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Id: biblio-961295
Autor: Espino, Danamirys; Sosa Díaz, Janice; Sosa Díaz, Regina Yamilet.
Título: Klebsiella pneumoniae, un patógeno de alta prioridad para la fabricaciòn de nuevos antibióticos / Klebsiella pneumoniae, a high priority pathogen for producing new antibiotics
Fonte: Rev. medica electron;40(4):1271-1273, jul.-ago. 2018. ilus.
Idioma: es.
Descritores: Klebsiella pneumoniae/patogenicidade
Antibacterianos/farmacologia
-Pacientes
Prática Profissional/normas
Enterobacteriaceae/patogenicidade
Limites: Humanos
Tipo de Publ: Carta
Responsável: CU424.1 - Centro Provincial de Información de Ciencias Médicas


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Id: biblio-1131473
Autor: Geron, C. C; Ribeiro, C. G. G; Kobayashi, R. K. T; Nakazato, G.
Título: Classificação dos graus de lesões de aerossaculite em perus associadas com enterobactérias / [Classification of the levels of the airsacculitis injuries in turkeys associated with Enterobacteriaceae]
Fonte: Arq. bras. med. vet. zootec. (Online);72(4):1277-1285, July-Aug. 2020. tab, ilus.
Idioma: pt.
Resumo: Foram padronizados os graus de lesões dos sacos aéreos em perus com aerossaculite, associadas com a presença de isolados de enterobactérias nesses órgãos. Um total de 110 amostras de sacos aéreos de perus machos com aerossaculite foi coletado para o estudo. Durante o processo de abate, as amostras foram coletadas por meio de swabs e submetidas a três métodos de armazenamento (imediato, congelado ou pré-incubado após congelamento) para posterior comparação das suas eficiências de isolamento. Os gêneros da família Enterobacteriaceae foram identificados pelas séries bioquímicas EPM, MILi e citrato de Simmons. O crescimento bacteriano ocorreu em 43,64% das amostras. Neste estudo, quatro padrões de lesões de aerossaculite foram identificados de acordo com as características patológicas dos sacos aéreos. Os principais gêneros de enterobactérias identificadas foram: Escherichia coli, Citrobacter, Proteus, Edwardsiella, Morganella, Kluyvera, Salmonella e Klebsiella. Foi observado que os graus padronizados como 3 e 4 apresentaram maior variedade de gêneros bacterianos. O armazenamento imediato apresentou maior porcentagem de positividade, 41,82%, no entanto o pré-incubado após congelamento se apresentou mais eficaz em relação à quantidade de colônias.(AU)

The degrees of air sac lesions in turkeys with airsacculitis were standardized, associated with the presence of Enterobacteriaceae isolated from these organs. A total of 110 samples of air sacs from male turkeys with airsacculitis were collected and analyzed. During the slaughtering process, the sample collection was done using swabs and submitted to three storage methods (immediate, frozen, or pre incubated after freezing) for further comparison of their isolated efficiency. The bacterial genera of the family Enterobacteriaceae were identified biochemical series EPM, MILi and Simmons citrate. Bacterial growth occurred in 43.64% of samples. In this study, four patterns of aerossaculitis lesions were identified according to the pathological characteristics of air sacs. The frequencies of the Enterobacteriaceae isolated identified in the samples were: Escherichia coli, Citrobacter, Proteus, Edwardsiella, Morganell, Kluyvera, Salmonella and Klebsiella. Otherwise, it was observed that the levels already standardized as level three and four showed higher variety of genus. The immediate storage showed higher percentage of positivity at 41.82%, however, the pre incubated after freezing showed more efficiency in relation to the quantity of colonies.(AU)
Descritores: Perus
Sacos Aéreos/microbiologia
Enterobacteriaceae/isolamento & purificação
Infecções por Enterobacteriaceae/veterinária
-Proteus
Salmonella
Citrobacter
Edwardsiella
Morganella
Kluyvera
Escherichia coli
Klebsiella
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: biblio-1099651
Autor: Morales Urresta, Erika Estefanía; Velasco Polo, Verónica Alexandra; Cárdenas Martínez, Ana Gabriela; Oñate Araque, Ximena Lucia; Núñez Freile, Byron Fabián; Guerrero Toapanta, Fausto Marcos; Reyes Chacón, Jorge Aníbal.
Título: Carbapenemasas y sensibilidad a los antibióticos no ß-lactámicos en Klebsiella pneumoniae resistente a los carbapenémicos en un hospital de tercer nivel de complejidad. Quito ­ Ecuador / Carbapenemases and sensitivity to non-ß-lactam antibiotics in Klebsiella pneumoniae resistant to carbapenems in a third level hospital. Quito - Ecuador
Fonte: Cambios rev. méd;18(2):52-57, 2019/12/27. tabs..
Idioma: es.
Resumo: INTRODUCCIÓN. La resistencia de Klebsiella pneumoniae a carbapenémicos ha aumentado con los años, reduciendo opciones terapéuticas. Puede deberse a dos mecanismos principales, como: la producción de carbapenemasas y alteración de la permeabilidad de la membrana. OBJETIVO. Analizar la frecuencia de Klebsiella pneumoniae resistente a carbapenémicos, junto al mecanismo de resistencia informado por el Laboratorio Nacional de Referencia y la sensibilidad a antibióticos usados para uso terapéutico. MATERIA-LES Y MÉTODOS. Estudio retrospectivo, de población y muestra conocida. Se determinó la sensibilidad/resistencia de Klebsiella pneumoniae: todos los datos aislados de Klebsiella pneumoniae. Se reportaron 11 809 bacilos gram negativos pertenecientes a la familia Enterobacteriaceae. Se utilizó el sistema Whonet 5.6 2017 y BacLink2, así como la revisión de los resultados enviados al Centro Nacional de Referencia para la Resistencia a los Antimicrobianos del Instituto Nacional de Investigación de Salud Pública Izquieta Pérez para investigación de carbapenemasas en el Hospital de Especialidades Carlos Andrade Marín,en el período abril 2016 a mayo 2018. RESULTADOS. El 20,5% (2 421; 11 809) correspondieron a Klebsiella pneumoniae y de estos, 32,9% (797; 2 421) mostraron resistencia a Meropenem. Existió mayor frecuencia en muestra de orina, secreción, sangre y aspirado traqueal. Predominó en varones de más de 61 años. Se clasificó en 15 grupos fenotípicos según los perfiles de resistencia a los antibióticos utilizados como alternativa terapéutica. CONCLUSIÓN. La presencia de Klebsiella pneumoniae produjo una carbapenemasa resistente a los medi-camentos utilizados como tratamiento, llevó a pensar en el uso de otros medicamentos como: fosfomicina o ceftazidima/viabactam; sin embargo, se desarrolló medidas de control y prevención de infecciones, así como programas para el uso de antibióticos.

INTRODUCTION. The resistance of Klebsiella pneumoniae to carbapenems has increased over the years, reducing therapeutic options. It can be due to two main mechanisms, such as: the production of carbapene-mases and alteration of membrane permeability. OBJECTIVE. Analyse the frequency of carbapenem-resistant Klebsiella pneumoniae, along with the resistance mechanism reported by the National Reference Laboratory and sensitivity to antibiotics used for therapeutic use. MATERIALS AND METHODS. Retrospective study, po-pulation and known sample. The sensitivity / resistance of Klebsiella pneumoniae was determined: all isolated were Klebsiella pneumoniae. 11 809 gram negative bacillus belonging to the Enterobacteriaceae family were reported. The Whonet 5.6 2017 and BacLink2 system was used, as well as the review of the results sent to the National Reference Center for the Antimicrobial Resistance of the National Institute of Public Health Research Izquieta Pérez for carbapenemases research at the Carlos Andrade Marín Speciality Hospital, in the period april 2016 to may 2018. RESULTS. 20,5% (2 421; 11 809) were Klebsiella pneumoniae and of these, 32,9% (797; 2 421) showed resistance to Meropenem. There was a higher frequency in urine sample, secretion, blood and tracheal aspiration. Predominance in males over 61 years old. It was classified into 15 phenotypic groups according to antibiotic resistance profiles used as a therapeutic alternative. CONCLUSION. The pre-sence of Klebsiella pneumoniae produced a carbapenemase drug-resistance used as treatment, leading to thought on the use of other medicines such as phosphomycin or ceftazidima/avibactam; however, infection control and prevention measures were developed, as well as programs for the use of antibiotics.
Descritores: Fenótipo
Carbapenêmicos
beta-Lactamas
Enterobacteriaceae
Klebsiella pneumoniae
Antibacterianos
-Terapêutica
Fatores R
Testes de Sensibilidade Microbiana
Gestão de Antimicrobianos
Unidades de Terapia Intensiva
Microbiologia
Limites: Humanos
Masculino
Feminino
Tipo de Publ: Estudo de Validação
Responsável: EC162.1


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Id: biblio-1039180
Autor: Rossi, Flávia; Girardello, Raquel; Cury, Ana Paula; Di Gioia, Thais Sabato Romano; Almeida Jr, João Nóbrega de; Duarte, Alberto José da Silva.
Título: Emergence of colistin resistance in the largest university hospital complex of São Paulo, Brazil, over five years
Fonte: Braz. j. infect. dis;21(1):98-101, Jan.-Feb. 2017. tab.
Idioma: en.
Resumo: Abstract Colistin resistance involving Gram-negative bacilli infections is a challenge for health institutions around of the world. Carbapenem-resistance among these isolates makes colistin the last therapeutic option for this treatment. Colistin resistance among Enterobacteriaceae, Acinetobacter spp., and Pseudomonas spp. was evaluated between 2010 and 2014 years, at Hospital das Clínicas, São Paulo, Brazil. Over five years 1346 (4.0%) colistin resistant Gram-negative bacilli were evaluated. Enterobacteriaceae was the most frequent (86.1%) pathogen isolated, followed by Acinetobacter spp. (7.6%), and Pseudomonas spp. (6.3%). By temporal analysis there was a trend for an increase of colistin resistance among Enterobacteriaceae, but not among non-fermentative isolates. Among 1346 colistin resistant isolates, carbapenem susceptibility was observed in 21.5%. Colistin resistance in our hospital has been alarmingly increased among Klebsiella pneumoniae isolates in both KPC positive and negative, thus becoming a therapeutic problem.
Descritores: Pseudomonas/efeitos dos fármacos
Acinetobacter/efeitos dos fármacos
Colistina/farmacologia
Farmacorresistência Bacteriana/efeitos dos fármacos
Enterobacteriaceae/efeitos dos fármacos
Antibacterianos/farmacologia
-Pseudomonas/isolamento & purificação
Fatores de Tempo
Acinetobacter/isolamento & purificação
Brasil
Testes de Sensibilidade Microbiana
Estudos Retrospectivos
Enterobacteriaceae/isolamento & purificação
Hospitais Universitários
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-839195
Autor: Tuğcu, Murat; Ruhi, Caglar; Gokce, Ali M; Kara, Melih; Aksaray, Sebahat.
Título: A case of urinary tract infection caused by Raoultella planticola after a urodynamic study
Fonte: Braz. j. infect. dis;21(2):196-198, Mar.-Apr. 2017. tab.
Idioma: en.
Resumo: Abstract Here we report the case of a patient who developed urinary tract infection after a urodynamic study. The causative agent was Raoultella planticola, a rare opportunistic pathogen that usually invades immunocompromised patients. While a urinary tract infection with R. planticola has been previously described, this is the first report in which an R. planticola infection developed after a urodynamic study. We postulate that the mechanism of infection was direct invasion of the urinary tract from contaminated urodynamic study equipment. Here, we discuss the role played by isotonic solutions in facilitating bacterial reproduction.
Descritores: Infecções Urinárias/microbiologia
Contaminação de Equipamentos
Enterobacteriaceae/isolamento & purificação
Infecções por Enterobacteriaceae/etiologia
-Urodinâmica
Infecções por Enterobacteriaceae/microbiologia
Limites: Humanos
Masculino
Pessoa de Meia-Idade
Tipo de Publ: Relatos de Casos
Responsável: BR1.1 - BIREME


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Id: biblio-888926
Autor: Pfaller, Michael A; Shortridge, Dee; Sader, Helio S; Gales, Ana; Castanheira, Mariana; Flamm, Robert K.
Título: Ceftolozane-tazobactam activity against drug-resistant Enterobacteriaceae and Pseudomonas aeruginosa causing healthcare-associated infections in Latin America: report from an antimicrobial surveillance program (2013-2015)
Fonte: Braz. j. infect. dis;21(6):627-637, Nov.-Dec. 2017. tab.
Idioma: en.
Resumo: ABSTRACT This study evaluated the in vitro activity of ceftolozane-tazobactam and comparator agents tested against Latin American isolates of Enterobacteriaceae and Pseudomonas aeruginosa from patients with health care-associated infections. Ceftolozane-tazobactam is an antipseudomonal cephalosporin combined with a well-established β-lactamase inhibitor.A total of 2415 Gram-negative organisms (537 P. aeruginosa and 1878 Enterobacteriaceae) were consecutively collected in 12 medical centers located in four Latin American countries. The organisms were tested for susceptibility by broth microdilution methods as described by the CLSI M07-A10 document and the results interpreted according to EUCAST and CLSI breakpoint criteria. Results: Ceftolozane-tazobactam (MIC50/90, 0.25/32 µg/mL; 84.2% susceptible) and meropenem (MIC50/90, ≤0.06/0.12 µg/mL; 92.6% susceptible) were the most active compounds tested against Enterobacteriaceae. Among the Enterobacteriaceae isolates tested, 6.6% were carbapenem-resistant Enterobacteriaceae and 26.4% exhibited an extended-spectrum β-lactamase non-carbapenem-resistant phenotype. Whereas ceftolozane-tazobactam showed good activity against extended-spectrum beta-lactamase, non-carbapenem-resistant phenotype strains of Enterobacteriaceae (MIC50/90, 0.5/>32 µg/mL), it lacked useful activity against strains with a (MIC50/90, >32/>32 µg/mL; 1.6% S) carbapenem-resistant phenotype. Ceftolozane-tazobactam was the most potent (MIC50//90, 0.5/16 µg/mL) β-lactam agent tested against P. aeruginosa isolates, inhibiting 86.8% at an MIC of ≤4 µg/mL. P. aeruginosa exhibited high rates of resistance to cefepime (16.0%), ceftazidime (23.6%), meropenem (28.3%), and piperacillin-tazobactam (16.4%). Conclusions: Ceftolozane-tazobactam was the most active β-lactam agent tested against P. aeruginosa and demonstrated higher in vitro activity than available cephalosporins and piperacillin-tazobactam when tested against Enterobacteriaceae.
Descritores: Pseudomonas aeruginosa/efeitos dos fármacos
Cefalosporinas/farmacologia
Infecção Hospitalar/microbiologia
Ácido Penicilânico/análogos & derivados
Enterobacteriaceae/efeitos dos fármacos
Antibacterianos/farmacologia
-Fenótipo
Pseudomonas aeruginosa/isolamento & purificação
Ácido Penicilânico/farmacologia
Farmacorresistência Bacteriana Múltipla
Enterobacteriaceae/isolamento & purificação
Enterobacteriaceae/classificação
Monitoramento Epidemiológico
Tazobactam
América Latina
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-1039209
Autor: Barberino, Maria Goreth; Cruvinel, Silvia de Araujo; Faria, Célio; Salvino, Marco Aurélio; Silva, Marcio de Oliveira.
Título: Isolation of blaNDM-producing Enterobacteriaceae in a public hospital in Salvador, Bahia, Brazil
Fonte: Braz. j. infect. dis;22(1):47-50, Jan.-feb. 2018. tab, graf.
Idioma: en.
Resumo: ABSTRACT Carbapenemases have great importance in the global epidemiological scenario since infections with carbapenemase-producing bacteria are associated with high mortality, especially in hospitalized patients in intensive care units. This study describes two microorganisms producers of the New Delhi Metallo-b-lactamase, Klebsiella pneumoniae and Citrobacter freundii, from two patients admitted to a public hospital in Salvador, Bahia. These are the first clinical cases of New Delhi Metallo-b-lactamase described in microorganisms in the north and northeast Brazil. The isolates were characterized by antimicrobial susceptibility test, with resistance to all β-lactams including carbapenems, negative Modified Hodge Test and the synergy test with Ethylenediaminetetraacetic acid, Phenylboronic Acid and Cloxacillin was positive only with Ethylenediaminetetraacetic acid (difference of >5 mm in the inhibition zone between the disk without and with the inhibitor). Analysis by multiplex PCR for blaIMP, blaVIM, blaNDM, blaKPC and blaOXA-48 enzymes confirmed the presence of blaNDM gene. This report of two different New Delhi Metallo-b-lactamase-producing microorganisms in a different region of Brazil confirms the risk of spreading resistance genes between different species and emphasizes the need for prevention and control of infections caused by these pathogens, which have limited treatment options and have been linked to high mortality rates.
Descritores: Proteínas de Bactérias/metabolismo
beta-Lactamases/metabolismo
Enterobacteriaceae/enzimologia
Infecções por Enterobacteriaceae/microbiologia
-Proteínas de Bactérias/efeitos dos fármacos
beta-Lactamases/efeitos dos fármacos
Brasil
Carbapenêmicos/farmacologia
Evolução Fatal
Farmacorresistência Bacteriana
Enterobacteriaceae/isolamento & purificação
Enterobacteriaceae/efeitos dos fármacos
Reação em Cadeia da Polimerase Multiplex
Hospitais Públicos
Limites: Humanos
Masculino
Adulto
Idoso
Tipo de Publ: Relatos de Casos
Responsável: BR1.1 - BIREME


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Id: biblio-1011579
Autor: Pavez, Mónica; Troncoso, Claudia; Osses, Irma; Salazar, Rodrigo; Illesca, Vijna; Reydet, Patricia; Rodríguez, Claudio; Chahin, Carolina; Concha, Carla; Barrientos, Leticia.
Título: High prevalence of CTX-M-1 group in ESBL-producing enterobacteriaceae infection in intensive care units in southern Chile
Fonte: Braz. j. infect. dis;23(2):102-110, Mar.-Apr. 2019. tab, graf.
Idioma: en.
Projeto: Universidad de La Frontera; . CONICYT-PFCHA.
Resumo: ABSTRACT Enterobacteria-producing extended-spectrum β-lactamases (ESBL) play an important role in healthcare infections, increasing hospitalization time, morbidity and mortality rates. Among several ESBLs that emerge from these pathogens, CTX-M-type enzymes had the most successful global spread in different epidemiological settings. Latin America presents high prevalence of CTX-M-2 in ESBL-producing enterobacterial infections with local emergence of the CTX-M-1 group. However, this high prevalence of the CTX-M-1 group has not yet been reported in Chile. The aim of this study was to identify ESBLs among enterobacteria isolated from clinical samples of critically ill patients from southern Chile. One-hundred thirty seven ESBL-producing bacteria were isolated from outpatients from all critical patient units from Hernán Henríquez Aravena Hospital. Phenotype characterization was performed by antibiogram, screening of ESBL, and determination of minimum inhibitory concentration (MIC). PCR was used for genetic confirmation of resistance. Molecular typing was performed by ERIC-PCR. ESBL-producing isolates were identified as Klebsiella pneumoniae (n = 115), Escherichia coli (n = 18), Proteus mirabilis (n = 3), and Enterobacter cloacae (n = 1), presenting multidrug resistance profiles. PCR amplification showed that the strains were positive for blaSHV (n = 111/81%), blaCTX-M-1 (n = 116/84.7%), blaTEM (n = 100/73%), blaCTX-M-2 (n = 28/20.4%), blaCTX-M-9 (0.7%), blaPER-1 (0.7%), and blaGES-10 (0.7%). The multiple production of ESBL was observed in 93% of isolates, suggesting high genetic mobility independent of the clonal relationship. The high frequency of the CTX-M-1 group and a high rate of ESBL co-production are changing the epidemiology of the ESBL profile in Chilean intensive care units. This epidemiology is a constant and increasing challenge, not only in Chile, but worldwide.
Descritores: beta-Lactamases/genética
Enterobacteriaceae/enzimologia
Infecções por Enterobacteriaceae/enzimologia
Infecções por Enterobacteriaceae/epidemiologia
Unidades de Terapia Intensiva/estatística & dados numéricos
-Valores de Referência
beta-Lactamases/isolamento & purificação
DNA Bacteriano
Testes de Sensibilidade Microbiana
Chile/epidemiologia
Reação em Cadeia da Polimerase
Prevalência
Fatores de Risco
Enterobacteriaceae/isolamento & purificação
Enterobacteriaceae/efeitos dos fármacos
Infecções por Enterobacteriaceae/microbiologia
Técnicas de Genotipagem
Antibacterianos/farmacologia
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-1041821
Autor: Quirog, Cecilia; Nastro, Marcela; Di Conza, José.
Título: Escenario actual de la resistencia a la colistina mediada por plásmidos en América Latina / Current scenario of plasmid-mediated colistin resistance in Latin America
Fonte: Rev. argent. microbiol;51(1):93-100, mar. 2019. map, tab.
Idioma: en.
Resumo: Colistin resistance can occur by chromosomal mutations and by acquisition of plasmid-carrying determinants, mainly mcr-1. In the recent years, we have observed the out-burst of this resistance gene in our region. Due to the risk of the rapid dissemination of mcr-1, this finding has worried and alerted different actors from the health field and has become one of the most prolific topics. Our review compiles available reports of well-documented mcr-1-positive strains of Enterobacteriaceae, obtained from different samples in Argentina and other countries of Latin America. Furthermore, it addresses the association of mcr-1 with ESBL resistance markers and outlines the platforms involved in their dissemination.

La resistencia a la colistina puede ocurrir por mutaciones cromosómicas o por la adquisición de determinantes localizados en plásmidos, el principal es mcr-1. En los últimos años hemos observado la explosiva aparición de este gen de resistencia en nuestra región. Debido al riesgo que implica la rápida diseminación de mcr-1, este hallazgo ha preocupado y alertado a los diferentes actores del área de la salud, y se ha convertido en uno de los temas de investigación más importantes. La presente revisión compila los informes de aislamientos portadores de mcr-1 debidamente documentados en Enterobacteriaceae, obtenidos de diferentes muestras en Argentina y otros países de América Latina. Además, aborda la asociación de mcr-1 con otros marcadores de resistencia, como las BLEE, y describe las plataformas involucradas en su diseminación.
Descritores: Plasmídeos/agonistas
Colistina/antagonistas & inibidores
-Associação
Fatores R/análise
Biomarcadores
Enterobacteriaceae/isolamento & purificação
Tipo de Publ: Ensaio Clínico
Estudo de Avaliação
Responsável: AR635.1 - FCVyS - Servicio de Información y Documentación


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Id: lil-745216
Autor: Colquechagua Aliaga, Fabiola; Sevilla Andrade, Carlos; Gonzales Escalante, Edgar.
Título: Enterobacterias productoras de betalactamasas de espectro extendido en muestras fecales en el Instituto Nacional de Salud del Niño, Perú / Extended-spectrum beta-lactamase (ESBL)-producing enterobacteriaceae in fecal samples at the National Institute of Child Health, Peru
Fonte: Rev. peru. med. exp. salud publica;32(1):26-32, ene.-mar. 2015. tab.
Idioma: es.
Resumo: Objetivos. Describir la frecuencia de enterobacterias productoras de betalactamasas de espectro extendido (BLEE) en muestras fecales en el Instituto Nacional de Salud del Niño, Lima, Perú. Materiales y métodos. Se analizaron muestras de heces recibidas entre julio de 2012 y marzo de 2013, se trabajó con colonias sospechosas de ser enterobacterias productoras de BLEE que se desarrollaron en el agar Karmali; se realizó la identificación bioquímica por métodos convencionales y la confirmación del fenotipo BLEE. El análisis genotípico para detectar el gen de betalactamasa de la familia CTX-M se realizó por PCR. Resultados. De 235 muestras fecales se aisló un 64,2% de enterobacterias productoras de BLEE siendo Escherichia coli 86,1%, Klebsiella pneumoniae 7,9%, Salmonella sp. 2,6%, Enterobacter cloacae 2,0% y Proteus mirabilis 1,3%. El 89,1% de las enterobacterias productoras de BLEE presentaron el gen blaCTX-M. Se encontró una alta resistencia al ácido nalidíxico 84,8%, ciprofloxacina 74,2% y trimetoprimsulfametoxazol 81,5%. La resistencia a la amikacina fue de 1,3% y todos los aislados fueron sensibles al imipenem y meropenem. Conclusiones. Se encontró una alta frecuencia de enterobacterias productoras de BLEE en muestras fecales de pacientes ambulatorios atendidos en los consultorios externos y emergencia del Instituto Nacional de Salud del Niño en Perú.

Objectives. To describe the frequency of extended-spectrum beta-lactamase (ESBL)-producing enterobacteriaceae in fecal samples at the National Institute of Child Health, Lima, Peru. Materials and methods. Stool samples received between July 2012 and March 2013 with colonies suspected to be ESBL-producing enterobacteriaceae that developed in Karmali agar were analyzed. Conventional methods were performed for biochemical identification and the confirmation of the ESBL phenotype. Genotypic analysis to detect the beta-lactamase gene CTX-M family was performed by PCR. Results. Of the 235 fecal samples analyzed, 64.2% of ESBL-producing enterobacteria was isolated being 86.1% Escherichia coli, 7.9% Klebsiella pneumoniae, 2.6% Salmonella sp, 2.0% Enterobacter cloacae, and 1.3% Proteus mirabilis. 89.1% of the ESBL-producing enterobacteria presented the CTX-M gene. We found high resistance to nalidixic acid 84.8%, 74.2% ciprofloxacin and trimethoprim-sulfamethoxazole 81.5%.The resistance to amikacin was 1.3% and all isolates were susceptible to imipenem and meropenem. Conclusions. A high frequency of ESBL-producing enterobacteria was found in fecal samples of outpatients seen in the outpatient and emergency departments of the National Institute of Child Health of Peru.
Descritores: Enterobacteriaceae
Fezes
Penicilinase
-Epidemiologia Descritiva
Estudos Observacionais como Assunto
Estudos Transversais
Peru
Limites: Humanos
Responsável: PE14.1 - Biblioteca de la Sede Central



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BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde