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Id: biblio-1253093
Autor: Umer, Noroza; Zahra Naqvi, Rubab; Rauf, Imran; Anjum, Naveed; Keen, Patricia R; Van Eck, Joyce; Jander, Georg; Asif, Muhammad.
Título: Expression of Pinellia ternata leaf agglutinin under rolC promoter confers resistance against a phytophagous sap sucking aphid, Myzus persicae
Fonte: Electron. j. biotechnol;47:72-82, sept. 2020. tab, ilus, graf.
Idioma: en.
Projeto: International Research Support Initiative Program of Higher Education Commission (HEC) of; . Pakistan; . United States Department of Agriculture (USDA).
Resumo: BACKGROUND: Piercing/sucking insect pests in the order Hemiptera causes substantial crop losses by removing photoassimilates and transmitting viruses to their host plants. Cloning and heterologous expression of plantderived insect resistance genes is a promising approach to control aphids and other sap-sucking insect pests. While expression from the constitutive 35S promoter provides broad protection, the phloem-specific rolC promoter provides better defense against sap sucking insects. The selection of plant-derived insect resistance genes for expression in crop species will minimize bio-safety concerns. RESULTS: Pinellia ternata leaf agglutinin gene (pta), encodes an insecticidal lectin, was isolated and cloned under the 35S and rolC promoters in the pGA482 plant transformation vector for Agrobacterium-mediated tobacco transformation. Integration and expression of the transgene was validated by Southern blotting and qRT-PCR, respectively. Insect bioassays data of transgenic tobacco plants showed that expression of pta under rolC promoter caused 100% aphid mortality and reduced aphid fecundity up to 70% in transgenic tobacco line LRP9. These results highlight the better effectivity of pta under rolC promoter to control phloem feeders, aphids. CONCLUSIONS: These findings suggested the potential of PTA against aphids and other sap sucking insect pests. Evaluation of gene in tobacco under two different promoters; 35S constitutive promoter and rolC phloemspecific promoter could be successfully use for other crop plants particularly in cotton. Development of transgenic cotton plants using plant-derived insecticidal, PTA, would be key step towards commercialization of environmentally safe insect-resistant crops.
Descritores: Afídeos/patogenicidade
Controle Biológico de Vetores
Pinellia/química
-Vírus de Plantas
Tabaco
Southern Blotting
Reação em Cadeia da Polimerase
Regiões Promotoras Genéticas
Plantas Geneticamente Modificadas
Folhas de Planta/química
Transgenes
Resistência à Doença
Proteção de Cultivos
Responsável: CL1.1 - Biblioteca Central


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Id: lil-448073
Autor: Lin, Juan; Zhou, Xuanwei; Fei, Jiong; Liao, Zhihua; Jin, Wang; Sun, Xiaofen; Tang, Kexuan.
Título: Genomic cloning and characterization of a PPA gene encoding a mannose-binding lectin from Pinellia pedatisecta
Fonte: Biocell;30(1):15-25, abr. 2006. ilus, tab.
Idioma: en.
Resumo: A gene encoding a mannose-binding lectin, Pinellia pedatisecta agglutinin (PPA), was isolated from leaves of Pinellia pedatisecta using genomic walker technology. The ppa contained an 1140-bp 5'-upstream region, a 771-bp open reading frame (ORF) and an 829-bp 3'-downstream region. The ORF encoded a precursor polypeptide of 256 amino acid residues with a 24-amino acid signal peptide. There were one putative TATA box and six possible CAAT boxes lying in the 5'-upstream region of ppa. The ppa showed significant similarity at the nucleic acid level with genes encoding mannose-binding lectins from other Araceae species such as Pinellia ternata, Arisaema heterophyllum, Colocasia esculenta and Arum maculatum. At the amino acid level, PPA also shared varying homology (ranging from 40% to 85%) with mannose-binding lectins from other plant species, such as those from Araceae, Alliaceae, Iridaceae, Lillaceae, Amaryllidaceae and Bromeliaceae. The cloning of the ppa gene not only provides a basis for further investigation of PPA's structure, expression and regulation mechanism, but also enables us to test its potential role in controlling pests and fungal diseases by transferring the gene into tobacco and rice in the future
Descritores: Clonagem Molecular
DNA de Plantas
Genes de Plantas/genética
Lectina de Ligação a Manose/genética
Conformação Proteica
Pinellia/genética
-Dados de Sequência Molecular
Lectinas de Plantas
Responsável: AR40.1 - Biblioteca de la Facultad de Ciencias Médicas de la UNCuyo



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