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Id: biblio-1047976
Autor: Yang, Xiulian; Ding, Wenjie; Yue, Yuanzheng; Xu, Chen; Wang, Xi; Wang, Lianggui.
Título: Cloning and expression analysis of three critical triterpenoid pathway genes in Osmanthus fragrans
Fonte: Electron. j. biotechnol;36:1-8, nov. 2018. ilus, graf.
Idioma: en.
Projeto: Application Demonstration of the Key Technology and Innovation in Breeding the Six Precious Colored Tree Species of Jiangsu Province; . Academic Programs Project of Jiangsu Higher Education Institutions.
Resumo: Background: Osmanthus fragrans is an important ornamental tree and has been widely planted in China because of its pleasant aroma, which is mainly due to terpenes. The monoterpenoid and sesquiterpenoid metabolic pathways of sweet osmanthus have been well studied. However, these studies were mainly focused on volatile small molecule compounds. The molecular regulation mechanism of synthesis of large molecule compounds (triterpenoids) remains unclear. Squalene synthase (SQS), squalene epoxidase (SQE), and beta-amyrin synthase (BETA-AS) are three critical enzymes of the triterpenoid biosynthesis pathway. Results: In this study, the full-length cDNA and gDNA sequences of OfSQS, OfSQE, and OfBETA-AS were isolated from sweet osmanthus. Phylogenetic analysis suggested that OfSQS and OfSQE had the closest relationship with Sesamum indicum, and OfBETA-AS sequence shared the highest similarity of 99% with that of Olea europaea. The qRT-PCR analysis revealed that the three genes were highly expressed in flowers, especially OfSQE and OfBETA-AS, which were predominantly expressed in the flowers of both "Boye" and "Rixiang" cultivars, suggesting that they might play important roles in the accumulation of triterpenoids in flowers of O. fragrans. Furthermore, the expression of OfBETA-AS in the two cultivars was significantly different during all the five flowering stages; this suggested that OfBETA-AS may be the critical gene for the differences in the accumulation of triterpenoids. Conclusion: The evidence indicates that OfBETA-AS could be the key gene in the triterpenoid synthesis pathway, and it could also be used as a critical gene resource in the synthesis of essential oils by using bioengineered bacteria.
Descritores: Triterpenos/metabolismo
Clonagem Molecular
-Farnesil-Difosfato Farnesiltransferase/metabolismo
Óleos Voláteis
Expressão Gênica
Reação em Cadeia da Polimerase
Esqualeno Mono-Oxigenase/metabolismo
Responsável: CL1.1 - Biblioteca Central

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Id: biblio-859948
Autor: Emer, Aquélis Armiliato; Oliveira, Marisa de Cacia; Althaus-Ottmann, Michelle Melissa.
Título: Biochemical composition and germination capacity of Ligustrum lucidum ait. seeds in the process of biological invasion / Composição bioquímica e capacidade de germinação de sementes de Ligustrum lucidum ait. no processo de invasão biológica
Fonte: Acta sci., Biol. sci;34(3):353-357, July-Sept. 2012. ilus.
Idioma: en.
Resumo: Ligustrum lucidum ait. is an exotic species and In the present study, contents of sugars, proteins, and germination were evaluated in seeds removed from not abscisic and abscisic fruits. The seeds were stored or not under shadow or sun. The higher contents of total and reducing sugars were observed in seeds removed from not abscisic fruit while the period of storage resulted in the loss of these compounds. The same result was observed in the content of total sugars of seeds from abscisic fruits. The content of reducing sugars in seeds from abscisic fruits and submitted to storage had its content increased. The contents of total proteins in seeds from abscisic and not abscisic submitted to shadow or sun were not significantly different, but the results suggested an increase in protein synthesis during the storage period, differently from those seeds that were not submitted to storage. When seeds from abscisic and not abscisic fruits were evaluated immediately after harvest, they presented greater percentages of germination, while after 28 days, seeds submitted to the storage treatments showed lower values. The invasive potential of L. lucidum seems to be more associated to the number of seeds produced, than to their germination potential.

Ligustrum lucidum ait. é uma espécie exótica e no presente trabalho foram avaliados os teores de açúcares totais, redutores, proteínas, e germinação de sementes retiradas de frutos coletados da planta-mãe (não abscisados) e dispersos (abscisados) mantidos ou não em condições de sombra ou sol. Os maiores teores de açúcares totais e redutores foram obtidos em sementes imaturas, enquanto o armazenamento levou à perda destes composto, o que foi observado também para açúcares totais, nas mesmas condições, para sementes maduras. Verificaram-se aumentos nos teores de açúcares redutores em sementes maduras armazenadas. Apesar de não serem estatisticamente significativos, estes resultados entre sementes maduras e imaturas submetidas ao mesmo tratamento, indicam possível aumento da síntese protéica durante o período de armazenamento, sugerido pelo acréscimo no teor de proteínas totais, com pequena perda e/ou inibição em sementes de frutos submetidos ao sol. Sementes maduras e imaturas, imediatamente testadas após a coleta, apresentaram os maiores valores de porcentagem de germinação. no final de 28 dias de avaliação, enquanto aquelas submetidas aos tratamentos de armazenamento ficaram muito abaixo destes valores. Pelo presente trabalho, o potencial invasor de L. lucidum parece estar relacionado mais ao número de sementes produzidas pela espécie que o seu potencial germinativo.
Descritores: Carboidratos
Responsável: BR513.2 - Editora da Universidade Estadual de Maringá

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Id: lil-591920
Autor: Yuan, Wang Jun; Han, Yuan Ji; Dong, Mei Fang; Shang, Fu De.
Título: Assessment of genetic diversity and relationships among osmanthus fragrans cultivars using AFLP markers
Fonte: Electron. j. biotechnol;14(1):2-3, Jan. 2011. ilus, tab.
Idioma: en.
Projeto: National Natural Science Funds in China; . Innovation Scientists and Technicians Troop Construction Projects of Henan Province.
Resumo: This study was conducted to reveal genetic diversity among 100 Osmanthus fragrans cultivars using amplified fragment length polymorphism (AFLP) markers. Eight AFLP primer combinations produced a total of 443 polymorphic fragments with an average of 64 per primer combination. The percentage of polymorphic bands (86.81 percent), the resolving power (Rp) (32.71) and the PIC values (0.331) showed the efficiency of used primer combinations. The revealed AFLP makers were effective in distinguishing all the cultivars considered. Cluster analysis were performed to assess patterns of diversity among cultivars and showed the abundant genetic diversity. The overall distribution pattern of molecular variation suggested that 93.33 percent of the total genetic variance was within the identified groups and 6.67 percent of the genetic variation was among the identified groups. Our results showed that AFLP markers are useful for Osmanthus fragrans germplasm discrimination as well as for investigation of genetic diversity and variation. The information will facilitate germplasm identification, conservation and new cultivar development.
Descritores: DNA
-Cultivos Agrícolas
Análise do Polimorfismo de Comprimento de Fragmentos Amplificados
Técnicas de Diagnóstico Molecular/métodos
Variação Genética/genética
Responsável: CL1.1 - Biblioteca Central

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