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Pesquisa : B02.200.825.800.800 [Categoria DeCS]
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Id: biblio-839346
Autor: Hong, Sung-Jun; Park, Chang Eon; Park, Gun-Seok; Kim, Min-Chul; Jung, Byung Kwon; Shin, Jae-Ho.
Título: Draft genome sequence of sulfur-reducing archaeon Thermococcus thioreducens DSM 14981T
Fonte: Braz. j. microbiol;48(1):3-4, Jan.-Mar. 2017.
Idioma: en.
Projeto: Ministry of Education.
Resumo: Abstract Thermococcus thioreducens DSM 14981T, a sulfur-reducing archaeon, was isolated from the rainbow hydrothermal vent site on the Mid-Atlantic Ridge. Herein, we report the draft genome sequence of T. thioreducens DSM 14981T; we obtained 41 contigs with a genome size of 2,052,483 bp and G + C content of 53.5%. This genome sequence will not only help understand how the archaeon adapts to the deep-sea hydrothermal environment but also aid the development of enzymes that are highly stable under extreme conditions for industrial applications.
Descritores: Enxofre/metabolismo
Thermococcus/genética
Thermococcus/metabolismo
Genoma Arqueal
Genômica
Sequenciamento de Nucleotídeos em Larga Escala
-Análise de Sequência de DNA
Biologia Computacional/métodos
Genômica/métodos
Anotação de Sequência Molecular
Responsável: BR1.1 - BIREME


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Id: biblio-1021034
Autor: Mihálik, Daniel; Gubisová, Marcela; Kraic, Ján; Hudcovicová, Martina; Havrlentová, Michaela; Moravcíková, Jana; Glasa, Miroslav; Matusíková, Ildikó.
Título: Introduction of a synthetic Thermococcus-derived α-amlyase gene into barley genome for increased enzyme thermostability in grains
Fonte: Electron. j. biotechnol;30:1-5, nov. 2017. ilus, tab, graf.
Idioma: en.
Projeto: Slovak Research and Development Agency.
Resumo: Background: The enzymes utilized in the process of beer production are generally sensitive to higher temperatures. About 60% of them are deactivated in drying the malt that limits the utilization of starting material in the fermentation process. Gene transfer from thermophilic bacteria is a promising tool for producing barley grains harboring thermotolerant enzymes. Results: Gene for α-amylase from hydrothermal Thermococcus, optimally active at 75­85°C and pH between 5.0 and 5.5, was adapted in silico to barley codon usage. The corresponding sequence was put under control of the endosperm-specific promoter 1Dx5 and after synthesis and cloning transferred into barley by biolistics. In addition to model cultivar Golden Promise we transformed three Slovak barley cultivars Pribina, Levan and Nitran, and transgenic plants were obtained. Expression of the ~50 kDa active recombinant enzyme in grains of cvs. Pribina and Nitran resulted in retaining up to 9.39% of enzyme activity upon heating to 75°C, which is more than 4 times higher compared to non-transgenic controls. In the model cv. Golden Promise the grain α-amylase activity upon heating was above 9% either, however, the effects of the introduced enzyme were less pronounced (only 1.22 fold difference compared with non-transgenic barley). Conclusions: Expression of the synthetic gene in barley enhanced the residual α-amylase activity in grains at high temperatures.
Descritores: Sementes/enzimologia
Hordeum/enzimologia
Thermococcus/metabolismo
alfa-Amilases/metabolismo
-Sementes/genética
Sementes/microbiologia
Transformação Genética
Hordeum/genética
Hordeum/microbiologia
Cerveja
Estabilidade Enzimática
Plantas Geneticamente Modificadas/enzimologia
Clonagem Molecular
Técnicas de Transferência de Genes
alfa-Amilases/genética
Fermentação
Termotolerância
Temperatura Alta
Concentração de Íons de Hidrogênio
Responsável: CL1.1 - Biblioteca Central



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