Base de dados : LILACS
Pesquisa : B01.650.940.800.575.912.250.595.400.149 [Categoria DeCS]
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Id: biblio-1015852
Autor: Yan, Kuan; Wei, Qin; Feng, Ruizhang; Zhou, Wanhai; Chen, Fang.
Título: Transcriptome analysis of Cinnamomum longepaniculatum by high-throughput sequencing
Fonte: Electron. j. biotechnol;28:58-66, July. 2017. tab, graf, ilus.
Idioma: en.
Projeto: Sichuan Education Department; . Innovative Program of Sichuan Undergraduates; . Key Lab of Aromatic Plant Resources Exploitation and Utilization in Sichuan Higher Education.
Resumo: Background: Cinnamomum longepaniculatum is an important commercial crop and the main source of volatile terpenoids. The biosynthesis of key bioactive metabolites of C. longepaniculatum is not well understood because of the lack of available genomic and transcriptomic information. To address this issue, we performed transcriptome sequencing of C. longepaniculatum leaves to identify factors involved in terpenoid metabolite biosynthesis. Results: Transcriptome sequencing of C. longepaniculatum leaves generated over 56 million raw reads. The transcriptome was assembled using the Trinity software and yielded 82,061 unigenes with an average length of 879.43 bp and N50 value of 1387 bp. Furthermore, Benchmarking Universal Single-Copy Orthologs analysis indicated that our assembly is 91% complete. The unigenes were used to query the nonredundant database depending on sequence similarity; 42,809 unigenes were homologous to known genes in different species, with an annotation rate of 42.87%. The transcript abundance and Gene Ontology analyses revealed that numerous unigenes were associated with metabolism, while others were annotated in functional categories including transcription, signal transduction, and secondary metabolism. The Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that 19,260 unigenes were involved in 385 metabolic pathways, with 233 unigenes found to be involved in terpenoid metabolism. Moreover, 23,463 simple sequence repeats were identified using the microsatellite identification tool. Conclusion: This is the first detailed transcriptome analysis of C. longepaniculatum. The findings provide insights into the molecular basis of terpenoid biosynthesis and a reference for future studies on the genetics and breeding of C. longepaniculatum.
Descritores: Terpenos/metabolismo
Sequenciamento de Nucleotídeos em Larga Escala
-Transcrição Genética
Óleos Voláteis/metabolismo
Repetições de Microssatélites
Anotação de Sequência Molecular
Ontologia Genética
Responsável: CL1.1 - Biblioteca Central

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Id: lil-741294
Autor: Li, Li; Shi, Chaofeng; Yin, Zhongqiong; Jia, Renyong; Peng, Lianci; Kang, Shuai; Li, Zhengwen.
Título: Antibacterial activity of α-terpineol may induce morphostructural alterations in Escherichia coli
Fonte: Braz. j. microbiol;45(4):1409-1413, Oct.-Dec. 2014. ilus, graf.
Idioma: en.
Projeto: Sichuan Youth Science and Technology Innovation Research Team; . National Natural Science Foundation of China.
Resumo: The antibacterial effect of α-terpineol from Cinnamomum longepaniculatum (Gamble) N. Chao leaf essential oils were studied with special reference to the mechanism of inhibiting the standard strain of Escherichia coli (CMCC (B) 44102) growth at ultrastructural level. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) and time-kill curves of α-terpineol were determined; Escherichia coli was treated with α-terpineol and observed under a transmission electron microscope. The MIC and MBC values of α-terpineol were all 0.78 µL/mL, and time-kill curves showed the concentration-dependent. Under the transmission electron microscopy (TEM), Escherichia coli exposed to MIC levels of α-terpineol exhibited decreased cell size and irregular cell shape, cell wall and cell membrane were ruptured, nucleus cytoplasm was reduced and nuclear area gathered aside. Results suggest that α-terpineol has excellent antibacterial activity and could induce morphological changes of Escherichia coli.
Descritores: Antibacterianos/farmacologia
Escherichia coli/citologia
Escherichia coli/efeitos dos fármacos
-Membrana Celular/ultraestrutura
Núcleo Celular/ultraestrutura
Parede Celular/ultraestrutura
Cicloexenos/isolamento & purificação
Testes de Sensibilidade Microbiana
Microscopia Eletrônica de Transmissão
Viabilidade Microbiana/efeitos dos fármacos
Monoterpenos/isolamento & purificação
Óleos Voláteis/isolamento & purificação
Folhas de Planta/química
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME

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