Base de dados : LILACS
Pesquisa : B03 [Categoria DeCS]
Referências encontradas : 1225 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 123 ir para página                         

  1 / 1225 LILACS  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-951818
Autor: Boujelben, Ines; Gdoura, Radhouane; Hammami, Adnane.
Título: A broad-range PCR technique for the diagnosis of infective endocarditis
Fonte: Braz. j. microbiol;49(3):534-543, July-Sept. 2018. tab, graf.
Idioma: en.
Resumo: Abstract Infective endocarditis (IE) remains a severe and potentially fatal disease demanding sophisticated diagnostic strategies for detection of the causative microorganisms. The aim of the present study was to develop a broad-range 16S ribosomal RNA gene polymerase chain reaction in the routine diagnostic of IE for the early diagnosis of fatal disease. A broad-range PCR technique was selected and evaluated in terms of its efficiency in the diagnosis of endocarditis using 19 heart valves from patients undergoing cardiovascular surgeries at the Habib Bourguiba Hospital of Sfax, Tunisia, on the grounds of suspected IE. The results demonstrated the efficiency of this technique particularly in cases involving a limited number of bacteria since it helped to increase detection sensitivity. The technique proved to be efficient, particularly, in the bacteriological diagnosis of IE in contexts involving negative results from conventional culture methods and other contexts involving bacterial species that were not amenable to identification by phenotypic investigations. Indeed, the sequencing of the partial 16S ribosomal RNA gene revealed the presence of Bartonella henselae, Enterobacter sp., and Streptococcus pyogenes in three heart valves with the negative culture. It should be noted that the results obtained from the polymerase chain reaction-sequencing identification applied to the heart valve and the strain isolated from the same tissue were not consistent with the ones found by the conventional microbiological methods in the case of IE caused by Gemella morbillorum. In fact, the results from the molecular identification revealed the presence of Lactobacillus jensenii. Overall, the results have revealed that the proposed method is sensitive, reliable and might open promising opportunities for the early diagnosis of IE.
Descritores: Bactérias/isolamento & purificação
Reação em Cadeia da Polimerase/métodos
Endocardite/microbiologia
Endocardite Bacteriana/microbiologia
-Filogenia
Bactérias/classificação
Bactérias/genética
DNA Bacteriano/genética
RNA Ribossômico 16S/genética
Endocardite/diagnóstico
Endocardite Bacteriana/diagnóstico
Valvas Cardíacas/microbiologia
Meia-Idade
Limites: Seres Humanos
Masculino
Tipo de Publ: Estudos de Avaliação
Responsável: BR1.1 - BIREME


  2 / 1225 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-951816
Autor: Nawong, Chairat; Umsakul, Kamontam; Sermwittayawong, Natthawan.
Título: Rubber gloves biodegradation by a consortium, mixed culture and pure culture isolated from soil samples
Fonte: Braz. j. microbiol;49(3):481-488, July-Sept. 2018. graf.
Idioma: en.
Resumo: Abstract An increasing production of natural rubber (NR) products has led to major challenges in waste management. In this study, the degradation of rubber latex gloves in a mineral salt medium (MSM) using a bacterial consortium, a mixed culture of the selected bacteria and a pure culture were studied. The highest 18% weight loss of the rubber gloves were detected after incubated with the mixed culture. The increased viable cell counts over incubation time indicated that cells used rubber gloves as sole carbon source leading to the degradation of the polymer. The growth behavior of NR-degrading bacteria on the latex gloves surface was investigated using the scanning electron microscope (SEM). The occurrence of the aldehyde groups in the degradation products was observed by Fourier Transform Infrared Spectroscopy analysis. Rhodococcus pyridinivorans strain F5 gave the highest weight loss of rubber gloves among the isolated strain and posses latex clearing protein encoded by lcp gene. The mixed culture of the selected strains showed the potential in degrading rubber within 30 days and is considered to be used efficiently for rubber product degradation. This is the first report to demonstrate a strong ability to degrade rubber by Rhodococcus pyridinivorans.
Descritores: Borracha/metabolismo
Microbiologia do Solo
Rhodococcus/isolamento & purificação
Rhodococcus/metabolismo
Látex/metabolismo
-Bactérias/isolamento & purificação
Bactérias/classificação
Bactérias/genética
Bactérias/metabolismo
Biodegradação Ambiental
Rhodococcus/classificação
Rhodococcus/genética
Luvas Protetoras/microbiologia
Responsável: BR1.1 - BIREME


  3 / 1225 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-951805
Autor: Umadevi, Palaniyandi; Anandaraj, Muthuswamy; Srivastav, Vivek; Benjamin, Sailas.
Título: Trichoderma harzianum MTCC 5179 impacts the population and functional dynamics of microbial community in the rhizosphere of black pepper (Piper nigrum L )
Fonte: Braz. j. microbiol;49(3):463-470, July-Sept. 2018. tab, graf.
Idioma: en.
Resumo: Abstract Employing Illumina Hiseq whole genome metagenome sequencing approach, we studied the impact of Trichoderma harzianum on altering the microbial community and its functional dynamics in the rhizhosphere soil of black pepper (Piper nigrum L.). The metagenomic datasets from the rhizosphere with (treatment) and without (control) T. harzianum inoculation were annotated using dual approach, i.e., stand alone and MG-RAST. The probiotic application of T. harzianum in the rhizhosphere soil of black pepper impacted the population dynamics of rhizosphere bacteria, archae, eukaryote as reflected through the selective recruitment of bacteria [Acidobacteriaceae bacterium (p = 1.24e-12), Candidatus koribacter versatilis (p = 2.66e-10)] and fungi [(Fusarium oxysporum (p = 0.013), Talaromyces stipitatus (p = 0.219) and Pestalotiopsis fici (p = 0.443)] in terms of abundance in population and bacterial chemotaxis (p = 0.012), iron metabolism (p = 2.97e-5) with the reduction in abundance for pathogenicity islands (p = 7.30e-3), phages and prophages (p = 7.30e-3) with regard to functional abundance. Interestingly, it was found that the enriched functional metagenomic signatures on phytoremediation such as benzoate transport and degradation (p = 2.34e-4), and degradation of heterocyclic aromatic compounds (p = 3.59e-13) in the treatment influenced the rhizosphere micro ecosystem favoring growth and health of pepper plant. The population dynamics and functional richness of rhizosphere ecosystem in black pepper influenced by the treatment with T. harzianum provides the ecological importance of T. harzianum in the cultivation of black pepper.
Descritores: Microbiologia do Solo
Bactérias/crescimento & desenvolvimento
Trichoderma/crescimento & desenvolvimento
Vírus/crescimento & desenvolvimento
Piper nigrum/microbiologia
Biodiversidade
Fungos/crescimento & desenvolvimento
-Bactérias/isolamento & purificação
Bactérias/classificação
Bactérias/genética
Trichoderma/isolamento & purificação
Trichoderma/genética
Vírus/isolamento & purificação
Vírus/classificação
Vírus/genética
Ecossistema
Piper nigrum/crescimento & desenvolvimento
Rizosfera
Fungos/isolamento & purificação
Fungos/classificação
Fungos/genética
Responsável: BR1.1 - BIREME


  4 / 1225 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-951803
Autor: Fernandes, Camila Cesário; Kishi, Luciano Takeshi; Lopes, Erica Mendes; Omori, Wellington Pine; Souza, Jackson Antonio Marcondes de; Alves, Lucia Maria Carareto; Lemos, Eliana Gertrudes de Macedo.
Título: Bacterial communities in mining soils and surrounding areas under regeneration process in a former ore mine
Fonte: Braz. j. microbiol;49(3):489-502, July-Sept. 2018. tab, graf.
Idioma: en.
Projeto: PITE - FAPESP/FAPEMIG/FAPESPA/VALE S.A.; . FAPESP.
Resumo: Abstract Human activities on the Earth's surface change the landscape of natural ecosystems. Mining practices are one of the most severe human activities, drastically altering the chemical, physical and biological properties of the soil environment. Bacterial communities in soil play an important role in the maintenance of ecological relationships. This work shows bacterial diversity, metabolic repertoire and physiological behavior in five ecosystems samples with different levels of impact. These ecosystems belong to a historical area in Iron Quadrangle, Minas Gerais, Brazil, which suffered mining activities until its total depletion without recovery since today. The results revealed Proteobacteria as the most predominant phylum followed by Acidobacteria, Verrucomicrobia, Planctomycetes, and Bacteroidetes. Soils that have not undergone anthropological actions exhibit an increase ability to degrade carbon sources. The richest soil with the high diversity was found in ecosystems that have suffered anthropogenic action. Our study shows profile of diversity inferring metabolic profile, which may elucidate the mechanisms underlying changes in community structure in situ mining sites in Brazil. Our data comes from contributing to know the bacterial diversity, relationship between these bacteria and can explore strategies for natural bioremediation in mining areas or adjacent areas under regeneration process in iron mining areas.
Descritores: Microbiologia do Solo
Bactérias/isolamento & purificação
Biodiversidade
-Filogenia
Bactérias/classificação
Bactérias/genética
Bactérias/metabolismo
Brasil
Ecossistema
Mineração
Responsável: BR1.1 - BIREME


  5 / 1225 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-951796
Autor: Wang, Su; Han, Li-Zhong; Ni, Yu-Xing; Zhang, Yi-Bo; Wang, Qun; Shi, Da-Ke; Li, Wen-Hui; Wang, Yi-Chen; Mi, Chen-Rong.
Título: Changes in antimicrobial susceptibility of commonly clinically significant isolates before and after the interventions on surgical prophylactic antibiotics (SPAs) in Shanghai
Fonte: Braz. j. microbiol;49(3):552-558, July-Sept. 2018. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China.
Resumo: Abstract Surveillances and interventions on antibiotics use have been suggested to improve serious drug-resistance worldwide. Since 2007, our hospital have proposed many measures for regulating surgical prophylactic antibiotics (carbapenems, third gen. cephalosporins, vancomycin, etc.) prescribing practices, like formulary restriction or replacement for surgical prophylactic antibiotics and timely feedback. To assess the impacts on drug-resistance after interventions, we enrolled infected patients in 2006 (pre-intervention period) and 2014 (post-intervention period) in a tertiary hospital in Shanghai. Proportions of targeted pathogens were analyzed: methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Enterococcus spp. (VRE), imipenem-resistant Escherichia coli (IREC), imipenem-resistant Klebsiella pneumoniae (IRKP), imipenem-resistant Acinetobacter baumannii (IRAB) and imipenem-resistant Pseudomonas aeruginosa (IRPA) isolates. Rates of them were estimated and compared between Surgical Department, ICU and Internal Department during two periods. The total proportions of targeted isolates in Surgical Department (62.44%, 2006; 64.09%, 2014) were more than those in ICU (46.13%, 2006; 50.99%, 2014) and in Internal Department (44.54%, 2006; 51.20%, 2014). Only MRSA has decreased significantly (80.48%, 2006; 55.97%, 2014) (p < 0.0001). The percentages of VRE and IREC in 3 departments were all <15%, and the slightest change were also both observed in Surgical Department (VRE: 0.76%, 2006; 2.03%, 2014) (IREC: 2.69%, 2006; 2.63%, 2014). The interventions on surgical prophylactic antibiotics can be effective for improving resistance; antimicrobial stewardship must be combined with infection control practices.
Descritores: Complicações Pós-Operatórias/microbiologia
Bactérias/efeitos dos fármacos
Infecções Bacterianas/microbiologia
Infecção Hospitalar/microbiologia
Antibacterianos/administração & dosagem
-Complicações Pós-Operatórias/prevenção & controle
Bactérias/isolamento & purificação
Bactérias/crescimento & desenvolvimento
Infecções Bacterianas/prevenção & controle
Cuidados Pré-Operatórios
Resistência a Medicamentos
Testes de Sensibilidade Microbiana
China
Infecção Hospitalar/prevenção & controle
Antibioticoprofilaxia
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


  6 / 1225 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-951793
Autor: Wu, Xiukun; Zhang, Gaosen; Zhang, Wei; Liu, Guangxiu; Chen, Tuo; Wang, Yun; Long, Haozhi; Tai, Xisheng; Zhang, Baogui; Li, Zhongqin.
Título: Variations in culturable bacterial communities and biochemical properties in the foreland of the retreating Tianshan No. 1 glacier
Fonte: Braz. j. microbiol;49(3):443-451, July-Sept. 2018. tab, graf.
Idioma: en.
Projeto: Natural Science Foundation of China; . National Basic Research Program (973) of China; . China Postdoctoral Science Fund.
Resumo: Abstract As a glacier retreats, barren areas are exposed, and these barren areas are ideal sites to study microbial succession. In this study, we characterized the soil culturable bacterial communities and biochemical parameters of early successional soils from a receding glacier in the Tianshan Mountains. The total number of culturable bacteria ranged from 2.19 × 105 to 1.30 × 106 CFU g-1 dw and from 9.33 × 105 to 2.53 × 106 CFU g-1 dw at 4 °C and 25 °C, respectively. The number of culturable bacteria in the soil increased at 25 °C but decreased at 4 °C along the chronosequence. The total organic carbon, total nitrogen content, and enzymatic activity were relatively low in the glacier foreland. The number of culturable bacteria isolated at 25 °C was significantly positively correlated with the TOC and TN as well as the soil urease, protease, polyphenoloxidase, sucrase, catalase, and dehydrogenase activities. We obtained 358 isolates from the glacier foreland soils that clustered into 35 groups using amplified ribosomal DNA restriction analysis. These groups are affiliated with 20 genera that belong to six taxa, namely, Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Actinobacteria, Bacteroides, and Deinococcus-Thermus, with a predominance of members of Actinobacteria and Proteobacteria in all of the samples. A redundancy analysis showed that the bacterial succession was divided into three periods, an early stage (10a), a middle stage (25-74a), and a late stage (100-130a), with the total number of culturable bacteria mainly being affected by the soil enzymatic activity, suggesting that the microbial succession correlated with the soil age along the foreland.
Descritores: Bactérias/isolamento & purificação
Camada de Gelo/microbiologia
Camada de Gelo/química
-Filogenia
Solo/química
Microbiologia do Solo
Bactérias/classificação
Bactérias/crescimento & desenvolvimento
Bactérias/genética
DNA Bacteriano/genética
DNA Ribossômico/genética
RNA Ribossômico 16S/genética
China
Análise de Sequência de DNA
Nitrogênio/análise
Nitrogênio/metabolismo
Responsável: BR1.1 - BIREME


  7 / 1225 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-974330
Autor: Moura, Fábio Gomes; Graças, Diego Assis das; Santos, Agenor Valadares; Silva, Artur Luiz da Costa da; Rogez, Hervé.
Título: Dynamics and diversity of the bacterial community during the spontaneous decay of açai (Euterpe oleracea) fruits
Fonte: Braz. j. microbiol;49(supl.1):25-33, 2018. tab, graf.
Idioma: en.
Resumo: Abstract The biodiversity and evolution of the microbial community in açai fruits (AF) between three geographical origins and two spontaneous decay conditions were examined by applying culture-independent methods. Culture-independent methods based on 16S rRNA from fifteen samples revealed that Proteobacteria, Firmicutes, Actinobacteria, Bacteroidetes and Acidobacteria were the most abundant phyla. At the genus level, Massilia (taxon with more than 50% of the sequences remaining constant during the 30 h of decay), Pantoea, Naxibacter, Enterobacter, Raoultella and Klebsiella were identified, forming the carposphere bacterial microbiota of AF. AF is fibre-rich and Massilia bacteria could find a large quantity of substrate for its growth through cellulase production. Beta diversity showed that the quality parameters of AF (pH, soluble solids, titratable acidity and lipids) and elemental analysis (C, N, H and C/N ratio) were unable to drive microbial patterns in AF. This research offers new insight into the indigenous bacterial community composition on AF as a function of spontaneous postharvest decay.
Descritores: Bactérias/isolamento & purificação
Euterpe/química
Frutas/microbiologia
-Filogenia
Bactérias/classificação
Bactérias/genética
Bactérias/metabolismo
Biodiversidade
Sequenciamento de Nucleotídeos em Larga Escala
Microbiota
Euterpe/microbiologia
Frutas/química
Responsável: BR1.1 - BIREME


  8 / 1225 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-974327
Autor: Souza, Juliana Leitzke Santos de; Guimarães, Victoria Burmann da Silva; Campos, Angela Diniz; Lund, Rafael Guerra.
Título: Antimicrobial potential of pyroligneous extracts - a systematic review and technological prospecting
Fonte: Braz. j. microbiol;49(supl.1):128-139, 2018. tab, graf.
Idioma: en.
Projeto: CAPES; . FAPERGS.
Resumo: Abstract Pyroligneous extract is applied in diverse areas as an antioxidant, an antimicrobial, and an anti-inflammatory agent. The discovery of new cost-effective antimicrobial agents of natural origin remains a challenge for the scientific community. This study aimed to conduct a systematic review and a technological forecasting of the existent evidence regarding the use of pyroligneous extract as a potential antimicrobial agent. Studies were identified through an investigation of various electronic databases: PubMed, SciFinder, Web of Science, Scopus, Scielo, Google scholar, and ProQuest. Patents were searched through INPI, Google patents, Espacenet, Patents online, USPTO, and WIPO. The literature on antimicrobial activity of pyroligneous extract are limited given the short duration of studies and variability in study design, use of pyroligneous preparations, and reports on results. However, evidence suggests the potential of pyroligneous extract as a natural antimicrobial agent. The most studied activity was the role of PE as a food preservative. However, pyroligneous extracts are also effective against pathogenic bacteria in the oral microflora and treatment of candidal infections. Further research is needed using standardized preparations of pyroligneous extracts to determine their long-term effectiveness and ability as antimicrobial agents.
Descritores: Madeira/química
Extratos Vegetais/farmacologia
Conservantes de Alimentos/farmacologia
Anti-Infecciosos/farmacologia
-Bactérias/efeitos dos fármacos
Extratos Vegetais/química
Conservantes de Alimentos/química
Fungos/efeitos dos fármacos
Anti-Infecciosos/química
Limites: Seres Humanos
Animais
Responsável: BR1.1 - BIREME


  9 / 1225 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-974307
Autor: Zhang, Nan; Peng, Huijuan; Li, Yong; Yang, Wenxiu; Zou, Yuneng; Duan, Huiguo.
Título: Ammonia determines transcriptional profile of microorganisms in anaerobic digestion
Fonte: Braz. j. microbiol;49(4):770-776, Oct.-Dec. 2018. tab, graf.
Idioma: en.
Projeto: Key Project of Education Department of Sichuan Province; . Major Cultivation Project of Education Department of Sichuan Province.
Resumo: ABSTRACT Anaerobic digestion is important for the management of livestock manure with high ammonia level. Although ammonia effects on anaerobic digestion have been comprehensively studied, the molecular mechanism underlying ammonia inhibition still remains elusive. In this study, based on metatranscriptomic analysis, the transcriptional profile of microbial community in anaerobic digestion under low (1500 mg L-1) and high NH4 + (5000 mg L-1) concentrations, respectively, were revealed. The results showed that high NH4 + concentrations significantly inhibited methane production but facilitated the accumulations of volatile fatty acids. The expression of methanogenic pathway was significantly inhibited by high NH4 + concentration but most of the other pathways were not significantly affected. Furthermore, the expressions of methanogenic genes which encode acetyl-CoA decarbonylase and methyl-coenzyme M reductase were significantly inhibited by high NH4 + concentration. The inhibition of the co-expressions of the genes which encode acetyl-CoA decarbonylase was observed. Some genes involved in the pathways of aminoacyl-tRNA biosynthesis and ribosome were highly expressed under high NH4 + concentration. Consequently, the ammonia inhibition on anaerobic digestion mainly focused on methanogenic process by suppressing the expressions of genes which encode acetyl-CoA decarbonylase and methyl-coenzyme M reductase. This study improved the accuracy and depth of understanding ammonia inhibition on anaerobic digestion.
Descritores: Bactérias/genética
Bactérias/metabolismo
Amônia/metabolismo
-Bactérias/isolamento & purificação
Bactérias/classificação
Transcrição Genética
Reatores Biológicos/microbiologia
Ácidos Graxos Voláteis/metabolismo
Microbiota
Anaerobiose
Metano/metabolismo
Responsável: BR1.1 - BIREME


  10 / 1225 LILACS  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-974306
Autor: Dealtry, Simone; Ghizelini, Angela Michelato; Mendonça-Hagler, Leda C. S; Chaloub, Ricardo Moreira; Reinert, Fernanda; Campos, Tácio M. P. de; Gomes, Newton C. M; Smalla, Kornelia.
Título: Petroleum contamination and bioaugmentation in bacterial rhizosphere communities from Avicennia schaueriana
Fonte: Braz. j. microbiol;49(4):757-769, Oct.-Dec. 2018. tab, graf.
Idioma: en.
Projeto: Deutsche Forschungsgemeinschaft; . FAPERJ-Brazil.
Resumo: ABSTRACT Anthropogenic activity, such as accidental oil spills, are typical sources of urban mangrove pollution that may affect mangrove bacterial communities as well as their mobile genetic elements. To evaluate remediation strategies, we followed over the time the effects of a petroleum hydrocarbon degrading consortium inoculated on mangrove tree Avicennia schaueriana against artificial petroleum contamination in a phytoremediation greenhouse experiment. Interestingly, despite plant protection due to the inoculation, denaturing gradient gel electrophoresis of the bacterial 16S rRNA gene fragments amplified from the total community DNA indicated that the different treatments did not significantly affect the bacterial community composition. However, while the bacterial community was rather stable, pronounced shifts were observed in the abundance of bacteria carrying plasmids. A PCR-Southern blot hybridization analysis indicated an increase in the abundance of IncP-9 catabolic plasmids. Denaturing gradient gel electrophoresis of naphthalene dioxygenase (ndo) genes amplified from cDNA (RNA) indicated the dominance of a specific ndo gene in the inoculated petroleum amendment treatment. The petroleum hydrocarbon degrading consortium characterization indicated the prevalence of bacteria assigned to Pseudomonas spp., Comamonas spp. and Ochrobactrum spp. IncP-9 plasmids were detected for the first time in Comamonas sp. and Ochrobactrum spp., which is a novelty of this study.
Descritores: Bactérias/isolamento & purificação
Bactérias/metabolismo
Avicennia/microbiologia
Hidrocarbonetos/metabolismo
-Plasmídeos/genética
Plasmídeos/metabolismo
Poluentes do Solo/análise
Poluentes do Solo/metabolismo
Bactérias/classificação
Bactérias/genética
Biodegradação Ambiental
DNA Bacteriano/genética
Petróleo/análise
RNA Ribossômico 16S/genética
Poluição por Petróleo/análise
Avicennia/metabolismo
Rizosfera
Responsável: BR1.1 - BIREME



página 1 de 123 ir para página                         
   


Refinar a pesquisa
  Base de dados : Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde