Base de dados : LILACS
Pesquisa : B03.440.450.900.604 [Categoria DeCS]
Referências encontradas : 4 [refinar]
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Texto completo SciELO Chile
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Id: lil-524888
Autor: Shen, Kaili; Shen, Chaofeng; Lu, Yuan; Tang, Xianjin; Zhang, Congkai; Chen, Xincai; Shi, Jiyan; Lin, Qi; Chen, Yingxu.
Título: Hormesis response of marine and freshwater luminescent bacteria to metal exposure
Fonte: Biol. Res;42(2):183-187, 2009. graf.
Idioma: en.
Projeto: Program for Changjiang Scholars; . Innovative Research University Teams.
Resumo: The stimulatory effect of low concentrations of toxic chemicals on organismal metabolism, referred to as hormesis, has been found to be common in the widely used luminescence bioassay. This paper aims to study the hormesis phenomenon in both marine and freshwater luminescent bacteria, named Photobacterium phosphorem and Vibrio qinghaiensis. The effects of Cu (II), Zn (II), Cd (II) and Cr (VI) on luminescence of these two bacteria were studied for 0 to 75 minutes exposure by establishing dose- and time-response curves. A clear hormesis phenomenon was observed in all four testing metals at low concentrations under the condition of luminescence assays.
Descritores: Metais Pesados/farmacologia
Photobacterium/efeitos dos fármacos
Vibrio/efeitos dos fármacos
-Cádmio/farmacologia
Cromo/farmacologia
Cobre/farmacologia
Água Doce
Luminescência
Água do Mar
Fatores de Tempo
Zinco/farmacologia
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Texto completo SciELO Chile
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Id: lil-456611
Autor: Mancuso, Monique; Avendaño-Herrera, Rubén; Zaccone, R; Toranzo, Alicia e; Magariños, Beatriz.
Título: Evaluation of different DNA-based fingerprinting methods for typing Photobacterium damselae ssp. piscicida
Fonte: Biol. Res;40(1):85-92, 2007. ilus, tab.
Idioma: en.
Projeto: Spain. Ministerio de Ciencia y Tecnología.
Resumo: This study evaluates the effectiveness of three different molecular techniques, repetitive extragenic palindromic PCR (REP-PCR), enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) and the random amplified polymorphic DNA (RAPD-PCR) for rapid typing of Photobacterium damselae ssp. piscicida strains isolated from different species of marine fish and geographic areas. The results obtained by the three methods showed that RAPD and ERIC-PCR were more discriminative for suitable rapid typing of Ph. damselae ssp. piscicida than REP-PCR. The analysis of DNA banding patterns generated by both molecular methods (RAPD and ERIC-PCR) clearly separated the strains into two main groups that strongly correlated with their geographic origin. Moreover, the REP-PCR analysis was less reproducible than the RAPD and ERIC-PCR methods and does not allow the establishment of genetic groups. RAPD and ERIC-PCR constitute valuable tools for molecular typing of Ph. damselae ssp. piscicida strains, which can be used in epidemiological studies of photobacteriosis infections.
Descritores: Técnicas de Tipagem Bacteriana/métodos
Impressões Digitais de DNA/métodos
Variação Genética
Photobacterium/classificação
-DNA Bacteriano/genética
Peixes/microbiologia
Photobacterium/genética
Reação em Cadeia da Polimerase/métodos
Técnica de Amplificação ao Acaso de DNA Polimórfico
Sequências Repetitivas de Ácido Nucleico/genética
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Texto completo SciELO Brasil
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Id: lil-355156
Autor: Nunes-Halldorson, Vânia da Silva; Duran, Norma Letícia.
Título: Bioluminescent bacteria: lux genes as environmental biosensors
Fonte: Braz. j. microbiol;34(2):91-96, Apr.-Jun. 2003.
Idioma: en.
Resumo: Bioluminescent bacteria are widespread in natural environments. Over the years, many researchers have been studying the physiology, biochemistry and genetic control of bacterial bioluminescence. These discoveries have revolutionized the area of Environmental Microbiology through the use of luminescent genes as biosensors for environmental studies. This paper will review the chronology of scientific discoveries on bacterial bioluminescence and the current applications of bioluminescence in environmental studies, with special emphasis on the Microtox toxicity bioassay. Also, the general ecological significance of bioluminescence will be addressed.
Descritores: Bactérias/genética
Bactérias/química
Medições Luminescentes
Testes de Toxicidade/métodos
-Técnicas Biossensoriais
Microbiologia Ambiental
Luciferases
Photobacterium/genética
Photobacterium/química
Vibrio/genética
Vibrio/química
Tipo de Publ: Revisão
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica


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Id: lil-99491
Autor: Schall, V. T; Vasconcellos, M. C; Valent, G. U; Sato, M. I. Z; Furlan, E. V; Sanchez, P. S.
Título: Evaluation of the genotoxic activity and acute toxicity of Euphorbia splendens latex, a molluscicide for the control of Schistosomiasis
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;24(6):573-82, 1991. tab.
Idioma: en.
Resumo: The latex of euphorbia splendens var. hislopii has a molluscicidal action at low concentration (LD90 less than 1.5 ppm or 1.5 */ml) against the vector snails of schistosomiasis. In the present study, the latex in natura or after lyophilization was submitted to the Ames test and the chromotest to evaluate genotoxicity, to the Microtox System to determine acute toxicity, and to the Chinese hamster ovary cell assay (CHO) to measure cytotoxicity. The latex had no mutagenic activity in the presence or absence of S9 toward the TA98 and TA100 strains of Salmonella typhimurium (Ames test) at concentration up to 200 */plate (in natura) and of 200 *g/plate (lyophilized). The lyophilized latex had no genotoxic activity (Chromotest) and acute toxic effect on Photobacterium phosphoreum at concentrations up to 445 *g/ml, whereas the sample in natura had a toxic effect with an EC50 of 148,000 *l/l (or ppm). In the CHO/cytotoxicity assay, the lyophilized latex had no cytotoxicit effect in quantities up to 200 *g. The latex was found to have no acute toxicity or mutagenic at the concentrations of 10 to 12 *g/ml (or ppm) that are being proposed for molluscicidal use in the field
Descritores: Látex/toxicidade
Moluscocidas/toxicidade
Extratos Vegetais/toxicidade
-Células CHO/efeitos dos fármacos
Avaliação Pré-Clínica de Medicamentos
Escherichia coli/efeitos dos fármacos
Látex/metabolismo
Photobacterium/efeitos dos fármacos
Extratos Vegetais/metabolismo
Salmonella typhimurium/efeitos dos fármacos
Esquistossomose/prevenção & controle
Limites: Animais
Cricetinae
Responsável: BR26.1 - Biblioteca Central



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