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Colen, Gecernir
Rosa, Carlos Augusto
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Id: biblio-974299
Autor: Penido, Fernanda Corrêa Leal; Piló, Fernanda Barbosa; Sandes, Sávio Henrique de Cicco; Nunes, Álvaro Cantini; Colen, Gecernir; Oliveira, Evelyn de Souza; Rosa, Carlos Augusto; Lacerda, Inayara Cristina Alves.
Título: Selection of starter cultures for the production of sour cassava starch in a pilot-scale fermentation process
Fonte: Braz. j. microbiol;49(4):823-831, Oct.-Dec. 2018. tab, graf.
Idioma: en.
Resumo: ABSTRACT Sour cassava starch (Polvilho azedo) is obtained from a spontaneous fermentation conducted by microorganisms from raw materials and fermentation tanks. This product is traditionally used in the baking industry for the manufacture of biscuits and Brazilian cheese breads. However, the end of fermentation is evaluated empirically, and the process occurs without standardization, which results in products of inconsistent quality. Predominant microbiota from a cassava flour manufacturer was isolated in order to select starter cultures for the production of sour cassava starch in a pilot-scale fermentation process. Lactic acid bacteria and yeasts were isolated, enumerated and grouped by Restriction Fragment Length Polymorphism, and PCR fingerprinting, respectively. One isolate of each molecular profile was identified by sequencing of the rRNA gene. LAB were prevalent throughout the entire process. Lactobacillus brevis (21.5%), which produced the highest values of acidity, and Lactobacillus plantarum (13.9%) were among the most frequent species. Pichia scutulata (52.2%) was the prevalent yeast and showed amylolytic activity. The aforementioned species were tested as single and mixed starter cultures in a pilot-scale fermentation process for 28 days. L. plantarum exhibited better performance as a starter culture, which suggests its potential for the production of sour cassava starch.
Descritores: Amido/metabolismo
Leveduras/metabolismo
Manihot/química
Lactobacillus/metabolismo
-Amido/química
Leveduras/genética
Brasil
Manihot/metabolismo
Fermentação
Microbiota
Microbiologia de Alimentos
Lactobacillus/isolamento & purificação
Lactobacillus/genética
Responsável: BR1.1 - BIREME


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Id: lil-788949
Autor: Peralta, Guillermo Hugo; Bergamini, Carina Viviana; Hynes, Erica Rut.
Título: Aminotransferase and glutamate dehydrogenase activities in lactobacilli and streptococci
Fonte: Braz. j. microbiol;47(3):741-748, July-Sept. 2016. tab, graf.
Idioma: en.
Resumo: ABSTRACT Aminotransferases and glutamate dehydrogenase are two main types of enzymes involved in the initial steps of amino acid catabolism, which plays a key role in the cheese flavor development. In the present work, glutamate dehydrogenase and aminotransferase activities were screened in twenty one strains of lactic acid bacteria of dairy interest, either cheese-isolated or commercial starters, including fifteen mesophilic lactobacilli, four thermophilic lactobacilli, and two streptococci. The strains of Streptococcus thermophilus showed the highest glutamate dehydrogenase activity, which was significantly elevated compared with the lactobacilli. Aspartate aminotransferase prevailed in most strains tested, while the levels and specificity of other aminotransferases were highly strain- and species-dependent. The knowledge of enzymatic profiles of these starter and cheese-isolated cultures is helpful in proposing appropriate combinations of strains for improved or increased cheese flavor.
Descritores: Streptococcus/enzimologia
Glutamato Desidrogenase/metabolismo
Transaminases/metabolismo
Lactobacillus/enzimologia
-Sistema Livre de Células
Ativação Enzimática
Microbiologia de Alimentos
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-1017969
Autor: Centuríon, María Gloria; Medina, Dorita; Alborno, Rose Marie; Laspina, Florntina; Amarilla, María Angela; Fariña, Norma; Ruiz, Antonia; Vergara, Juana; Paredes, María Elena.
Título: Estudio etiológico de infección de secrecion endocervical en un grupo de alto riesgo (prostistutas) / Study on the etiology of infections of endocervical secretions in a high risk group (prostitutes).
Fonte: Asunción; EFACIM; 1991. 227-232 p. tab.
Idioma: es.
Resumo: In order to determine the incidence and the etiology of the sexually trasnmitted diseaes (STD) 500 women, belonging to a high risk group (prostitutes), were studied from August, 1989 to Octuber 1990, and bacteriological and mycological studied were performed. Also, a questionnaire comprising detailed sexual behavior were rendered. High incidence of Gardnerella vaginalis (41.4 percent), Neisseria gonorrhoeae (12.2 percent), Trichomonas vaginalis (10.2 percent) and Candida albicans (10 percent) were found, in contrast with the low level found for Lactobacillus sp. The questinnaire revealed very low utilization of contraceptives as well as condom employement
Descritores: Candida albicans
Doenças Sexualmente Transmissíveis
Lactobacillus
Neisseria gonorrhoeae
Trichomonas vaginalis
Responsável: PY2.1 - Centro de Documentación
PY3.1 SR 616.9363 An78a 1991


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Id: biblio-839382
Autor: Rezvani, Fazlollah; Ardestani, Fatemeh; Najafpour, Ghasem.
Título: Growth kinetic models of five species of Lactobacilli and lactose consumption in batch submerged culture
Fonte: Braz. j. microbiol;48(2):251-258, April.-June 2017. tab, graf.
Idioma: en.
Resumo: Abstract Kinetic behaviors of five Lactobacillus strains were investigated with Contois and Exponential models. Awareness of kinetic behavior of microorganisms is essential for their industrial process design and scale up. The consistency of experimental data was evaluated using Excel software. L. bulgaricus was introduced as the most efficient strain with the highest biomass and lactic acid yield of 0.119 and 0.602 g g-1 consumed lactose, respectively. The biomass and carbohydrate yield of L. fermentum and L. lactis were slightly less and close to L. bulgaricus. Biomass and lactic acid production yield of 0.117 and 0.358 for L. fermentum and 0.114 and 0.437 g g-1 for L.actobacillus lactis were obtained. L. casei and L. delbrueckii had the less biomass yield, nearly 11.8 and 22.7% less than L. bulgaricus, respectively. L. bulgaricus (R 2 = 0.9500 and 0.9156) and L. casei (R 2 = 0.9552 and 0.8401) showed acceptable consistency with both models. The investigation revealed that the above mentioned models are not suitable to describe the kinetic behavior of L. fermentum (R 2 = 0.9367 and 0.6991), L. delbrueckii (R 2 = 0.9493 and 0.7724) and L. lactis (R 2 = 0.8730 and 0.6451). Contois rate equation is a suitable model to describe the kinetic of Lactobacilli. Specific cell growth rate for L. bulgaricus, L. casei, L. fermentum, L. delbrueckii and L. lactis with Contois model in order 3.2, 3.9, 67.6, 10.4 and 9.8-fold of Exponential model.
Descritores: Lactobacillus/crescimento & desenvolvimento
Lactobacillus/metabolismo
Lactose/metabolismo
Modelos Teóricos
-Edulcorantes/metabolismo
Biomassa
Ácido Láctico/metabolismo
Fermentação
Responsável: BR1.1 - BIREME


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Id: lil-601297
Autor: Castellano Filho, Didier Silveira; Diniz, Cláudio Galuppo; Silva, Vânia Lúcia da.
Título: Bacterial vaginosis: clinical, epidemiologic and microbiological features / Bacterial vaginosis: clinical, epidemiologic and microbiological features
Fonte: HU rev;36(3), jul.-set. 2010.
Idioma: pt.
Resumo: Bacterial Vaginosis (BV) is a polymicrobial clinical syndrome, whose etiology has not been fully understood. It occurs in approximately 30% of the women in childbearing age and is the result of the shift of protective resident microorganisms as Lactobacillus spp. by opportunistic pathogenic bacteria such as Gardnerella vaginalis. Patients with BV generally present copious, thin, homogeneous, milky, foul-smelling flow. Vaginal pH is > 4.5 and microscopy reveals bacteria-covered epithelial cells, termed “clue cells”. Around 50% of the patients are asymptomatic and the disease is associated with gynecologic complications, such as cervicitis, salpingitis, endometritis, post-operative infections and pelvic inflammatory disease; and obstetric complications, such as premature rupture of the membranes, preterm deliveries, chorioamniotitis and postpartum endometritis. Although the Nugent´s method is accepted as the “gold standard” for diagnosing BV, Amsel criteria are generally used for diagnosis in clinical practice. The Papanicolaou method is a valid diagnostic option, chiefly when it yields a positive result (mean specificity 95% when compared to gold standard). G. vaginalis has been almost universally recovered from women with BV, plays an important role in the pathogenesis of the disease, besides it may be detected in about 50% of healthy women. According to the literature, first-line drugs for BV treatment worldwide are metronidazole and clindamycin. However, regional studies on G. vaginalis drug susceptibility patterns are needed faced the so fast growing antimicrobial resistance phenomenon.
Descritores: Vaginose Bacteriana
-Gardnerella vaginalis
Vaginose Bacteriana/microbiologia
Lactobacillus
Metronidazol
Tipo de Publ: Relatos de Casos
Responsável: BR378.1 - Biblioteca Central


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Id: biblio-1021898
Autor: Strahsburger, Erwin; Lopez de Lacey, Ana Maria; Marotti, Ilaria; DiGioia, Diana; Biavati, Bruno; Dinelli, Giovanni.
Título: In vivo assay to identify bacteria with ß-glucosidase activity
Fonte: Electron. j. biotechnol;30:83-87, nov. 2017. graf, tab.
Idioma: en.
Projeto: University of Bologna; . Universidad Arturo Prat.
Resumo: Background: ß-Glucosidase assay is performed with purified or semipurified enzymes extracted from cell lysis. However, in screening studies, to find bacteria with ß-glucosidase activity among many tested bacteria, a fast method without cell lysis is desirable. In that objective, we report an in vivo ß-glucosidase assay as a fast method to find a ß-glucosidase producer strain. Results: The method consists in growing the strains for testing in a medium supplemented with the artificial substrate p-nitrophenyl-ß-glucopyranoside (pNPG). The presence of ß-glucosidases converts the substrate to p-nitrophenol (pNP), a molecule that can be easily measured in the supernatant spectrophotometrically at 405 nm. The assay was evaluated using two Bifidobacterium strains: Bifidobacterium longum B7254 strain that lacks ß-glucosidase activity and Bifidobacterium pseudocatenulatum B7003 strain that shows ß-glucosidase activity. The addition of sodium carbonate during pNP measurement increases the sensitivity of pNP detection and avoids the masking of absorbance by the culture medium. Furthermore, we show that pNP is a stable enzymatic product, not metabolized by bacteria, but with an inhibitory effect on cell growth. The ß-glucosidase activity was measured as units of enzyme per gram per minute per dry cell weight. This method also allowed the identification of Lactobacillus strains with higher ß-glucosidase activity among several lactobacillus species. Conclusion: This in vivo ß-glucosidase assay can be used as an enzymatic test on living cells without cell disruption. The method is simple, quantitative, and recommended, especially in studies screening for bacteria not only with ß-glucosidase activity but also with high ß-glucosidase activity.
Descritores: Bifidobacterium/isolamento & purificação
Bifidobacterium/enzimologia
beta-Glucosidase/metabolismo
-Bifidobacterium/metabolismo
Nitrofenilgalactosídeos
Ensaios Enzimáticos
Bifidobacterium longum/isolamento & purificação
Bifidobacterium longum/enzimologia
Bifidobacterium pseudocatenulatum/isolamento & purificação
Bifidobacterium pseudocatenulatum/enzimologia
Lactobacillus/isolamento & purificação
Lactobacillus/enzimologia
Lactobacillus/metabolismo
Nitrofenóis
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-840058
Autor: Talarico, Silvia T; Santos, Florenza E; Brandt, Katia Galeão; Martinez, Marina B; Taddei, Carla R.
Título: Anaerobic bacteria in the intestinal microbiota of Brazilian children
Fonte: Clinics;72(3):154-160, Mar. 2017. tab, graf.
Idioma: en.
Projeto: FAPESP.
Resumo: OBJECTIVE: Changes in the neonatal gut environment allow for the colonization of the mucin layer and lumen by anaerobic bacteria. The aim of the present study was to evaluate Bifidobacterium, Lactobacillus and Lactococcus colonization through the first year of life in a group of 12 Brazilian infants and to correlate these data with the levels of Escherichia coli. The presence of anaerobic members of the adult intestinal microbiota, including Eubacterium limosum and Faecalibacterium prausnitzii, was also evaluated. METHODS: Fecal samples were collected during the first year of life, and 16S rRNA from anaerobic and facultative bacteria was detected by real-time PCR. RESULTS: Bifidobacterium was present at the highest levels at all of the studied time points, followed by E. coli and Lactobacillus. E. limosum was rarely detected, and F. prausnitzii was detected only in the samples from the latest time points. CONCLUSION: These results are consistent with reports throughout the world on the community structure of the intestinal microbiota in infants fed a milk diet. Our findings also provide evidence for the influence of the environment on intestinal colonization due to the high abundance of E. coli. The presence of important anaerobic genera was observed in Brazilian infants living at a low socioeconomic level, a result that has already been well established for infants living in developed countries.
Descritores: Bactérias Anaeróbias/isolamento & purificação
Fezes/microbiologia
Microbioma Gastrointestinal
Intestinos/microbiologia
-Valores de Referência
Fatores de Tempo
Bactérias Anaeróbias/genética
Bifidobacterium/isolamento & purificação
Bifidobacterium/genética
Brasil
DNA Bacteriano
Fatores Etários
Carga Bacteriana
Reação em Cadeia da Polimerase em Tempo Real
Lactobacillus/isolamento & purificação
Lactobacillus/genética
Limites: Seres Humanos
Recém-Nascido
Lactente
Responsável: BR1.1 - BIREME


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Costa, Holmes Campanelli
Id: lil-180221
Autor: Salado, Gersislei Antonia; Costa, Holmes Campanelli; Oliveira, Antonio Joaquim de.
Título: Viabilidade de implantaçäo e sobrevivência de lactobacilos no trato intestinal de humanos adultos / Viability to implantation and survival of adult human intestinal tract.
Fonte: Bauru; Universidade do Sagrado Coraçäo; 1991. 59 p. tab, graf. (Cadernos de Divulgaçäo Cultural, 31).
Idioma: pt.
Resumo: Com o objetivo de estudar a viabilidade de implantaçao e a sobrevivência, além da açao dos lactobacilos no trato intestinal de humanos adultos, foram realizados dois tipos de tratamento, com duraçao de 45 dias ininterruptos. Os indivíduos foram divididos em dois grupos designados grupo A e grupo B. O grupo A consumiu iogurte natural e o grupo B consumiu iogurte natural e leite "in natura" tipo C. Para avaliar a açao dos lactobacilos sobre a flora intestinal e sua permanência no trato digestivo, foram efetuadas análises microbiológicas periódicas das fezes dos indivíduos. Os resultados obtidos, permitem concluir que os lactobacilos podem permanecer no intestino mesmo após a interrupçao da ingestao de iogurte, embora em números inferiores aos encontrados durante o período esperimental, e que somente com ingestao diária desse produto será possível sua persistência exercendo efeito controlador sobre a flora intestinal.
Descritores: Intestinos
Lactobacillus
-Iogurte
Ensaios Clínicos como Assunto
Gastroenteropatias/prevenção & controle
Limites: Seres Humanos
Masculino
Feminino
Adolescente
Adulto
Tipo de Publ: Estudo Comparativo
Responsável: BR1.1 - BIREME
BR1.1/2751.00; BR526.1; 612.33, S161v


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Id: lil-752482
Autor: Poppi, Larissa B; Rivaldi, Javier D; Coutinho, Thais S; Astolfi-Ferreira, Claudete S; Ferreira, Antonio J. Piantino; Mancilha, Ismael M.
Título: Effect of Lactobacillus sp. isolates supernatant on Escherichia coli O157: H7 enhances the role of organic acids production as a factor for pathogen control / Efeito do sobrenadante de isolados de Lactobacillus sp. sobre Escherichia coli O157: H7 reforça o papel da produção de ácidos orgânicos como fator de controle patogênico
Fonte: Pesqui. vet. bras = Braz. j. vet. res;35(4):353-359, 04/2015. tab, graf.
Idioma: en.
Resumo: Many attempts have been made to establish the control of foodborne pathogens through Lactobacillus isolates and their metabolism products with success being obtained in several situations. The aim of this study was to investigate the antagonistic effect of eight Lactobacillus isolates, including L. casei subsp. pseudoplantarum, L. plantarum, L. reuteri and L. delbrueckii subsp. delbrueckii, on the pathogenic Escherichia colistrain O157:H7. The inhibitory effect of pure cultures and two pooled cultures supernatants of Lactobacillus on the growth of pathogenic bacteria was evaluated by the spot agar method and by monitoring turbidity. Antimicrobial activity was confirmed for L. reuteri and L. delbrueckii subsp. delbrueckii and for a pool of lactic acid bacteria. The neutralized supernatant of the pool exerted a higher antimicrobial activity than that of the individual strains. Furthermore, D-lactic acid and acetic acid were produced during growth of the Lactobacillus isolates studied.(AU)

Muitas tentativas têm sido feitas para se estabelecer o controle de patógenos de origem alimentar através do uso de estirpes de Lactobacillus e dos seus produtos de metabolismo, com sucesso sendo sucedido em várias situações. O objetivo deste trabalho foi investigar o efeito antagônico do sobrenadante de culturas de oito isolados de Lactobacillus, incluindo L. casei subsp. pseudoplantarum, L. plantarum L. reuteri e L. delbrueckii subsp. delbrueckii, sobre Escherichia coli amostra O157:H7. Os efeitos inibidores de culturas puras e de dois "pools" de cultura de Lactobacillus sobre o crescimento da bactéria foram avaliados através do método de inibição em ágar e através do monitoramento da turbidez da cultura bacteriana. A atividade antimicrobiana foi confirmada para Lactobacillus reuteri e Lactobacillus delbrueckii subsp. delbrueckii e para o "pool" de bactérias acido-láctica. O sobrenadante neutralizado do "pool" de Lactobacillus exerceu uma atividade antimicrobiana mais elevada do que aquela das estirpes individuais. Além disso, ácido D-láctico e ácido acético foram produzidos durante o crescimento dos Lactobacillus estudados(AU)
Descritores: Escherichia coli O157
Ácido Acético/administração & dosagem
Ácido Láctico/administração & dosagem
Infecções por Escherichia coli/prevenção & controle
Lactobacillus
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: biblio-1009760
Autor: Zielinska, Krystyna; Fabiszewska, Agata; Swiatek, Michal; Szymanowska-Powalowska, Daria.
Título: Evaluation of the ability to metabolize 1, 2-propanediol by heterofermentative bacteria of the genus Lactobacillus
Fonte: Electron. j. biotechnol;26:60-63, Mar. 2017. ilus, ilus.
Idioma: en.
Resumo: Background: New directions of research on lactic acid bacteria include investigation of metabolic pathways for the synthesis and/or metabolism of 1,2-propanediol, commonly used in the food and chemical industry, medicine, pharmacy and cosmetology as well as agriculture. The objective of this study was to compare the capacity of strains representing three diverse heterofermentative species belonging to the genus Lactobacillus to synthesize and/or transform 1,2-PD as well as to suggest new directions of research aimed at commercial use of this metabolite. Results: The novel strain of Lactobacillus buchneri A KKP 2047p, characterized as exhibiting an unusual trait for that species in the form of capacity to metabolize 1,2-PD, grew poorly in a medium containing 1,2-PD as a sole carbon source. The supplementation with glucose facilitated rapid growth of bacteria and use of 1,2-PD for the synthesis of propionic acid. A similar observation was noted for Lactobacillus reuteri. On the other hand, Lactobacillus diolivorans effectively metabolized 1,2-PD which was the sole carbon source in the medium, and the addition of glucose inhibited the synthesis of propionic acid. The experiments also investigated the effect of cobalamin as a diol dehydratase coenzyme involved in the propionic acid synthesis from 1,2-PD whose addition promoted the yield of the reaction in the case of all tested strains. Conclusions: All tested isolates showed the ability to effectively metabolize 1,2-PD (in the presence of cobalamin) and its conversion to propionic acid, which reveals that investigated bacteria meet the essential requirements of microorganisms with a potential application.
Descritores: Propilenoglicol/metabolismo
Lactobacillus/metabolismo
-Propionatos
Vitamina B 12/metabolismo
Ácido Láctico
Propilenoglicol/síntese química
Fermentação
Glucose
Responsável: CL1.1 - Biblioteca Central



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