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Id: lil-668693
Autor: Geum-Hwa, Lee; Hyung-Ryong, Kim; Sang-Yong, Han; Bidur, Bhandary; Do-Sung, Kim; Min-Gul, Kim; Byung-Ok, So; Sun-Young, Kim; Kyu-Sik, Jo; Bo-Hee, Lee; Hee-Nam, Seo; Soo-Wan, Chae; Han-Jung, Chae.
Título: Gastrodia elata Blume and its pure compounds protect BV-2 microglial-derived cell lines against β-amyloid: The involvement of GRP78 and CHOP
Fonte: Biol. Res;45(4):403-410, 2012. ilus.
Idioma: en.
Projeto: funding from the MooJoo Hyangto Research Group.
Resumo: Objectives: Gastrodia elata (GE) Blume (Orchidaceae) has been previously known for its therapeutic benefits against neurodegenerative diseases. Microglial activation and death have been implicated in the pathogenesis of a variety of neurodegenerative diseases, including Alzheimer's disease. In this study, GE and its pure components, gastrodin and 4-hydroxybenzyl alcohol (4HBA), were applied to β-amyloid-induced BV2 mouse microglial cells. Materials and Methods Cell viability was assessed by the MTT assay and Western blotting was also performed. Results: β-amyloid-induced cell death was shown to be induced time- and dose-dependently. To examine the cell death mechanism, we confirmed the involvement of ER stress signaling. C/EBP homologous protein (CHOP), a pro-apoptotic ER stress protein, was expressed at high levels but glucose-regulated protein 78 (GRP78), an anti-apoptotic ER stress protein with chaperone activity, was only slightly affected by treatment with β-amyloid. However, pretreatment with GE and its components inhibited the expression of CHOP but increased that of GRP78 in β-amyloid-treated cells. This study also showed that a single treatment with GE extracts, gastrodin, or 4HBA induced the expression of GRP78, a marker for enhanced protein folding machinery, suggesting a protective mechanism for GE against β-amyloid. Conclusions: This study reveals the protective effects of GE against β-amyloid-induced cell death, possibly through the enhancement of protein folding machinery of a representative protein, GRP78, and the regulation of CHOP in BV2 mouse microglial cells.
Descritores: Amiloide/farmacologia
Álcoois Benzílicos/farmacologia
Morte Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Microglia/efeitos dos fármacos
-Álcoois Benzílicos/isolamento & purificação
Glucosídeos/isolamento & purificação
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central

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