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Id: biblio-1087172
Autor: Salam, Menaka; Varma, Ajit.
Título: Bacterial community structure in soils contaminated with electronic waste pollutants from Delhi NCR, India
Fonte: Electron. j. biotechnol;41:72-80, sept. 2019. ilus, tab, graf.
Idioma: en.
Projeto: DST-SERB, Government of India, Young Scientist Scheme.
Resumo: Background: Microbial community analysis of electronic waste (e-waste)-polluted environments is of interest to understand the effect of toxic e-waste pollutants on the soil microbial community and to evaluate novel microorganisms resisting the toxic environment. The present study aims to investigate the bacterial community structure in soils contaminated with e-waste from various sites of Loni and Mandoli (National Capital Region (NCR), India) where e-waste dumping and recycling activities are being carried out for many years. Results: Interferences to soil metagenomic DNA extraction and PCR amplification were observed because of the presence of inhibiting components derived from circuit boards. Whole-metagenome sequencing on the Illumina MiSeq platform showed that the most abundant phyla were Proteobacteria and Firmicutes. Deltaproteobacteria and Betaproteobacteria were the most common classes under Proteobacteria. Denaturing gradient gel electrophoresis (DGGE) analysis of the bacterial 16S rRNA gene showed that e-waste contamination altered the soil bacterial composition and diversity. There was a decrease in the number of predominant bacterial groups like Proteobacteria and Firmicutes but emergence of Actinobacteria in the contaminated soil samples. Conclusions: This is the first report describing the bacterial community structure of composite soil samples of ewaste-contaminated sites of Loni and Mandoli, Delhi NCR, India. The findings indicate that novel bacteria with potential bioremediating properties may be present in the e-waste-contaminated sites and hence need to be evaluated further.
Descritores: Microbiologia do Solo
Bactérias/isolamento & purificação
Resíduo Eletrônico/análise
-Poluentes do Solo
Reação em Cadeia da Polimerase
Metais Pesados
Proteobactérias/isolamento & purificação
Eletroforese em Gel de Gradiente Desnaturante
Firmicutes/isolamento & purificação
Responsável: CL1.1 - Biblioteca Central

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Id: biblio-1087512
Autor: Han, Xufeng; Shao, Haiyan; Wang, Yi; Hu, Ankang; Chen, Renjin; Chen, Quangang.
Título: Composition of the bacterial community in the gastrointestinal tract of Kunming mice
Fonte: Electron. j. biotechnol;43:16-22, Jan. 2020. tab, graf.
Idioma: en.
Projeto: Scientific Research Foundation for the Excellent Talents of Xuzhou Medical University; . Natural Science Foundation of Jiangsu province; . Nature Science Foundation of Jiangsu Higher Education Institutions of China; . Applied Basic Research Program of Xuzhou Jiangsu.
Resumo: Background: The intestinal bacterial community has an important role in maintaining human health. Dysbiosis is a key inducer of many chronic diseases including obesity and diabetes. Kunming mice are frequently used as a model of human disease and yet little is known about the bacterial microbiome resident to the gastrointestinal tract. Results: We undertook metagenomic sequencing of the luminal contents of the stomach, duodenum, jejunum, ileum, cecum, colon, and rectum of Kunming mice. Firmicutes was the dominant bacterial phylum of each intestinal tract and Lactobacillus the dominant genus. However, the bacterial composition differed among the seven intestinal tracts of Kunming mice. Compared with the small intestine, the large intestine bacterial community of Kunming mice is more stable and diverse. Conclusions: To our knowledge, ours is the first study to systematically describe the gastrointestinal bacterial composition of Kunming mice. Our findings provide a better understanding of the bacterial composition of Kunming mice and serves as a foundation for the study of precision medicine.
Descritores: Bactérias/isolamento & purificação
Trato Gastrointestinal/microbiologia
RNA Ribossômico 16S
Reação em Cadeia da Polimerase
Sequenciamento de Nucleotídeos em Larga Escala
Firmicutes/isolamento & purificação
Microbioma Gastrointestinal
Lactobacillus/isolamento & purificação
Limites: Animais
Responsável: CL1.1 - Biblioteca Central

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