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Pesquisa : B01.650.940.800.575.912.250.987 [Categoria DeCS]
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Id: biblio-889434
Autor: Oliveira Filho, Claudio Costa; Kampke, Edgar Hell; Vargas, Thais Souza; Salustriano, Nathacha Andrade; Scherer, Rodrigo; Fronza, Marcio; Campagnaro, Bianca Prandi.
Título: In vitro cytotoxic activity of five commercial samples of Tribulus terrestris Linn in Espírito Santo (Brazil)
Fonte: Braz. J. Pharm. Sci. (Online);53(4):e00262, 2017. tab, graf.
Idioma: en.
Resumo: ABSTRACT The objective was to investigate the total saponin and protodioscin concentrations and the cytotoxicity in vitro, of five samples of the plant Tribulus terrestris, commercially available in the metropolitan region of Vitória - Espirito Santo, Brazil, and to compare them with the aqueous extract of the plant. The chromatographic profile and quantification of protodioscin in commercial samples and plant extract were evaluated by LC-MS/MS. The percentage of total saponins were determined by the colorimetric method. Extracts and protodioscin cytotoxicity were analyzed by the MTT assay in three cell lineages: fibroblasts (L929), ovarian cancer (Ovcar3) and murine hepatoma (Hepa1c1c7). All extracts displayed high levels of total saponins (207.2 to 780.3 mg g-1 of dry extract). The chromatographic profile revealed a wide diversity of compounds, and the saponin protodioscin was detected in only two extracts. One extract displayed high cytotoxicity, with IC50 values of 157.0, 38.2 and 7.4 µg mL-1 for the Ovcar3, Hepa1c1c7 and L929 cell lines, respectively. The other extracts displayed cytotoxic effects only at concentrations equal to or greater than 125.0 µg mL-1. Surprisingly, the most cytotoxic extract displayed the highest protodioscin concentration. Therefore, it is suggested that these products be marketed with caution, and followed-up by a certified healthcare professional.
Descritores: Saponinas/análise
Extratos Vegetais/análise
Zygophyllaceae/classificação
-Plantas Medicinais/metabolismo
Tipo de Publ: Técnicas In Vitro
Responsável: BR40.1 - DBD - Divisão de Biblioteca e Documentacão do Conjunto das Químicas


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Id: lil-634507
Autor: Toro, M.E.; Oro, N.P.; Vega, A.D.; Maturano, Y.P.; Nally, M.C.; Fernández, E.; Pucheta, E.; Vázquez, F..
Título: Diversidad de levaduras en canopias y suelos asociados con Bulnesia retama y Larrea divaricata / Yeast diversity in Bulnesia retama and Larrea divaricata canopies and associated soils.
Fonte: Rev. argent. microbiol;37(4):209-213, oct.-dic. 2005. tab.
Idioma: es.
Resumo: Los ambientes áridos están dominados por vegetación arbustiva, con acumulación de nutrientes bajo la canopia de los arbustos y con suelos relativamente infértiles en los interparches. Los distintos componentes de los vegetales constituyen uno de los hábitat más comunes para las levaduras. Existen numerosos antecedentes acerca de investigaciones sobre levaduras cuyo hábitat lo constituyen árboles y arbustos, sin embargo no existen referencias sobre levaduras asociadas a las Zigofiláceas, una familia de matorrales preponderantes en la Provincia de Monte argentino. El objetivo de este trabajo fue conocer la biodiversidad de levaduras en suelos y canopias asociados con Bulnesia retama y Larrea divaricata, en los Médanos Grandes de Caucete, San Juan, Argentina. Sobre un total de 87 aislamientos de levaduras identificados, se observó una mayor diversidad taxonómica en las asociadas tanto al suelo como a la parte aérea de B. retama, respecto de L. divaricata. A partir de la canopia de B. retama y su suelo asociado se aislaron 9 y 10 especies de levaduras respectivamente, mientras que de la parte aérea y suelo de L. divaricata 4 y 3. Los géneros identificados fueron: Candida, Debaryomyces, Dekkera, Saccharomyces, Torulaspora, Sporidiobolus y Pichia. En total se encontraron 14 especies en todos los microambientes.

Bush like vegetation dominates arid environments, and there is nutrients accumulation under shrub canopies and relatively unfertile soils between vegetal patches areas. Plants are one of the most common habitats for yeasts. There are many reports about yeasts inhabiting different plant components. Nevertheless, there are no reports about yeasts associated with Zigophyllaceae, an important shrub family of the Argentinean Province of Monte. The objective of this work was to analyzed yeast biodiversity of Bulnesia retama and Larrea divaricata canopies and associated soils, at Médanos Grandes of Caucete, San Juan, Argentina. Eighty seven (87) isolated yeasts were identified. From B. retama canopy and associated soil was observed a larger taxonomical diversity respect to L. divaricata. Nine (9) and ten (10) species were isolated from canopy and associated soil of B. retama, respectively. From L. divaricata canopy were 4 species and 3 species from its associated soil isolated. Identified genera were: Candida, Debaryomyces, Dekkera, Saccharomyces, Torulaspora, Sporidiobolus and Pichia. Fourteen (14) species were found at all microenvironments.
Descritores: Larrea/microbiologia
Microbiologia do Solo
Leveduras/isolamento & purificação
Zygophyllaceae/microbiologia
-Argentina
Biodiversidade
Especificidade da Espécie
Responsável: AR1.2 - Instituto de Investigaciónes Epidemiológicas



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