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Id: lil-622819
Autor: Cihan, Arzu Coleri; Tekin, Nilgun; Ozcan, Birgul; Cokmus, Cumhur.
Título: The genetic diversity of genus Bacillus and the related genera revealed by 16S rRNA gene sequences and ardra analyses isolated from geothermal regions of turkey
Fonte: Braz. j. microbiol;43(1):309-324, Jan.-Mar. 2012. ilus, tab.
Idioma: en.
Resumo: Previously isolated 115 endospore-forming bacilli were basically grouped according to their temperature requirements for growth: the thermophiles (74%), the facultative thermophiles (14%) and the mesophiles (12%). These isolates were taken into 16S rRNA gene sequence analyses, and they were clustered among the 7 genera: Anoxybacillus, Aeribacillus, Bacillus, Brevibacillus, Geobacillus, Paenibacillus, and Thermoactinomycetes. Of these bacilli, only the thirty two isolates belonging to genera Bacillus (16), Brevibacillus (13), Paenibacillus (1) and Thermoactinomycetes (2) were selected and presented in this paper. The comparative sequence analyses revealed that the similarity values were ranged as 91.4-100 %, 91.8- 99.2 %, 92.6- 99.8 % and 90.7 - 99.8 % between the isolates and the related type strains from these four genera, respectively. Twenty nine of them were found to be related with the validly published type strains. The most abundant species was B. thermoruber with 9 isolates followed by B. pumilus (6), B. lichenformis (3), B. subtilis (3), B. agri (3), B. smithii (2), T. vulgaris (2) and finally P. barengoltzii (1). In addition, isolates of A391a, B51a and D295 were proposed as novel species as their 16S rRNA gene sequences displayed similarities ¡Ü 97% to their closely related type strains. The AluI-, HaeIII- and TaqI-ARDRA results were in congruence with the 16S rRNA gene sequence analyses. The ARDRA results allowed us to differentiate these isolates, and their discriminative restriction fragments were able to be determined. Some of their phenotypic characters and their amylase, chitinase and protease production were also studied and biotechnologically valuable enzyme producing isolates were introduced in order to use in further studies.
Descritores: Bacilos Gram-Positivos Formadores de Endosporo/genética
Bacilos Gram-Positivos Formadores de Endosporo/isolamento & purificação
Enzimas Reparadoras do DNA
RNA
-Microbiologia Ambiental
Tipo de Publ: Estudo Comparativo
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica


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Id: lil-523737
Autor: Sequeira, Bianca Jorge; Vital, Marcos Jose Salgado; Pohlit, Adrian Martin; Pararols, Isabel Costa; Caúper, Glaucia Socorro Barros.
Título: Antibacterial and antifungal activity of extracts and exudates of the Amazonian medicinal tree Himatanthus articulatus (Vahl) Woodson (common name: sucuba)
Fonte: Mem. Inst. Oswaldo Cruz;104(4):659-661, July 2009. tab.
Idioma: en.
Projeto: CNPq.
Resumo: Himatanthus articulatus (Vahl) Woodson is a tree found in the northern Amazon savannahs (common name: sucuba) that is used in local Amerindian medicine. Leaf, bark and branch wood methanol extracts, sequentially obtained hexane, ethyl acetate and methanol extracts and latex were evaluated for antifungal and antibacterial activities against American Type Culture Collection (ATCC) and local clinical strains using the disc diffusion method. Methanol extracts and latex inhibited Candida albicans, leaf methanol extracts inhibited Staphylococcus aureus and Bacillus subtilis and bark methanol extracts inhibited B. subtilis. Active extracts inhibited the ATCC and clinical strains. Polar antifungal and antibacterial principles in latex and extracts are thought to be responsible for the inhibition.
Descritores: Antibacterianos/farmacologia
Antifúngicos/farmacologia
Apocynaceae/química
Candida albicans/efeitos dos fármacos
Escherichia coli/efeitos dos fármacos
Bacilos Gram-Positivos Formadores de Endosporo/efeitos dos fármacos
-Antibacterianos/isolamento & purificação
Antifúngicos/isolamento & purificação
Bacillus subtilis/efeitos dos fármacos
Testes de Sensibilidade a Antimicrobianos por Disco-Difusão
Extratos Vegetais/farmacologia
Staphylococcus aureus/efeitos dos fármacos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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