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Id: lil-755821
Autor: Gu, Quan; Li, Xunde; Qu, Pinghua; Hou, Shuiping; Li, Juntao; Atwill, Edward R.; Chen, Shouyi.
Título: Characterization of Francisella species isolated from the cooling water of an air conditioning system
Fonte: Braz. j. microbiol;46(3):921-927, July-Sept. 2015. tab, ilus.
Idioma: en.
Projeto: Guangzhou Science and Technology Program; . Key Medicine Discipline Construction of Guangzhou Municipality.
Resumo:

Strains of Francisella spp. were isolated from cooling water from an air conditioning system in Guangzhou, China. These strains are Gram negative, coccobacilli, non-motile, oxidase negative, catalase negative, esterase and lipid esterase positive. In addition, these bacteria grow on cysteine-supplemented media at 20 °C to 40 °C with an optimal growth temperature of 30 °C. Analysis of 16S rRNA gene sequences revealed that these strains belong to the genus Francisella. Biochemical tests and phylogenetic and BLAST analyses of 16S rRNA, rpoB and sdhA genes indicated that one strain was very similar to Francisella philomiragia and that the other strains were identical or highly similar to the Francisella guangzhouensis sp. nov. strain 08HL01032 we previously described. Biochemical and molecular characteristics of these strains demonstrated that multiple Francisella species exist in air conditioning systems.

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Descritores: Ar Condicionado
Proteínas de Bactérias/genética
RNA Polimerases Dirigidas por DNA/genética
Francisella
Flavoproteínas/genética
Microbiologia da Água
-Sequência de Bases
China
DNA Bacteriano/genética
DNA Ribossômico/genética
Francisella/classificação
Francisella/genética
Francisella/isolamento & purificação
Dados de Sequência Molecular
Tipagem Molecular
Filogenia
/genética
RNA, RIBOSOMAL, ABNORMALITIES, MULTIPLES/genética
Análise de Sequência de DNA
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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