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Id: lil-780828
Autor: Mohamed, Yasmine Fathy; Abou-Shleib, Hamida Moustafa; Khalil, Amal Mohamed; El-Guink, Nadia Mohamed; El-Nakeeb, Moustafa Ahmed.
Título: Membrane permeabilization of colistin toward pan-drug resistant Gram-negative isolates
Fonte: Braz. j. microbiol;47(2):381-388, Apr.-June 2016. tab, graf.
Idioma: en.
Resumo: Abstract Pan-drug resistant Gram-negative bacteria, being resistant to most available antibiotics, represent a huge threat to the medical community. Colistin is considered the last therapeutic option for patients in hospital settings. Thus, we were concerned in this study to demonstrate the membrane permeabilizing activity of colistin focusing on investigating its efficiency toward those pan-drug resistant isolates which represent a critical situation. We determined the killing dynamics of colistin against pan-drug resistant isolates. The permeability alteration was confirmed by different techniques as: leakage, electron microscopy and construction of an artificial membrane model; liposomes. Moreover, selectivity of colistin against microbial cells was also elucidated. Colistin was proved to be rapid bactericidal against pan-drug resistant isolates. It interacts with the outer bacterial membrane leading to deformation of its outline, pore formation, leakage of internal contents, cell lysis and finally death. Furthermore, variations in membrane composition of eukaryotic and microbial cells provide a key for colistin selectivity toward bacterial cells. Colistin selectively alters membrane permeability of pan-drug resistant isolates which leads to cell lysis. Colistin was proved to be an efficient last line treatment for pan-drug resistant infections which are hard to treat.
Descritores: Membrana Celular/metabolismo
Infecções por Bactérias Gram-Negativas/microbiologia
Colistina/metabolismo
Farmacorresistência Bacteriana Múltipla
Bactérias Gram-Negativas/metabolismo
Antibacterianos/metabolismo
-Testes de Sensibilidade Microbiana
Membrana Celular/efeitos dos fármacos
Permeabilidade da Membrana Celular
Infecções por Bactérias Gram-Negativas/tratamento farmacológico
Colistina/farmacologia
Bactérias Gram-Negativas/isolamento & purificação
Bactérias Gram-Negativas/efeitos dos fármacos
Bactérias Gram-Negativas/ultraestrutura
Antibacterianos/farmacologia
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-983770
Autor: Berberian, Griselda; Brizuela, Martín; Rosanova, María T; Travaglianti, Mónica; Mastroiani, Alejandra; Reijtman, Vanesa; Fiorili, Graciela; Santa Cruz, Dora; Castro, Graciela.
Título: Infecciones por bacilos Gram-negativos multirresistentes en neonatología / Multidrug resistant Gram-negative infections in neonatology
Fonte: Arch. argent. pediatr;117(1):6-11, feb. 2019. tab.
Idioma: en; es.
Resumo: Introducción. Las infecciones por bacilos Gram-negativos multirresistentes (BGN-MR) constituyen un problema creciente en las unidades de cuidado intensivo neonatal. El objetivo del estudio fue conocer las características epidemiológicas, clínicas, microbiológicas, evolutivas y los factores de riesgo de infección por BGN-MR resistentes a carbapenemes en el Servicio de Neonatología de un hospital de alta complejidad. Población y método. Se realizó un estudio de cohorte retrospectivo en dicho Servicio, donde se incluyeron los pacientes con infección documentada por BGN-MR del 24/4/2013 al 29/4/2015. Resultados. Se incluyeron 21 pacientes. La mediana de edad gestacional y peso de nacimiento fue 35 semanas y 2070 gramos, respectivamente. Dieciocho pacientes (86 %) tuvieron hemocultivos positivos y el aislamiento microbiológico más frecuente fue Acinetobacter baumannii (17 pacientes, 81 %), seguido por Klebsiella pneumoniae productora de carbapenemasa (3 pacientes, 14 %) y Enterobacter cloacae (1 paciente, 5 %). La mediana de edad al momento del diagnóstico fue de 28 días y todos tenían factores de riesgo para la infección, como cirugía, asistencia respiratoria mecánica, nutrición parenteral, catéter central y antibióticos. El tratamiento antibiótico definitivo fue colistina en todos los casos, combinado en el 84 %. Cinco pacientes (24 %) fallecieron por la infección. La prematurez y el peso < 2000 g fueron factores de riesgo estadísticamente significativos asociados a la mortalidad (p = 0,03 y 0,01, respectivamente). Conclusión. Las infecciones por BGN-MR se presentaron en pacientes con factores predisponentes. Acinetobacter baumannii fue el primer agente etiológico. La mortalidad fue elevada y relacionada con prematurez y bajo peso al nacer.

Introduction. Multidrug resistant Gramnegative (MDRGN) infections are an increasing problem in neonatal intensive care units. The objective of this study was to establish the epidemiological, clinical, microbiological, and evolutionary characteristics of carbapenem-resistant MDRGN infections and the risk factors for them at the Division of Neonatology of a tertiary care hospital. Population and method. A retrospective cohort study was done in this Division in patients with a documented MDRGN infection between 4/24/2013 and 4/29/2015. Results. Twenty-one patients were included. Their median gestational age and birth weight were 35 weeks and 2070 g, respectively. Eighteen patients (86 %) had a positive blood culture; the most commonly isolated microorganism was Acinetobacter baumannii (17 patients, 81 %), followed by carbapenemase-producing Klebsiella pneumoniae (3 patients, 14 %) and Enterobacter cloacae (1 patient, 5 %). The median age at diagnosis was 28 days and all patients had risk factors for infection, including surgery, assisted mechanical ventilation, parenteral nutrition, central venous line, and antibiotics. The definite antibiotic therapy included colistin in all cases; in combination, in 84 %. Five patients (24 %) died due to the infection. Prematurity and a birth weight < 2000 g were statistically significant risk factors associated with mortality (p = 0.03 and 0.01, respectively). Conclusion. MDRGN infections were observed in patients with predisposing factors. Acinetobacter baumannii was the main etiologic agent. Mortality was high and related to prematurity and a low birth weight.
Descritores: Infecções por Bactérias Gram-Negativas/metabolismo
Infecções por Bactérias Gram-Negativas/tratamento farmacológico
Infecções por Bactérias Gram-Negativas/epidemiologia
-Epidemiologia Descritiva
Estudos Retrospectivos
Farmacorresistência Bacteriana Múltipla
Bactérias Gram-Negativas/metabolismo
Limites: Seres Humanos
Masculino
Feminino
Recém-Nascido
Tipo de Publ: Estudo Observacional
Responsável: AR94.1 - Centro de Información Pediatrica


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Id: biblio-997981
Autor: Weiler, Natalie; Ortiz, Flavia; Orrego, Maria; Huber, Claudia; Álvarez, Mercedes.
Título: Perfiles genéticos bacterianos y análisis de brotes de las Enfermedades Transmitidas por Alimentos empleando Electroforesis de Campo Pulsado como herramienta para la vigilancia epidemiológica molecular / Bacterial genetic profiles and analysis of outbreaks of foodborne diseases using pulsed field gel electrophoresis as a tool for molecular epidemiological surveillance
Fonte: Mem. Inst. Invest. Cienc. Salud (Impr.);16(2):65-78, Ago. 2018. tab, ilus.
Idioma: es.
Resumo: Las Enfermedades de Transmisión Alimentaria (ETA) son un problema de salud pública con altos índices de morbilidad y mortalidad a nivel global. La vigilancia y estudio de brotes de las ETA a través de Electroforesis de Campo Pulsado (PFGE) constituye un soporte fundamental para la investigación epidemiológica. El objetivo del estudio es presentar la base de datos de perfiles genéticos bacterianos y analizar brotes de enfermedades transmitidas por alimentos empleando Electroforesis de Campo Pulsado. Estudio descriptivo observacional de carácter retrospectivo, muestreo por conveniencia en el que fueron estudiados 778 aislamientos bacterianos causantes de ETA. La Base de Datos Nacional (BDN) quedó conformada por los siguientes patógenos entéricos; Salmonella spp., Shigella sonnei, Vibrio cholerae, Campylobacter spp., Escherichia coli O157:H7 y Escherichia coli no O157 caracterizados por una diversidad de patrones únicos, clusters y brotes. La BDN de Salmonella spp., quedó representada por un total de 558 cepas con 248 PUN, de las cuales 22,6% (126 cepas) corresponden a Salmonella enterica ser. Typhimurium, 20,6% (115 cepas) a Salmonella enterica ser. Enteritidis, 9,1% (51 cepas) a Salmonella enterica ser. Newport, 1,6% (9 cepas) a Salmonella enterica ser. Muenchen, que al mismo tiempo son los serotipos que están asociados a brotes. Fueron confirmados un total de 13 brotes causados por Salmonella spp.; Shigella sonnei con 113 cepas estudiadas, 57 patrones únicos y 19 clusters detectados. Se identificaron 3 patrones PYJ16X01.0012, PYJ16X01.0034 y PYJ16X01.0014 como los predominantes. Vibrio cholerae con 18 cepas estudiadas, 9 patrones únicos y 4 clusters detectados. Se pudo establecer una relación genética del 100% entre cepas de Vibrio cholerae O1 biotipo El Tor serotipo Ogawa productora de toxinas ctxA y tcpA aislada del caso índice del brote de cólera. Campylobacter spp., con 62 cepas estudiadas, 42 patrones únicos y 10 clusters detectados. La BDN de E. coli productor de toxina shiga O157 y no O157, con 9 y 20 cepas de origen humano respectivamente, caracterizadas según sus factores de virulencia y subtipos. Se reconocieron 8 patrones electroforéticos PUN y 1 cluster para E. coli productor de toxina shiga O157, y 18 PUN y 1 clúster para E. coli productor de toxina shiga no O157.La disponibilidad de una Base de Datos Nacional de patógenos bacterianos transmitidos por alimentos constituye un importante avance para la salud pública, con un gran aporte en la vigilancia y epidemiología del país permitiendo la confirmación y detección de brotes discriminando aislamientos relacionados genéticamente y por consiguiente el estudio de relaciones clonales y probable origen(AU)

Foodborne diseases (FBD) are a problem of public health with high indexes of morbidity and mortality at global level. The surveillance and study of outbreaks of the FBD through pulsed field gel electrophoresis (PFGE) is a fundamental support for epidemiological research. The aim of the study is to present the database of bacterial genetic profiles and analyze outbreaks of FBD using PFGE. This was an observational descriptive retrospective study with convenience sampling in which 778 bacterial isolates causing FBD were studied. The National Database (NDB) was made up of the following enteric pathogens causing FBD: Salmonella spp., Shigella sonnei, Vibrio cholerae, Campylobacter spp., Escherichia coli O157: H7 and Escherichia coli no O157. Each of them was characterized by a diversity of unique patterns, clusters and outbreaks. The NDB of Salmonella spp. was represented by a total of 558 strains with 248 PUN, of which 22.6% (126 strains) correspond to Salmonella enterica ser. Typhimurium, 20.6% (115 strains) to Salmonella enterica ser. Enteritidis, 9.1% (51 strains) to Salmonella enterica ser. Newport, 1.6% (9 strains) to Salmonella enterica ser. Muenchen, which at the same time are the serotypes associated with outbreaks. A total of thirteen outbreaks caused by Salmonella spp., Shigella sonnei with 113 strains studied, 57 unique patterns and 19 clusters detected were confirmed. Three patterns PYJ16X01.0012, PYJ16X01.0034 and PYJ16X01.0014 were identified as the predominant. Vibrio cholerae with 18 strains studied, 9 unique patterns and 4 clusters were detected. A genetic relationship of 100% was established between strains of Vibrio cholerae O1 biotype El Tor serotype Ogawa toxin producer ctxA and tcpA isolated from the index case of the cholera outbreak. Campylobacter spp., with 62 strains studied, 42 unique patterns and 10 clusters were detected. The NDB of O157 and non-O157 Shiga toxin producing E. coli O157, with 9 and 20 strains of human origin respectively, were characterized according to their virulence factors and subtypes. We recognized 8 PUN electrophoretic patterns and 1 cluster for O157 Shiga toxin producing E. coli, and 18 PUN and 1 cluster for non-O157 Shiga toxin producing E. coli. The availability of a National Database of bacterial pathogens transmitted by food constitutes an important advance for public health, with a great contribution to the surveillance and epidemiology of the country allowing the confirmation and detection of outbreaks discriminating genetically related isolates and therefore, the study of clonal relationships and probable origin(AU)
Descritores: Eletroforese em Gel de Campo Pulsado
Doenças Transmitidas por Alimentos/microbiologia
Bactérias Gram-Negativas/genética
-Paraguai/epidemiologia
Surtos de Doenças
Estudos Retrospectivos
Doenças Transmitidas por Alimentos/epidemiologia
Tipo de Publ: Estudo Observacional
Responsável: PY3.1 - Biblioteca


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Id: biblio-839385
Autor: Tian, Mengyang; Du, Dongyun; Zhou, Wei; Zeng, Xiaobo; Cheng, Guojun.
Título: Phenol degradation and genotypic analysis of dioxygenase genes in bacteria isolated from sediments
Fonte: Braz. j. microbiol;48(2):305-313, April.-June 2017. tab, graf.
Idioma: en.
Projeto: Natural Science Foundation of Hubei Province; . Fundamental Research Funds for the Central Universities, South-Central University for Nationalities.
Resumo: Abstract The aerobic degradation of aromatic compounds by bacteria is performed by dioxygenases. To show some characteristic patterns of the dioxygenase genotype and its degradation specificities, twenty-nine gram-negative bacterial cultures were obtained from sediment contaminated with phenolic compounds in Wuhan, China. The isolates were phylogenetically diverse and belonged to 10 genera. All 29 gram-negative bacteria were able to utilize phenol, m-dihydroxybenzene and 2-hydroxybenzoic acid as the sole carbon sources, and members of the three primary genera Pseudomonas, Acinetobacter and Alcaligenes were able to grow in the presence of multiple monoaromatic compounds. PCR and DNA sequence analysis were used to detect dioxygenase genes coding for catechol 1,2-dioxygenase, catechol 2,3-dioxygenase and protocatechuate 3,4-dioxygenase. The results showed that there are 4 genotypes; most strains are either PNP (catechol 1,2-dioxygenase gene is positive, catechol 2,3-dioxygenase gene is negative, protocatechuate 3,4-dioxygenase gene is positive) or PNN (catechol 1,2-dioxygenase gene is positive, catechol 2,3-dioxygenase gene is negative, protocatechuate 3,4-dioxygenase gene is negative). The strains with two dioxygenase genes can usually grow on many more aromatic compounds than strains with one dioxygenase gene. Degradation experiments using a mixed culture representing four bacterial genotypes resulted in the rapid degradation of phenol. Determinations of substrate utilization and phenol degradation revealed their affiliations through dioxygenase genotype data.
Descritores: Fenol/metabolismo
Dioxigenases/genética
Dioxigenases/metabolismo
Bactérias Gram-Negativas/enzimologia
Bactérias Gram-Negativas/metabolismo
-Filogenia
Pseudomonas
Poluentes do Solo/metabolismo
Acinetobacter
DNA Bacteriano/genética
DNA Bacteriano/química
DNA Ribossômico/genética
DNA Ribossômico/química
Carbono/metabolismo
RNA Ribossômico 16S/genética
Biotransformação
Análise por Conglomerados
China
Reação em Cadeia da Polimerase
Análise de Sequência de DNA
Sedimentos Geológicos/microbiologia
Alcaligenes
Poluição Ambiental
Bactérias Gram-Negativas/classificação
Bactérias Gram-Negativas/genética
Responsável: BR1.1 - BIREME


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Id: biblio-839378
Autor: Campana, Eloiza H; Chuster, Stephanie G; Silva, Isadora R. da; Paschoal, Raphael P; Bonelli, Raquel R; Moreira, Beatriz M; Picão, Renata C.
Título: Modified Carba NP test for the detection of carbapenemase production in gram-negative rods: optimized handling of multiple samples
Fonte: Braz. j. microbiol;48(2):242-245, April.-June 2017. tab, graf.
Idioma: en.
Resumo: Abstract The modified Carba NP test presented here may be a valuable tool for laboratories interested in investigating a large number of carbapenemase-producing bacteria in a less-costly way. The test was evaluated against 48 carbapenemase-producing and carbapenemase-non-producing gram-negative bacteria. No false–positive results were obtained, but false-negative results were observed with OXA-23- and GES-carbapenemase-producing isolates. Aeromonas sp. are not testable by Modified Carba NP.
Descritores: Proteínas de Bactérias/análise
beta-Lactamases/análise
Técnicas Bacteriológicas/métodos
Bactérias Gram-Negativas/enzimologia
-Reações Falso-Negativas
Responsável: BR1.1 - BIREME


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Id: biblio-894863
Autor: Peiris, Mudara K; Gunasekara, Chinthika P; Jayaweera, Pradeep M; Arachchi, Nuwan DH; Fernando, Neluka.
Título: Biosynthesized silver nanoparticles: are they effective antimicrobials?
Fonte: Mem. Inst. Oswaldo Cruz;112(8):537-543, Aug. 2017. tab, graf.
Idioma: en.
Projeto: University of Sri Jayewardenepura.
Resumo: BACKGROUND Silver nanoparticles (AgNPs) are increasingly being used in medical applications. Therefore, cost effective and green methods for generating AgNPs are required. OBJECTIVES This study aimed towards the biosynthesis, characterisation, and determination of antimicrobial activity of AgNPs produced using Pseudomonas aeruginosa ATCC 27853. METHODS Culture conditions (AgNO3 concentration, pH, and incubation temperature and time) were optimized to achieve maximum AgNP production. The characterisation of AgNPs and their stability were evaluated by UV-visible spectrophotometry and scanning electron microscopy. FINDINGS The characteristic UV-visible absorbance peak was observed in the 420-430 nm range. Most of the particles were spherical in shape within a size range of 33-300 nm. The biosynthesized AgNPs exhibited higher stability than that exhibited by chemically synthesized AgNPs in the presence of electrolytes. The biosynthesized AgNPs exhibited antimicrobial activity against Escherichia coli, P. aeruginosa, Salmonella typhimurium, Staphylococcus aureus, methicillin-resistant S. aureus, Acinetobacter baumannii, and Candida albicans. MAIN CONCLUSION As compared to the tested Gram-negative bacteria, Gram-positive bacteria required higher contact time to achieve 100% reduction of colony forming units when treated with biosynthesized AgNPs produced using P. aeruginosa.
Descritores: Prata/farmacologia
Contagem de Colônia Microbiana/métodos
Nanopartículas Metálicas/química
Bactérias Gram-Negativas/metabolismo
Bactérias Gram-Negativas/ultraestrutura
Bactérias Gram-Positivas/efeitos dos fármacos
Antibacterianos/biossíntese
Antibacterianos/farmacologia
Antibacterianos/química
-Pseudomonas aeruginosa
Espectrofotometria
Microscopia Eletrônica/métodos
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-889236
Autor: Capizzani, Carolina Paulino da Costa; Caçador, Natália Candido; Marques, Elizabeth Andrade; Levy, Carlos Emílio; Tonani, Ludmilla; Torres, Lidia Alice Gomes Monteiro Marin; Darini, Ana Lúcia da Costa.
Título: A practical molecular identification of nonfermenting Gram-negative bacteria from cystic fibrosis
Fonte: Braz. j. microbiol;49(2):422-428, Apr.-June 2018. tab, graf.
Idioma: en.
Projeto: São Paulo Research Foundation; . National Council for Scientific and Technological Development.
Resumo: Abstract Identification of nonfermenting Gram-negative bacteria (NFGNB) of cystic fibrosis patients is hard and misidentification could affect clinical outcome. This study aimed to propose a scheme using polymerase chain reaction to identify NFGNB. This scheme leads to reliable identification within 3 days in an economically viable manner when compared to other methods.
Descritores: Reação em Cadeia da Polimerase/métodos
Infecções por Bactérias Gram-Negativas/diagnóstico
Fibrose Cística/complicações
Técnicas de Diagnóstico Molecular/métodos
Bactérias Gram-Negativas/isolamento & purificação
-Fatores de Tempo
Bactérias Gram-Negativas/genética
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-886161
Autor: Kin, Marta Susana; Giménez, Hugo; Fort, Marcelo Cristian.
Título: La técnica de polarización fluorescente para el diagnóstico de Brucella en Chaetophractus villosus / Technique of fluorescence polarization for the diagnosis of Brucella in Chaetophractus villosus / A técnica de polarização fluorescente para o diagnóstico de Brucella em Chaetophractus villosus
Fonte: Acta bioquím. clín. latinoam;52(1):61-64, mar. 2018. tab.
Idioma: es.
Projeto: UNLPam; . INTA.
Resumo: La brucelosis es una enfermedad muy extendida por todo el mundo. El diagnóstico se realiza mediante el uso de diferentes técnicas, entre ellas el aislamiento del agente etiológico o por serología detectando anticuerpos específicos. Todos estos métodos están estandarizados y validados en general para las distintas especies animales de interés zootécnico. En animales silvestres pueden utilizarse los mismos procedimientos serológicos, pero cada uno debe ser validado para la especie animal estudiada. El objetivo de este trabajo fue comprobar si la técnica de Polarización Fluorescente (FPA) utilizada para la determinación de anticuerpos contra Brucella en bovinos puede ser usada para el diagnóstico de Brucella en el armadillo (Chaetophractus villosus). Para ello se tomaron 150 muestras de sangre de Chaetophractus villosus. Veinticuatro muestras fueron positivas por medio de las técnicas de antígeno bufferado en placa, seroaglutinación lenta en tubo, 2-mercaptoetanol y fijación del complemento. Las mismas muestras resultaron positivas a la técnica de Polarización Fluorescente, estableciéndose un límite de corte de 82 mP. El índice Kappa registrado fue de 1 para todos los tests de diagnóstico comparados con FPA (IC: 0,84-1).

Brucellosis is a disease widespread throughout the world. The diagnosis is made through the use of different techniques including the isolation of the etiological agent or by serology detecting specific antibodies. All these methods are standardized and validated in general for animal species of zoo-technical interest. In wild animals, serological procedures may be used, but each should be validated for the particular animal species studied. The objective of this work was to verify if the technique of Fluorescent Polarization used for the determination of antibodies against Brucella in ruminants can be used for the diagnosis of Brucella in Chaetophractus villosus. To this aim, s 150 blood samples of Chaetophractus villosus were taken. Twenty-four samples were positive by the techniques of Antigen Buffered in Plate, serum agglutination test (SAT), 2-mercaptoethanol (2-ME) agglutination test and complement fixation test (CFT). The same samples were also positive by Fluorescent Polarization, and a cut-off limit of 82 mP was established. The KAPPA index was 1 for all diagnostic tests compared to FPA (CI: 0.84-1).

A brucelose é uma doença muito espalhada pelo mundo. O diagnóstico é feito por meio de diferentes técnicas, incluindo o isolamento do agente etiológico ou por sorologia detectando anticorpos específicos. Todos esses métodos são padronizados e validados em geral para diferentes espécies animais de interesse zootécnico. Em animais silvestres podem ser utilizados os mesmos métodos serológicos, mas cada um tem de ser validado para a espécie de animal estudada. O objectivo deste estudo foi testar se a técnica de Polarização Fluorescente utilizada para a determinação de anticorpos contra Brucella em bovinos pode ser utilizada para o diagnóstico de Brucella em tatu (Chaetophractus villosus). Para isso, 150 amostras de sangue de Chaetophractus villosus foram coletadas. Vinte e quatro amostras foram positivas por técnicas de antígeno bufferado em placa, soroaglutinação lenta em tubo, 2-mercaptoetanol e fixação do complemento. As mesmas amostras foram positivas para a técnica de Polarização Fluorescente, estabelecendo um limite de corte de 82 mP. O índice Kappa registrado foi de 1 para todos os testes de diagnóstico em comparação com FPA (CI: 0,84-1).
Descritores: Brucella
Diagnóstico
Polarização de Fluorescência/métodos
-Sorologia
Sorologia/instrumentação
Bactérias Gram-Negativas
Responsável: AR1.1 - Biblioteca Rafael Herrera Vegas


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Id: biblio-841158
Autor: MIRANDA, Tamires Szeremeske; FERES, Magda; RETAMAL-VALDÉS, Belén; PEREZ-CHAPARRO, Paula Juliana; MACIEL, Suellen Silva; DUARTE, Poliana Mendes.
Título: Influence of glycemic control on the levels of subgingival periodontal pathogens in patients with generalized chronic periodontitis and type 2 diabetes
Fonte: J. appl. oral sci;25(1):82-89, Jan.-Feb. 2017. tab, graf.
Idioma: en.
Projeto: São Paulo Research Foundation; . São Paulo Research Foundation.
Resumo: Abstract Objective This study evaluated the influence of glycemic control on the levels and frequency of subgingival periodontal pathogens in patients with type 2 diabetes mellitus (DM) and generalized chronic periodontitis (ChP). Material and Methods Fifty-six patients with generalized ChP and type 2 DM were assigned according to the levels of glycated hemoglobin (HbA1c) into one of the following groups: HbA1c<8% (n=28) or HbA1c≥8% (n=28). Three subgingival biofilm samples from sites with probing depth (PD)<5 mm and three samples from sites with PD≥5 mm were analyzed by quantitative Polymerase Chain Reaction (PCR) for the presence and levels of Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Eubacterium nodatum, Parvimona micra, Fusobacterium nucleatum ssp. and Prevotella intermedia. Results The mean counts of F. nucleatum ssp. were statistically significantly higher in the sites with PD≥5 mm of the HbA1c≥8% group (p<0.05). Frequencies of detection of T. forsythia, E. nodatum, P. micra and F. nucleatum ssp. were all higher in the sites with PD≥5 mm of the patients with HbA1c≥8%, compared with those of patients with HbA1c<8% (p<0.05). Frequency of detection of P. intermedia was higher in the sites with PD<5 mm of the patients with HbA1c≥8% than those of the patients with HbA1c<8% (p<0.05). Conclusions Poor glycemic control, as indicated by HbA1c≥8%, is associated with increased levels and frequencies of periodontal pathogens in the subgingival biofilm of subjects with type 2 DM and ChP.
Descritores: Glicemia/análise
Diabetes Mellitus Tipo 2/microbiologia
Diabetes Mellitus Tipo 2/terapia
Periodontite Crônica/microbiologia
Periodontite Crônica/sangue
Gengiva/microbiologia
-Contagem de Colônia Microbiana
Resultado do Tratamento
Estatísticas não Paramétricas
Biofilmes
Placa Dentária/microbiologia
Diabetes Mellitus Tipo 2/complicações
Carga Bacteriana
Reação em Cadeia da Polimerase em Tempo Real
Bactérias Gram-Negativas/isolamento & purificação
Hiperglicemia/prevenção & controle
Limites: Seres Humanos
Masculino
Feminino
Adulto
Meia-Idade
Tipo de Publ: Estudos de Avaliação
Responsável: BR1.1 - BIREME


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Texto completo SciELO Brasil
Timm, Claudio Dias
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Id: biblio-1026529
Autor: Tavares, Alana Borges; Cereser, Natacha Deboni; Timm, Cláudio Dias.
Título: Ocorrência de Aeromonas spp. em alimentos de origem animal e sua importância em saúde pública / Occurence of Aeromonas spp. in foods of animal origin and its importance in public health
Fonte: Arq. Inst. Biol;82:1-8, 2015.
Idioma: pt.
Resumo: Aeromonas spp. são bactérias Gram negativas, oportunistas, de natureza ubíqua, isoladas principalmente de amostras de água. Até o presente momento foram reconhecidas 31 espécies, sendo as de maior importância médica Aeromonas hydrophila, Aeromonas caviae e Aeromonas veronii. A patogenicidade do gênero é considerada multifatorial, sendo este produtor de diversos tipos de toxinas e com envolvimento de outros fatores capazes de facilitar a penetração e o estabelecimento do agente no hospedeiro, causando doença. O objetivo desta revisão é elucidar o papel dos alimentos de origem animal como fontes de contaminação de bactérias do gênero Aeromonas para o ser humano. Isolamentos de aeromonas de diversos produtos de origem animal têm sido relatados, como carne, leite e seus derivados, além de frutos do mar, e em ambientes de processamento, como abatedouros, frigorífcos e laticínios. Tem-se buscado determinar fontes de contaminação dos alimentos, e a água foi definida como o principal disseminador. Aeromonas já foi definida como sendo a causadora de diversas enfermidades, desde afecções gastrointestinais até casos de meningite e morte. Considerando os alimentos de origem animal como importantes veículos de transmissão para o ser humano e o reconhecimento da água como fonte de disseminação do agente, torna-se imprescindível o tratamento adequado da água utilizada nos estabelecimentos processadores de alimentos para a segurança alimentar.(AU)

Aeromonas spp. are opportunistic, ubiquitous Gram negative bacteria, mostly isolated from water samples. Until the present time, 31 species have been recognized, and the most medically important are Aeromonas hydrophila, Aeromonas caviae and Aeromonas veronii. The pathogenicity of the genus is considered to be multifactorial, and it can produce several types of toxins, with the involvement of other factors that facilitate the penetration and the establishment of the agent in the host, thus causing the disease. Te objective of this review is to elucidate the role of foods of animal origin as sources of contamination of aeromonas to humans. Aeromonas have been reported in various animal products such as meat, milk, dairy products, and seafood, and also in processing environments such as slaughterhouses, meat and dairy plants. There has been the attempt to determine sources of food contamination, being the water defined as the main disseminator. Aeromonas has been defined as the cause of many diseases, from gastrointestinal affections to cases of meningitis and even death. Considering that animal foods are an important source of contamination for humans and because of the recognition of water as a source of dissemination, it is essential for food security to provide the proper treatment of water used in food processing establishments.(AU)
Descritores: Virulência
Contaminação de Alimentos
Aeromonas/patogenicidade
Alimentos de Origem Animal
Bactérias Gram-Negativas
-Diarreia
Microbiologia de Alimentos
Noxas
Responsável: BR1942.1 - NID - Biblioteca - Núcleo de Informação e Documentação



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