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Id: biblio-1118082
Autor: Iurkiv, Luciana; Eckstein, Barbara; Lorenzetti, Eloisa; Stangarlin, José Renato.
Título: Biotic and abiotic resistance inducers for controlling white rust in rocket (Eruca sativa) / Indutores de resistência bióticos e abióticos para controle de ferrugem branca em rúcula (Eruca sativa)
Fonte: Arq. Inst. Biol;87:e0202019, 2020. tab, graf.
Idioma: en.
Resumo: Induced resistance emerges as an alternative method for controlling plant diseases. This study aimed to evaluate the efficacy of biotic and abiotic resistance inducers for controlling white rust in rocket (Eruca sativa), as well as biochemical changes (peroxidase) and fitness costs. The experiments were developed with the abiotic inducers acibenzolar-S-methyl (ASM) (12.5, 25, and 50 mg ai L-1) and citrus biomass (CB) (0.1, 0.25, and 0.5%), as well as with the biotic ones Saccharomyces cerevisiae (25 mg mL-1), Bacillus thuringiensis (25 mg p.c. mL-1), Saccharomyces boulardii (25 mg mL-1), and phosphorylated mannan oligosaccharide (PMO) (0.25%), in preventive and curative interventions. Fungicide mancozeb (1.6 g ai L-1), Bordeaux mixture (1%), and water were the control treatments. Leaf samples were collected 3, 7, 11, 15, and 19 days after the treatments to determine peroxidases and assess the severity and production. Concerning abiotic inducers, all doses of ASM and CB 0.5% (preventive) and CB 0.25% (curative) reduced the severity of white rust, whereas, among biotic inducers, only PMO applied preventively controlled the disease. Peroxidase activity was higher for CB 0.25% and ASM 50 mg L-1. Bordeaux mixture induced higher peroxidase activity.(AU)

A indução de resistência surge como um método alternativo para o controle de doenças em plantas. O objetivo deste trabalho foi avaliar a eficiência de indutores de resistência bióticos e abióticos no controle de ferrugem branca em rúcula (Eruca sativa), bem como alterações bioquímicas (peroxidase) e o impacto na produção. Foram desenvolvidos experimentos com os indutores abióticos acibenzolar-S-metil (ASM) (12,5; 25 e 50 mg i.a. L-1) e biomassa cítrica (BC) (0,1; 0,25 e 0,5%), e os bióticos Saccharomyces cerevisiae (25 mg p.c. mL-1), Bacillus thuringiensis (25 mg p.c. mL-1), Saccharomyces boulardii (25 mg p.c. mL-1) e manano-oligossacarídeo fosforilado (MOF) (0,25%), sendo aplicados preventiva e curativamente. Como controles foram utilizados o fungicida mancozeb (1,6 g i.a. L-1), calda bordalesa (1%) e água. Amostras de folhas foram coletadas aos 3, 7, 11, 15 e 19 dias após os tratamentos para a determinação de peroxidases, e avaliações de severidade e produção. Entre os indutores abióticos, todas as doses de ASM e BC 0,5% (preventivamente) e BC 0,25% (curativamente) reduziram a severidade da ferrugem branca, enquanto entre os indutores bióticos, apenas o MOF aplicado preventivamente, controlou a doença. A atividade de peroxidase foi superior para BC 0,25% e ASM 50 mg L-1. O tratamento com calda bordalesa também incrementou a atividade de peroxidase.(AU)
Descritores: Doenças das Plantas
Saccharomyces
Bacillus thuringiensis
-Peroxidase
Brassicaceae
Eficiência
Responsável: BR1942.1 - NID - Biblioteca - Núcleo de Informação e Documentação


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Id: lil-619858
Autor: Masuh, H; Seccacini, E; De Licastro, S; Zerba, E.
Título: Residualidad de un formulado sólido del insecticida microbiano Bti (H-14) en el control de larvas de Aedes aegypti (Diptera: Culicidae) / Residuality of a microbial insecticide solid formulated Bti (H-14) in the
Fonte: Rev. peru. epidemiol. (Online);10(1):1-3, 2002. graf.
Idioma: es.
Resumo: El Bacillus thuringiensis var. israelensis (Bti ó Bti H-14) produce una toxina proteica, la cual constituye una herramienta de gran utilidad para el control de larvas de Aedes aegypti. Una suspensión líquida de la toxina y las esporas (Bti) ha sido utilizada en campañas nacionales de control del mosquito vector del Dengue en Argentina, pero la falta de efecto residual constituye elmayor inconveniente para su aplicación. En la búsqueda de nuevas estrategias para el control de vectores de enfermedades, este trabajo presenta algunos resultados sobre una nueva formulación de liberación controlada de Bti, denominada ôMosquito Dunks®õ. Se utilizaron larvas de Ae. aegypti del III estadio tardío ó IV temprano (cepa susceptible CIPEIN) que en ensayos de laboratorio demostraron una actividad residual a dosis de uso recomendadas por el fabricante (14,4 mg/cm2) de al menos 180 días con mortalidad total luego de 48 horas de exposición. Los resultados que se presentan en este trabajo sugieren que la nueva formulación sólida de liberación lenta de Bti, debido a su residualidad representa una buena alternativa para el reemplazo del Temefos en el control de larvas de Aedes aegypti, especialmente en aquellos casos donde se detecte resistencia al larvicida organofosforado.

The entomopathogenous agent Bacillus thuringensis var. israelensis (Bti or Bti H-14), produces a proteic toxin that is a promissory tool to use in chemical control of larvae of Aedes aegypti. Bti (H-14) as a liquid suspension, has been used in national campaigns against Ae. aegypti , vector of Dengue disease, but its lack of residual effect is a major inconvenient. In the search of new strategies in vector control, this paper presents some results of an alternative slow release formulation of Bti (H-14), the ôMosquito Dunksõ . III instar or young IV instar larvae of a susceptible strain of Aedes aegypti (CIPEIN strain) was used in laboratory assays. The ôMosquito Dunksõ at recommended use dosis (14,4 mg / 100 cm2 surface) were assayed for residual activity. 100% mortality was found for at least 180 days (6 month) with 48 hs larvae exposition. The results presented in this work suggests that this new solid slow release formulation of Bti with more residual activity could be a good alternative for Temephos for Aedes aegypti larvae control, specially for those cases in which resistance to the organophosphorus compound is reported.
Descritores: Aedes
Bacillus thuringiensis
Culicidae
Dengue/prevenção & controle
Densovirinae
Responsável: PE1.1 - Oficina Universitária de Biblioteca


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Id: lil-386680
Autor: Cavados, C. F. G; Majerowicz, S; Chaves, J. Q; Araújo-Coutinho, C. J. P. C; Rabinovitch, L.
Título: Histopathological and ultrastructural effects of delta-endotoxins of Bacillus thuringiensis serovar israelensis in the midgut of Simulium pertinax larvae (Diptera, Simuliidae)
Fonte: Mem. Inst. Oswaldo Cruz;99(5):493-498, Aug. 2004. ilus.
Idioma: en.
Resumo: The bacterium Bacillus thuringiensis (Bt) produces parasporal crystals containing delta-endotoxins responsible for selective insecticidal activity on larvae. Upon ingestion, these crystals are solubilized in the midgut lumen and converted into active toxins that bind to receptors present on the microvilli causing serious damage to the epithelial columnar cells. We investigated the effect of these endotoxins on larvae of the Simulium pertinax, a common black fly in Brazil, using several concentrations during 4 h of the serovar israelensis strain IPS-82 (LFB-FIOCRUZ 584), serotype H-14 type strain of the Institute Pasteur, Paris. Light and electron microscope observations revealed, by time and endotoxin concentration, increasing damages of the larvae midgut epithelium. The most characteristic effects were midgut columnar cell vacuolization, microvilli damages, epithelium cell contents passing into the midgut lumen and finally the cell death. This article is the first report of the histopathological effects of the Bti endotoxins in the midgut of S. pertinax larvae and the data obtained may contribute to a better understanding of the mode of action of this bacterial strain used as bioinsecticide against black fly larvae.
Descritores: Simuliidae
Bacillus thuringiensis
Sistema Digestório
Inseticidas
-Microscopia Eletrônica
Controle Biológico de Vetores
Larva
Limites: Animais
Responsável: BR1.1 - BIREME


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Mello, Rubens Pinto de
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Id: lil-344292
Autor: Araújo-Coutinho, Carlos José Pereira da Cunha de; Cunha, Andrea de Barros Pinto Viviani; Serra-Freire, Nicolau Maués; Mello, Rubens Pinto de.
Título: Evaluation of the impact of Bacillus thuringiensis serovar israelensis and Temephos, used for the control of Simulium (Chirostilbia) pertinax Kollar, 1832 (Diptera, Simuliidae) on the associated entomofauna, Paraty, state of Rio de Janeiro, Brazil
Fonte: Mem. Inst. Oswaldo Cruz;98(5):697-702, July 2003. tab.
Idioma: en.
Resumo: The study was set up to evaluate the impact of two commercial larvicide formulations, Bacillus thuringiensis serovar israelensis base (Bti) at 15 ppm/1 min and temephos at 0.03 ppm of active ingredient, used to control Simulium pertinax populations, on associated non-target entomofauna occupying the same breeding sites. The experiments were carried out on the Pedra Branca and Muricana rivers, on the slopes of Serra do Mar massif, municipality of Paraty, state of Rio de Janeiro, Brazil. Bti was applied to the river Pedra Branca and temephosto theriver Muricana. On both rivers, treatment and control sections were labeled as such, each one with two observation posts: slow moving water and fast water regions respectively. Artificial substrata was used to evaluate the abundance of associated entomofauna. Attached immature stages of arthropods were removed from both of its surfaces fortnightly. Were collected, from the two rivers, 28 477 specimens of the entomofauna associated with S. pertinax. The families Hydropsychidae, Chironomidae, Bactidae, Simuliidae, Blephariceridae and Megapodagrionidae were represented. These was an impact of temephos on the entomofauna associated with S. pertinax only in Simuliidae and Chironomidae, and to Bti only in Simuliidae. However, the reduction in their numbers was not statistically significant
Descritores: Artrópodes
Bacillus thuringiensis
Temefós
Monitoramento Ambiental
Inseticidas Organofosforados
-Simuliidae
Temperatura
Movimentos da Água
Poluentes Químicos da Água
Brasil
Controle de Mosquitos
Densidade Demográfica
Concentração de Íons de Hidrogênio
Larva
Limites: Animais
Responsável: BR1.1 - BIREME


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Id: lil-298881
Autor: Cavados, Cfg; Fonseca, Rn; Chaves, Jq; Rabinovitch, L; Araújo-Coutinho, Cjpc.
Título: Identification of entomopathogenic Bacillus isolated from Simulium (Diptera, Simuliidae) larvae and adults
Fonte: Mem. Inst. Oswaldo Cruz;96(7):1017-1021, Oct. 2001. ilus, tab.
Idioma: en.
Resumo: Entomopathogenic bacteria isolated from Simulium larvae and adults from breeding sites in the states of Säo Paulo and Rio de Janeiro, Brazil, were identified as 18 strains of Bacillus thuringiensis and one of B. sphaericus. Most of these strains were serotyped according to their flagellar antigens. However, nine of the B. thuringiensis samples, could not be serotyped and were designated as "autoagglutinating"; they were also shown to be toxic in preliminary tests against Aedes aegypti larvae. Additionally, B. sphaericus was also shown to be toxic towards Culex quinquefasciatus larvae
Descritores: Simuliidae/parasitologia
Bacillus thuringiensis/isolamento & purificação
-Bacillus thuringiensis/classificação
Microbiologia da Água
Brasil
Sorotipagem
Controle de Mosquitos
Culex
Eletroforese em Gel de Poliacrilamida
Insetos Vetores
Larva
Limites: Animais
Responsável: BR1.1 - BIREME


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Rabinovitch, Leon
Cavados, Clara de Fátima G
Zahner, Viviane
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Id: lil-241333
Autor: Rabinovitch, Leon; Cavados, Clara de Fátima G; Chaves, Jeane Q; Coutinho, Carlos José PCA; Zahner, Viviane; Silva, Katia Regina A; Seldin, Lucy.
Título: A new strain of Bacillus thuringiensis Serovar israelensis very active against blackfly larvae
Fonte: Mem. Inst. Oswaldo Cruz;94(5):683-5, Sept. 1999. ilus.
Idioma: en.
Descritores: Simuliidae/efeitos dos fármacos
Bacillus thuringiensis
Controle de Insetos/métodos
Inseticidas/farmacologia
Culicidae/efeitos dos fármacos
-Larva
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-321771
Autor: Anon.
Título: Biological control program against simuliids in the State of São Paulo, Brazil
Fonte: Mem. Inst. Oswaldo Cruz;90(1):131-133, Jan.-Feb. 1995.
Idioma: en.
Resumo: In Brazil, the use of biological vector-control methods has been largely confined to experimental research, with little or no application of such techniques by public institutions responsible for implementing control programs. The notable exceptions have been the black fly control program carried out by the Health Secretariat in the State of São Paulo. Since the 1980s, São Paulo's "Superintendência de Controle de Endemias" has been conducting studies on the viability of using Bacillus thuringiensis (H-14) for simuliid control, and the results have been so encouraging that the agency has now incorporated this method into its Simuliid Control Program.
Descritores: Simuliidae
Bacillus thuringiensis
Controle Biológico de Vetores/métodos
-Brasil
Limites: Animais
Humanos
Responsável: BR1.1 - BIREME


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Id: lil-419709
Autor: Cavados, Clara F. G; Fonseca, Rodrigo N; Chaves, Jeane Q; Araújo-Coutinho, Carlos J. P. C; Rabinovitch, Leon.
Título: A new black fly isolate of Bacillus thuringiensis autoagglutinating strain highly toxic to Simulium pertinax (Kollar) (Diptera, Simuliidae) larvae
Fonte: Mem. Inst. Oswaldo Cruz;100(7):795-797, Nov. 2005.
Idioma: en.
Projeto: Fiocruz.
Resumo: Formulations containing the entomopathogenic Bacillus thuringiensis serovar israelensis strain IPS-82 has been widely applied for mosquito control around the world. Strain IPS-82 is highly active against Aedes aegypti but less active against other well-known vectors such as Culex quinquefasciatus and Simulium spp. larvae. Eighteen strains of B. thuringiensis were isolated from Simulium pertinax larvae naturally occurring in rivers of Southeast Brazil with one demonstrating special toxic effects. Simulated field tests against S. pertinax larvae showed that the native Brazilian autoagglutinanting B. thuringiensis (LFB-FIOCRUZ 1035) has an LC50 at least 25 times lower than the standard IPS-82 strain. The same bacterial preparation was also tested against Ae. aegypti larvae in laboratory trials and the LC50 values obtained with LFB-FIOCRUZ 1035 were at least three times lower than the one for the IPS 82 strain. The results indicate that this strain is more toxic than the standard B. thuringiensis serovar israelensis (H14) in the two Dipteran species tested. It is noteworthy that differences between LC50 values were more pronounced in S. pertinax larvae, the source of the original isolation.
Descritores: Aedes/efeitos dos fármacos
Bacillus thuringiensis/isolamento & purificação
Toxinas Bacterianas/toxicidade
Controle Biológico de Vetores/métodos
Simuliidae/efeitos dos fármacos
-Aglutinação
Bacillus thuringiensis/química
Toxinas Bacterianas/isolamento & purificação
Larva/efeitos dos fármacos
LETHAL DOSE ACADEMIES AND INSTITUTES
Limites: Animais
Tipo de Publ: Estudo Comparativo
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-788966
Autor: Azizoglu, Ugur; Ayvaz, Abdurrahman; Yılmaz, Semih; Karabörklü, Salih; Temizgul, Rıdvan.
Título: Expression of cry1Ab gene from a novel Bacillus thuringiensis strain SY49-1 active on pest insects
Fonte: Braz. j. microbiol;47(3):597-602, July-Sept. 2016. graf.
Idioma: en.
Projeto: Erciyes University; . Bilim, Sanayi ve Teknoloji Bakanlığı.
Resumo: ABSTRACT In this study, the cry1Ab gene of previously characterized and Lepidoptera-, Diptera-, and Coleoptera-active Bacillus thuringiensis SY49-1 strain was cloned, expressed and individually tested on Ephestia kuehniella (Lepidoptera: Pyralidae) and Plodia interpunctella (Lepidoptera: Pyralidae) larvae. pET-cry1Ab plasmids were constructed by ligating the cry1Ab into pET28a (+) expression vector. Constructed plasmids were transferred to an Escherichia coli BL21 (DE3) strain rendered competent with CaCl2. Isopropyl β-D-1-thiogalactopyranoside was used to induce the expression of cry1Ab in E. coli BL21(DE3), and consequently, ∼130 kDa of Cry1Ab was obtained. Bioassay results indicated that recombinant Cry1Ab at a dose of 1000 µg g-1 caused 40% and 64% mortality on P. interpunctella and E. kuehniella larvae, respectively. However, the mortality rates of Bt SY49-1 strains' spore-crystal mixture at the same dose were observed to be 70% on P. interpunctella and 90% on E. kuehniella larvae. The results indicated that cry1Ab may be considered as a good candidate in transgenic crop production and as an alternative biocontrol agent in controlling stored product moths.
Descritores: Bacillus thuringiensis/fisiologia
Proteínas de Bactérias/genética
Expressão Gênica
Controle de Insetos
Endotoxinas/genética
Proteínas Hemolisinas/genética
-Bacillus thuringiensis/ultraestrutura
Proteínas de Bactérias/metabolismo
Proteínas de Bactérias/toxicidade
Controle de Insetos/métodos
Clonagem Molecular
Endotoxinas/metabolismo
Endotoxinas/toxicidade
Proteínas Hemolisinas/metabolismo
Proteínas Hemolisinas/toxicidade
Inseticidas
Larva
Mariposas/efeitos dos fármacos
Limites: Animais
Responsável: BR1.1 - BIREME


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Id: biblio-1024585
Autor: Colombo, Fernanda Caroline; Maciel, Rodrigo Mendes Antunes; Libardoni, Gabriela; Raulino, Fernanda; Lozano, Everton Ricardi; Maia, Fabiana Martins Costa; Freitas, Patrícia Franchi; Neves, Pedro Manuel Oliveira Janeiro; Potrich, Michele.
Título: Longevity of Apis mellifera workers fed on a diet incorporating entomopathogens / Longevidade de operárias de Apis mellifera alimentadas com dieta incorporada com entomopatógenos
Fonte: Arq. Inst. Biol;86:e0492017, 2019. ilus, tab.
Idioma: en.
Projeto: Araucária Foundation.
Resumo: The present study had the objective of evaluating the longevity of A. mellifera workers fed on a diet incorporating commercial entomopathogens, Beauveria bassiana, and Bacillus thuringiensis. It also aimed at verifying possible morphological alterations in the midgut. To this purpose, the entomopathogens used were B. bassiana (Product A) (5.0 × 1011 viable conidia.kg-1), B. thuringiensis (Product B) (2.5 × 109 viable spores.g-1), and B. thuringiensis (Product C) (1.0 × 109 viable spores.g-1); and two controls: T1: sterilized distilled water, and T2: sterilized distilled water + Tween 80® (0.01%). For the bioassays, 2 mL of each treatment were incorporated into Candy paste. For each treatment, 80 bees were individually in flat bottom glass tubes (2.5 cm Ø) covered with voile, containing a piece of cotton soaked in water and Candy paste. These tubes were stored in a B.O.D (30 ± 2°C, R.H 70% ± 10%, 12 h), and mortality was evaluated every six hours, for 10 days. Soon after verifying mortality, two bees per treatment were selected for the removal of their midgut. Midgut samples were processed using standard methodology for Scanning Electron Microscopy (SEM). It was verified that products A, B, and C reduced the longevity of bees when compared to T1 and T2 controls. In the qualitative analyses carried out using SEM, it was not possible to observe external or internal morphological alterations to midgut tissues. Although products A, B, and C cause a reduction in longevity, their presence was not verified when tissues were analyzed using SEM.(AU)

No presente trabalho objetivou-se avaliar a longevidade de operárias de A. mellifera alimentadas com dieta incorporada com os entomopatógenos comerciais Beauveria bassiana e Bacillus thuringiensis, e verificar possíveis alterações morfológicas em seu mesêntero. Para isso, os entomopatógenos utilizados foram B. bassiana (Produto A) (5,0 × 1011 conídios viáveis.kg-1), B. thuringiensis (Produto B) (2,5 × 109 esporos viáveis.g-1), B. thuringiensis (Produto C) (1,0 × 109 esporos viáveis.g-1); e dois controles: T1: água destilada esterilizada e T2: água destilada esterilizada + Tween 80® (0,01%). Para os bioensaios, 2 mL de cada tratamento foram incorporados à pasta Cândi. Para cada tratamento, 80 abelhas foram acondicionadas, individualmente, em tubos de vidro de fundo chato (2,5 cm Ø), cobertos com voile, contendo um pedaço de algodão embebido em água e pasta Cândi. Os tubos contendo as abelhas foram acondicionados em B.O.D (30 ± 2°C, U.R. 70% ± 10%, 12 h), e a mortalidade foi avaliada a cada seis horas, durante 10 dias. Logo após a verificação da mortalidade, foram separadas duas abelhas por tratamento para a retirada do mesêntero. Essas amostras foram processadas em metodologia padrão para Microscopia Eletrônica de Varredura (MEV). Verificou-se que os produtos A, B e C reduziram a longevidade das abelhas quando comparados aos controles T1 e T2. Nas análises qualitativas realizadas com MEV, não foi possível observar alterações morfológicas externas ou internas nos tecidos do mesêntero. Apesar dos produtos A, B e C causarem redução na longevidade, sua presença não foi verificada quando os tecidos foram analisados por MEV.(AU)
Descritores: Abelhas
Longevidade
-Bacillus thuringiensis
Beauveria
Responsável: BR1942.1 - NID - Biblioteca - Núcleo de Informação e Documentação



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