chromatography, coupled to a High Resolution Mass Spectrometry, and the antimicrobial activity was studied from the inhibitory effect of each extract for Escherichia coli, Staphylococcus aureus, Salmonella typhi and S. paratyphi, and by the Minimum Bactericidal Concentration, the percentage of activity and the Index of Bacterial Susceptibility of each extract. The phenolic compound identified in different concentrations in the three extracts was epicatechin. The extracts obtained by the three methods had antimicrobial activity, however, there was no significant difference (p < 0.05) between the Minimum Bactericidal Concentration of the extracts obtained by maceration, percolation and Soxhlet. The results of this study contribute to the body of knowledge on the use of extracts in controlling microorganisms with natural antimicrobials.
Descritores: Fenóis/isolamento & purificação
Extratos Vegetais/isolamento & purificação
Extratos Vegetais/farmacocinética
Fracionamento Químico/métodos
Antibacterianos/isolamento & purificação
Staphylococcus aureus/efeitos dos fármacos
Extratos Vegetais/química
Testes de Sensibilidade Microbiana
Escherichia coli/efeitos dos fármacos
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME

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