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Pesquisa : B03.440.400.425.625.625.675 [Categoria DeCS]
Referências encontradas : 17 [refinar]
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Id: biblio-1047375
Autor: Li, Shanshan; Qin, Kun; Li, Huaying; Guo, Jin; Li, Dejin; Liu, Fang; Tan, Zhilei; Yan, Wei; Qu, Shuling; Zhao, Huabing.
Título: Cloning and characterisation of four catA genes located on the chromosome and large plasmid of Pseudomonas putida ND6
Fonte: Electron. j. biotechnol;34:83-90, july. 2018. tab, ilus, graf.
Idioma: en.
Projeto: Tianjin Research Program of Application Foundation and Advanced Technology; . National Natural Science Foundation of China.
Resumo: Background: Although the functional redundancy of catechol 1,2-dioxygenase (C12O) genes has been reported in several microorganisms, limited enzymes were characterised, let alone the advantage of the coexistence of the multiple copies of C12O genes. Results: In this study, four novel C12O genes, designated catA, catAI, catAII and catAIII, in the naphthalene-degrading strain Pseudomonas putida ND6, were cloned and characterised. Phylogenetic analysis of their deduced amino acid sequences revealed that the four C12O isozymes each formed independent subtrees, together with homologues from other organisms. All four enzymes exhibited maximum activity at pH 7.4 and higher activity in alkaline than in acidic conditions. Furthermore, CatA, CatAI and CatAIII were maximally active at a temperature of 45°C, whereas a higher optimum temperature was observed for CatAII at a temperature of 50°C. CatAI exhibited superior temperature stability compared with the other three C12O isozymes, and kinetic analysis indicated similar enzyme activities for CatA, CatAI and CatAII, whereas that of CatAIII was lower. Significantly, among metal ions tested, only Cu2+ substantially inhibited the activity of these C12O isozymes, thus indicating that they have potential to facilitate bioremediation in environments polluted with aromatics in the presence of metals. Moreover, gene expression analysis at the mRNA level and determination of enzyme activity clearly indicated that the redundancy of the catA genes has increased the levels of C12O. Conclusion: The results clearly imply that the redundancy of catA genes increases the available amount of C12O in P. putida ND6, which would be beneficial for survival in challenging environments.
Descritores: Pseudomonas putida/enzimologia
Pseudomonas putida/genética
Catecol 1,2-Dioxigenase/genética
-Temperatura
Biodegradação Ambiental
Clonagem Molecular
Catecol 1,2-Dioxigenase/análise
Catecol 1,2-Dioxigenase/metabolismo
Genes Bacterianos
Concentração de Íons de Hidrogênio
Isoenzimas
Metais
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-984009
Autor: Munir, I; Bano, A; Faisal, M.
Título: Impact of phosphate solubilizing bacteria on wheat (Triticum aestivum ) in the presence of pesticides / Impacto das bactérias solubilizantes de fosfato no trigo (Triticum aestivum ) na presença de pesticidas
Fonte: Braz. j. biol;79(1):29-37, Jan.-Mar 2019. tab, graf.
Idioma: en.
Resumo: Abstract Three phosphate solubilizing bacteria were isolated and identified by 16S rRNA sequencing as Pseudomonas putida, Pseudomonas sp and Pseudomonas fulva . The strains were subjected to plant biochemical testing and all the PGPR attributes were checked in the presence of pesticides (chlorpyrifos and pyriproxyfen). The phosphate solubilizing index of strain Ros2 was highest in NBRIP medium i.e 2.23 mm. All the strains showed acidic pH (ranges from 2.5-5) on both medium i.e PVK and NBRIP. Strain Ros2 was highly positive for ammonia production as well as siderophore production while strain Rad2 was positive for HCN production. The results obtained by the strains Rad1, Rad2 and Ros2 for auxin production were 33.1, 30.67 and 15.38 µg ml-1, respectively. Strain Rad1 showed 16% increase in percentage germination in comparison to control in the presence of pesticide stress. Most promising results for chlorophyll content estimation were obtained in the presence of carotenoids upto 6 mgg-1 without stress by both strains Rad1 and Rad2. Study suggests that especially strain Ros2 can enhance plant growth parameters in the pesticide stress.

Resumo Três bactérias solubilizantes de fosfato foram isoladas e identificadas por seqüenciamento de rRNA 16S como Pseudomonas putida, Pseudomonas sp e Pseudomonas fulva. As estirpes foram submetidas a testes bioquímicos de plantas e todos os atributos PGPR foram verificados na presença de pesticidas (clorpirifos e piriproxifeno). O índice de solubilização de fosfato da estirpe Ros2 foi mais elevado no meio NBRIP, isto é, 2,23 mm. Todas as estirpes apresentaram um pH ácido (varia de 2,5-5) em ambos os meios, isto é PVK e NBRIP. A estirpe Ros2 foi altamente positiva para a produção de amoníaco, bem como a produção de sideróforos enquanto a estirpe Rad2 foi positiva para a produção de HCN. Os resultados obtidos pelas estirpes Rad1, Rad2 e Ros2 para a produção de auxina foram 33,1, 30,67 e 15,38 μg ml-1 , respectivamente. A deformação Rad1 mostrou aumento de 16% na germinação percentual em comparação com o controlo na presença de stress de pesticida. Os resultados mais promissores para a estimativa do teor de clorofila foram obtidos na presença de carotenóides até 6 mgg-1 sem estresse por ambas as cepas Rad1 e Rad2. Estudo sugere que especialmente a estirpe Ros2 pode melhorar parâmetros de crescimento de plantas no estresse de pesticidas.
Descritores: Fosfatos/metabolismo
Pseudomonas/fisiologia
Piridinas/administração & dosagem
Triticum/crescimento & desenvolvimento
Clorpirifos/administração & dosagem
Inseticidas/administração & dosagem
-Paquistão
Pseudomonas/efeitos dos fármacos
Triticum/metabolismo
Triticum/microbiologia
RNA Bacteriano/análise
RNA Ribossômico 16S/análise
Pseudomonas putida/efeitos dos fármacos
Pseudomonas putida/fisiologia
Análise de Sequência de RNA
Responsável: BR1.1 - BIREME


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Id: biblio-889215
Autor: Singh, Utkarsh; Arora, Naveen Kumar; Sachan, Preeti.
Título: Simultaneous biodegradation of phenol and cyanide present in coke-oven effluent using immobilized Pseudomonas putida and Pseudomonas stutzeri
Fonte: Braz. j. microbiol;49(1):38-44, Jan.-Mar. 2018. tab, graf.
Idioma: en.
Resumo: ABSTRACT Discharge of coke-oven wastewater to the environment may cause severe contamination to it and also threaten the flora and fauna, including human beings. Hence before dumping it is necessary to treat this dangerous effluent in order to minimize the damage to the environment. Conventional technologies have inherent drawbacks however, biological treatment is an advantageous alternative method. In the present study, bacteria were isolated from the soil collected from the sites contaminated by coke-oven effluent rich in phenol and cyanide. Nucleotides sequence alignment and phylogenetic analysis showed the identity of the selected phenol and cyanide degrading isolates NAUN-16 and NAUN-1B as Pseudomonas putida and Pseudomonas stutzeri, respectively. These two isolates tolerated phenol up to 1800 mg L-1 and cyanide up to 340 mg L-1 concentrations. The isolates were immobilized on activated charcoal, saw dust and fly ash. The effluent was passed through the column packed with immobilized cells with a flow rate of 5 mL min-1. The isolates showed degradation of phenol up to 80.5% and cyanide up to 80.6% and also had the ability to reduce biological oxygen demand, chemical oxygen demand and lower the pH of effluent from alkaline to near neutral. The study suggests the utilization of such potential bacterial strains in treating industrial effluent containing phenol and cyanide, before being thrown in any ecosystem.
Descritores: Cianetos/metabolismo
Fenol/metabolismo
Pseudomonas putida/metabolismo
Pseudomonas stutzeri/metabolismo
Eliminação de Resíduos Líquidos/métodos
Águas Residuárias/microbiologia
-Biodegradação Ambiental
Células Imobilizadas/classificação
Células Imobilizadas/metabolismo
Coque/análise
Cianetos/análise
Resíduos Industriais/análise
Fenol/análise
Filogenia
Pseudomonas putida/classificação
Pseudomonas putida/genética
Pseudomonas putida/isolamento & purificação
Pseudomonas stutzeri/classificação
Pseudomonas stutzeri/genética
Pseudomonas stutzeri/isolamento & purificação
Águas Residuárias/análise
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: lil-755822
Autor: Kandasamy, Sujatha; Dananjeyan, Balachandar; Krishnamurthy, Kumar; Benckiser, Gero.
Título: Aerobic cyanide degradation by bacterial isolates from cassava factory wastewater
Fonte: Braz. j. microbiol;46(3):659-666, July-Sept. 2015. ilus.
Idioma: en.
Resumo:

Ten bacterial strains that utilize cyanide (CN) as a nitrogen source were isolated from cassava factory wastewater after enrichment in a liquid media containing sodium cyanide (1 mM) and glucose (0.2% w/v). The strains could tolerate and grow in cyanide concentrations of up to 5 mM. Increased cyanide levels in the media caused an extension of lag phase in the bacterial growth indicating that they need some period of acclimatisation. The rate of cyanide removal by the strains depends on the initial cyanide and glucose concentrations. When initial cyanide and glucose concentrations were increased up to 5 mM, cyanide removal rate increased up to 63 and 61 per cent by Bacillus pumilus and Pseudomonas putida. Metabolic products such as ammonia and formate were detected in culture supernatants, suggesting a direct hydrolytic pathway without an intermediate formamide. The study clearly demonstrates the potential of aerobic treatment with cyanide degrading bacteria for cyanide removal in cassava factory wastewaters.

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Descritores: Biodegradação Ambiental
Bacillus/metabolismo
Cianetos/metabolismo
Pseudomonas putida/metabolismo
Águas Residuárias/química
-Amônia/metabolismo
Bacillus/isolamento & purificação
Reatores Biológicos/microbiologia
Formiatos/metabolismo
Glucose/metabolismo
Índia
Manihot
Pseudomonas putida/isolamento & purificação
/genética
RNA, RIBOSOMAL, ABNORMALITIES, MULTIPLES/genética
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-755803
Autor: Luo, Qun; He, Ying; Hou, Deng-Yong; Zhang, Jian-Guo; Shen, Xian-Rong.
Título: GPo1 alkB gene expression for improvement of the degradation of diesel oil by a bacterial consortium
Fonte: Braz. j. microbiol;46(3):649-657, July-Sept. 2015. tab, ilus.
Idioma: en.
Projeto: Shanghai Natural Science Foundation of China.
Resumo:

To facilitate the biodegradation of diesel oil, an oil biodegradation bacterial consortium was constructed. The alkane hydroxylase (alkB) gene of Pseudomonas putida GPo1 was constructed in a pCom8 expression vector, and the pCom8-GPo1 alkB plasmid was transformed into Escherichia coli DH5α. The AlkB protein was expressed by diesel oil induction and detected through SDS-polyacrylamide gel electrophoresis. The culture of the recombinant (pCom8-GPo1 alkB/E. coli DH5α) with the oil biodegradation bacterial consortium increased the degradation ratio of diesel oil at 24 h from 31% to 50%, and the facilitation rates were increased as the proportion of pCom8-GPo1 alkB/E. coli DH5α to the consortium increased. The results suggested that the expression of the GPo1 gene in E. coli DH5α could enhance the function of diesel oil degradation by the bacterial consortium.

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Descritores: Acinetobacter/metabolismo
Biodegradação Ambiental
/genética
CYTOCHROME P-ALDEHYDES CYPABBREVIATIONS AS TOPICA/genética
Escherichia coli/metabolismo
Consórcios Microbianos/genética
Organismos Geneticamente Modificados/metabolismo
Pseudomonas putida/enzimologia
-Acinetobacter/genética
Escherichia coli/enzimologia
Escherichia coli/genética
Óleos Combustíveis
Gasolina
Engenharia Genética
Oxirredução
Organismos Geneticamente Modificados/genética
Plasmídeos/genética
Pseudomonas putida/genética
Pseudomonas putida/metabolismo
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-741280
Autor: Yu, Fang-Bo; Li, Xiao-Dan; Ali, Shinawar Waseem; Shan, Sheng-Dao; Luo, Lin-Ping; Guan, Li-Bo.
Título: Further characterization of o-nitrobenzaldehyde degrading bacterium Pseudomonas sp: ONBA-17 and deduction on its metabolic pathway
Fonte: Braz. j. microbiol;45(4):1303-1308, Oct.-Dec. 2014. graf, tab.
Idioma: en.
Projeto: China National Natural Science Foundation. Agricultural and Forestry Carbon Sinks and Ecological Environmental Remediation; . Zhejiang Provincial Natural Science Foundation; . Qingnianbajian Program of Zhejiang Agricultural and Forestry University.
Resumo: A previously reported o-nitrobenzaldehyde (ONBA) degrading bacterium Pseudomonas sp. ONBA-17 was further identified and characterized. Based on results of DNA base composition and DNA-DNA hybridization, the strain was identified as P. putida. Its degradation effect enhanced with increase of inoculum amount and no lag phase was observed. Higher removal rate was achieved under shaking conditions. All tested ONBA with different initial concentrations could be completely degraded within 5 d. In addition, degradative enzyme(s) involved was confirmed as intra-cellular distributed and constitutively expressed. Effects of different compounds on relative activity of degradative enzyme(s) within cell-free extract were also evaluated. Finally, 2-nitrobenzoic acid and 2, 3-dihydroxybenzoic acid were detected as metabolites of ONBA degradation by P. putida ONBA-17, and relevant metabolic pathway was preliminary proposed. This study might help with future research in better understanding of nitroaromatics biodegradation.
Descritores: Benzaldeídos/metabolismo
Redes e Vias Metabólicas
Pseudomonas putida/metabolismo
-Biotransformação
Hidroxibenzoatos/metabolismo
Nitrobenzoatos/metabolismo
Pseudomonas putida/classificação
Pseudomonas putida/genética
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-741272
Autor: Peng, Yanjie; He, Yanhui; Wu, Zhansheng; Lu, Jianjiang; Li, Chun.
Título: Screening and optimization of low-cost medium for Pseudomonas putida Rs-198 culture using RSM
Fonte: Braz. j. microbiol;45(4):1229-1237, Oct.-Dec. 2014. graf, tab.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Science and Technology Fund Projects of Shihezi University.
Resumo: The plant growth-promoting rhizobacterial strain Pseudomonas putida Rs-198 was isolated from salinized soils from Xinjiang Province. We optimized the composition of the low-cost medium of P. putida Rs-198 based on its bacterial concentration, as well as its phosphate-dissolving and indole acetic acid (IAA)-producing capabilities using the response surface methodology (RSM), and a mathematical model was developed to show the effect of each medium component and its interactions on phosphate dissolution and IAA production. The model predicted a maximum phosphate concentration in medium containing 63.23 mg/L inorganic phosphate with 49.22 g/L corn flour, 14.63 g/L soybean meal, 2.03 g/L K2HPO4, 0.19 g/L MnSO4 and 5.00 g/L NaCl. The maximum IAA concentration (18.73 mg/L) was predicted in medium containing 52.41 g/L corn flour, 15.82 g/L soybean meal, 2.40 g/L K2HPO4, 0.17 g/L MnSO4 and 5.00 g/L NaCl. These predicted values were also verified through experiments, with a cell density of 10(13) cfu/mL, phosphate dissolution of 64.33 mg/L, and IAA concentration of 18.08 mg/L. The excellent correlation between predicted and measured values of each model justifies the validity of both the response models. The study aims to provide a basis for industrialized fermentation using P. putida Rs-198.
Descritores: Meios de Cultura/química
Pseudomonas putida/crescimento & desenvolvimento
-Carga Bacteriana
Custos e Análise de Custo
Meios de Cultura/economia
Ácidos Indolacéticos/metabolismo
Programas de Rastreamento
Modelos Teóricos
Fosfatos/metabolismo
Pseudomonas putida/metabolismo
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  8 / 17 LILACS  
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Id: lil-733352
Autor: Riojas-Rodríguez, Horacio; Álamo-Hernández, Urinda; Texcalac-Sangrador, José Luis; Romieu, Isabelle.
Título: Health impact assessment of decreases in PM10 and ozone concentrations in the Mexico City Metropolitan Area: A basis for a new air quality management program / Evaluación de impacto en salud ante reducciones de PM10 y ozono en la Zona Metropolitana del Valle de México: Base para un nuevo programa de calidad del aire
Fonte: Salud pública Méx;56(6):579-591, nov.-dic. 2014. ilus, tab.
Idioma: en.
Resumo: Objective. To conduct a health impact assessment (HIA) to quantify health benefits for several PM and O3 air pollution reduction scenarios in the Mexico City Metropolitan Area (MCMA). Results from this HIA will contribute to the scientific support of the MCMA air quality management plan (PROAIRE) for the period 2011-2020. Materials and methods. The HIA methodology consisted of four steps: 1) selection of the air pollution reduction scenarios, 2) identification of the at-risk population and health outcomes for the 2005 baseline scenario, 3) selection of concentration-response functions and 4) estimation of health impacts. Results. Reductions of PM10 levels to 20 μg/m³ and O3 levels to 0.050ppm (98 µg/m³) would prevent 2300 and 400 annual deaths respectively. The greatest health impact was seen in the over-65 age group and in mortality due to cardiopulmonary and cardiovascular disease. Conclusion. Improved air quality in the MCMA could provide significant health benefits through focusing interventions by exposure zones.

Objetivo. Realizar una evaluación de impacto en salud (EIS) que documente los beneficios en salud ante diversos escenarios de reducción de PM10 y O3 en el aire de la Zona Metropolitana del Valle de México (ZMVM). Los resultados contribuyen al sustento científico del plan de gestión de calidad del aire (PROAIRE 2011-2020). Material y métodos. La metodología de EIS comprende cuatro pasos: 1) selección de los escenarios de reducción, 2) identificación de la población en riesgo y de los eventos en salud para el año basal 2005, 3) selección de las funciones de concentración-respuesta y 4) estimación del impacto en la salud. Resultados. Reducciones de PM10 a 20μg/m³ y de O3 a 0.050ppm (98 µg/m³) evitarían, respectivamente, cerca de 2 300 y 400 muertes por año. El mayor impacto se observa en el grupo de más de 65 años y en la mortalidad por causas cardiopulmonares y cardiovasculares. Conclusiones. Mejorar la calidad del aire en la ZMVM podría reflejar importantes beneficios para la salud focalizados por zonas o áreas de exposición.
Descritores: Pseudomonas putida/metabolismo
Estirenos/metabolismo
-Aldeído Oxirredutases/metabolismo
Biodegradação Ambiental
Proteínas de Escherichia coli
Compostos de Epóxi/metabolismo
Ácido Glutâmico/metabolismo
Isomerases/metabolismo
Oxirredução
Consumo de Oxigênio
Fenilacetatos/metabolismo
Pseudomonas putida/enzimologia
Pseudomonas putida/crescimento & desenvolvimento
Estireno
Ácido Succínico
Succinatos/metabolismo
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-733293
Autor: Pozzio, María.
Título: "El hecho de que sean más mujeres, no garantiza nada": feminización y experiencias de las mujeres en la ginecobstetricia en México / "The fact that there are more women doesn't guarantee anything": The feminization of obstetrics and gynecology and the experiences of female medical professionals in Mexico
Fonte: Salud colect;10(3):325-337, sep.-dic. 2014. tab.
Idioma: es.
Resumo: En el marco de la creciente feminización de la profesión médica en México, el artículo indaga sobre las características de este proceso para el caso de la ginecobstetricia. Considerando la feminización como un proceso de cambio, que se analiza cuantitativa y cualitativamente, el artículo se detiene en especial en las experiencias de las mujeres ginecobstetras, experiencias que se dan en el seno de una especialidad que, desde sus orígenes, funcionó como un dispositivo de control del cuerpo de las mujeres. Basado en una investigación etnográfica, el artículo combina fuentes estadísticas, de archivo y de observación de campo. El material que surge de las entrevistas muestra las experiencias y tensiones que viven las ginecobstetras en este contexto.

In the framework of an increasing feminization of the medical profession in Mexico, this article explores the characteristics of this process in the obstetrics and gynecology specialty. Understanding feminization as a process of change to be analyzed both quantitatively and qualitatively, the article focuses special attention on the experiences of female obstetrician-gynecologists within a medical specialty that has since its origins functioned as a mechanism of control over women's bodies. Based on ethnographic research, the article combines statistical and archival sources and field observation. The interviews reveal the experiences and tensions women obstetrician-gynecologists encounter in this context.
Descritores: Oxirredutases do Álcool/química
Oxirredutases do Álcool/metabolismo
Arginina/química
Pseudomonas putida/enzimologia
-Oxirredutases do Álcool/genética
Oxirredutases do Álcool/isolamento & purificação
Ligação Competitiva/genética
Catálise
Ativação Enzimática/genética
Mononucleotídeo de Flavina/metabolismo
Cinética
Ligantes
Mutagênese Sítio-Dirigida
Ácidos Mandélicos/metabolismo
Fenilacetatos/metabolismo
Ligação Proteica/genética
Pseudomonas putida/genética
Especificidade por Substrato/genética
Sulfitos/metabolismo
Tipo de Publ: Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.
Responsável: BR1.1 - BIREME


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Id: lil-731050
Autor: Mello, Victor Villaça Cardoso de; Barbosa, André Cavalcante da Silva; Morais, Mariana Pacheco Lima de Assis; Gomes, Simone Guimarães Farias; Vasconcelos, Márcia Maria Vendiciano Barbosa; Caldas Júnior, Arnaldo de França.
Título: Temporomandibular Disorders in a Sample Population of the Brazilian Northeast
Fonte: Braz. dent. j;25(5):442-446, Sep-Oct/2014. tab, graf.
Idioma: en.
Resumo: Temporomandibular disorder (TMD) is a common condition. This study is part of a research group and it investigated the prevalence of TMD and myofascial pain and its association with gender, age and socioeconomic class. The sample comprised 100 subjects, aged 15 to 70, users of the Family Health Units' services, in the city of Recife, PE, Brazil. The TMD degree was evaluated using the Research Diagnostic Criteria for TMD and socioeconomic class by the Economic Classification Criteria Brazil. Categorical variables were analyzed by chi-square test for proportions and Fisher's exact test for 2x2 tables, and binary logistic analysis to track the relationship between the independent and dependent variables. According to the results, 42% of the subjects had TMD and 14% myofascial pain. No statistically significant association could be found between TMD and gender or socioeconomic class, but it was found to have statistically significant association with age, and myofascial pain was associated with socioeconomic class. Considering that the results of the present study should be confirmed by further studies and the fact that this was a pilot study, the prevalence must be analyzed with caution.

Disfunção temporomandibular (DTM) é uma condição comum. Este estudo é parte de um grupo de pesquisa e investigou a prevalência de DTM e dor miofascial e suas associações com sexo, idade e classe socioeconômica. A amostra foi composta por 100 indivíduos, com idades entre 15 e 70 anos, usuários das Unidades de Saúde da Família, na cidade de Recife, PE. O grau de DTM foi avaliado usando os Critérios de Diagnósticos Científicos em DTM, e classe socioeconômica com o Critério de Classificação Econômica Brasil. As variáveis categóricas foram analisadas pelo teste do qui-quadrado para proporções e teste exato de Fisher para tabelas 2x2, e a análise logística binária para traçar a relação entre as variáveis independentes e dependentes. De acordo com os resultados, 42% dos indivíduos tinham DTM e 14% dor miofascial. Não houve associação estatisticamente significativa entre DTM e sexo ou classe socioeconômica, mas houve associação estatisticamente significativa com a idade e a dor miofascial foi associada com a classe socioeconômica. Considerando-se que os resultados do presente estudo devam ser confirmados em outros estudos e por causa de sua natureza piloto, a prevalência deve ser analisada com cautela.
Descritores: Regulação Bacteriana da Expressão Gênica
Óperon
Fenilacetatos/metabolismo
Pseudomonas putida/genética
Pseudomonas putida/crescimento & desenvolvimento
-Técnicas de Cultura de Células
Divisão Celular
Meios de Cultura
Carbono/metabolismo
Coenzima A Ligases/biossíntese
Coenzima A Ligases/genética
Compostos Inorgânicos/metabolismo
Oxigenases/biossíntese
Oxigenases/genética
Pseudomonas putida/metabolismo
Reprodutibilidade dos Testes
Ácido Succínico/metabolismo
Ativação Transcricional
Responsável: BR1.1 - BIREME



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