Base de dados : LILACS
Pesquisa : B03.440.400.425.875 [Categoria DeCS]
Referências encontradas : 4 [refinar]
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Id: lil-622797
Autor: Mandelli, Fernanda; Yamashita, Fábio; Pereira, José L; Mercadante, Adriana Z.
Título: Evaluation of biomass production, carotenoid level and antioxidant capacity produced by Thermus filiformis using fractional factorial design
Fonte: Braz. j. microbiol;43(1):126-134, Jan.-Mar. 2012. ilus, tab.
Idioma: en.
Resumo: A fractional factorial design 2(5-1) was used to evaluate the effect of temperature, pH, and concentrations of yeast extract, tryptone and Nitsch's trace elements on the biomass, total carotenoids and protection against singlet oxygen by carotenoid extracts of the bacterium Thermus filiformis. In addition, the carotenoid composition was determined by high-performance liquid chromatography connected to a diode array and mass spectrometer detectors (HPLC-DAD-MS/MS). The production of biomass ranged from 0.113 to 0.658 g/L, the total carotenoid from 137.6 to 1,517.4 mg/g and the protection against singlet oxygen from 4.3 to 85.1 %. Results of the fractional factorial design showed that temperature had a negative effect on biomass production and a positive effect on carotenoid content and protection against singlet oxygen, besides, high levels of pH value, concentrations of yeast extract and tryptone had a positive effect on biomass production only at lower temperatures. The main carotenoids of T. filiformis were thermozeaxanthins. In the tested conditions, changes in the levels of the variables influenced the biomass, carotenoid production, and protection against singlet oxygen, although they did not influence the carotenoid profile. The results of this study provide a better understanding on the interactions among certain nutritional and cultivation conditions of a thermophile bacterium, Thermus filiformis, on biomass and carotenoid amounts, as well as on the antioxidant capacity.
Descritores: Biomassa
Cromatografia Líquida
Carotenoides/análise
Iodoperaceto/análise
Oxigênio Singlete/análise
Thermus/genética
Thermus/isolamento & purificação
Leveduras
-Técnicas de Química Combinatória
Concentração de Íons de Hidrogênio
Métodos
Tipo de Publ: Estudo Comparativo
Estudo de Avaliação
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica


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Id: lil-508482
Autor: Pérez, Fernando; Baridón, María de los Ángeles; Henen, Jaime; Venegoni, Graciela.
Título: Obtención de ADN polimerasa de Thermus aquaticus en un laboratorio de mediana complejidad / Thermus aquaticus polymerase DNA's production in a medium complexity laboratory
Fonte: Acta bioquím. clín. latinoam;40(4):521-524, dic. 2006.
Idioma: es.
Resumo: La PCR (reaccion en cadena de la polimerasa) es una técnica de diagnóstico molecular muy sensible, capaz de revelar tan sólo una copia de ADN en una mexcla compleja del mismo. El punto central para la difusión de esta metodología fue el descubrimiento de una ADN polimerasa estable al calor aislada de Thermus aquaticus (taq ADN pol), lo cual permitió automatizar el proceso de PCR usando un ciclador térmico, sin la necesidad de una adición continua de ADN polimerasa fresca. El objetivo del trabajo fue adaptar un método para la obtención de taq ADN pol en un laboratorio hospitalario de mediana complejidad usando el equipamiento y los medios de cultivo disponibles. Con este mètodo se consiguió producir un extracto crudo de 10.000 unidades de enzima, de fácil purificación, que mostró tener muy buena actividad enzimática por lo cual es posible utilizarla en una amplia variedad de aplicaciones (PCR clásica, PCR anidada, PCR transcriptasa reversa). La actividad enzimática para estos casos fue comparable con la de la taq polimerasa que se adquiere en el mercado.
Descritores: Reação em Cadeia da Polimerase
Taq Polimerase
Thermus
-Técnicas de Laboratório Clínico
Laboratórios
Responsável: AR144.1 - CIBCHACO - Centro de Información Biomedica del Chaco


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Id: lil-445755
Autor: Kotsias, Basilio A.
Título: Maravillas del infierno / The wonders of hell
Fonte: Medicina (B.Aires);65(5):461-464, 2005. ilus.
Idioma: es.
Descritores: Aeropyrum/enzimologia
Fontes Termais/microbiologia
Thermus/enzimologia
-Aeropyrum/isolamento & purificação
Canais de Potássio/genética
Parede Celular/química
Taq Polimerase/genética
Thermus/isolamento & purificação
Limites: Animais
Responsável: BR1.1 - BIREME


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Id: lil-223982
Autor: Diaz, R. S; Sabino, E. C.
Título: Accuracy of replication in the polymerase chain reaction. Comparison between Thermotoga maritima DNA polymerase and Thermus aquaticus DNA polymerase
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;31(10):1239-42, Oct. 1998. tab.
Idioma: en.
Resumo: For certain applications of the polymerase chain reaction (PCR), it may be necessary to consider the accuracy of replication. The breakthrough that made PCR user friendly was the commercialization of Thermus aquaticus (Taq) DNA polymerase, an enzyme that would survive the high temperatures needed for DNA denaturation. The development of enzymes with an inherent 3' to 5' exonuclease proofreading activity, lacking in Taq polymerase, would be an improvement when higher fidelity is needed. We used the forward mutation assay to compare the fidelity of Taq polymerase and Thermotoga maritima (ULTMATM) DNA polymerase, an enzyme that does have proofreading activity. We did not find significant differences in the fidelity of either enzyme, even when using optimal buffer conditions, thermal cycling parameters, and number of cycles (0.2 per cent and 0.13 per cent error rates for ULTMATM and Taq, respectively, after reading about 3,000 bases each). We conclude that for sequencing purposes there is no difference in using a DNA polymerase that contains an inherent 3' to 5' exonuclease activity for DNA amplification. Perhaps the specificity and fidelity of PCR are complex issues influenced by the nature of the target sequence, as well as by each PCR component.
Descritores: Replicação do DNA
DNA Polimerase Dirigida por DNA
Reação em Cadeia da Polimerase
Thermotoga maritima/enzimologia
Thermotoga maritima/genética
Thermus/enzimologia
Thermus/genética
Tipo de Publ: Revisão
Estudo Comparativo
Responsável: BR1.1 - BIREME



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