Base de dados : LILACS
Pesquisa : B03.440.400.425.967.750 [Categoria DeCS]
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Texto completo SciELO Brasil
Melo, Itamar Soares de
Texto completo
Id: lil-723127
Autor: Kavamura, Vanessa Nessner; Melo, Itamar Soares de.
Título: Effects of different osmolarities on bacterial biofilm formation
Fonte: Braz. j. microbiol;45(2):627-631, Apr.-June 2014. ilus, graf, tab.
Idioma: en.
Resumo: Biofilm formation depends on several factors. The influence of different osmolarities on bacterial biofilm formation was studied. Two strains (Enterobacter sp. and Stenotrophomonas sp.) exhibited the most remarkable alterations. Biofilm formation is an important trait and its use has been associated to the protection of organisms against environmental stresses.
Descritores: Biofilmes/efeitos dos fármacos
Enterobacter/efeitos dos fármacos
Stenotrophomonas/efeitos dos fármacos
-Concentração Osmolar
Cloreto de Sódio/metabolismo
Responsável: BR1.1 - BIREME

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Texto completo SciELO Brasil
Texto completo
Id: lil-592180
Autor: Ramos, Patrícia Locosque; Moreira-Filho, Carlos Alberto; Van Trappen, Stefanie; Swings, Jean; Vos, Paul De; Barbosa, Heloiza Ramos; Thompson, Cristiane Carneiro; Vasconcelos, Ana Tereza Ribeiro; Thompson, Fabiano Lopes.
Título: An MLSA-based online scheme for the rapid identification of Stenotrophomonas isolates
Fonte: Mem. Inst. Oswaldo Cruz;106(4):394-399, June 2011. ilus, tab.
Idioma: en.
Projeto: CNPq; . FAPESP; . CNPq; . IFS; . FAPESP.
Resumo: An online scheme to assign Stenotrophomonas isolates to genomic groups was developed using the multilocus sequence analysis (MLSA), which is based on the DNA sequencing of selected fragments of the housekeeping genes ATP synthase alpha subunit (atpA), the recombination repair protein (recA), the RNA polymerase alpha subunit (rpoA) and the excision repair beta subunit (uvrB). This MLSA-based scheme was validated using eight of the 10 Stenotrophomonas species that have been previously described. The environmental and nosocomial Stenotrophomonas strains were characterised using MLSA, 16S rRNA sequencing and DNA-DNA hybridisation (DDH) analyses. Strains of the same species were found to have greater than 95 percent concatenated sequence similarity and specific strains formed cohesive readily recognisable phylogenetic groups. Therefore, MLSA appeared to be an effective alternative methodology to amplified fragment length polymorphism fingerprint and DDH techniques. Strains of Stenotrophomonas can be readily assigned through the open database resource that was developed in the current study (
Descritores: DNA Bacteriano
Tipagem de Sequências Multilocus/métodos
-Técnicas de Tipagem Bacteriana
RNA Polimerases Dirigidas por DNA
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME

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Texto completo SciELO Brasil
Texto completo
Id: lil-571372
Autor: Yang, Hongmei; Lou, Kai.
Título: Succession and growth strategy of a spring microbial community from kezhou sinter in China
Fonte: Braz. j. microbiol;42(1):41-45, Jan.-Mar. 2011. ilus.
Idioma: en.
Projeto: Open Project Program of the Specific Habitat Microorganisms Laboratory of Xinjiang; . 973 Pre-research Program of China.
Resumo: The succession and growth strategy of a spring microbial community under earthquake action were investigated. The majority of pre-earthquake isolates belonged to the Gammaproteobacteria, including two numerically dominant Stenotrophomonas sp. RB25 and Acinetobacter sp. RB11 (r-strategists). The predominant post-earthquake isolates were Alphaproteobacteria, with Rhizobium sp. RA42 (K-strategists) being dominant among these organisms.
Descritores: Crescimento Bacteriano
Microbiologia Ambiental
Infecções por Bactérias Gram-Negativas
Gammaproteobacteria/isolamento & purificação
Rhizobium/isolamento & purificação
Stenotrophomonas/isolamento & purificação
Tipo de Publ: Relatório Técnico
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica

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Texto completo SciELO Chile
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Id: lil-538011
Autor: Hernández García, Marcela; Morgante, Verónica; Ávila Perez, Marcela; Villalobos Biaggini, Patricio; Miralles Noé, Pola; González Vergara, Myriam; Seeger Pfeiffer, Michael.
Título: Novel s-triazine-degrading bacteria isolated from agricultural soils of central Chile for herbicide bioremediation
Fonte: Electron. j. biotechnol;11(5):5-6, Dec. 2008. ilus, tab.
Idioma: en.
Projeto: Millennium Nucleus.
Resumo: s-Triazine-degrading bacterial strains were isolated from long-term simazine-treated agricultural soils of central Chile. The number of culturable heterotrophic bacteria of these agricultural soils (7 x 10(6) CFU/g of dry soil) was not affected by simazine application on field. The simazine-degrading bacterial strains P51, P52 and C53 were isolated by enrichment in minimal medium using simazine as the sole nitrogen source. Resting cells of strains P51 and P52 degraded >80 percent of simazine within 48 hrs, whereas strain C53 was able to remove >60 percent of the herbicide. The atzA and atzD genes of the s-triazine upper and lower catabolic pathways were detected in strains P51 and C53, while only atzD gene was observed in strain P52. To compare the bacterial 16S rRNA gene sequence structure, ARDRA were performed using the restriction enzymes Msp1 and Hha1. ARDRA indicated that strain P52 was a different ribotype than C53 and P51 strains. For further characterization the novel isolates were identified by 16S rRNA gene sequencing. Strains C53 and P51 belong to the genus Stenotrophomonas and the strain P52 belongs to the genus Arthrobacter . s -Triazine-degrading bacterial strains isolated from contaminated soils could be used as biocatalysts for bioremediation of these herbicides.
Descritores: Simazina/administração & dosagem
Simazina/uso terapêutico
Triazinas/administração & dosagem
Triazinas/uso terapêutico
-Cultivos Agrícolas
Biodegradação Ambiental
Herbicidas/administração & dosagem
Herbicidas/uso terapêutico
Responsável: CL1.1 - Biblioteca Central

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Texto completo SciELO Brasil
Timenetsky, Jorge
Martinez, Marina Baquerizo
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Id: lil-330263
Autor: Garcia, Doroti de Oliveira; Timenetsky, Jorge; Martinez, Marina Baquerizo; Francisco, Waldemar; Sinto, Sumiko I; Yanaguita, Roberto Mitio.
Título: Proteases (caseinase and elastase), hemolysins, adhesion and susceptibility to antimicrobials of Stenotrophomonas maltophilia isolates obtained from clinical specimens
Fonte: Braz. j. microbiol;33(2):157-162, Apr.-Jun. 2002. ilus, tab.
Idioma: en.
Resumo: Forty-six S. maltophilia isolates obtained from hospital clinical specimens were studied for protease (caseinase and elastase) production, hemolytic activity, adhesion to HEp-2 cells, plastic and glass. Susceptibility to antimicrobial agents was also evaluated. The majority of isolates were obtained from respiratory tract secretions of patients using medical devices. All the isolates grown overnight were able to hydrolyze casein at 30ºC and 37ºC. After 72h, all the isolates hydrolyzed elastase at 30ºC and 40 isolates (87 per cent) at 37ºC. Most of the isolates presented hemolytic activity after 96h of incubation at both temperatures. Rabbit blood showed the hightest hemolytic activity, after 96h 61(per cent) and 98(per cent) of tested isolates presented b-hemolysis at 30ºC and 37ºC, respectively. All isolates were susceptible to trimethoprim-sulfametoxazole and were resistant to most b-lactams tested. By the dilution method, S. maltophilia showed a high susceptibility to ticarcillin-clavulanate and a lower susceptibility to ciprofloxacin than the agar diffusion. The isolates showed adhesion to HEp-2 cells, plastic and glass. The proteolytic activities and adhesion to inanimate surfaces detected in S. maltophilia can be related to the pathogenesis of this bacterium and/or medical device colonization which favors the development of nosocomial infections.
Descritores: Endopeptidases
Proteínas Hemolisinas
Análise Química do Sangue
Meios de Cultura
Limites: Humanos
Tipo de Publ: Revisão de Integridade Científica
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica

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