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Pesquisa : B01.650.940.800.575.912.250.583.520.362 [Categoria DeCS]
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Id: biblio-950753
Autor: Zeb, Anwar; Sadiq, Abdul; Ullah, Farhat; Ahmad, Sajjad; Ayaz, Muhammad.
Título: Phytochemical and toxicological investigations of crude methanolic extracts, subsequent fractions and crude saponins of Isodon rugosus
Fonte: Biol. Res;47:1-6, 2014. tab.
Idioma: en.
Resumo: BACKGROUND: Isodon rugosus is used traditionally in the management of hypertension, rheumatism, tooth-ache and pyrexia. Present study was arranged to investigate I. rugosus for phytoconstituents, phytotoxic and cytotoxic activities to explore its toxicological, pharmacological potentials and to rationalize its ethnomedicinal uses. Briefly, qualitative phytochemical analysis of the plant extracts were carried out for the existence of alkaloids, flavonoids, saponins, oils, glycosides, anthraquinones, terpenoids, sterols and tannins. Plant crude methanolic extract (Ir.Cr), its subsequent fractions; n-hexane (Ir.Hex), chloroform (Ir.Chf), ethyl acetate (Ir.EtAc), aqueous (Ir.Aq) and saponins (Ir.Sp) in different concentrations were tested for phytotoxic and cytotoxic activities using radish seeds and brine shrimps (Artemia salina) respectively. The phytotoxic activity was determined by percent root length inhibition (RLI) and percent seeds germination inhibition (SGI) while the cytotoxicity was obtained with percent lethality of the brine shrimps. RESULTS: Ir.Cr was tested positive for the presence of alkaloids, glycosides, flavonoids, oils, terpenoids, saponins, tannins and anthraquinones. Among different fractions Ir.Sp, Ir.Chf, Ir.EtAc, and Ir.Cr were most effective causing 93.55, 89.32, 81.32 and 58.68% inhibition of seeds in phytotoxicity assay, with IC50 values of 0.1, 0.1, 0.1 and 52 µg/ml respectively. Similarly, among all the tested samples, Ir.Sp exhibited the highest phytotoxic effect causing 91.33% root length inhibition with IC50 of 0.1 µg/ml. Ir.Sp and Ir.Chf were most effective against brine shrimps showing 92.23 and 76.67% lethality with LC50 values of 10 and 12 µg/ml respectively. CONCLUSIONS: It may be inferred from the current investigations that I. rugosus contains different secondary metabolites and is a potential source for the isolation of natural anticancer and herbicidal drug molecules. Different fractions exhibited phytotoxic and cytotoxic activities, thus providing pharmacological basis for ethnomedicinal uses of this plant.
Descritores: Saponinas/análise
Extratos Vegetais/farmacologia
Isodon/química
Fitoterapia
-Artemia/efeitos dos fármacos
Taninos/análise
Terpenos/análise
Flavonoides/análise
Óleos Vegetais/análise
Clorofórmio
Antraquinonas/análise
Raízes de Plantas/crescimento & desenvolvimento
Citotoxinas
Componentes Aéreos da Planta/química
Metanol
Alcaloides/análise
Glicosídeos/análise
Hexanos
Limites: Animais
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-950772
Autor: Zeb, Anwar; Sadiq, Abdul; Ullah, Farhat; Ahmad, Sajjad; Ayaz, Muhammad.
Título: Investigations of anticholinestrase and antioxidant potentials of methanolic extract, subsequent fractions, crude saponins and flavonoids isolated from Isodon rugosus
Fonte: Biol. Res;47:1-10, 2014. graf, tab.
Idioma: en.
Resumo: BACKGROUND: Based on the ethnomedicinal uses and the effective outcomes of natural products in various diseases, this study was designed to evaluate Isodon rugosus as possible remedy in oxidative stress, alzheimer's and other neurodegenerative diseases. Acetylecholinestrase (AChE) and butyrylcholinesterase (BChE) inhibitory activities of crude methanolic extract (Ir.Cr), resultant fractions (n-hexane (Ir.Hex), chloroform (Ir.Cf), ethyl acetate (Ir.EtAc), aqueous (Ir.Aq)), flavonoids (Ir.Flv) and crude saponins (Ir.Sp) of I. rugosus were investigated using Ellman's spectrophotometric method. Antioxidant potential of I. rugosus was determined using DPPH, H2O2 and ABTS free radicals scavenging assays. Total phenolic and flavonoids contents of plant extracts were determined and expressed in mg GAE/g dry weight and mg RTE/g of dry sample respectively. RESULTS: Among different fractions Ir.Flv and Ir.Cf exhibited highest inhibitory activity against AChE (87.44 ± 0.51, 83.73 ± 0.64%) and BChE (82.53 ± 0.71, 88.55 ± 0.77%) enzymes at 1 mg/ml with IC50 values of 45, 50 for AChE and 40, 70 µg/ml for BChE respectively. Activity of these fractions were comparable to galanthamine causing 96.00 ± 0.30 and 88.61 ± 0.43% inhibition of AChE and BChE at 1 mg/ml concentration with IC50 values of 20 and 47 µg/ml respectively. In antioxidant assays, Ir.Flv, Ir.Cf, and Ir.EtAc demonstrated highest radicals scavenging activities in DPPH and H2O2 assays which were comparable to ascorbic acid. Ir.Flv was found most potent with IC50 of 19 and 24 µg/ml against DPPH and H2O2 radicals respectively. Whereas antioxidant activates of plant samples against ABTS free radicals was moderate. Ir.Cf, Ir.EtAc and Ir.Cr showed high phenolic and flavonoid contents and concentrations of these compounds in different fractions correlated well to their antioxidant and anticholinestrase activities. CONCLUSION: It may be inferred from the current investigations that the Ir.Sp, Ir.Flv and various fractions of I. rugosus are good sources of anticholinesterase and antioxidant compounds. Different fractions can be subjected to activity guided isolation of bioactive compounds effective in neurological disorders.
Descritores: Saponinas/análise
Flavonoides/análise
Extratos Vegetais/química
Inibidores da Colinesterase/análise
Isodon/química
Antioxidantes/análise
-Picratos/metabolismo
Acetilcolinesterase/efeitos dos fármacos
Saponinas/isolamento & purificação
Espectrofotometria/métodos
Ácidos Sulfônicos/metabolismo
Flavonoides/isolamento & purificação
Compostos de Bifenilo/metabolismo
Butirilcolinesterase/efeitos dos fármacos
Clorofórmio
Sequestradores de Radicais Livres/metabolismo
Estresse Oxidativo/efeitos dos fármacos
Concentração Inibidora 50
Isodon/classificação
Isodon/enzimologia
Componentes Aéreos da Planta/química
Misturas Complexas
Metanol
Benzotiazóis/metabolismo
Radicais Livres/análise
Hexanos
Peróxido de Hidrogênio/metabolismo
Medicina Tradicional
Acetatos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-907515
Autor: Joung Ha, Tae; Isobe, Takahiko; Werner, Enrique; Alarcón, Julio; Kubo, Isao; Cespedes, Carlos L.
Título: Pedalitin from isodon japonica, an inactivation of soybean lipoxygenase-1 / Pedalitina de isodon japonica, un inactivador de la lipo oxigenasa-1 de poroto de soya
Fonte: Bol. latinoam. Caribe plantas med. aromát;15(1):29-40, ene. 2016. ilus, graf, tab.
Idioma: en.
Projeto: CONICYT-Chile.
Resumo: Pedalitin, isolated from the aerial part of Rabdosia japonica (Labiatae), inhibited soybean lipoxygenase-1 (EC 1.13.11.12, Type I) with an IC50 of 152.5 uM. The progress curves for an enzyme reaction, pedalitin inactivate the lipoxygenase-1 in a time dependent, irreversible manner, exhibiting kinetics with a kinact/KI of 59.6 +/- 10 mM-1min-1. In the pseudoperoxidase activity, pedalitin is very slowly oxidized by the soybean lipoxygenase-1 catalyzed decomposition of lipid hydroperoxides.

Pedalitina, aislada de las partes aereas de Rabdosia japonica inhibió a la lipooxigenasa-1 (EC 1.13.11.12 tipo I) con un IC50 de 152.5 uM. La curva de progreso para una acción enzimática, pedalitina inactivó a la lipooxigenasa-1 de una manera dependiente del tiempo, de una manera irreversible, exhibiendo una cinética con una kinact/KI de 59.6 +/- mM-1min-1. En la actividad pseudoperoxidasa, pedalitina es oxidada lentamente por la descomposición de la lípido hidroperóxido de la lipooxigenasa-1 de poroto de soya.
Descritores: Flavonas/isolamento & purificação
Flavonas/farmacologia
Isodon/química
Inibidores de Lipoxigenase/farmacologia
Soja/enzimologia
-Cinética
Inibidores de Lipoxigenase/isolamento & purificação
Fatores de Tempo
Responsável: CL1.1 - Biblioteca Central



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