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Id: biblio-839352
Autor: Dalitz, Camila de Araújo; Porsani, Mariana Vieira; Figel, Izabel Cristina; Pimentel, Ida C; Dalzoto, Patrícia R.
Título: Potential for biocontrol of melanized fungi by actinobacteria isolated from intertidal region of Ilha Do Mel, Paraná, Brazil
Fonte: Braz. j. microbiol;48(1):32-36, Jan.-Mar. 2017. tab, graf.
Idioma: en.
Resumo: Abstract Actinobacteria occur in many environments and have the capacity to produce secondary metabolites with antibiotic potential. Identification and taxonomy of actinobacteria that produce antimicrobial substances is essential for the screening of new compounds, and sequencing of the 16S region of ribosomal DNA (rDNA), which is conserved and present in all bacteria, is an important method of identification. Melanized fungi are free-living organisms, which can also be pathogens of clinical importance. This work aimed to evaluate growth inhibition of melanized fungi by actinobacteria and to identify the latter to the species level. In this study, antimicrobial activity of 13 actinobacterial isolates from the genus Streptomyces was evaluated against seven melanized fungi of the genera Exophiala, Cladosporium, and Rhinocladiella. In all tests, all actinobacterial isolates showed inhibitory activity against all isolates of melanized fungi, and only one actinobacterial isolate had less efficient inhibitory activity. The 16S rDNA region of five previously unidentified actinobacterial isolates from Ilha do Mel, Paraná, Brazil, was sequenced; four of the isolates were identified as Streptomyces globisporus subsp. globisporus, and one isolate was identified as Streptomyces aureus. This work highlights the potential of actinobacteria with antifungal activity and their role in the pursuit of novel antimicrobial substances.
Descritores: Actinobacteria/fisiologia
Fungos/fisiologia
Antibiose
-Filogenia
Streptomyces/classificação
Streptomyces/genética
Brasil
RNA Ribossômico 16S/genética
Actinobacteria/isolamento & purificação
Actinobacteria/classificação
Actinobacteria/genética
Responsável: BR1.1 - BIREME


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Id: biblio-974313
Autor: Rodrigues, Kaio César da Silva; Souza, Arianne Tairyne de; Badino, Alberto Colli; Pedrolli, Danielle Biscaro; Cerri, Marcel Otavio.
Título: Screening of medium constituents for clavulanic acid production by Streptomyces clavuligerus
Fonte: Braz. j. microbiol;49(4):832-839, Oct.-Dec. 2018. tab, graf.
Idioma: en.
Projeto: CNPQ.
Resumo: ABSTRACT Clavulanic acid is a β-lactam compound with potent inhibitory activity against β-lactamases. Studies have shown that certain amino acids play essential roles in CA biosynthesis. However, quantitative evaluations of the effects of these amino acids are still needed in order to improve CA production. Here, we report a study of the nutritional requirements of Streptomyces clavuligerus for CA production. Firstly, the influence of the primary nitrogen source and the salts composition was investigated. Subsequently, soybean protein isolate was supplemented with arginine (0.0-3.20 g L-1), threonine (0.0-1.44 g L-1), ornithine (0.0-4.08 g L-1), and glutamate (0.0-8.16 g L-1), according to a two-level central composite rotatable design. A medium containing ferrous sulfate yielded CA production of 437 mg L-1, while a formulation without this salt produced only 41 mg L-1 of CA. This substantial difference suggested that Fe2+ is important for CA biosynthesis. The experimental design showed that glutamate and ornithine negatively influenced CA production while arginine and threonine had no influence. The soybean protein isolate provided sufficient C5 precursor for CA biosynthesis, so that supplementation was unnecessary. Screening of medium components, together with experimental design tools, could be a valuable way of enhancing CA titers and reducing the process costs.
Descritores: Streptomyces/metabolismo
Ácido Clavulânico/biossíntese
Meios de Cultura/metabolismo
-Ornitina/análise
Ornitina/metabolismo
Streptomyces/genética
Ácido Glutâmico/análise
Ácido Glutâmico/metabolismo
Meios de Cultura/química
Nitrogênio/análise
Nitrogênio/metabolismo
Responsável: BR1.1 - BIREME


  3 / 87 LILACS  
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Id: biblio-974291
Autor: Agunbiade, Mayowa; Pohl, Carolina; Ashafa, Omotayo.
Título: Bioflocculant production from Streptomyces platensis and its potential for river and waste water treatment
Fonte: Braz. j. microbiol;49(4):731-741, Oct.-Dec. 2018. tab, graf.
Idioma: en.
Resumo: ABSTRACT A bacterium isolated from Sterkfontein dam was confirmed to produce bioflocculant with excellent flocculation activity. The 16S rDNA nucleotide sequence analyses revealed the bacteria to have 99% similarity to Streptomyces platensis strain HBUM174787 and the sequence was deposited in the Genbank as Streptomyces platensis with accession number FJ 486385.1. Culture conditions for optimal production of the bioflocculant included glucose as a sole carbon source, resulting in flocculating activity of 90%. Other optimal conditions included: peptone as nitrogen source; presence of Mg2+ as cations and inoculum size of 1.0% (v/v) at neutral pH of 7. Optimum dose of the purified bioflocculant for the clarification of 4 g/L kaolin clay suspension at neutral pH was 0.2 mg/mL. Energy Dispersive X-ray analysis confirmed elemental composition of the purified bioflocculant in mass proportion (%w/w): carbon (21.41), oxygen (35.59), sulphur (26.16), nitrogen (0.62) and potassium (7.48). Fourier Transform Infrared Spectroscopy (FTIR) indicated the presence of hydroxyl, carboxyl, methoxyl and amino group in the bioflocculant. The bioflocculant produced by S. platensis removed chemical oxygen demand (COD) in river water and meat processing wastewater at efficiencies of 63.1 and 46.6% respectively and reduced their turbidity by 84.3 and 75.6% respectively. The high flocculating rate and removal efficiencies displayed by S. platensis suggests its industrial application in wastewater treatment.
Descritores: Streptomyces/química
Proteínas de Bactérias/metabolismo
Águas Residuais/química
-Streptomyces/isolamento & purificação
Streptomyces/genética
Streptomyces/metabolismo
Proteínas de Bactérias/genética
Microbiologia da Água
Carbono/metabolismo
Purificação da Água
Rios/química
Floculação
Nitrogênio/metabolismo
Responsável: BR1.1 - BIREME


  4 / 87 LILACS  
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Id: biblio-974289
Autor: Charousová, Ivana; Medo, Juraj; Hleba, Lukáš; Javoreková, Soňa.
Título: Streptomyces globosus DK15 and Streptomyces ederensis ST13 as new producers of factumycin and tetrangomycin antibiotics
Fonte: Braz. j. microbiol;49(4):816-822, Oct.-Dec. 2018. tab, graf.
Idioma: en.
Projeto: Slovak Academy of Sciences.
Resumo: ABSTRACT Fifty seven soil-borne actinomycete strains were assessed for the antibiotic production. Two of the most active isolates, designed as Streptomyces ST-13 and DK-15 exhibited a broad range of antimicrobial activity and therefore they were selected for HPLC fractionation against the most suppressed bacteria Staphylococcus aureus (ST-13) and Chromobacterium violaceum (DK-15). LC/MS analysis of extracts showed the presence of polyketides factumycin (DK15) and tetrangomycin (ST13). The taxonomic position of the antibiotic-producing actinomycetes was determined using a polyphasic approach. Phenotypic characterization and 16S rRNA gene sequence analysis of the isolates matched those described for members of the genus Streptomyces. DK-15 strain exhibited the highest 16S rRNA gene sequence similarity to Streptomyces globosus DSM-40815 (T) and Streptomyces toxytricini DSM-40178 (T) and ST-13 strain to Streptomyces ederensis DSM-40741 (T) and Streptomyces phaeochromogenes DSM-40073 (T). For the proper identification, MALDI-TOF/MS profile of whole-cell proteins led to the identification of S. globosus DK-15 (accession number: KX527570) and S. ederensis ST13 (accession number: KX527568). To our knowledge, there is no report about the production of these antibiotics by S.globosus and S. ederensis, thus isolates DK15 and ST13 identified as S. globosus DK-15 and S.ederensis ST-13 can be considered as new sources of these unique antibacterial metabolites.
Descritores: Streptomyces/isolamento & purificação
Streptomyces/metabolismo
Antibacterianos/biossíntese
-Filogenia
Piridonas/metabolismo
Microbiologia do Solo
Streptomyces/classificação
Streptomyces/genética
Benzo(a)Antracenos/metabolismo
DNA Bacteriano/genética
Técnicas de Tipagem Bacteriana
Responsável: BR1.1 - BIREME


  5 / 87 LILACS  
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Id: lil-788982
Autor: Rosa, Juliana Pacheco da; Tibúrcio, Samyra Raquel Gonçalves; Marques, Joana Montezano; Seldin, Lucy; Coelho, Rosalie Reed Rodrigues.
Título: Streptomyces lunalinharesii 235 prevents the formation of a sulfate-reducing bacterial biofilm
Fonte: Braz. j. microbiol;47(3):603-609, July-Sept. 2016. graf.
Idioma: en.
Resumo: ABSTRACT Streptomyces lunalinharesii strain 235 produces an antimicrobial substance that is active against sulfate reducing bacteria, the major bacterial group responsible for biofilm formation and biocorrosion in petroleum reservoirs. The use of this antimicrobial substance for sulfate reducing bacteria control is therefore a promising alternative to chemical biocides. In this study the antimicrobial substance did not interfere with the biofilm stability, but the sulfate reducing bacteria biofilm formation was six-fold smaller in carbon steel coupons treated with the antimicrobial substance when compared to the untreated control. A reduction in the most probable number counts of planktonic cells of sulfate reducing bacteria was observed after treatments with the sub-minimal inhibitory concentration, minimal inhibitory concentration, and supra-minimal inhibitory concentration of the antimicrobial substance. Additionally, when the treated coupons were analyzed by scanning electron microscopy, the biofilm formation was found to be substantially reduced when the supra-minimal inhibitory concentration of the antimicrobial substance was used. The coupons used for the biofilm formation had a small weight loss after antimicrobial substance treatment, but corrosion damage was not observed by scanning electron microscopy. The absence of the dsrA gene fragment in the scraped cell suspension after treatment with the supra-minimal inhibitory concentration of the antimicrobial substance suggests that Desulfovibrio alaskensis was not able to adhere to the coupons. This is the first report on an antimicrobial substance produced by Streptomyces active against sulfate reducing bacteria biofilm formation. The application of antimicrobial substance as a potential biocide for sulfate reducing bacteria growth control could be of great interest to the petroleum industry.
Descritores: Oxirredução
Streptomyces/fisiologia
Sulfatos/metabolismo
Biofilmes
Antibiose
-Streptomyces/efeitos dos fármacos
Streptomyces/ultraestrutura
Testes de Sensibilidade Microbiana
Biofilmes/crescimento & desenvolvimento
Biofilmes/efeitos dos fármacos
Antibacterianos/farmacologia
Responsável: BR1.1 - BIREME


  6 / 87 LILACS  
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Id: biblio-1048701
Autor: Zheng, Xiong; Ye, Ruifang; Hu, Fengxian; Mao, Quangui; Zhang, Hongzhou.
Título: Effects of precursors on kitasamycin production in streptomyces kitasatoensis / Efeitos dos precursores na produção de kitasamicina em Streptomyces kitasatoensis
Fonte: Biosci. j. (Online);35(3):910-919, may./jun. 2019. tab, graf.
Idioma: en.
Resumo: To improve kitasamycin biosynthesis by Streptomyces kitasatoensis Z-7, the addition of two precursors, sodium acetate and ethyl acetate, to the fermentation medium was evaluated. Ethyl acetate was the most effective precursor compared with control conditions; In a 15-L fermentor, the kitasamycin titer was 21% higher when 0.48% ethyl acetate was added compared to control conditions. Content of the A5 component increased by 5.1%, and the A4 content decreased slightly compared to that of the control. During kitasamycin synthesis, intracellular and extracellular concentrations of acetic acid were higher for S. kitasatoensis Z-7 supplemented with ethyl acetate than for the non-supplemented strain, and the activities of acyl-CoA synthetases, acyl-phosphotransferases, and acyl-kinases were also significantly increased, suggesting that increased acetyl-CoA levels can explain the high kitasamycin titer. These findings may improve the industrial-scale production of kitasamycin for clinical use, and the addition of 0.48% ethyl acetate as precursors in the medium at the beginning of cultivation was a new method to mitigate the negative influence on the cell growth of excess precursor.

Para melhorar a biossíntese de kitasamicina por Streptomyces kitasatoensis Z-7, a adição de dois precursores, acetato de sódio e acetato de etila, ao meio de fermentação foi avaliada. O acetato de etila foi o precursor mais efetivo em comparação com as condições de controle; Em um fermentador de 15 L, o título de kitasamicina foi 21% maior quando 0,48% de acetato de etila foi adicionado em comparação com as condições de controle. O conteúdo do componente A5 aumentou 5,1%, e o conteúdo A4 diminuiu ligeiramente em comparação com o do controle. Durante a síntese de kitasamicina, as concentrações intracelulares e extracelulares de ácido acético foram maiores para S. kitasatoensis Z-7 suplementado com acetato de etila do que para a cepa não suplementada, e as atividades de acil-CoA sintetases, acil-fosfotransferases e acil-cinases também foram significativamente aumentadas, sugerindo que níveis aumentados de acetil-CoA podem explicar o alto título de kitasamicina. Esses achados podem melhorar a produção em escala industrial da kitasamicina para uso clínico, e a adição de 0,48% de acetato de etila como precursores no meio no início do cultivo foi um novo método para mitigar a influência negativa no crescimento celular do excesso de precursor.
Descritores: Streptomyces
Kitasamicina
Fermentação
Antibacterianos
Responsável: BR396.1 - Biblioteca Central


  7 / 87 LILACS  
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Id: biblio-1015839
Autor: Qin, Ronghuo; Zhong, Chuanqing; Zong, Gongli; Fu, Jiafang; Pang, Xiuhua; Cao, Guangxiang.
Título: Improvement of clavulanic acid production in Streptomyces clavuligerus F613-1 by using a claR-neo reporter strategy
Fonte: Electron. j. biotechnol;28:41-46, July. 2017. tab, ilus, graf.
Idioma: en.
Projeto: National Natural Science Foundation; . Shandong Natural Science Foundation; . Innovation Project of Shandong Academy of Medical Sciences.
Resumo: Background: Streptomyces clavuligerus was the producer of clavulanic acid, claR, a pathway-specific transcriptional regulator in S. clavuligerus, positively regulates clavulanic acid biosynthesis. In this study, the promoter-less kanamycin resistance gene neo was fused with claR to obtain strain NEO from S. clavuligerus F613-1. The claR-neo fusion strain NEO was mutated using physical and chemical mutagens and then screened under high concentrations of kanamycin for high-yield producers of clavulanic acid. Results: The reporter gene neo was fused downstream of claR and used as an indicator for expression levels of claR in strain NEO. After three rounds of continuous treatment and screening, the high-yield clavulanic acid-producing strain M3-19 was obtained. In the shaking flask model, the clavulanic acid titer of M3-19 reached 4.33 g/L, which is an increase of 33% over the titer of 3.26 g/L for the starting strains S. clavuligerus F613-1 and NEO. Conclusions: Our results indicate that neo can be effectively used as a reporter for the expression of late-stage biosynthetic genes when screening for high-yield strains and that this approach has strong potential for improving Streptomyces strains of industrial value.
Descritores: Streptomyces/genética
Streptomyces/metabolismo
Canamicina
Ácido Clavulânico/biossíntese
-Fatores de Transcrição/genética
Transcrição Genética
Bioensaio
Proteínas Recombinantes
Cromatografia Líquida de Alta Pressão
Mutagênese
Regiões Promotoras Genéticas
Genes Reporter
Fusão Gênica
Fermentação
Reação em Cadeia da Polimerase em Tempo Real
Responsável: CL1.1 - Biblioteca Central


  8 / 87 LILACS  
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Id: biblio-889240
Autor: Ser, Hooi-Leng; Tan, Wen-Si; Mutalib, Nurul-Syakima Ab; Yin, Wai-Fong; Chan, Kok-Gan; Goh, Bey-Hing; Lee, Learn-Han.
Título: Genome sequence of Streptomyces gilvigriseus MUSC 26T isolated from mangrove forest
Fonte: Braz. j. microbiol;49(2):207-209, Apr.-June 2018. tab.
Idioma: en.
Projeto: PVC Award Grant; . Biotek Abadi; . MOSTI eScience funds; . UM-MOHE HIR Nature Microbiome.
Resumo: Abstract Streptomycetes remain as one of the important sources for bioactive products. Isolated from the mangrove forest, Streptomyces gilvigriseus MUSC 26T was previously characterised as a novel streptomycete. The high quality draft genome of MUSC 26T contained 5,213,277 bp with G + C content of 73.0%. Through genome mining, several gene clusters associated with secondary metabolites production were revealed in the genome of MUSC 26T. These findings call for further investigations into the potential exploitation of the strain for production of pharmaceutically important compounds.
Descritores: Streptomyces/genética
Genoma Bacteriano
Microbiologia Ambiental
-Streptomyces/isolamento & purificação
Composição de Bases
Produtos Biológicos/metabolismo
Análise de Sequência de DNA
Biologia Computacional
Zonas Úmidas
Redes e Vias Metabólicas/genética
Metabolismo Secundário
Responsável: BR1.1 - BIREME


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Id: biblio-889194
Autor: Ser, Hooi-Leng; Tan, Wen-Si; Ab Mutalib, Nurul-Syakima; Yin, Wai-Fong; Chan, Kok-Gan; Goh, Bey-Hing; Lee, Learn-Han.
Título: Genome sequence of Streptomyces mangrovisoli MUSC 149T isolated from intertidal sediments
Fonte: Braz. j. microbiol;49(1):13-15, Jan.-Mar. 2018. tab, graf.
Idioma: en.
Projeto: PVC; . External Industry; . Fundamental Research Grant Scheme; . eScience; . University of Malaya; . PPP.
Resumo: ABSTRACT As the largest genus in Actinobacteria family, Streptomyces species have the ability to synthesize numerous compounds of diverse structures with bioactivities. Streptomyces mangrovisoli MUSC 149T was previously isolated as a novel streptomycete from mangrove forest in east coast of Peninsular Malaysia. The high quality draft genome of MUSC 149T comprises 9,165,825 bp with G + C content of 72.5%. Through bioinformatics analysis, 21 gene clusters identified in the genome were associated with the production of bioactive secondary metabolites. The presence of these biosynthetic gene clusters in MUSC 149T suggests the potential exploitation of the strain for production of medically important compounds.
Descritores: Streptomyces/isolamento & purificação
Genoma Bacteriano
Sedimentos Geológicos/microbiologia
-Filogenia
Streptomyces/classificação
Streptomyces/genética
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Composição de Bases
DNA Bacteriano/genética
Dados de Sequência Molecular
Sequência de Bases
Malásia
Responsável: BR1.1 - BIREME


  10 / 87 LILACS  
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Id: biblio-965726
Autor: Naine, S Jemimah; Devi, C Subathra; Mohanasrinivasan, V.
Título: In vitro thrombolytic potential of bioactive compounds from marine Streptomyces sp. VITJS4 / Potencial trombolítico in vitro de compostos bioativos de Streptomyces marinhos sp. VITJS4
Fonte: Biosci. j. (Online);32(5):1314-1323, sept./oct 2016. ilus, tab, graf.
Idioma: en.
Resumo: The most practical approach to reduce morbidity and mortality of coronary heart disease (CHD) is to delay the process of thrombus by usage of clot-dissolving agents. The necessities of such safer compounds are to be critically examined for thrombolytic activity especially, from marine sources. Thrombolytic agents have been investigated as a possible treatment for thrombus. The aim of this study was to investigate the in vitro thrombolytic potential of Streptomyces sp.VITJS4 (NCIM No. 5574); (ACC No: JQ234978.1) active compounds. The fibrin degradation revealed a clear transparent zone of clearance with 500µg/mL concentration showing 24mm hydrolysis. The thrombolytic effect of Streptomyces sp.VITJS4 compounds was also demonstrated in vitro clot lysis assay where The percent of thrombolysis by the crude extract showed 90±1.7% at the concentration of 1000µg/mL, whereas percent of thrombolysis by streptokinase was found 100± 00%%. The bioactive compounds were further studied for spectrophotometric analysis. The UV-VIS profile showed different peaks ranging from 400-700 nm with different absorption respectively. The data confirmed the presence of both analogues with absorption maxima at 210 and 310 nm. A sensitive method using LC-MS technique was optimized for the separation and identification of bioactive metabolites which was indicated by the fingerprints. The results of the LC-MS analysis provided different peaks determining the presence of compounds with different therapeutic activities. The current study refers the bioactive compound as impressive thrombolytic agent for further laboratory study. Further studies should be conducted to ensure the efficacy and safety of different concentration of bioactive compounds for drug development. Hence the results reported perhaps useful for the discovery of novel thrombolytic drugs from marine origin.

A abordagem mais prática para reduzir a morbidade e a mortalidade da doença arterial coronariana (CHD, do inglês coronary heart disease) consiste em retardar o processo de trombo através da utilização de agentes de dissolução de coágulos. As necessidades de tais compostos mais seguros devem ser criticamente examinadas para a atividade trombolítica, especialmente de fontes marinhas. Agentes trombolíticos tem sido estudados como um possível tratamento para o trombo. O objetivo deste estudo foi investigar o potencial trombolítico in vitro dos compostos ativos do Streptomyces sp.VITJS4 (NCIM No. 5574); (ACC No: JQ234978.1). A degradação da fibrina revelou um clara zona livre transparente com concentração de 500µg/mL mostrando uma hidrólise de 24mm. O efeito trombolítico dos compostos de Streptomyces sp.VITJS4 também foi demonstrado no ensaio in vitro de lise dos coágulos em que a percentagem de trombólise pelo extrato bruto mostrou 90±1.7% a uma concentração de 1000µg/mL, enquanto que a percentagem de trombólise pela estreptoquinase foi de 100± 00%. Os compostos bioativos foram estudados posteriormente através da análise espectrofotométrica. O perfil ultra violeta visível (UV-VIS profile, em inglês) mostrou diferentes picos variando entre 400-700 nm com diferentes absorções respectivamente. Os dados confirmaram a presença de ambos os análogos com absorção máxima em 210 e 300 nm. Um método sensível usando a técnica LC-MS (Liquid chromatography­mass spectrometry) foi otimizado para a separação e identificação metabólitos bioativos que foram indicados pelas impressões digitais (?). Os resultados da análise LC-MS forneceram diferentes picos determinando a presença de compostos com diferentes atividades terapêuticas. O estudo atual refere-se ao composto bioativo como um agente trombolítico impressionante para futuros estudos em laboratório. Estudos futuros devem ser conduzidos para assegurar a eficácia e segurança de diferentes concentrações dos compostos bioativos para o desenvolvimento de drogas. Assim, os resultados reportados talvez sejam úteis para a descoberta de novas drogas trombolíticas de origem marinha.
Descritores: Streptomyces
Trombose
Técnicas In Vitro
Actinobacteria
Fibrinolíticos
Responsável: BR396.1 - Biblioteca Central



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