Base de dados : LILACS
Pesquisa : B03.353.500 [Categoria DeCS]
Referências encontradas : 3 [refinar]
Mostrando: 1 .. 3   no formato [Detalhado]

página 1 de 1

  1 / 3 LILACS  
              next record last record
para imprimir
Texto completo
Id: biblio-1253097
Autor: Wang, Longxing; Zhu, Wenjun; Gao, Zhen; Zhou, Hua; Cao, Fei; Jiang, Min; Li, Yan; Jia, Honghua; Wei, Ping.
Título: Biosynthetic L-tert-leucine using Escherichia coli co-expressing a novel NADH-dependent leucine dehydrogenase and a formate dehydrogenase
Fonte: Electron. j. biotechnol;47:83-88, sept. 2020. graf, ilus.
Idioma: en.
Projeto: National Key Research and Development Program of China.
Resumo: BACKGROUND: L-tert-Leucine has been widely used in pharmaceutical, chemical, and other industries as a vital chiral intermediate. Compared with chemical methods, enzymatic methods to produce L-tert-leucine have unparalleled advantages. Previously, we found a novel leucine dehydrogenase from the halophilic thermophile Laceyella sacchari (LsLeuDH) that showed good thermostability and great potential for the synthesis of L-tertleucine in the preliminary study. Hence, we manage to use the LsLeuDH coupling with a formate dehydrogenase from Candida boidinii (CbFDH) in the biosynthesis of L-tert-leucine through reductive amination in the present study. RESULT: The double-plasmid recombinant strain exhibited higher conversion than the single-plasmid recombinant strain when resting cells cultivated in shake flask for 22 h were used. Under the optimized conditions, the double-plasmid recombinant E. coli BL21 (pETDute-FDH-LDH, pACYCDute-FDH) transformed 1 mol·L-1 trimethylpyruvate (TMP) completely into L-tert-leucine with greater than 99.9% ee within 8 h. CONCLUSIONS: The LsLeuDH showed great ability to biosynthesize L-tert-leucine. In addition, it provided a new option for the biosynthesis of L-tert-leucine.
Descritores: Leucina Desidrogenase/metabolismo
Proteínas Recombinantes
Escherichia coli
Concentração de Íons de Hidrogênio
Responsável: CL1.1 - Biblioteca Central

  2 / 3 LILACS  
              first record previous record next record last record
para imprimir
Texto completo SciELO Brasil
Texto completo
Id: biblio-839377
Autor: Chauhan, Anjali; Guleria, Shiwani; Balgir, Praveen P; Walia, Abhishek; Mahajan, Rishi; Mehta, Preeti; Shirkot, Chand Karan.
Título: Tricalcium phosphate solubilization and nitrogen fixation by newly isolated Aneurinibacillus aneurinilyticus CKMV1 from rhizosphere of Valeriana jatamansi and its growth promotional effect
Fonte: Braz. j. microbiol;48(2):294-304, April.-June 2017. tab, graf.
Idioma: en.
Resumo: Abstract Aneurinibacillus aneurinilyticus strain CKMV1 was isolated from rhizosphere of Valeriana jatamansi and possessed multiple plant growth promoting traits like production of phosphate solubilization (260 mg/L), nitrogen fixation (202.91 nmol ethylene mL-1 h-1), indole-3-acetic acid (IAA) (8.1 µg/mL), siderophores (61.60%), HCN (hydrogen cyanide) production and antifungal activity. We investigated the ability of isolate CKMV1 to solubilize insoluble P via mechanism of organic acid production. High-performance liquid chromatography (HPLC) study showed that isolate CKMV1 produced mainly gluconic (1.34%) and oxalic acids. However, genetic evidences for nitrogen fixation and phosphate solubilization by organic acid production have been reported first time for A. aneurinilyticus strain CKMV1. A unique combination of glucose dehydrogenase (gdh) gene and pyrroloquinoline quinone synthase (pqq) gene, a cofactor of gdh involved in phosphate solubilization has been elucidated. Nitrogenase (nif H) gene for nitrogen fixation was reported from A. aneurinilyticus. It was notable that isolate CKMV1 exhibited highest antifungal against Sclerotium rolfsii (93.58%) followed by Fusarium oxysporum (64.3%), Dematophora necatrix (52.71%), Rhizoctonia solani (91.58%), Alternaria sp. (71.08%) and Phytophthora sp. (71.37%). Remarkable increase was observed in seed germination (27.07%), shoot length (42.33%), root length (52.6%), shoot dry weight (62.01%) and root dry weight (45.7%) along with NPK (0.74, 0.36, 1.82%) content of tomato under net house condition. Isolate CKMV1 possessed traits related to plant growth promotion, therefore, could be a potential candidate for the development of biofertiliser or biocontrol agent and this is the first study to include the Aneurinibacillus as PGPR.
Descritores: Reguladores de Crescimento de Plantas/metabolismo
Fosfatos de Cálcio/metabolismo
Lycopersicon esculentum/crescimento & desenvolvimento
Bacillales/isolamento & purificação
Fixação de Nitrogênio
-Microbiologia do Solo
Cromatografia Líquida de Alta Pressão
Lycopersicon esculentum/microbiologia
Raízes de Plantas/microbiologia
Fungos/crescimento & desenvolvimento
Responsável: BR1.1 - BIREME

  3 / 3 LILACS  
              first record previous record
para imprimir
Texto completo SciELO Brasil
Texto completo
Id: lil-741277
Autor: Venkadesaperumal, Gopu; Amaresan, Natrajan; Kumar, Krishna.
Título: Plant growth promoting capability and genetic diversity of bacteria isolated from mud volcano and lime cave of Andaman and Nicobar Islands
Fonte: Braz. j. microbiol;45(4):1271-1281, Oct.-Dec. 2014. ilus, tab.
Idioma: en.
Resumo: Twenty four bacterial strains from four different regions of mud volcano and lime cave were isolated to estimate their diversity, plant growth promoting and biocontrol activities to use them as inoculant strains in the fields. An excellent antagonistic effect against four plant pathogens and plant growth promoting properties such as IAA production, HCN production, phosphate solubilization, siderophore production, starch hydrolysis and hydrolytic enzymes syntheses were identified in OM5 (Pantoea agglomerans) and EM9 (Exiguobacterium sp.) of 24 studied isolates. Seeds (Chili and tomato) inoculation with plant growth promoting strains resulted in increased percentage of seedling emergence, root length and plant weight. Results indicated that co-inoculation gave a more pronounced effects on seedling emergence, secondary root numbers, primary root length and stem length, while inoculation by alone isolate showed a lower effect. Our results suggest that the mixed inocula of OM5 and EM9 strains as biofertilizers could significantly increase the production of food crops in Andaman archipelago by means of sustainable and organic agricultural system.
Descritores: Bacillales/isolamento & purificação
Microbiologia Ambiental
Lycopersicon esculentum/microbiologia
Desenvolvimento Vegetal
Pantoea/isolamento & purificação
Reguladores de Crescimento de Plantas/metabolismo
DNA Bacteriano/química
DNA Bacteriano/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Lycopersicon esculentum/fisiologia
Dados de Sequência Molecular
Raízes de Plantas/crescimento & desenvolvimento
Análise de Sequência de DNA
Plântula/crescimento & desenvolvimento
Responsável: BR1.1 - BIREME

página 1 de 1

Refinar a pesquisa
  Base de dados : Formulário avançado   

    Pesquisar no campo  

Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde