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Id: biblio-910578
Autor: Tonelli, Mateus; Geromel, Mairto Roberis; Fazio, Maria Luiza Silva.
Título: Ação antimicrobiana de óleos essenciais de sucupira branca (Pterodon emarginatus); folhas de pêssego (Prunus persica); bagas de junipero (Juniperus communis); rosa de damasco (Rosa damascena) e petitgrain mandarina (Citrus deliciosa) / Antimicrobial action of white sucupira essential oils (Pterodon emarginatus); peach leaves (Prunus persica); juniper berries (Juniperus communis); pink apricot (Rosa damascena) and petitgrain mandarin (Citrus deliciosa)
Fonte: Hig. aliment;32(280/281):69-73, 30/06/2018. tab, ilus.
Idioma: pt.
Resumo: Os óleos essenciais são complexos naturais formados por cerca de 20 ­ 60 componentes em distintas concentrações, sendo caracterizados por dois ou três componentes presentes em maiores concentrações (20 ­ 70%) quando comparados aos demais. A atividade antibacteriana de muitas plantas deve-se aos compostos sintetizados no metabolismo secundário. Tais produtos são conhecidos por suas substâncias ativas. Este trabalho apresentou como objetivo verificar a ação antibacteriana dos óleos essenciais: bagas de junípero (Junipeus communis), folhas de pêssego (Prunus pérsica), petitgrain mandarina (Citrus deliciosa), rosa de damasco 10% (Rosa damascena) e sucupira branca (Pterodon emarginatus). Os testes foram realizados com os óleos essenciais individualmente e combinados. Os óleos essenciais foram impregnados em discos de papel filtro de 6 mm de diâmetro, próprios para antibiograma, colocados em placas de Petri com meio de cultura apropriado, semeado previamente com os seguintes micro-organismos: Bacillus cereus, Bacillus subtilis, Escherichia coli, Salmonella Typhimurium, Salmonella Enteritidis e Staphylococcus aureus, posteriormente incubadas a 35 °C/ 24 ­ 48 horas. Considerou-se de ação antimicrobiana eficaz aqueles que apresentaram halos iguais ou superiores a 10 mm. Resultados eficazes foram observados para o óleo essencial de folhas de pêssego sobre S. aureus (halo de 60 mm) e S. Typhimurium (halo de 62 mm); óleo essencial de folhas de pêssego e petitgrain mandarina sobre B. subtilis (halo de 62 mm). E. coli foi inibida eficientemente por todos os óleos essenciais testados. A atividade inibitória mais eficaz foi observada para o óleo essencial de folhas de pêssego.

Essential oils are natural complexes formed by 20 to 60 components in varying amounts; being characterized by two or three components present in higher concentrations (20-70%) when compared to the others. The antibacterial activity of many plants is due to the compounds synthesized in the secondary metabolism. Such products are known for their active substances. This research aimed to verify the antibacterial action of essential oils: juniper berries (Junipeus communis), peach leaves (Prunus persica), petitgrain mandarin (Citrus deliciosa), apricot rose 10% (Rosa damascena) and sucupira branca (Pterodon emarginatus). The tests were performed with the essential oils individually and in combination. The essential oils were impregnated into 6 mm diameter filter paper disks, suitable for antibiogram, placed in Petri dishes with appropriate culture medium, previously seeded with the following microorganisms: Bacillus cereus, Bacillus subtilis, Escherichia coli, Salmonella Typhimurium, Salmonella Enteritidis and Staphylococcus aureus, subsequently incubated at 35 °C/24 - 48 hours. Efficient antimicrobial action was considered in those essential oils with halos equal to or greater than 10 mm. Efficient results were observed for the essential oil of peach leaves on S. aureus (60 mm halo) and S. Typhimurium (62 mm halo); for essential oil of peach leaves and petitgrain mandarin on B. subtilis (62 mm halo). E. coli was efficiently inhibited for all essential oils tested. The most effective inhibitory activity observed for the essential oil of peach leaves.
Descritores: Óleos Voláteis
Testes de Sensibilidade Microbiana
Escherichia coli
Prunus persica
Anti-Infecciosos
-Juniperus
Rosa damascena
Fitoterapia
Responsável: BR526.1 - Biblioteca de Saúde Pública


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Id: lil-657666
Autor: Diez-de-Medina, Sergio; Silva, Herman.
Título: A molecular marker approach using intron flanking EST-PCR to map candidate genes in peach (Prunus persica)
Fonte: Electron. j. biotechnol;15(5):7-7, Sept. 2012. ilus, tab.
Idioma: en.
Projeto: Consorcio BIOFRUTALES S.A.; . Millennium Nucleus in Plant Cell Biotechnology; . CONICYT.
Resumo: In Peach (Prunus persica) several physiological changes, such as woolliness, triggered by chilling injury are involved in major production losses due to cold storage of the fruits during shipping. Additionally, the low level of polymorphisms among peach varieties is an important limitation in the search for new molecular markers that could be associated with economically important traits. Therefore, a functional approach was employed to associate candidate genes with an informative marker in peach. The data was obtained from the results of an in silico analysis of four different cold peach treatments. Thirty two candidate genes were selected that were aligned against Arabidopsis thaliana genomic sequences to design intron-flanking EST-PCR markers. These markers were used to position the candidate genes on the Prunus genetic reference map. In the physiological response to chilling injury, cell wall integrity, carbohydrate metabolism and stress response pathways could be involved, therefore candidate genes associated by Gene Ontology annotation to these pathways were included in the analysis. The designed markers were positioned to the Texas X Earlygold (TxE) genetic reference map through selective mapping methodology (Bin mapping). 72 percent of these new markers showed polymorphism in the TxE Binset population and 31 percent of them were successfully mapped to a genetic position on the Prunus reference map. The bioinformatic methodology used in this work includes a first approach in search for functional molecular markers associated to differentially expressed genes under certain physiological condition which in addition to the Bin mapping approach allows addressing a genetically anchored position to these new markers.
Descritores: Marcadores Genéticos
Prunus persica/genética
-Mapeamento Cromossômico
Estudos de Associação Genética
Reação em Cadeia da Polimerase
Rosaceae/genética
Responsável: CL1.1 - Biblioteca Central



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