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Pesquisa : B03.300.390.400.158 [Categoria DeCS]
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Id: biblio-889192
Autor: Strahsburger, Erwin; Zapata, Felipe; Pedroso, Inti; Fuentes, Derie; Tapia, Paz; Ponce, Raul; Valdes, Jorge.
Título: Draft genome sequence of Exiguobacterium aurantiacum strain PN47 isolate from saline ponds, known as "Salar del Huasco", located in the Altiplano in the North of Chile
Fonte: Braz. j. microbiol;49(1):7-9, Jan.-Mar. 2018.
Idioma: en.
Projeto: Academic Vice Rectory of Arturo Prat University; . International Centers of Excellence.
Resumo: ABSTRACT In this report, we present a draft genome of 2,886,173 bp of an Exiguobacterium aurantiacum strain PN47 isolate from the sediment of a saline pond named "Salar del Huasco" in the Altiplano in the North of Chile. Strain PN47 encodes adaptive characteristics enabling survival in extreme environmental conditions of high heavy metal and salt concentrations and high alkalinity.
Descritores: Bacillaceae/isolamento & purificação
Bacillaceae/genética
Tanques/microbiologia
Genoma Bacteriano
-Filogenia
Bacillaceae/classificação
Bacillaceae/metabolismo
DNA Bacteriano/genética
Sequência de Bases
Cloreto de Sódio/análise
Cloreto de Sódio/metabolismo
Tanques/química
Chile
Metais Pesados/análise
Metais Pesados/metabolismo
Responsável: BR1.1 - BIREME


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Id: lil-749707
Autor: Aanniz, Tarik; Ouadghiri, Mouna; Melloul, Marouane; Swings, Jean; Elfahime, Elmostafa; Ibijbijen, Jamal; Ismaili, Mohamed; Amar, Mohamed.
Título: Thermophilic bacteria in Moroccan hot springs, salt marshes and desert soils
Fonte: Braz. j. microbiol;46(2):443-453, Apr-Jun/2015. tab, graf.
Idioma: en.
Resumo: The diversity of thermophilic bacteria was investigated in four hot springs, three salt marshes and 12 desert sites in Morocco. Two hundred and forty (240) thermophilic bacteria were recovered, identified and characterized. All isolates were Gram positive, rod-shaped, spore forming and halotolerant. Based on BOXA1R-PCR and 16S rRNA gene sequencing, the recovered isolates were dominated by the genus Bacillus (97.5%) represented by B. licheniformis (119), B. aerius (44), B. sonorensis (33), B. subtilis (subsp. spizizenii (2) and subsp. inaquosurum (6)), B. amyloliquefaciens (subsp. amyloliquefaciens (4) and subsp. plantarum (4)), B. tequilensis (3), B. pumilus (3) and Bacillus sp. (19). Only six isolates (2.5%) belonged to the genus Aeribacillus represented by A. pallidus (4) and Aeribacillus sp. (2). In this study, B. aerius and B. tequilensis are described for the first time as thermophilic bacteria. Moreover, 71.25%, 50.41% and 5.41% of total strains exhibited high amylolytic, proteolytic or cellulolytic activity respectively.
Descritores: Bacillaceae/classificação
Bacillaceae/isolamento & purificação
Bacilos Gram-Positivos/classificação
Bacilos Gram-Positivos/isolamento & purificação
Fontes Termais/microbiologia
Microbiologia do Solo
Microbiologia da Água
-Biodiversidade
Bacillaceae/genética
Bacillaceae/efeitos da radiação
Análise por Conglomerados
DNA Bacteriano/química
DNA Bacteriano/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Bacilos Gram-Positivos/genética
Bacilos Gram-Positivos/efeitos da radiação
Dados de Sequência Molecular
Marrocos
Filogenia
/genética
RNA, RIBOSOMAL, ABNORMALITIES, MULTIPLES/genética
Análise de Sequência de DNA
Esporos Bacterianos/citologia
Responsável: BR1.1 - BIREME


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Id: lil-672048
Autor: Berlemont, Renaud; Spee, Olivier; Delsaute, Maud; Lara, Yannick; Schuldes, Jörg; Simon, Carola; Power, Pablo; Daniel, Rolf; Galleni, Moreno.
Título: Novel organic solvent-tolerant esterase isolated by metagenomics: insights into the lipase/esterase classification / Nueva esterasa tolerante a los solventes orgánicos aislada por metagenómica: ideas sobre la clasificación de las esterasas/lipasas
Fonte: Rev. argent. microbiol;45(1):3-12, mar. 2013. graf, tab.
Idioma: en.
Resumo: In order to isolate novel organic solvent-tolerant (OST) lipases, a metagenomic library was built using DNA derived from a temperate forest soil sample. A two-step activity-based screening allowed the isolation of a lipolytic clone active in the presence of organic solvents. Sequencing of the plasmid pRBest recovered from the positive clone revealed the presence of a putative lipase/esterase encoding gene. The deduced amino acid sequence (RBest1) contains the conserved lipolytic enzyme signature and is related to the previously described OST lipase from Lysinibacillus sphaericus 205y, which is the sole studied prokaryotic enzyme belonging to the 4.4 a/ß hydrolase subgroup (abH04.04). Both in vivo and in vitro studies of the substrate specificity of RBest1, using triacylglycerols or nitrophenyl-esters, respectively, revealed that the enzyme is highly specific for butyrate (C4) compounds, behaving as an esterase rather than a lipase. The RBest1 esterase was purified and biochemically characterized. The optimal esterase activity was observed at pH 6.5 and at temperatures ranging from 38 to 45 °C. Enzymatic activity, determined by hydrolysis of p-nitrophenyl esters, was found to be affected by the presence of different miscible and non-miscible organic solvents, and salts. Noteworthy, RBest1 remains significantly active at high ionic strength. These findings suggest that RBest1 possesses the ability of OST enzymes to molecular adaptation in the presence of organic compounds and resistance of halophilic proteins.

Con el fin de aislar nuevas variantes de lipasas tolerantes a solventes organicos (OST), se construyo una libreria metagenomica a partir de ADN obtenido de una muestra de suelo de bosque templado. A traves de un monitoreo en dos etapas, basado en la deteccion de actividades, se aislo un clon con actividad lipolitica en presencia de solventes organicos. La secuenciacion del plasmido pRBest recuperado del clon positivo revelo la presencia de un gen codificante de una hipotetica lipasa/esterasa. La secuencia deducida de amino acidos (RBest1) contiene los motivos conservados de enzimas lipoliticas y esta relacionada con la lipasa OST previamente descrita de Lysinibacillus sphaericus 205y, que es la unica enzima procariota estudiada perteneciente al subgrupo 4.4 de a/ß hidrolasas (abH4.04). Estudios in vivo e in vitro sobre la especificidad de sustratos de RBest1, utilizando triacil-gliceroles o p-nitrofenil-esteres, respectivamente, revelaron que la enzima es altamente especifica para compuestos butiricos (C4), comportandose como una esterasa y no como una lipasa. La esterasa RBest1 fue purificada y caracterizada bioquimicamente. La actividad optima de esterasa fue observada a pH 6,5 y las temperaturas optimas fueron entre 38 y 45 °C. Se establecio que la actividad enzimatica, determinada por hidrolisis de p-nitrofenil esteres, es afectada en presencia de diferentes solventes organicos miscibles y no miscibles, y tambien sales. Notoriamente, RBest1 permanece significativamente activa a elevadas fuerzas ionicas. Estos hallazgos sugieren que RBest1 posee la capacidad de las enzimas OST de la adaptacion molecular en presencia de compuestos organicos, asi como la resistencia de las proteinas halofilas.
Descritores: Esterases/isolamento & purificação
Lipase/isolamento & purificação
Metagenômica
-Sequência de Aminoácidos
Bacillaceae/enzimologia
Proteínas de Bactérias/química
Butiratos/metabolismo
Sequência Conservada
DNA
Esterases/classificação
Alemanha
Concentração de Íons de Hidrogênio
Hidrólise
Lipólise
Lipase/classificação
Dados de Sequência Molecular
Concentração Osmolar
Filogenia
Proteínas Recombinantes/metabolismo
Alinhamento de Sequência
Homologia de Sequência de Aminoácidos
Microbiologia do Solo
Especificidade por Substrato
Sais/farmacologia
Solventes/farmacologia
Temperatura Ambiente
Árvores
Triglicerídeos/metabolismo
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: AR1.2 - Instituto de Investigaciónes Epidemiológicas


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Id: lil-650087
Autor: Cárdenas Castro, Estrella; Rozo Bautista, Álvaro; Lugo Vargas, Ligia.
Título: Mortalidad en larvas de Culex quinquefasciatus y Anopheles albimanus (díptera: culicidae), causada con un producto de Bacillus sphaericus (bacteria: bacillaceae) en presentación granulada en condiciones experimentales / Larval Mortality in Culexquinquefasciatus and Anopheles albimanus (diptera: culicidae), Caused with a Bacillus sphaericus (bacteria: bacillaceae) Granulated Product in Experimental Conditions
Fonte: Rev. med. vet. (Bogota);(23):23-32, jun. 2012. ilus, tab.
Idioma: es.
Resumo: El objetivo de este estudio fue ensayar una serie de concentraciones de un producto granulado preparado con Bacillus sphaericus, sobre larvas de Culex quinquefasciatus y Anopheles albimanusen condiciones de laboratorio. Se utilizaron once concentraciones (20, 40, 60, 80,100, 120, 140, 160, 180, 200 y 500 ppm) sobre larvas de An. albimanus, y ocho concentraciones diez veces menores (2, 4, 6, 8, 10, 12, 14 y 16 ppm) sobre larvas de Cx. quinquefasciatus.Se utilizaron 60 larvas y un control con 20 larvas por concentración. El tiempo de exposición fue de 48 h, a una temperatura de 28 ± 2 ºC. Para estimar las concentraciones letales 50 y 95 se utilizó la prueba Probit. Se encontró una CL95 de B. sphaericus entre 6,45 y 7,28 ppm para larvas de Cx. quinquefasciatus; mientras que en larvas de An. albimanus se observó una CL95entre 450,56 y 466,76 ppm...

The purpose of this study was to assay a series of concentrations of a granulated productprepared with Bacillus sphaericus on Culexquinquefasciatus and Anopheles albimanus larvae inlaboratory conditions. Eleven concentrations (20, 40, 60, 80, 100, 120, 140, 160, 180, 200,500 ppm) were used on An. Albimanus larvae and eight concentrations ten times smaller (2,4, 6, 8, 10, 12, 14, 16 ppm) were used on Cx. quinquefasciatus larvae. Sixty (60) larvae and acontrol with 20 larvae were used per concentration. The time of exposure was of 48 hours ata laboratory temperature of 28 ± 2 ºC. LC50 and LC95 were determined through the Probittests. A LC95 of B. sphaericus was found between 6,45 and 7,28 ppm for Cx. quinquefasciatuslarvae; whereas LC95 was observed between 450.56 and 466.76 ppm in An. albimanus larvae...

O objetivo deste estudo foi testar uma série de concentrações de um produto granulado preparadocom Bacillus sphaericus, sobre larvas de Culex quinquefasciatus e Anopheles albimanusem condições de laboratório. Utilizaram-se onze concentrações (20, 40, 60, 80, 100, 120,140, 160, 180, 200 e 500 ppm) sobre larvas de An. albimanus, e oito concentrações dez vezesmenores (2, 4, 6, 8, 10, 12, 14 e 16 ppm) sobre larvas de Cx. quinquefasciatus. Utilizaram-se60 larvas e um controle com 20 larvas por concentração. O tempo de exposição foi de 48h,a uma temperatura de 28 ± 2 ºC. Para estimar as concentrações letais 50 e 95 utilizou-seo teste Probit. Encontrou-se uma CL95 de B. sphaericus entre 6,45 e 7,28 ppm para larvasde Cx. quinquefasciatus; enquanto que em larvas de An. Albimanus observou-se uma CL95entre 450,56 e 466,76 ppm...
Descritores: Bactérias
Culicidae
Mortalidade
Controle Biológico de Vetores
-Bacillaceae
Limites: Animais
Responsável: CO149 - Facultad de Medicina Veterinária


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Id: lil-640501
Autor: Ebrahimpour, Afshin; Rahman, Raja Noor Zaliha Raja Abd; Kamarudin, Nor Hafizah Ahmad; Basri, Mahiran; Salleh, Abu Bakar.
Título: Lipase production and growth modeling of a novel thermophilic bacterium: aneurinibacillus thermoaerophilus strain AFNA
Fonte: Electron. j. biotechnol;14(4):6-6, July 2011. ilus, tab.
Idioma: en.
Resumo: Aneurinibacillus thermoaerophilus strain AFNA as a novel isolated extracellular thermostable organic solvent tolerant lipase producing bacterium was employed in the present study. The lipase production of strain AFNA and its correlation with bacterial growth was studied via a modeling assessment by response surface methodology (RSM) and artificial neural network (ANN) techniques. The best achieved models were multilayer full feed forward incremental back propagation network and modified cubic response surface model (mRSM) using backward elimination. The highest lipase specific activity (13.1 Umg-1) and bacterial growth (OD600 = 3.0) were obtained at technically similar: growth temperature (53 and 53ºC), inoculum size (2.6 and 3.0 percent), agitation rate (118 and 115 rpm) and initial pH (7.0 and 7.2) but different medium volume (139 and 87 ml) and incubation period (48 and 38 hrs), respectively. In addition, the importance of effective parameters on the bacterial growth and lipase production was studied where pH and inoculum size were the most and the least effective factors, respectively. Significant correlation between lipase production and bacterial growth was observed when Bivariate correlation was employed to analyse the data. As a conclusion, lipase production was the result of a synergistic combination of effective parameters interactions and these parameters were in equilibrium.
Descritores: Bacillaceae
Lipase/biossíntese
Redes Neurais (Computação)
-Modelos Teóricos
Propriedades de Superfície
Temperatura Ambiente
Responsável: CL1.1 - Biblioteca Central


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Id: lil-640499
Autor: Ibrahim, Abdelnasser S. S; El-Tayeb, Mohamed A; Elbadawi, Yahya B; Al-Salamah, Ali A.
Título: Isolation and characterization of novel potent Cr(VI) reducing alkaliphilic amphibacillus sp. ksuCr3 from hypersaline soda lakes
Fonte: Electron. j. biotechnol;14(4):4-4, July 2011. ilus, tab.
Idioma: en.
Projeto: King Saud University. Deanship of Scientific Research.
Resumo: A strain KSUCr3 with extremely high Cr(VI)-reducing ability under alkaline conditions was isolated from hypersaline soda lakes and identified as Amphibacillus sp. on the basis of 16S rRNA gene sequence analysis. The results showed that Amphibacillus sp. strain KSUCr3 was tolerance to very high Cr(VI) concentration (75 mM) in addition to high tolerance to other heavy metals including Ni2+ (100 mM), Mo2+ (75 mM), Co2+ (5 mM), Mn2+ (100 mM), Zn2+ (2 mM), Cu2+ (2 mM) and Pb (75 mM). Strain KSUCr3 was shown to be of a high efficiency in detoxifying chromate, as it could rapidly reduce 5 mM of Cr(VI) to a non detectable level over 24 hrs. In addition, strain KSUCr3 could reduce Cr(VI) efficiently over a wide range of initial Cr(VI) concentrations (1-10 mM) in alkaline medium under aerobic conditions without significant effect on the bacterial growth. Addition of glucose, NaCl and Na2CO3 to the culture medium caused a dramatic increase in Cr(VI)-reduction by Amphibacillus sp. strain KSUCr3. The maximum chromate removal was exhibited in alkaline medium containing 1.5 percent Na2CO3, 0.8 percent glucose, and 1.2 percent NaCl, at incubation temperature of 40ºC and shaking of 100 rpm. Under optimum Cr(VI) reduction conditions, Cr(VI) reduction rate reached 237 uMh¹ which is one of the highest Cr(VI) reduction rate, under alkaline conditions and high salt concentration, compared to other microorganisms that has been reported so far. Furthermore, the presence of other metals, such as Ni2+, Co2+, Cu2+ and Mn2+ slightly stimulated Cr(VI)-reduction ability by the strain KSUCr3.The isolate, Amphibacillus sp. strain KSUCr3, exhibited an ability to repeatedly reduce hexavalent chromium without any amendment of nutrients, suggesting its potential application in continuous bioremediation of Cr(VI). The results also revealed the possible isolation of potent heavy metals resistant bacteria from extreme environment such as hypersaline soda lakes.
Descritores: Bacillaceae
Biodegradação Ambiental
Cromo/metabolismo
Oxirredutases/metabolismo
-Lagos
Metais Pesados
Oxirredutases/isolamento & purificação
Responsável: CL1.1 - Biblioteca Central



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