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Id: biblio-1128573
Autor: Martinez, Renata Micheli; Souza, Clara Ruiza; Melo, Cristina de Paula Barros; Baracat, Marcela Maria; Arakawa, Nilton Syogo; Duarte, José Carlos; Verri Jr, Waldiceu Aparecido; Casagrande, Rúbia; Georgetti, Sandra Regina.
Título: Prevention of UVB radiation-induced oxidative stress in mice by topical administration of Azadirachta indica(neem) extract
Fonte: Rev. ciênc. farm. básica apl;41:[14], 01/01/2020. tab, ilus, graf.
Idioma: en.
Resumo: Neem tree (Azadirachta indica A. Juss. fam. Meliaceae) has been extensively employed to combat diverse pathologies. Moreover, it has been described that its leaf extract present anticarcinogenic action. Thus, the neem extract (NE) chemical and antioxidant properties was evaluated, and also, the capacity of two dermatological formulations incorporated with neem extract (F1 and F2) to avoid oxidative UVB-induced skin injury in hairless mice. NE constituents were investigated and free radical scavenging ability were determined by different methods in vitro. Skin from mice treated with F1 and F2 and submitted to UVB radiation were tested for different parameters of inflammation and oxidative injury. Results show that the NE polyphenol and flavonoid content were 135.30 and 37.12mg/g, respectively. High performance liquid chromatography (HPLC) results demonstrated the existence of azarachtin, rutin, ursolic acid and tannic acid. NE presented scavenging ability by ABTS radical, ferric-reducing antioxidant power (FRAP), inhibition of lipid peroxidation and iron chelation. In vivo, it was observed that mice treated with F1 and F2 showed amelioration of the inflammation by reducing UVB induced skin edema. However, only samples from animals treated with F1 had lower neutrophil recruitment (measured by myeloperoxidase activity), and returning the oxidative status to baseline levels in parameters such as reduced glutathione level, ferric reducing ability (FRAP), and scavenging of free radical (ABTS). Concluding, NE demonstrated a good antioxidant property in vitro, and the data suggest the use of NE added F1 to prevent skin damage caused by UVB irradiation.(AU)
Descritores: Raios Ultravioleta/efeitos adversos
Estresse Oxidativo/efeitos dos fármacos
Azadirachta
Antioxidantes/efeitos da radiação
-Administração Cutânea
Cromatografia Líquida de Alta Pressão/métodos
Camundongos
Limites: Animais
Masculino
Feminino
Camundongos
Responsável: BR33.1 - Divisão Técnica de Biblioteca e Documentação


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Id: lil-623740
Autor: Garcia, Eloi S; Feder, Denise; Gomes, José Eugénio P. L; Azambuja, Patricia de.
Título: Effects of precocene and azadirachtin in Rhodnius prolixus: some data on development and reproduction
Fonte: Mem. Inst. Oswaldo Cruz;82(supl.3):67-73, 1987. graf, tab.
Idioma: en.
Conferência: Apresentado em: International Symposium on Insect Physiology, Biochemistry and Control, Rio de Janeiro, Nov. 10-13, 1987.
Resumo: The results presented in this paper clearly indicate that precocene and azadirachtin are effective inhibitors of moulting and reproduction in the hemipteran Rhodnius prolixus. The time of application is important and only applications of these substances early in the intermoulting period cause their effects in nymphs. The inhibition of moulting is fully reversed by ecdysone therapy. Precocene and azadirachtin also affected drastically the oogenesis and egg deposition in this insect. Precocene-induced sterilization is reversed by application of juvenile hormone III. However, this hormone is unable to reverse the effect of azadirachtin on reproduction. Ecdysteroid titers in nymphs and adult females are decreased by these treatments. In vitro analysis suggest that precocene and azadirachtin may act directly on the prothoracic glands and ovaries producing ecdysteroids. Based on these and other findings the possible mode of action of these compounds on the development and reproduction of Rhodnius prolixus is discussed.
Descritores: Farmacologia/estatística & dados numéricos
Azadirachta
-Rhodnius
Responsável: BR1.1 - BIREME


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Id: lil-623739
Autor: Rembold, Heinz.
Título: The Azadirachtins: potent insect growth inhibitors
Fonte: Mem. Inst. Oswaldo Cruz;82(supl.3):61-66, 1987. ilus.
Idioma: en.
Conferência: Apresentado em: International Symposium on Insect Physiology, Biochemistry and Control, Rio de Janeiro, Nov. 10-13, 1987.
Resumo: In the course of their coevolution with insects, plants have learnt to protect themselves by chemical means. Semiochemical act as antifeedants or deterrents, others by disrupting growth and development. By use of the Epilachna varivestis bioassay we isolated from Azadirachta indica seed a group of triterpenoids which interfee with larval growth and development in ppm range. Main components are the azadirachtins A and B with identical biological activity. Various other azadirachtins were obtained, either as minor seed components or by chemical modification of the naturally occuring compounds. Structure vs. activity relation studies enabled us to postulate a basic structural element that should still be biologically active and with much simpler chemical structure than natural compounds. What underlies the biological activity of these insect growth inhibitors? Their interference with the hormonal regulation of development and reproduction has been studied in Locusta migratoria and Rhodnius prolixus. In addition, tritiated dihydroazadirachtin A was used. With this approach, a precise correlation between administered dose, resulting effects, and retention of the compound was established. The azadirachtins either interrupt, delay, or deviate whole developmental programs. Results from these studies provide another chemical probe for studies in insect endocrinology and physiology.
Descritores: Inibidores do Crescimento/análise
Inibidores do Crescimento/uso terapêutico
-Azadirachta
Responsável: BR1.1 - BIREME


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Id: biblio-1004998
Autor: Coelho Junior, Aloisio; Deschamps, Francisco Carlos.
Título: Ação sistêmica e translaminar do óleo de nim visando ao controle de Tuta absoluta (Meyrick) (Lep: Gelechiidae) em tomateiro / Systemic and translaminar action of neem oil for the control of Tuta absoluta (Meyrick) (Lep: Gelechiidae) in tomato
Fonte: Arq. Inst. Biol;81(2):140-144, abr.-jun. 2014. tab.
Idioma: pt.
Projeto: Embrapa/ Fundagro/ Epagri/ UFSC. projeto Prodetab.
Resumo: A traça Tuta absoluta (Meyrick) é o inseto-praga que mais compromete a produção do tomateiro. Basear o seu controle no uso de inseticidas sintéticos pode comprometer a cultura em termos econômicos e ambientais. Como alternativa de controle, o óleo de nim foi aplicado em folhas de tomateiro, avaliando os efeitos inseticida e antialimentar por ação sistêmica e translaminar. No ensaio para avaliar a ação translaminar, o óleo diluído em água foi aplicado na superfície adaxial dos folíolos, em concentrações de 0,5, 1, 5 e 10%. A mortalidade observada variou entre 49 e 100%, e o tamanho médio das minas, entre 1,62 e 0,34 cm. Nos tratamentos representados por água e água + emulsificante (5%), a mortalidade não ultrapassou 20%, com tamanho médio das minas de 1,63 cm. O estudo da ação sistêmica foi realizado ao aplicar o óleo de nim na superfície adaxial e abaxial de todas as folhas das plantas, à exceção daquelas em que as lagartas foram infestadas. Neste ensaio, o óleo foi aplicado nas concentrações de 1, 2,5 e 5%, diluídos em água e emulsificante. Observou-se que a mortalidade foi maior que 90% quando o óleo foi aplicado nas concentrações de 2,5 e 5%, sendo o tamanho médio das minas inferior a 1,0 cm. Não ocorreu mortalidade nos tratamentos controle, mas o tamanho das minas foi, em média, de 1,96 cm. Desta maneira ficou evidenciado que o óleo de nim apresenta efeito inseticida e antialimentar sobre lagartas de T. absoluta, com a propriedade de ser incorporado e translocado através das folhas de tomateiro.(AU)

The leaf miner Tuta absoluta (Meyrick) is the pest that mostly jeopardizes tomato production. The use of synthetic pesticides for its control can affect the crop economically and environmentally. As an alternative form of control, neem oil was applied on tomato leaves in order to assess antifeedant and insecticidal effects by systemic and translaminar action. In experiment for translaminar action, oil diluted in water was applied to the adaxial surface of leaflets at concentrations of 0.5, 1, 5 and 10%. The mortality rate ranged between 49 and 100%, and the average size of the mines, between 1.62 and 0.34 cm. In treatments represented by water and water + emulsifier (5%), mortality did not surpass 20%, with the average size of mines reaching 1.63 cm. The study of systemic action was performed by applying neem oil to the adaxial and abaxial surfaces of all leaves, except those that were infested by caterpillars. In this assay, the oil was applied at concentrations of 1, 2.5 and 5%, being diluted in water and emulsifiers. We observed that the mortality rate exceeded 90% when the oil was applied at concentrations of 2.5 and 5%, and the average size of mines was smaller than 1.0 cm. There was no mortality in the control treatments, but the mine size was on average 1.96 cm. Therefore, it is clear that the neem oil has an insecticidal and anti-food effect on T. absoluta caterpillars, and is possible to be incorporated and applied on tomato leaves.(AU)
Descritores: Lycopersicon esculentum
Azadirachta
Lepisma
Inseticidas
-Controle de Pragas
Insetos
Responsável: BR1942.1 - NID - Biblioteca - Núcleo de Informação e Documentação


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Id: biblio-989374
Autor: Li, L; Song, X; Yin, Z; Jia, R; Zou, Y.
Título: Insecticidal activities and mechanism of extracts from neem leaves against Oxya chinensis / Atividade e mecanismos inseticidas de extratos de folhas "neem" contra Oxya chinesis
Fonte: Arq. bras. med. vet. zootec. (Online);71(1):1-10, jan.-fev. 2019. tab, graf.
Idioma: en.
Projeto: Sichuan Province; . Sichuan Youth Science and Technology Innovation Research Team; . National Natural Science Foundation of China.
Resumo: In this study, neem leaves were successively extracted with petroleum ether, 95% ethanol and water and the insecticidal activities of these extracts against Oxya chinensis larvae were measured. The results showed that 95% ethanol extract gave the highest extraction yield and insecticidal activity, and it was further extracted with five different solvents. The petroleum ether extract from the 95% ethanol extract possessed the highest insecticidal activity with median lethal concentration values ranging from 14.93 to 55.66mg/mL. The gas chromatography-mass spectrometer analysis showed that the petroleum ether extract mainly composed of alkanes, olefin, esters and amide. The pathological examination revealed that the prominent lesions, including reduced regenerative cells in midgut and swelled and degenerated cylindrical cells, were observed in the 5th instar Oxya chinensis after treatment. The ultrastructural features showed that the cylindrical cells, microvilli and mitochondria were seriously damaged. These results suggested that the petroleum ether extract from neem leaves had potent insecticidal activity and could be a candidate insecticide.(AU)

Nesse estudo, folhas "neem" foram extraídas com sucesso com éter de petróleo, 95% de etanol e água, e as atividades inseticidas desses extratos foram medidas contra larvas de Oxya chinesis. Os resultados mostram que extrato com 95% de etanol deram o maior resultado de extração e atividade inseticida e foi então extraído utilizando mais cinco diferentes solventes. O éter de petróleo do extrato de 95% etanol apresentou maior atividade inseticida com concentração letal média variando de 14.93 a 55.66mg/mL. A análise por cromatografia de massa mostrou que o extrato de éter de petróleo está composto principalmente de alcanos, alcenos, ésteres e amidas. A avaliação patológica revelou que as lesões proeminentes, inclusive células regenerativas reduzidas no intestino e células cilíndricas edemaciadas e degeneradas foram observadas no quinto estágio de desenvolvimento da Oxya chinesis após tratamento. As características ultraestruturais mostraram que as células cilíndricas, microvilos e mitocôndrias apresentavam lesões graves. Esses resultados sugerem que o extrato de éter de petróleo de folhas de "neem" tem atividade inseticida potente e pode ser um candidato a inseticida.(AU)
Descritores: Azadirachta/fisiologia
Inseticidas/análise
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: biblio-1023472
Autor: Ramírez Mérida, Luis Guillermo; Morón de Salim, Alba; Reyes, Doris; Rivero, Ariana; Sánchez, Luz A; Sánchez, Luz K.
Título: Antimicrobial effect of ethanol extract of leaf neem (Azadirachta indica A. Juss) on Listeria monocytogenes
Fonte: Arch. latinoam. nutr;66(1):60-65, mar. 2016. graf.
Idioma: es.
Resumo: Listeria monocytogenes es un patógeno causante de enfermedades alimentarias. En la búsqueda de controlar su propagación utilizando sustancias naturales se planteó el objetivo de mostrar si el extracto etanólico foliar de neem (Azadirachta Indica A. Juss.) tiene efecto antimicrobiano sobre L. monocytogenes ICTA-12446. El extracto se obtuvo a partir de hojas de neem sometidas a secado por 8 días, se redujeron de tamaño mecánicamente, se sometieron a maceración en frío por 3 días usando etanol 96% en recipientes ámbar, se filtró y concentró en rota evaporador. Se estandarizó el concentrado con dimetilsulfóxido (DMSO) a una concentración de 60 mg/L. Listeria monocytogenes ICTA-12446, fue inoculado en caldo nutriente junto con soluciones del extracto a diferentes concentraciones (20, 30, 40, 50 y 60 mg/L), se emplearon tiempos de contacto (2.5, 5, 10 y 15 minutos). Cumplido cada tiempo se realizaron diluciones seriadas e inocularon en agar nutritivo por extensión durante 24 h a 37ºC. Se efectuó el recuento en Unidades Formadoras de Colonias UFC. Al comparar las concentraciones del extracto se evidencia entre 20 y 60 mg/mL diferencia significativa, mientras que en 30, 40 y 50 mg/mL un comportamiento similar. Al contrastar tiempos de contacto, se observa que entre el tiempo 2.5 min y los restantes un p=0,03. El tiempo mínimo donde existió inhibición fue 2.5 minutos, y concentración mínima inhibitoria de 20 mg/mL. Los cuatro tiempos de contacto arrojan porcentajes de inhibición microbiana de 100% al emplear 60mg/mL. Se concluye que el extracto etanólico foliar de neem posee un efecto inhibitorio sobre Listeria monocytogenes(AU)

Listeria monocytogenes is a pathogen causing foodborne illness. In seeking to control its spread using natural substances in order to show if the leaf ethanol extract of neem (Azadirachta indica A. Juss) has antimicrobial effect on L. monocytogenes ICTA-12446, was proposed. The extract was obtained from neem leaves, which was subjected to drying for 8 days. It was reduced in size mechanically, and subjected to cold soak for 3 days, using 96% ethanol in amber vessels, filtered and concentrated in rot evaporator. Concentrated was solubilized with dimethylsulfoxide (DMSO) and standarized to achieve a concentration of 60 mg/mL Listeria monocytogenes was inoculated in nutrient broth with extract solutions at different concentrations (20, 30, 40, 50 and 60mg/mL), four contact times (2.5, 5, 10 and 15 minutes) were used. Completed each time it was diluted and inoculated on nutrient agar by extension for 24h at 37ºC. The count of Colony Forming Units UFC was taking. Comparing the concentrations of the extract between 20 and 60mg /mL significant difference was appreciate, while 30, 40 and 50 mg/mL show a similar behavior. Contrasting contact times observed between time 2.5 min and the remaining p = 0.03. The minimum time where there was some kind of inhibition was 2.5 minutes, and minima inhibitory concentration of 20mg/mL. The four contact times yield microbial inhibition percentages of 100% by using 60mg/L. It is concluded that ethanol extract of neem leaf has an inhibitory effect on L. monocytogenes(AU)
Descritores: Bacilos Gram-Negativos Anaeróbios Facultativos/fisiologia
Azadirachta/fisiologia
Etanol/química
Manipulação de Alimentos/métodos
Listeria monocytogenes
-Bacteriologia
Efeitos Fisiológicos de Drogas
Limites: Humanos
Masculino
Feminino
Tipo de Publ: Estudo de Avaliação
Responsável: VE557.1 - Biblioteca Fundación Bengoa


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Id: biblio-948818
Autor: Mesquita, Francisco Oliveira; Cavalcante, Lourival Ferreira; Nunes, Járisson Cavalcante; Souto, Antonio Gustavo Luna; Medeiros, Reinaldo Ferreira; Rodrigues, Rummenigge Macêdo.
Título: Formação de mudas de Nim com aplicação de biofertilizante bovino submetido à drenagem e estresse salino / Formation of Neem seedlings with application of bovine biofertilizer subjected to drainage and saline stress
Fonte: Biosci. j. (Online);31(1):47-54, jan./fev. 2015.
Idioma: pt.
Resumo: Diversos fatores podem influenciar a formação de mudas. Entre as causas de insucesso, encontram-se a dificuldade de manutenção de drenagem e qualidade da água de irrigação. O objetivo do trabalho foi avaliar os efeitos do biofertilizante bovino e salinidade da água de irrigação na formação de mudas de nim. O delineamento experimental foi em blocos casualizados, com os tratamentos arranjados no esquema fatorial 5 x 2 x 2, com quatro repetições, referente aos níveis de salinidade da água de irrigação de 0,5; 1,5; 3,0; 4,5 e 6,0 dS m-1, no solo sem e com biofertilizante bovino, sem e com drenagem. As variáveis analisadas foram emergência de plântulas, número de folhas, diâmetro caulinar e área foliar. O biofertilizante bovino depois de diluído em água não salina (0,49 dS m-1) e não clorada na razão de 1:1, foi aplicado uma única vez, dois dias antes da semeadura, ao nível de 10% do volume do substrato. A irrigação das plantas com cada tipo de água foi feita diariamente fornecendo um volume de água suficiente para elevar a umidade do solo para o nível mais próxima da capacidade de campo. Pelos resultados, com o aumento da salinidade das águas houve reduções no crescimento das plantas em até 28%, mas o biofertilizante bovino e a drenagem do solo proporcionaram melhores condições de crescimento das mudas.

Several factors may influence in formation of seedlings. Among the causes of failure find discovered the difficulty of maintain of the drainage and quality of irrigation water. The objective of wok was study or evaluate the effects of bovine biofertilizer and water salinity in the formation of neem seedlings. The experimental design was in randomized block, with the treatments arranged in factorial scheme 5 x 2 x 2, with four repetitions, referring at the five salinity levels of irrigation water: 0.5, 1.5, 3.0, 4.5 and 6.0 dS m-1, in soil without and with bovine biofertilizer, in soil without and with soil drainage. The variables analyzed were: seedlings emergence, number of leaves, stem diameter and leaf area. The bovine biofertilizer after diluted in noon-saline water (0,49 dS m-1) e not chlorinated in reason to 1:1, was applied an once two days before sowing, at level to 10% of substrate volume. The plants were irrigated daily with each type of water in volume sufficient to increase the soil moisture for the level nearest of field capacity. By results, with the increasing of salinity waters there were reductions in plant growth up to 28%, but the bovine biofertilizer and soil drainage provided better conditions for seedling growth.
Descritores: Solo
Águas Salinas
Drenagem do Solo
Azadirachta
Plântula
Fertilização
Responsável: BR396.1 - Biblioteca Central


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Id: biblio-965291
Autor: Vilarinho, Marcella Karoline Cardoso; Silva, Tonny José Araújo da; Caneppele, Carlos; Rozado, Adriano Ferreira.
Título: Chemical pesticides and vegetal extracts on Sitophilus zeamais control in stored corn grains / Inseticidas químicos e extratos vegetais no controle de Sitophilus zeamais em grãos de milho armazenados
Fonte: Biosci. j. (Online);32(2):288-297, mar./abr. 2016. ilus, tab.
Idioma: en.
Resumo: Sitophilus species are major pests of stored grain and their control is achieved mainly with the use of chemical insecticides, but the indiscriminate use of these products is resulting in several undesirable factors to man and to the environment. Thus, the use of natural insecticides comes as an option to control the insects, while lessening risks to the environment. The study was conducted at the Institute of Agricultural Sciences and Technologies, Rondonópolis campus of the Federal University of Mato Grosso, in the period from March to September 2012. The experiment was conducted under three different storing conditions. Aqueous extracts were obtained by the addition of Allium sativum L, Azadirachta indica A. Juss. and Cymbopogon winterianum Jowitt vegetable powders in distilled water, at a ratio of 5 g per 100 ml, and the levels of chemical insecticides were of 0.04 and 0.15 ml/100 ml of water for deltamethrin and chlorpyrifos, respectively. Treatments were added to the corn grains, which were placed in a 2.5 L glass container, mixed by manual shaking and infested with 20 adults of unsexed Sitophilus zeamais. Grains were stored for 60 days. At 30 and 60 days, the following items were analyzed: bugs count, water content in grains and electrical conductivity. The data were submitted to analysis of variance, and means were compared by Tukey test at 5% probability. At 30 days, the efficiency of chemical insecticides in the control of Sitophilus zeamais was observed in the three storage environments. Vegetal extracts were not effective in controlling insects. The larger number of insects increased the electrical conductivity and humidity values in the grains.

O objetivo desse trabalho foi estudar o efeito inseticida de extratos vegetais aquosos e inseticidas químicos sob condições de armazenamento. O experimento foi realizado sob três condições de armazenamento. Os extratos aquosos foram obtidos pela adição dos pós vegetais de Allium sativum L, Azadirachta indica A. Juss. e Cymbopogon winterianum Jowitt. em água destilada na proporção de 5g por 100 ml, e as dosagens dos inseticidas químicos foram de 0,04 e 0,15 ml/100 ml de água para Deltametrina e Clorpirifós respectivamente. Os tratamentos foram adicionados aos grãos de milho acondicionados em recipientes de vidro de 2,5 L, misturados por agitação manual, e infestados com 20 adultos de Sitophilus zeamais não sexados. Os grãos ficaram armazenados durante 60 dias. Analisou-se aos 30 e 60 dias: contagem de insetos, teor de água nos grãos e condutividade elétrica. Os dados foram submetidos à analise de variância, e as médias comparadas pelo teste de Tukey a 5 % de probabilidade. Aos 30 dias, observa-se eficiência dos inseticidas químicos no controle de Sitophilus zeamais nos três ambientes de armazenamento. Os extratos vegetais não são eficientes no controle dos insetos. O maior número de insetos elevam os valores de condutividade elétrica e umidade nos grãos.
Descritores: Temperatura
Azadirachta
Cymbopogon
Alho
Inseticidas
Insetos
Responsável: BR396.1 - Biblioteca Central


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Id: biblio-887869
Autor: Schneider, Larissa Carla Lauer; Silva, Camila Vieira da; Conte, Hélio.
Título: Toxic effect of commercial formulations of neem oil, Azadirachta indica A. Juss., in pupae and adults of the sugarcane borer, Diatraea saccharalis F. (Lepidoptera: Crambidae) / Efeito tóxico de formulações comerciais de óleo de neem, Azadirachta indica A. Juss., em pupas e adultos da broca da cana-de-açúcar, Diatraea saccharalis F. (Lepidoptera: Crambidae)
Fonte: Arq. Inst. Biol;84:e0432014, 2017. tab, ilus.
Idioma: en.
Resumo: To evaluate the toxic effect of commercial formulations of neem oil, Azadirachta indica A. Juss, pre-pupae (PP), young pupae (YP) and old pupae (OP) of Diatraea saccharalis F. (Lepidoptera: Crambidae) were sprayed with the diluted extract in distilled water at concentrations of 0.0, 0.3, 0.5, 1.0 and 2.0%. The neem extract caused concentration-dependent effects on mortality of pupae, and the pupae that failed to emerge in adults had multiple abnormalities. The longevity of pupae that emerged in adults (YP and OP group) did not differ from the control group. The abnormalities found in adults were related to mortality in all treatments, except at the concentration of 1.0%. Fertility was assessed according to the oviposition of adult females from the YP group that showed no abnormalities, through the evaluation of the number of deposited eggs and the rate of undeveloped eggs. The results showed a reduction in the number of eggs laid and an increase in the percentage of undeveloped eggs. These results show that neem oil has a high potential to control the toughest stage of the sugarcane borer and also reduces the further development. Therefore, commercial formulations of neem oil have a toxic effect on pupae and adults of D. saccharalis.(AU)

Para avaliar o efeito tóxico de formulações comerciais de óleo de neem, Azadirachta indica A. Juss, pré-pupas (PP), pupas jovens (PJ) e pupas velhas (PV) da Diatraea saccharalis F. (Lepidoptera: Crambidae) foram pulverizadas com o extrato diluído em água destilada, em concentrações de 0,0, 0,3, 0,5, 1,0 e 2,0%. O neem provocou diferentes efeitos sobre a mortalidade de pupas, dependendo da concentração. As pupas que não conseguiram emergir em adultos apresentaram anormalidades múltiplas. Quanto às pupas que emergiram em adultos (grupos PJ e PV), foi calculada a sua longevidade, que não diferiu da do grupo controle. As anormalidades encontradas em adultos estão relacionadas com a mortalidade em todos os tratamentos com exceção da concentração de 1,0%. A fecundidade foi avaliada de acordo com a oviposição de adultos fêmeas do grupo PJ, que não apresentaram anormalidades; dentro dos ovos depositados foi avaliado o número de ovos não desenvolvidos. Os resultados demonstraram redução no número de ovos depositados e aumento na porcentagem de ovos não desenvolvidos. Esses resultados mostraram que o óleo de neem tem elevado potencial para o controle do estágio mais resistente da broca da cana-de-açúcar, além de reduzir o aparecimento das fases subsequentes. Portanto, formulações comerciais de óleo de neem apresentam um efeito tóxico em pupas e adultos de D. Saccharalis.(AU)
Descritores: Azadirachta
Saccharum
Toxicidade
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Id: lil-727603
Autor: Fong Lores, Onel; Berenguer Rivas, Clara; de la Vega Acosta, Jorge; Wawoe Díaz, Nioslaymy; Puente Zapata, Edgar.
Título: Potencial antioxidante de un extracto acuoso de hojas del NIM (Azadirachta Indica A. Juss) / Antioxidant potential of an aqueous leaf extract of Neem (Azadirachta Indica A. Juss)
Fonte: Rev. cuba. plantas med;19(2):205-207, Apr.-June 2014. tab, Ilus.
Idioma: es.
Resumo: INTRODUCCIÓN: la planta medicinal Azadirachta indica A. Juss conocida ancestralmente como el Árbol del Nim ha sido empleada desde la antigüedad para el tratamiento de múltiples afecciones, sin embargo, es en la actualidad con el desarrollo tecnológico que se han realizado las investigaciones científicas de sus propiedades terapéuticas. OBJETIVO: evaluar el potencial antioxidante de los extractos acuosos de hojas del Árbol del Nim. MÉTODOS: el extracto de las hojas secas del Nim se obtuvo empleando un equipo Soxhlet y fue caracterizado mediante un análisis fitoquímico preliminar y espectroscopia UV/VIS. El contenido de fenoles totales fue determinado por el ensayo de Folin-Ciocalteu y la determinación de la actividad antioxidante fue desarrollada por el método del reactivo de fosfomolibdeno. RESULTADOS: el espectro UV/VIS del extracto acuoso obtenido exhibe un máximo de absorción a las longitudes de onda 217 y 245 nm, característicos de los principios activos de la planta (azadiractina y nimbina). El contenido de fenoles del extracto de la planta expresados en equivalentes de ácido tánico fue de 54,87 mg/g y mostró una actividad antioxidante a través del ensayo del fosfomolibdeno (215,01 mmol/g), comparable al ácido ascórbico. CONCLUSIONES: los resultados obtenidos demuestran que las hojas Árbol del Nim son una fuente rica en compuestos fenólicos con propiedades antioxidantes y un candidato potencial para el desarrollo de nuevos fitofármacos.

INTRODUCTION: the medicinal plant Azadirachta indica A. Juss, traditionally known as neem tree, has been used since ancient times to treat numerous conditions. However, it is only at present and thanks to current technological development that scientific research has been conducted into its therapeutic properties. OBJECTIVE: evaluate the antioxidant potential of aqueous leaf extracts of the neem tree. METHODS: extract from neem dry leaves was obtained with a Soxhlet device and characterized by preliminary phytochemical analysis and UV/VIS spectroscopy. Total phenol content was determined by the Folin-Ciocalteu assay, and antioxidant activity by the phosphomolybdenum method. RESULTS: the UV/VIS spectrum of the aqueous extract exhibited an absorption peak at wavelengths of 217 and 245 nm, characteristic of the active principles in the plant (azadirachtin and nimbin). Phenol content expressed as tannic acid equivalents was 54.87 mg/g, revealing an antioxidant activity in the phosphomolybdenum assay of 215.01 mol/g, comparable to that of ascorbic acid. CONCLUSIONS: the results obtained show that neem leaves are a rich source of phenolic compounds with antioxidant properties, and a potential candidate for the development of new phytomedicines.
Descritores: Preparações de Plantas/uso terapêutico
Azadirachta
Compostos Fenólicos
Antioxidantes/uso terapêutico
Responsável: CU1.1 - Biblioteca Médica Nacional



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