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Id: biblio-1130108
Autor: Tomaseto, Alex Augusto; Alpiste, Marcel Costa; Nassar, Alessandra Figueiredo de Castro; Destéfano, Suzete Aparecida Lanza.
Título: Antibacterial activity of phytopathogenic Streptomyces strains against bacteria associated to clinical diseases / Atividade antimicrobiana de Streptomyces fitopatogênicas contra bactérias associadas a doenças de importância clínica
Fonte: Arq. Inst. Biol;87:e0142020, 2020. tab.
Idioma: en.
Projeto: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil; . Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
Resumo: The genus Streptomyces is associated with the ability to produce and excrete a variety of bioactive compounds, such as antibiotic, antifungal and antiviral. Biological active polyketide and peptide compounds with applications in medicine, agriculture and biochemical research are synthesized by PKS-I and NRPS genes. The evaluation of the presence of these genes associated with the biosynthesis of secondary metabolites in different phytopathogenic Streptomyces strains were performed using degenerated primers. The positive signal was observed in 58/63 Streptomyces strains for NRPS gene, 43/63 for PKS-I, and for PKS-II all the 63 strains showed positive signal of amplification. These strains also were tested with double layer agar-well technique against bacterial with clinical importance, and it was possible to observe the Streptomyces spp. strains were able to inhibit the growth of 14, 20, 13 and 3 isolates Gram-positive and Gram-negative bacteria, Staphylococcus aureus (ATCC 25923), Bacillus cereus (ATCC 14579), Pseudomonas aeruginosa (ATCC 27853) and Escherichia coli (ATCC 11775) respectively. The Streptomyces sp. strains IBSBF 2019 and IBSBF 2397 showed antibacterial activity against all four bacteria-target tested.(AU)

O gênero Streptomyces apresenta alta capacidade de produzir e excretar uma grande variedade de compostos biologicamente ativos, como antibióticos, antifúngicos e antivirais. Compostos biologicamente ativos de policetídeos e peptídeos com aplicações na medicina, agricultura e pesquisas bioquímicas são sintetizados pelos genes PKS-I e NRPS. A avaliação da presença desses genes associados à biossíntese de metabólitos secundários em diferentes linhagens de Streptomyces fitopatogênicas foi realizada através do uso de primers degenerados. O sinal positivo foi observado em 58/63 linhagens de Streptomyces para o gene NRPS, 43/63 para o gene PKS-I e, para o gene PKS-II, todas as 63 linhagens apesentaram o sinal positivo de amplificação. Essas linhagens também foram testadas através da técnica de dupla camada contra bactérias de importância clínica e foi possível observar que as linhagens de Streptomyces spp. foram capazes de inibir o crescimento de 14, 20, 13 e 3 isolados de bactérias Gram-positivas e Gram-negativas, Staphylococcus aureus (ATCC 25923), Bacillus cereus (ATCC 14579), Pseudomonas aeruginosa (ATCC 27853) e Escherichia coli (ATCC 11775), respectivamente. As linhagens de Streptomyces sp. ISBSF 2019 e 2397 apresentaram atividade antibacteriana contra todas as bactérias-alvo testadas.(AU)
Descritores: Pseudomonas aeruginosa/crescimento & desenvolvimento
Staphylococcus aureus/crescimento & desenvolvimento
Streptomyces/metabolismo
Bacillus cereus/crescimento & desenvolvimento
Escherichia coli/crescimento & desenvolvimento
Antibacterianos/metabolismo
-Peptídeo Sintases/genética
Streptomyces/genética
Amplificação de Genes
Reação em Cadeia da Polimerase
Análise de Sequência de DNA
Primers do DNA
Policetídeo Sintases/genética
Antibacterianos/farmacologia
Responsável: BR1942.1 - NID - Biblioteca - Núcleo de Informação e Documentação


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Id: biblio-1057402
Autor: Alejo, Amanda; Burgueno, Eleuterio; Maldonado, Luis A; Herrera, Gabriel; Felix, Ruben; Quintana, Erika T.
Título: Efecto in vitro del extracto crudo de un estreptomiceto causante de sarna común de papa en Sinaloa, México / In vitro effect of the crude extract of a potato common scab streptomycete in Sinaloa, Mexico
Fonte: Rev. argent. microbiol;51(4):363-370, dic. 2019. graf.
Idioma: en.
Resumo: Abstract A strain isolated from potato common scab superficial lesions in El Fuerte Valley in northern Sinaloa, Mexico, was identified by 16S rRNA and morphological methods. Moreover, the effects of the crude extract of strain V2 was evaluated on radish and potato. The isolate was similar to Streptomyces acidiscabies in its morphological properties; however, the 16S rRNA gene sequence of strain V2 was neither 100% identical to this species nor to the streptomycetes previously reported in Sinaloa, Mexico. Strain V2 did not amplify any specific PCR products for genes necl and tomA, which have been found and reported in S. acidiscabies. Strain V2 produced a PCR product for the txtAB operon, which is related to the production of thaxtomin. In vitro assays using crude thaxtomin extract and a spore suspension of the organism caused necrotic symptoms on radish and potato, which were highly virulent in potato. This study reports that Streptomyces sp. V2 has a toxigenic region (TR) that is associated with the thaxtomin gene cluster.

Resumen Se aisló una cepa de una lesión superficial de sarna común de la papa en un ejemplar procedente del Valle del Fuerte, en el norte de Sinaloa, México. La cepa fue identificada por secuenciación del gen 16S ARNr, y por sus características morfológicas. Los efectos del extracto crudo de dicha cepa, llamada V2, fue evaluado en papa y rábano. El aislado fue similar a Streptomyces acidiscabies en sus características morfológicas, pero la secuencia del gen 16S ARNr de la cepa V2 no fue 100% idéntica a la de dicha especie, ni tampoco a las de cepas identificadas dentro de este taxón previamente en Sinaloa, México. La cepa V2 no amplificó los productos específicos de PCR de los genes nec1 y tomA, los cuales sí se han reportado en S. acidiscabies. La cepa V2 amplificó el producto de PCR para del operón txtAB, relacionado con la producción de taxtomina. A través de ensayos in vitro usando un extracto crudo de taxtomina y una suspensión de esporas del organismo aislado se verificó la producción de síntomas necróticos en rábano y papa, con mayor virulencia en esta última especie. Este estudio indica que Streptomyces sp. V2 tiene una región toxigénica (TR) asociada con el cluster de genes de taxtomina.
Descritores: Streptomyces/isolamento & purificação
Streptomyces/patogenicidade
Solanum tuberosum/microbiologia
-Técnicas In Vitro/métodos
Tipo de Publ: Ensaio Clínico
Estudo de Avaliação
Estudo Observacional
Responsável: AR635.1 - FCVyS - Servicio de Información y Documentación


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Id: biblio-1055109
Autor: García-Bernal, M; Medina-Marrero, R; Campa-Córdova, Á. I; Mazón-Suástegui, J. M.
Título: Growth and antioxidant response of juvenile oysters Crassostrea sikamea and Crassostrea corteziensis treated with Streptomyces strains / Crescimento e resposta oxidative de ostras jovens Crassostrea sikamea e Crassostrea corteziensis tratadas com cultura Streptomyces
Fonte: Arq. bras. med. vet. zootec. (Online);71(6):1993-1998, Nov.-Dec. 2019. tab, graf.
Idioma: en.
Projeto: Sectorial Fund for Education of Mexico.
Resumo: The effect of three Streptomyces strains (N7, RL8 and V4) and a mixture of Bacillus (BMix) on the growth (Weight, Size) and superoxide dismutase activity (SOD) in hatchery-reared juvenile oysters Crassostrea corteziensis and Crassostrea sikamea was investigated to determine their probiotic potential. Microorganisms were added to culture water at 1×106 CFU/ml once a day during 30 days and all oysters fed daily a microalgae mix. Juveniles of C. sikamea treated with strains N7, RL8 and V4 had a significant weight gain compared to the control group. C. corteziensis juveniles treated with strains RL8 and BMix showed a significantly higher weight gain than the control group. No significant size increase was observed in any treated group for both oyster species. SOD activity significantly increased in C. sikamea treated with RL8 and with RL8, N7 and BMix in C. corteziensis. Streptomyces strains RL8 and N7 emerge as promising probiotic agents to cultivate C. sikamea and C. corteziensis and may also be useful to other molluscs and marine invertebrates .(AU)

O efeito de três culturas Streptomyces (N7, RL8 e V4) e uma mistura de Bacillus (BMix) sobre o cresimento (Peso, Tamanho) e atividade superóxido dismutase (SOD) em ostras jovens Crassostrea corteziensis e Crassostrea sikamea cultivadas artificalmente foi investigado para determinar seu potencial probiótico. Microorganismos foram adicionados à água de cultura a 1×10 6 CFU/ml uma vez por dia durante 30 dias e todas as ostras foram alimentadas diariamente com uma mistura de microalgas. Jovens C. sikamea tratados com culturas N7, RL8 e V4 tiveram ganho de peso significativo quando comparado ao grupo de controle. Jovens C. corteziensis tratados com culturas RL8 e BMix demonstraram peso significativamente mais algo que o grupo de controle. Nenhum aumento em tamanho foi observado em grupos tratados em ambas espécies. A atividade SOD foi significamente aumentada em C. sikamea treatado com RL8 e com RL8, N7 e BMix em C. corteziensis. Culturas Streptomyces RL8 e N7 surgem como agentes probióticos promissores para o cultivo de C. sikamea e C. corteziensis e podem ser úteis para outros moluscos animais marinhos invertebrados.(AU)
Descritores: Streptomyces
Superóxido Dismutase/análise
Probióticos/administração & dosagem
Crassostrea/crescimento & desenvolvimento
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: biblio-839352
Autor: Dalitz, Camila de Araújo; Porsani, Mariana Vieira; Figel, Izabel Cristina; Pimentel, Ida C; Dalzoto, Patrícia R.
Título: Potential for biocontrol of melanized fungi by actinobacteria isolated from intertidal region of Ilha Do Mel, Paraná, Brazil
Fonte: Braz. j. microbiol;48(1):32-36, Jan.-Mar. 2017. tab, graf.
Idioma: en.
Resumo: Abstract Actinobacteria occur in many environments and have the capacity to produce secondary metabolites with antibiotic potential. Identification and taxonomy of actinobacteria that produce antimicrobial substances is essential for the screening of new compounds, and sequencing of the 16S region of ribosomal DNA (rDNA), which is conserved and present in all bacteria, is an important method of identification. Melanized fungi are free-living organisms, which can also be pathogens of clinical importance. This work aimed to evaluate growth inhibition of melanized fungi by actinobacteria and to identify the latter to the species level. In this study, antimicrobial activity of 13 actinobacterial isolates from the genus Streptomyces was evaluated against seven melanized fungi of the genera Exophiala, Cladosporium, and Rhinocladiella. In all tests, all actinobacterial isolates showed inhibitory activity against all isolates of melanized fungi, and only one actinobacterial isolate had less efficient inhibitory activity. The 16S rDNA region of five previously unidentified actinobacterial isolates from Ilha do Mel, Paraná, Brazil, was sequenced; four of the isolates were identified as Streptomyces globisporus subsp. globisporus, and one isolate was identified as Streptomyces aureus. This work highlights the potential of actinobacteria with antifungal activity and their role in the pursuit of novel antimicrobial substances.
Descritores: Actinobacteria/fisiologia
Fungos/fisiologia
Antibiose
-Filogenia
Streptomyces/classificação
Streptomyces/genética
Brasil
RNA Ribossômico 16S/genética
Actinobacteria/isolamento & purificação
Actinobacteria/classificação
Actinobacteria/genética
Responsável: BR1.1 - BIREME


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Id: biblio-974313
Autor: Rodrigues, Kaio César da Silva; Souza, Arianne Tairyne de; Badino, Alberto Colli; Pedrolli, Danielle Biscaro; Cerri, Marcel Otavio.
Título: Screening of medium constituents for clavulanic acid production by Streptomyces clavuligerus
Fonte: Braz. j. microbiol;49(4):832-839, Oct.-Dec. 2018. tab, graf.
Idioma: en.
Projeto: CNPQ.
Resumo: ABSTRACT Clavulanic acid is a β-lactam compound with potent inhibitory activity against β-lactamases. Studies have shown that certain amino acids play essential roles in CA biosynthesis. However, quantitative evaluations of the effects of these amino acids are still needed in order to improve CA production. Here, we report a study of the nutritional requirements of Streptomyces clavuligerus for CA production. Firstly, the influence of the primary nitrogen source and the salts composition was investigated. Subsequently, soybean protein isolate was supplemented with arginine (0.0-3.20 g L-1), threonine (0.0-1.44 g L-1), ornithine (0.0-4.08 g L-1), and glutamate (0.0-8.16 g L-1), according to a two-level central composite rotatable design. A medium containing ferrous sulfate yielded CA production of 437 mg L-1, while a formulation without this salt produced only 41 mg L-1 of CA. This substantial difference suggested that Fe2+ is important for CA biosynthesis. The experimental design showed that glutamate and ornithine negatively influenced CA production while arginine and threonine had no influence. The soybean protein isolate provided sufficient C5 precursor for CA biosynthesis, so that supplementation was unnecessary. Screening of medium components, together with experimental design tools, could be a valuable way of enhancing CA titers and reducing the process costs.
Descritores: Streptomyces/metabolismo
Ácido Clavulânico/biossíntese
Meios de Cultura/metabolismo
-Ornitina/análise
Ornitina/metabolismo
Streptomyces/genética
Ácido Glutâmico/análise
Ácido Glutâmico/metabolismo
Meios de Cultura/química
Nitrogênio/análise
Nitrogênio/metabolismo
Responsável: BR1.1 - BIREME


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Id: biblio-974291
Autor: Agunbiade, Mayowa; Pohl, Carolina; Ashafa, Omotayo.
Título: Bioflocculant production from Streptomyces platensis and its potential for river and waste water treatment
Fonte: Braz. j. microbiol;49(4):731-741, Oct.-Dec. 2018. tab, graf.
Idioma: en.
Resumo: ABSTRACT A bacterium isolated from Sterkfontein dam was confirmed to produce bioflocculant with excellent flocculation activity. The 16S rDNA nucleotide sequence analyses revealed the bacteria to have 99% similarity to Streptomyces platensis strain HBUM174787 and the sequence was deposited in the Genbank as Streptomyces platensis with accession number FJ 486385.1. Culture conditions for optimal production of the bioflocculant included glucose as a sole carbon source, resulting in flocculating activity of 90%. Other optimal conditions included: peptone as nitrogen source; presence of Mg2+ as cations and inoculum size of 1.0% (v/v) at neutral pH of 7. Optimum dose of the purified bioflocculant for the clarification of 4 g/L kaolin clay suspension at neutral pH was 0.2 mg/mL. Energy Dispersive X-ray analysis confirmed elemental composition of the purified bioflocculant in mass proportion (%w/w): carbon (21.41), oxygen (35.59), sulphur (26.16), nitrogen (0.62) and potassium (7.48). Fourier Transform Infrared Spectroscopy (FTIR) indicated the presence of hydroxyl, carboxyl, methoxyl and amino group in the bioflocculant. The bioflocculant produced by S. platensis removed chemical oxygen demand (COD) in river water and meat processing wastewater at efficiencies of 63.1 and 46.6% respectively and reduced their turbidity by 84.3 and 75.6% respectively. The high flocculating rate and removal efficiencies displayed by S. platensis suggests its industrial application in wastewater treatment.
Descritores: Streptomyces/química
Proteínas de Bactérias/metabolismo
Águas Residuárias/química
-Streptomyces/isolamento & purificação
Streptomyces/genética
Streptomyces/metabolismo
Proteínas de Bactérias/genética
Microbiologia da Água
Carbono/metabolismo
Purificação da Água
Rios/química
Floculação
Nitrogênio/metabolismo
Responsável: BR1.1 - BIREME


  7 / 90 LILACS  
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Id: biblio-974289
Autor: Charousová, Ivana; Medo, Juraj; Hleba, Lukáš; Javoreková, Soňa.
Título: Streptomyces globosus DK15 and Streptomyces ederensis ST13 as new producers of factumycin and tetrangomycin antibiotics
Fonte: Braz. j. microbiol;49(4):816-822, Oct.-Dec. 2018. tab, graf.
Idioma: en.
Projeto: Slovak Academy of Sciences.
Resumo: ABSTRACT Fifty seven soil-borne actinomycete strains were assessed for the antibiotic production. Two of the most active isolates, designed as Streptomyces ST-13 and DK-15 exhibited a broad range of antimicrobial activity and therefore they were selected for HPLC fractionation against the most suppressed bacteria Staphylococcus aureus (ST-13) and Chromobacterium violaceum (DK-15). LC/MS analysis of extracts showed the presence of polyketides factumycin (DK15) and tetrangomycin (ST13). The taxonomic position of the antibiotic-producing actinomycetes was determined using a polyphasic approach. Phenotypic characterization and 16S rRNA gene sequence analysis of the isolates matched those described for members of the genus Streptomyces. DK-15 strain exhibited the highest 16S rRNA gene sequence similarity to Streptomyces globosus DSM-40815 (T) and Streptomyces toxytricini DSM-40178 (T) and ST-13 strain to Streptomyces ederensis DSM-40741 (T) and Streptomyces phaeochromogenes DSM-40073 (T). For the proper identification, MALDI-TOF/MS profile of whole-cell proteins led to the identification of S. globosus DK-15 (accession number: KX527570) and S. ederensis ST13 (accession number: KX527568). To our knowledge, there is no report about the production of these antibiotics by S.globosus and S. ederensis, thus isolates DK15 and ST13 identified as S. globosus DK-15 and S.ederensis ST-13 can be considered as new sources of these unique antibacterial metabolites.
Descritores: Streptomyces/isolamento & purificação
Streptomyces/metabolismo
Antibacterianos/biossíntese
-Filogenia
Piridonas/metabolismo
Microbiologia do Solo
Streptomyces/classificação
Streptomyces/genética
Benzo(a)Antracenos/metabolismo
DNA Bacteriano/genética
Técnicas de Tipagem Bacteriana
Responsável: BR1.1 - BIREME


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Id: lil-788982
Autor: Rosa, Juliana Pacheco da; Tibúrcio, Samyra Raquel Gonçalves; Marques, Joana Montezano; Seldin, Lucy; Coelho, Rosalie Reed Rodrigues.
Título: Streptomyces lunalinharesii 235 prevents the formation of a sulfate-reducing bacterial biofilm
Fonte: Braz. j. microbiol;47(3):603-609, July-Sept. 2016. graf.
Idioma: en.
Resumo: ABSTRACT Streptomyces lunalinharesii strain 235 produces an antimicrobial substance that is active against sulfate reducing bacteria, the major bacterial group responsible for biofilm formation and biocorrosion in petroleum reservoirs. The use of this antimicrobial substance for sulfate reducing bacteria control is therefore a promising alternative to chemical biocides. In this study the antimicrobial substance did not interfere with the biofilm stability, but the sulfate reducing bacteria biofilm formation was six-fold smaller in carbon steel coupons treated with the antimicrobial substance when compared to the untreated control. A reduction in the most probable number counts of planktonic cells of sulfate reducing bacteria was observed after treatments with the sub-minimal inhibitory concentration, minimal inhibitory concentration, and supra-minimal inhibitory concentration of the antimicrobial substance. Additionally, when the treated coupons were analyzed by scanning electron microscopy, the biofilm formation was found to be substantially reduced when the supra-minimal inhibitory concentration of the antimicrobial substance was used. The coupons used for the biofilm formation had a small weight loss after antimicrobial substance treatment, but corrosion damage was not observed by scanning electron microscopy. The absence of the dsrA gene fragment in the scraped cell suspension after treatment with the supra-minimal inhibitory concentration of the antimicrobial substance suggests that Desulfovibrio alaskensis was not able to adhere to the coupons. This is the first report on an antimicrobial substance produced by Streptomyces active against sulfate reducing bacteria biofilm formation. The application of antimicrobial substance as a potential biocide for sulfate reducing bacteria growth control could be of great interest to the petroleum industry.
Descritores: Oxirredução
Streptomyces/fisiologia
Sulfatos/metabolismo
Biofilmes
Antibiose
-Streptomyces/efeitos dos fármacos
Streptomyces/ultraestrutura
Testes de Sensibilidade Microbiana
Biofilmes/crescimento & desenvolvimento
Biofilmes/efeitos dos fármacos
Antibacterianos/farmacologia
Responsável: BR1.1 - BIREME


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Id: biblio-1048701
Autor: Zheng, Xiong; Ye, Ruifang; Hu, Fengxian; Mao, Quangui; Zhang, Hongzhou.
Título: Effects of precursors on kitasamycin production in streptomyces kitasatoensis / Efeitos dos precursores na produção de kitasamicina em Streptomyces kitasatoensis
Fonte: Biosci. j. (Online);35(3):910-919, may./jun. 2019. tab, graf.
Idioma: en.
Resumo: To improve kitasamycin biosynthesis by Streptomyces kitasatoensis Z-7, the addition of two precursors, sodium acetate and ethyl acetate, to the fermentation medium was evaluated. Ethyl acetate was the most effective precursor compared with control conditions; In a 15-L fermentor, the kitasamycin titer was 21% higher when 0.48% ethyl acetate was added compared to control conditions. Content of the A5 component increased by 5.1%, and the A4 content decreased slightly compared to that of the control. During kitasamycin synthesis, intracellular and extracellular concentrations of acetic acid were higher for S. kitasatoensis Z-7 supplemented with ethyl acetate than for the non-supplemented strain, and the activities of acyl-CoA synthetases, acyl-phosphotransferases, and acyl-kinases were also significantly increased, suggesting that increased acetyl-CoA levels can explain the high kitasamycin titer. These findings may improve the industrial-scale production of kitasamycin for clinical use, and the addition of 0.48% ethyl acetate as precursors in the medium at the beginning of cultivation was a new method to mitigate the negative influence on the cell growth of excess precursor.

Para melhorar a biossíntese de kitasamicina por Streptomyces kitasatoensis Z-7, a adição de dois precursores, acetato de sódio e acetato de etila, ao meio de fermentação foi avaliada. O acetato de etila foi o precursor mais efetivo em comparação com as condições de controle; Em um fermentador de 15 L, o título de kitasamicina foi 21% maior quando 0,48% de acetato de etila foi adicionado em comparação com as condições de controle. O conteúdo do componente A5 aumentou 5,1%, e o conteúdo A4 diminuiu ligeiramente em comparação com o do controle. Durante a síntese de kitasamicina, as concentrações intracelulares e extracelulares de ácido acético foram maiores para S. kitasatoensis Z-7 suplementado com acetato de etila do que para a cepa não suplementada, e as atividades de acil-CoA sintetases, acil-fosfotransferases e acil-cinases também foram significativamente aumentadas, sugerindo que níveis aumentados de acetil-CoA podem explicar o alto título de kitasamicina. Esses achados podem melhorar a produção em escala industrial da kitasamicina para uso clínico, e a adição de 0,48% de acetato de etila como precursores no meio no início do cultivo foi um novo método para mitigar a influência negativa no crescimento celular do excesso de precursor.
Descritores: Streptomyces
Kitasamicina
Fermentação
Antibacterianos
Responsável: BR396.1 - Biblioteca Central


  10 / 90 LILACS  
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Id: biblio-1015839
Autor: Qin, Ronghuo; Zhong, Chuanqing; Zong, Gongli; Fu, Jiafang; Pang, Xiuhua; Cao, Guangxiang.
Título: Improvement of clavulanic acid production in Streptomyces clavuligerus F613-1 by using a claR-neo reporter strategy
Fonte: Electron. j. biotechnol;28:41-46, July. 2017. tab, ilus, graf.
Idioma: en.
Projeto: National Natural Science Foundation; . Shandong Natural Science Foundation; . Innovation Project of Shandong Academy of Medical Sciences.
Resumo: Background: Streptomyces clavuligerus was the producer of clavulanic acid, claR, a pathway-specific transcriptional regulator in S. clavuligerus, positively regulates clavulanic acid biosynthesis. In this study, the promoter-less kanamycin resistance gene neo was fused with claR to obtain strain NEO from S. clavuligerus F613-1. The claR-neo fusion strain NEO was mutated using physical and chemical mutagens and then screened under high concentrations of kanamycin for high-yield producers of clavulanic acid. Results: The reporter gene neo was fused downstream of claR and used as an indicator for expression levels of claR in strain NEO. After three rounds of continuous treatment and screening, the high-yield clavulanic acid-producing strain M3-19 was obtained. In the shaking flask model, the clavulanic acid titer of M3-19 reached 4.33 g/L, which is an increase of 33% over the titer of 3.26 g/L for the starting strains S. clavuligerus F613-1 and NEO. Conclusions: Our results indicate that neo can be effectively used as a reporter for the expression of late-stage biosynthetic genes when screening for high-yield strains and that this approach has strong potential for improving Streptomyces strains of industrial value.
Descritores: Streptomyces/genética
Streptomyces/metabolismo
Canamicina
Ácido Clavulânico/biossíntese
-Fatores de Transcrição/genética
Transcrição Genética
Bioensaio
Proteínas Recombinantes
Cromatografia Líquida de Alta Pressão
Mutagênese
Regiões Promotoras Genéticas
Genes Reporter
Fusão Gênica
Fermentação
Reação em Cadeia da Polimerase em Tempo Real
Responsável: CL1.1 - Biblioteca Central



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