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Pesquisa : B03.300.390.400.158.218 [Categoria DeCS]
Referências encontradas : 201 [refinar]
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Id: biblio-1146421
Autor: Toledo, Adrieli Gorlin; Souza, Juliete Gomes de Lara de; Silva, Jéssica Patrícia Borges da; Favreto, Wagner Alex Jann; Costa, Willian Ferreira da; Pinto, Fabiana Gisele da Silva.
Título: Chemical composition, antimicrobial and antioxidant activity of the essential oil of leaves of Eugenia involucrata DC / Composição química, atividade antimicrobiana e antioxidante do óleo essencial das folhas de Eugenia involucrata DC
Fonte: Biosci. j. (Online);36(2):568-577, 01-03-2020. tab.
Idioma: en.
Resumo: In the Myrtaceae family, the species Eugenia involucrata DC., popularly known as "cerejeira-do-mato", is traditionally used for the antidiarrheal and digestive action of its leaves. However, no studies were found in the literature regarding its antimicrobial and antioxidant potential. In this context, the objective of the present study was to determine the chemical composition by gas chromatography coupled to mass spectrometry (GC-MS) to evaluate the antimicrobial activity by the broth microdilution technique and the antioxidant activity by the 2,2-diphenyl-1-picryl-hydrazila (DPPH) method of the essential oil of E. involucrataleaves. GC-MS identified 28 compounds, all sesquiterpenes, corresponding to 89.41% of the essential oil. The antimicrobial activity of the essential oil was observed for all Gram-positive bacteria tested (Staplylococcus epidermidis, Enterococcus faecalis,Bacillus subtilis and Staplylococcus aureus) and for yeast Candida albicans. The essential oil presented a reduction capacity of DPPH up to 66.81%, evidencing its antioxidant potential. It is suggested that the antimicrobial and antioxidant action of E. involucrata essential oil is related to the presence of the major compounds, elixene (26.53%), ß-caryophyllene (13.16%), α-copaene (8.41%) and germacrene D (7.17%).

Na família Myrtaceae, a espécieEugenia involucrata DC. popularmente denominada "cerejeira-do-mato" é conhecida tradicionalmente pela ação antidiarreica e digestiva de suas folhas. Contudo, na literatura não foram encontrados trabalhos referentes ao seu potencial antimicrobiano e antioxidante. Neste contexto, o objetivo do presente estudo foi determinar a composição química por cromatografia gasosa acoplada a espectrometria de massas (CG-EM) e avaliar a atividade antimicrobiana pela técnica de microdiluição em caldo e a atividade antioxidante pelo método do 2,2-difenil-1-picril-hidrazila (DPPH) do óleo essencial das folhas de E. involucrata. A CG-EM identificou 28 compostos, todos sesquiterpenos, correspondendo a 89,41% do óleo essencial. A atividade antimicrobiana do óleo essencial foi observada para todas as bactérias Gram-positivas testadas (Staplylococcus epidermidis, Enterococcus faecalis,Bacillus subtilise Staplylococcus aureus) e para a levedura Candida albicans. O óleo essencial apresentou capacidade redutora de radicais DPPH de até 66,81%, evidenciando sua potencialidade antioxidante. Sugere-se que a ação antimicrobiana e antioxidante do óleo essencial de E. involucrata esteja relacionada à presença dos compostos majoritários, elixeno (26,53%), ß-cariofileno (13,16%), -copaeno (8,41%) e germacreno D (7,17%).
Descritores: Myrtaceae
Eugenia
Anti-Infecciosos
Antioxidantes
-Espectrometria de Massas
Bacillus
Bactérias
Produtos Biológicos
Candida albicans
Óleos
Destilação
Cromatografia Gasosa
Enterococcus faecalis
Compostos Orgânicos Voláteis
Responsável: BR396.1 - Biblioteca Central


  2 / 201 LILACS  
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Id: biblio-1146668
Autor: Blanco, Neder Henrique Martinez; Barbosa, Danilo Ferreira Ramirez; Graichen, Felipe André Sganzerla.
Título: Antagonistic microorganisms and nitrogen fertilization in control of tomato southern blight
Fonte: Arq. Inst. Biol;88:e00502019, 2021. ilus, graf.
Idioma: en.
Projeto: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; . Universidade Estadual de Mato Grosso do Sul/FUNDECT.
Resumo: The present study assessed the efficacy of formulated biocontrol agents and nitrogen fertilization on southern blight control. Antagonism test in vitro was performed to assess the inhibitory activity of Bacillus methylotrophicus and Trichoderma asperellum against the growth of Sclerotium rolfsii. Tomato seedlings were transplanted into the substrate added with ammonium nitrate doses and inoculated with the formulated biocontrol agents Ônix (B. methylotrophicus) or Quality (T. asperellum). Subsequently, seedlings were inoculated with S. rolfsii. Plant mortality, shoot and root weight were assessed 11 days after the last inoculation. Agents had effective inhibitory activity against S. rolfsii; thus, they could reduce southern blight severity when combined with ammonium nitrate. However, plant mortality was not reduced by them.(AU)
Descritores: Bacillus
Trichoderma
Lycopersicon esculentum
-Técnicas In Vitro
Controle Biológico de Vetores
Compostos de Amônio
Responsável: BR1942.1 - NID - Biblioteca - Núcleo de Informação e Documentação


  3 / 201 LILACS  
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Brandelli, Adriano
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Id: biblio-886885
Autor: COSTA, GÉSSICA A; ROSSATTO, FERNANDA C P; MEDEIROS, ALINE W; CORREA, ANA PAULA F; BRANDELLI, ADRIANO; FRAZZON, ANA PAULA G; MOTTA, AMANDA DE S DA.
Título: Evaluation antibacterial and antibiofilm activity of the antimicrobial peptide P34 against Staphylococcus aureus and Enterococcus faecalis
Fonte: An. acad. bras. ciênc;90(1):73-84, Mar. 2018. tab.
Idioma: en.
Resumo: ABSTRACT The adhesion ability of bacteria to abiotic surfaces has important implications in food industries, because these organisms can survive for long periods through the biofilm formation. They can be transferred from one place to another in the industry causing contamination of the food processing environment. In this study, the antibacterial and antibiofilm activities of the antimicrobial peptide P34, characterized as a bacteriocin-like substance (BLS P34) were tested against planktonic and sessile cells of Staphylococcus aureus and Enterococcus faecalis isolated from foods. The BLS P34 showed inhibitory effect against all planktonic cells of E. faecalis. The inhibition of biofilm formation and the eradication of pre-formed biofilm were evaluated with the crystal violet assay and with the reduction of 3-bromide [4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium. The BLS P34 promoted a reduction of percentage of adhered microbial cells on the surface, not being able to perform the complete elimination of biofilm formation. The metabolic activity of S. aureus biofilms decreased considerably between 41-95%. However, E. faecalis cells showed up metabolically stimulated. The BLS P34 has the potential antibiofilm for the species S. aureus. Studies suggest more detailed approaches to a better understanding of the interactions between the antimicrobial and bacterial cells within the biofilm structure.
Descritores: Oligopeptídeos/farmacologia
Staphylococcus aureus/efeitos dos fármacos
Bacteriocinas/farmacologia
Enterococcus faecalis/efeitos dos fármacos
Biofilmes/efeitos dos fármacos
Antibacterianos/farmacologia
-Bacillus/isolamento & purificação
Bacillus/metabolismo
Bacteriocinas/isolamento & purificação
Aderência Bacteriana/efeitos dos fármacos
Testes de Sensibilidade Microbiana
Análise de Variância
Limites: Animais
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


  4 / 201 LILACS  
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Id: biblio-1087345
Autor: Mostafa, Yasser; Alrumman, Suliman; Alamri, Saad; Hashem, Mohamed; Al-izran, Kholood; Alfaif, Mohammad; Elbehairi, Serag Eldin; Taha, Tarek.
Título: Enhanced production of glutaminase-free L-asparaginase by marine Bacillus velezensis and cytotoxic activity against breast cancer cell lines
Fonte: Electron. j. biotechnol;42:6-15, Nov. 2019. ilus, graf, tab.
Idioma: en.
Projeto: Deanship of Scientific Research, King Khalid University, Saudi Arabia.
Resumo: Background: The increasing rate of breast cancer globally requires extraordinary efforts to discover new effective sources of chemotherapy with fewer side effects. Glutaminase-free L-asparaginase is a vital chemotherapeutic agent for various tumor malignancies. Microorganisms from extreme sources, such as marine bacteria, might have high L-asparaginase productivity and efficiency with exceptional antitumor action toward breast cancer cell lines. Results: L-Asparaginase-producing bacteria, Bacillus velezensis isolated from marine sediments, were identified by 16S rRNA sequencing. L-Asparaginase production by immobilized cells was 61.04% higher than that by free cells fermentation. The significant productivity of enzyme occurred at 72 h, pH 6.5, 37°C, 100 rpm. Optimum carbon and nitrogen sources for enzyme production were glucose and NH4Cl, respectively. L-Asparaginase was free from glutaminase activity, which was crucial medically in terms of their severe side effects. The molecular weight of the purified enzyme is 39.7 KDa by SDS-PAGE analysis and was ideally active at pH 7.5 and 37°C. Notwithstanding, the highest stability of the enzyme was found at pH 8.5 and 70°C for 1 h. The enzyme kinetic parameters displayed Vmax at 41.49 µmol/mL/min and a Km of 3.6 × 10−5 M, which serve as a proof of the affinity to its substrate. The anticancer activity of the enzyme against breast adenocarcinoma cell lines demonstrated significant activity toward MDA-MB-231 cells when compared with MCF-7 cells with IC50 values of 12.6 ± 1.2 µg/mL and 17.3 ± 2.8 µg/mL, respectively. Conclusion: This study provides the first potential of glutaminase-free L-asparaginase production from the marine bacterium Bacillus velezensis as a prospect anticancer pharmaceutical agent for two different breast cancer cell lines.
Descritores: Asparaginase/metabolismo
Bacillus/enzimologia
Neoplasias da Mama/metabolismo
Glutaminase/metabolismo
-Asparaginase/biossíntese
Temperatura
Neoplasias da Mama/tratamento farmacológico
Cinética
Células Imobilizadas
Ensaios Enzimáticos
Fermentação
Células MCF-7
Concentração de Íons de Hidrogênio
Responsável: CL1.1 - Biblioteca Central


  5 / 201 LILACS  
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Id: lil-235870
Autor: Castro, J; García, I; Neyra, D.
Título: Eficacia del Bacillus sphaericus en criaderos naturales de mosquitos en zonas de alto riesgo de malaria / Efficacy of Bacillus sphaericus in natural fruitful of tippler in zones of risk high of malarie
Fonte: Rev. peru. epidemiol. (Online);9(2):34-9, dic. 1996. tab, graf.
Idioma: es.
Resumo: Desde 1985, en nuestro país, se ha incrementado el número de casos de malaria por Plasmodium falciparum, se han presentado brotes epidémicos en Loreto, Tumbes, Piura, San Martín, Bagua y Ucayali, alcanzando hasta fines de 1995 un acumulado a nivel nacional de 25, 210 casos. El Programa Nacional del Control de la Malaria, preocupado por esta situación ha diseñado planes operativos de control vectorial, contra la malaria y para ello, ha considerado, además el control químio, la introducción de nuevos métodos de lucha: la gestión ambiental, control biológico y cultural. Los métodos químicos que se vienen utilizando desde hace mas de 40 años, han ocasionado resistencia en las especies vectoras, así como un comportamiento refractario; además de haber provocado transtornos ecológicos irreversibles por su efecto acumulativo. El Control Biológico, es una alternativa que ofrece ventajas por su tendencia a hacerse permanente, lento efecto represivo y fácil aplicación. De los métodos de control biológico, el bacteriológico, ha sido seleccionado para dar inicio al tratamiento de los criaderos naturales, con especial énfasis en las áreas de alta transmisión de malaria. Se seleccionaron las sub-regiones de La Libertad, Piura, Sullana, Tumbes y San Martín, identificándose 38 criaderos en su jurisdicción. Se aplicó el biolarvicida, cuyo principio activo son esporas y cristales del Bacillus sphaericus, alcanzando a las 48 horas una efectividad del 90 por ciento para todos los estadíos larvarios y una eficiencia del 94 por ciento en 4 de las 5 Sub-regiones de Salud estudiadas. No se observó ningún daño sobre los hidrobiontes que acompañaron a los criaderos.
Descritores: Plasmodium malariae
Bacillus
Surtos de Doenças
Culicidae
Responsável: PE1.1 - Oficina Universitária de Biblioteca


  6 / 201 LILACS  
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Timenetsky, Jorge
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Id: biblio-1056903
Autor: Franco, Mariane F; Gaeta, Natália C; Alemán, Mario A. R; Mellville, Priscilla A; Timenetsky, Jorge; Balaro, Mário F. A; Gregory, Lilian.
Título: Bacteria isolated from the lower respiratory tract of sheep and their relationship to clinical signs of sheep respiratory disease / Bactérias detectadas no trato respiratório inferior de ovinos e a relação com as manifestações clínicas da doença respiratória ovina
Fonte: Pesqui. vet. bras = Braz. j. vet. res;39(10):796-801, Oct. 2019. tab.
Idioma: en.
Resumo: Respiratory diseases are among the most important diseases in sheep flocks. Herein was studied the bacterial etiology of respiratory disease and the clinical signs of 99 female and male sheep breed in the states of São Paulo (SP) and Rio de Janeiro (RJ), Brazil. After physical examination of animals, tracheobronchial flushing samples were obtained. The usual bacteria and Mycoplasma spp. were searched, as well as their association with the clinical status and clinical signs of sheep with respiratory disease. The main observed signs were: tachypnea (75%), increase of rectal temperature (09.4%), mucopurulent/purulent nasal discharge (21.9%), cough (25%), dyspnea (31.2%), changes of lung sounds at auscultation (87.5%) and chest percussion (28.1%) in pneumonic sheep. Non-fermenting gram-negative bacteria and Bacillus sp. were the most isolated bacteria. Microorganisms of the Mollicutes class were molecularly (PCR) detected in 33.3% of the animals. In addition, the specific detection of M. mycoides subsp. capri was described for the first time in sheep from the state of São Paulo, Brazil.(AU)

A doença respiratória é uma das doenças mais importantes em rebanhos ovinos. Esta pesquisa teve como objetivo determinar a etiologia bacteriana da doença respiratória e sua relação com sinais clínicos em ovinos criados nos estados de São Paulo e Rio de Janeiro, Brasil. Noventa e nove ovelhas machos e fêmeas dos Estados de São Paulo (SP) e Rio de Janeiro (RJ) foram estudadas. Após o exame físico, amostras de lavagem traqueobrônquica foram obtidas. A presença de bactérias aeróbias e Mycoplasmaspp. foram estudados, assim como a associação entre os microrganismos e estado clínico e sinais clínicos de doença respiratória em ovinos. As principais manifestações clínicas observadas foram: taquipneia (75%), alta temperatura retal (09,4%), secreção nasal mucopurulenta/purulenta (21,9%), tosse (25%), dispneia (31,2%), sons pulmonares alterados na ausculta (87,5%) e na percussão torácica (28,1%) em ovelhas pneumônicas. Bactérias gram-negativas não fermentadoras e Bacillus sp. foram as bactérias mais isoladas. Microrganismos da classe Mollicutes foram detectados molecularmente (PCR) em 33,3% dos ovinos. Além disso, descreve-se pela primeira vez no estado de São Paulo, Brasil, a detecção do M. mycoides subsp. capri na espécie ovina utilizando a reação de polimerase em cadeia.(AU)
Descritores: Infecções por Pasteurella/veterinária
Pneumonia/etiologia
Pneumonia/veterinária
Ovinos
Infecções por Mycoplasma/veterinária
-Doenças dos Ovinos/microbiologia
Bacillus/isolamento & purificação
Pasteurella/isolamento & purificação
Klebsiella/isolamento & purificação
Mycoplasma/isolamento & purificação
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


  7 / 201 LILACS  
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Id: biblio-951792
Autor: Moghannem, Saad A. M; Farag, Mohamed M. S; Shehab, Amr M; Azab, Mohamed S.
Título: Exopolysaccharide production from Bacillus velezensis KY471306 using statistical experimental design
Fonte: Braz. j. microbiol;49(3):452-462, July-Sept. 2018. tab, graf.
Idioma: en.
Resumo: Abstract Exopolysaccharide (EPS) biopolymers produced by microorganisms play a crucial role in the environment such as health and bio-nanotechnology sectors, gelling agents in food and cosmetic industries in addition to bio-flocculants in the environmental sector as they are degradable, nontoxic. This study focuses on the improvement of EPS production through manipulation of different culture and environmental conditions using response surface methodology (RSM). Plackett-Burman design indicated that; molasses, yeast extract and incubation temperature are the most effective parameters. Box-Behnken RSM indicated that; the optimum concentration for each parameter was 12% (w/v) for molasses, 6 g/L yeast extract and 30 °C for incubation temperature. The most potent bacterial isolate was identified as Bacillus velezensis KY498625. After production, EPS was extracted, purified using DEAE-cellulose, identified using Fourier transform infrared (FTIR), gel permeation chromatography (GPC) and gas chromatography-mass spectroscopy (GC-MS). The result indicated that; it has molecular weight 1.14 × 105 D consisting of glucose, mannose and galactose.
Descritores: Polissacarídeos Bacterianos/metabolismo
Bacillus/metabolismo
-Polissacarídeos Bacterianos/isolamento & purificação
Polissacarídeos Bacterianos/química
Bacillus/química
Microbiologia Industrial
Espectroscopia de Infravermelho com Transformada de Fourier
Meios de Cultura/metabolismo
Meios de Cultura/química
Peso Molecular
Responsável: BR1.1 - BIREME


  8 / 201 LILACS  
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Id: biblio-974339
Autor: Ribeiro, Vitória Palhares; Marriel, Ivanildo Evódio; Sousa, Sylvia Morais de; Lana, Ubiraci Gomes de Paula; Mattos, Bianca Braz; Oliveira, Christiane Abreu de; Gomes, Eliane Aparecida.
Título: Endophytic Bacillus strains enhance pearl millet growth and nutrient uptake under low-P
Fonte: Braz. j. microbiol;49(supl.1):40-46, 2018. tab.
Idioma: en.
Projeto: Fapemig; . MPCPAgro; . Embrapa.
Resumo: Abstract Bacterial endophytes are considered to have a beneficial effect on host plants, improving their growth by different mechanisms. The objective of this study was to investigate the capacity of four endophytic Bacillus strains to solubilize iron phosphate (Fe-P), produce siderophores and indole-acetic acid (IAA) in vitro, and to evaluate their plant growth promotion ability in greenhouse conditions by inoculation into pearl millet cultivated in a P-deficient soils without P fertilization, with Araxá rock phosphate or soluble triple superphosphate. All strains solubilized Fe-P and three of them produced carboxylate-type siderophores and high levels of IAA in the presence of tryptophan. Positive effect of inoculation of some of these strains on shoot and root dry weight and the N P K content of plants cultivated in soil with no P fertilization might result from the synergistic combination of multiple plant growth promoting (PGP) traits. Specifically, while B1923 enhanced shoot and root dry weight and root N P content of plants cultivated with no P added, B2084 and B2088 strains showed positive performance on biomass production and accumulation of N P K in the shoot, indicating that they have higher potential to be microbial biofertilizer candidates for commercial applications in the absence of fertilization.
Descritores: Bacillus/metabolismo
Alimentos/metabolismo
Pennisetum/crescimento & desenvolvimento
Pennisetum/microbiologia
Endófitos/metabolismo
Ácidos Indolacéticos/metabolismo
-Fosfatos/análise
Fosfatos/metabolismo
Bacillus/genética
Sideróforos/metabolismo
Raízes de Plantas/crescimento & desenvolvimento
Raízes de Plantas/metabolismo
Raízes de Plantas/microbiologia
Pennisetum/metabolismo
Endófitos/genética
Ferro/metabolismo
Responsável: BR1.1 - BIREME


  9 / 201 LILACS  
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Id: biblio-974338
Autor: Tahir, Muhammad; Khalid, Umaira; Ijaz, Muhammad; Shah, Ghulam Mustafa; Naeem, Muhammad Asif; Shahid, Muhammad; Mahmood, Khalid; Ahmad, Naveed; Kareem, Fazal.
Título: Combined application of bio-organic phosphate and phosphorus solubilizing bacteria (Bacillus strain MWT 14) improve the performance of bread wheat with low fertilizer input under an arid climate
Fonte: Braz. j. microbiol;49(supl.1):15-24, 2018. tab, graf.
Idioma: en.
Resumo: Abstract This study was aimed to investigate the effect of bio-organic phosphate either alone or in combination with phosphorus solubilizing bacteria strain (Bacillus MWT-14) on the growth and productivity of two wheat cultivars (Galaxy-2013 and Punjab-2011) along with recommended (150-100 NP kg ha−1) and half dose (75-50 NP kg ha−1) of fertilizers. The combined application of bio-organic phosphate and the phosphorous solubilizing bacteria strain at either fertilizer level significantly improved the growth, yield parameters and productivity of both wheat cultivars compared to non-inoculated control treatments. The cultivar Punjab-2011 produced the higher chlorophyll contents, crop growth rate, and the straw yield at half dose of NP fertilizer; while Galaxy-2013, with the combined application of bio-organic phosphate and phosphorous solubilizing bacteria under recommended NP fertilizer dose. Combined over both NP fertilizer levels, the combined use of bio-organic phosphate and phosphorous solubilizing bacteria enhanced the grain yield of cultivar Galaxy-2013 by 54.3% and that of cultivar Punjab-2011 by 83.3%. The combined application of bio-organic phosphate and phosphorous solubilizing bacteria also increased the population of phosphorous solubilizing bacteria, the soil organic matter and phosphorous contents in the soil. In conclusion, the combined application of bio-organic phosphate and phosphorous solubilizing bacteria offers an eco-friendly option to harvest the better wheat yield with low fertilizer input under arid climate.
Descritores: Fosfatos/farmacocinética
Fósforo/metabolismo
Bacillus/metabolismo
Triticum/crescimento & desenvolvimento
Fertilizantes/análise
Produção Agrícola/métodos
-Fosfatos/análise
Fósforo/análise
Microbiologia do Solo
Triticum/metabolismo
Triticum/microbiologia
Clima
Responsável: BR1.1 - BIREME


  10 / 201 LILACS  
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Id: biblio-974335
Autor: Naeem, Muhammad; Aslam, Zubair; Khaliq, Abdul; Ahmed, Jam Nazir; Nawaz, Ahmad; Hussain, Mubshar.
Título: Plant growth promoting rhizobacteria reduce aphid population and enhance the productivity of bread wheat
Fonte: Braz. j. microbiol;49(supl.1):9-14, 2018. tab, graf.
Idioma: en.
Resumo: Abstract Plant growth promoting rhizobacteria increase plant growth and give protection against insect pests and pathogens. Due to the negative impact of chemical pesticides on environment, alternatives to these chemicals are needed. In this scenario, the biological methods of pest control offer an eco-friendly and an attractive option. In this study, the effect of two plant growth promoting rhizobacterial strains (Bacillus sp. strain 6 and Pseudomonas sp. strain 6K) on aphid population and wheat productivity was evaluated in an aphid susceptible (Pasban-90) and resistant (Inqlab-91) wheat cultivar. The seeds were inoculated with each PGPR strain, separately or the combination of both. The lowest aphid population (2.1 tiller−1), and highest plant height (85.8 cm), number of spikelets per spike (18), grains per spike (44), productive tillers (320 m−2), straw yield (8.6 Mg ha−1), and grain yield (4.8 Mg ha−1) were achieved when seeds were inoculated with Bacillus sp. strain 6 + Pseudomonas sp. strain 6K. The grain yield of both varieties was enhanced by 35.5-38.9% with seed inoculation with both bacterial strains. Thus, the combine use of both PGPR strains viz. Bacillus sp. strain 6 + Pseudomonas sp. strain 6K offers an attractive option to reduce aphid population tied with better wheat productivity.
Descritores: Afídeos/crescimento & desenvolvimento
Pseudomonas/fisiologia
Bacillus/fisiologia
Triticum/crescimento & desenvolvimento
-Doenças das Plantas/parasitologia
Doenças das Plantas/prevenção & controle
Microbiologia do Solo
Triticum/microbiologia
Triticum/parasitologia
Controle Biológico de Vetores
Dinâmica Populacional
Limites: Animais
Responsável: BR1.1 - BIREME



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