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Texto completo SciELO Chile
Texto completo
Id: lil-640497
Autor: Panwichian, Saijai; Kantachote, Duangporn; Wittayaweerasak, Banjong; Mallavarapu, Megharaj.
Título: Removal of heavy metals by exopolymeric substances produced by resistant purple nonsulfur bacteria isolated from contaminated shrimp ponds
Fonte: Electron. j. biotechnol;14(4):2-2, July 2011. ilus, tab.
Idioma: en.
Projeto: Price of Songkla University.
Resumo: Two purple nonsulfur bacteria (PNSB) strains, Rhodobium marinum NW16 and Rhodobacter sphaeroides KMS24 were investigated for their potential to remove heavy metals (HMs) from contaminated shrimp pond water. Tolerance of both PNSB strains growing with both microaerobic-light and aerobic-dark conditions, based on their minimum inhibitory concentrations, was in the order of Cu2+ > Zn2+ > Cd2+ (Pb precipitation occurred at 0.34 mM). Results from a scanning electron microscope equipped with an energy dispersive X-ray spectrometer (SEM-EDX) indicated that Cu2+ and Zn2+ altered the cellular morphology of both strains and accumulated HMs were found in their cells. The highest amounts of both cations were found in their cell walls followed by the cytoplasm and cell membrane. Using the highest concentrations (mM) of HMs found in shrimp pond of 0.0067 Cd2+, 0.54 Cu2+, 0.30 Pb2+, 0.89 Zn2+ and 3 percent NaCl under both incubating conditions exopolymeric substances (EPS) produced by both strains showed a greater removal of all HMs (average percentages; 90.52-97.29) than their cells (average percentages; 14.02-75.03).
Descritores: Aquicultura
Poluição Ambiental
Metais Pesados/metabolismo
-Biodegradação Ambiental
Testes de Sensibilidade Microbiana
Rhodobacter sphaeroides
Responsável: CL1.1 - Biblioteca Central

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Texto completo SciELO Chile
Texto completo
Id: lil-531890
Autor: Sangkharak, Kanokphorn; Prasertsan, Poonsuk.
Título: Nutrient optimization for production of polyhydroxybutyrate from halotolerant photosynthetic bacteria cultivated under aerobic-dark condition
Fonte: Electron. j. biotechnol;11(3):83-94, July 2008. ilus, graf, tab.
Idioma: en.
Resumo: Three halotolerant bacterial strains; Rhodobacter sphaeroides ES16 (the wild type) and the two mutant strains of R. sphaeroides ES16, namely N20 and U7, were cultivated in glutamate-malate (GM) medium and screened for production of polyhydroxybutyrate (PHB). The mutant strains N20 and U7 were found to accumulate PHB (53.9 and 42.0 percent of DCW, respectively) 3.6 and 2.8 times higher than the wild type strain (19.5 percent of DCW), respectively. R. sphaeroides N20 were selected for studies on the effects of nutrient and environmental conditions on PHB accumulation. The optimal condition was 4 g/l acetate, 0.02 g/l (NH4)2SO4, C/N ratio of 6:1, 1.0 g/l K2HPO4, 1.0 g/l KH2PO4 and 3 percent NaCl with initial pH at 7.0. Under this optimal condition, the maximum PHB accumulation increased from 53.9 percent to 88 percent of DCW and 9.11 ± 0.08 g/l biomass, 8.02 +/- 0.10 g/l PHB concentration were achieved after 60 hrs cultivation at 37ºC. These results are the highest values ever obtained from photosynthetic bacteria reported so far.
Descritores: Bactérias Gram-Negativas Fotossintetizantes Oxigênicas
Rhodobacter sphaeroides/enzimologia
Rhodobacter sphaeroides/metabolismo
-Cromatografia Gasosa
Microscopia Eletrônica de Transmissão
Nitrogênio/administração & dosagem
Responsável: CL1.1 - Biblioteca Central

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Texto completo SciELO Brasil
Texto completo
Id: lil-405531
Autor: Braun, P; Gebhardt, R; Kwa, L; Doster, W.
Título: High pressure near infrared study of the mutated light-harvesting complex LH2
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;38(8):1273-1278, Aug. 2005. ilus, graf.
Idioma: en.
Conferência: Apresentado em: International Conference on High Pressure Bioscience and Biotechnology, 3, Rio de Janeiro, Sept. 26-30, 2004.
Resumo: The pressure sensitivities of the near infrared spectra of the light-harvesting (LH2) complex and a mutant complex with a simplified BChl-B850 binding pocket were compared. In the mutant an abrupt change in the spectral properties occurred at 250 MPa, which was not observed with the native sample. Increased disorder due to collapse of the chromophore pocket is suggested.
Descritores: Escherichia coli/genética
Pressão Hidrostática
Complexos de Proteínas Captadores de Luz/genética
Rhodobacter sphaeroides/genética
Espectroscopia de Luz Próxima ao Infravermelho
-Complexos de Proteínas Captadores de Luz/química
Modelos Moleculares
Responsável: BR1.1 - BIREME

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