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Id: biblio-1021543
Autor: Panyachanakul, Titiporn; Kitpreechavanich, Vichien; Tokuyama, Shinji; Krajangsang, Sukhumaporn.
Título: Poly(DL-lactide)-degrading enzyme production by immobilized Actinomadura keratinilytica strain T16-1 in a 5-L fermenter under various fermentation processes
Fonte: Electron. j. biotechnol;30:71-76, nov. 2017. graf, ilus, tab.
Idioma: en.
Projeto: National Research Council of Thailand.
Resumo: Background: Poly(DL-lactic acid), or PDLLA, is a biodegradable polymer that can be hydrolyzed by various types of enzymes. The protease produced by Actinomadura keratinilytica strain T16-1 was previously reported to have PDLLA depolymerase activity. However, few studies have reported on PDLLA-degrading enzyme production by bacteria. Therefore, the aims of this study were to determine a suitable immobilization material for PDLLA-degrading enzyme production and optimize PDLLA-degrading enzyme production by using immobilized A. keratinilytica strain T16-1 under various fermentation process conditions in a stirrer fermenter. Results: Among the tested immobilization materials, a scrub pad was the best immobilizer, giving an enzyme activity of 30.03 U/mL in a shake-flask scale. The maximum enzyme activity was obtained at aeration 0.25 vvm, agitation 170 rpm, 45°C, and 48 h of cultivation time. Under these conditions, a PDLLA-degrading enzyme production of 766.33 U/mL with 15.97 U/mL·h productivity was observed using batch fermentation in a 5-L stirrer fermenter. Increased enzyme activity and productivity were observed in repeated-batch (942.67 U/mL and 19.64 U/mL·h) and continuous fermentation (796.43 U/mL and 16.58 U/mL·h) at a dilution rate of 0.013/h. Scaled-up production of the enzyme in a 10-L stirrer bioreactor using the optimized conditions showed a maximum enzyme activity of 578.67 U/mL and a productivity of 12.06 U/mL·h. Conclusions: This research successfully scaled-up the enzyme production to 5 and 10 L in a stirrer fermenter and is helpful for many applications of poly(lactic acid).
Descritores: Poliésteres/metabolismo
Actinomycetales/enzimologia
Enzimas/biossíntese
-Biodegradação Ambiental
Reatores Biológicos
Enzimas/metabolismo
Enzimas Imobilizadas
Fermentação
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Brasil
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Id: biblio-889136
Autor: Yang, Shanjun; Yu, Mingjia; Chen, Jianming.
Título: Draft genome analysis of Dietzia sp. 111N12-1, isolated from the South China Sea with bioremediation activity
Fonte: Braz. j. microbiol;48(3):393-394, July-Sept. 2017.
Idioma: en.
Projeto: Scientific Research Foundation of Third Institute of Oceanography; . National Science Foundation of China.
Resumo: Abstract Dietzia sp. 111N12-1, isolated from the seawater of South China Sea, shows strong petroleum hydrocarbons degradation activity. Here, we report the draft sequence of approximately 3.7-Mbp genome of this strain. To the best of our knowledge, this is the first genome sequence of Dietzia strain isolated from the sea. The genome sequence may provide fundamental molecular information on elucidating the metabolic pathway of hydrocarbons degradation in this strain.
Descritores: Água do Mar/microbiologia
Actinomycetales/isolamento & purificação
Actinomycetales/genética
Genoma Bacteriano
Hidrocarbonetos/metabolismo
-Filogenia
Biodegradação Ambiental
Actinomycetales/classificação
Actinomycetales/metabolismo
Petróleo/metabolismo
Sequência de Bases
China
Responsável: BR1.1 - BIREME


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Id: lil-230791
Autor: Lacaz, Carlos da Silva; Porto, Edward; Heins-Vaccari, Elisabeth; Melo, Natalina Takahashi de.
Título: Guia de identificaçäo fungos, actinomicetos, algas de interesse médico / Guide of identification: fungi, actinomycetales, algas of physician interest.
Fonte: Säo Paulo; Sarvier; 1998. 445 p. ilus, tab.
Idioma: pt.
Descritores: Actinomycetales/classificação
Eucariotos/classificação
Fungos/classificação
-Terminologia
Tipo de Publ: Guia
Responsável: BR1.1 - BIREME
BR1.1/3281.00; BR67.1; 616.969 32


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Texto completo SciELO Chile
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Id: biblio-840314
Autor: Lin, Ling; Liu, Xiaozhou; Zhou, Yating; Guan, Linyan; Jiajia, He; Huang, Weiqian.
Título: A novel pH-stable, endoglucanase (JqCel5A) isolated from a salt-lake microorganism, Jonesia quinghaiensis
Fonte: Electron. j. biotechnol;19(6):56-62, Nov. 2016. ilus.
Idioma: en.
Projeto: Key Laboratory of Biotic Environment and Ecology Safety in Anhui Province. Anhui Provincial Natural Science Foundation.
Resumo: Background: Endoglucanase, one of three type cellulases, can randomly cleave internal p-1,4-linkages in cellulose polymers. Thus, it could be applied in agricultural and industrial processes. Results: A novel endoglucanase gene (JqCel5A) was cloned from Jonesia quinghaiensis and functionally expressed in Escherichia coli Rosetta (DE3). It contained 1722 bp and encoded a 573-residue polypeptide consisting of a catalytic domain of glycoside hydrolase family 5 (GH5) and a type 2 carbohydrate-binding module (CBM2), together with a predicted molecular mass of 61.79 kD. The purified JqCel5A displayed maximum activity at 55°C and pH 7.0, with 21.7 U/mg, 26.19 U/mg and 4.81 U/mg towards the substrate carboxymethyl cellulose, barley glucan and filter paper, respectively. Interestingly, JqCel5A exhibited high pH stability over a broad pH range of pH (3-11), and had good tolerance to a wide variety of deleterious chemicals including heavy metals and detergent. The catalytic mechanism of JqCel5A was also investigated by site mutagenesis and homology-modeling in this study. Conclusions: It was believed that these properties might make JqCel5A to be potentially used in the suitable industrial catalytic condition, which has a broad pH fluctuation and/or chemical disturbance.
Descritores: Actinomycetales/enzimologia
Celulases/química
Celulases/isolamento & purificação
-Celulases/genética
Concentração de Íons de Hidrogênio
Testes de Mutagenicidade
Temperatura Ambiente
Responsável: CL1.1 - Biblioteca Central


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Id: lil-714926
Autor: Dickson, Cinthia; Elías-Costa, María Rosa I. de.
Título: Dermatofilosis humana y animal. Presentación de un caso atípico y revisión de la literatura / Human and animal dermatophilosis. An unusual case report and review of the literature
Fonte: Dermatol. argent;16(5):349-353, sep.-oct. 2010. ilus.
Idioma: es.
Resumo: La dermatofilosis es una infección causada por una bacteria Gram positiva perteneciente al orden de los Actinomycetales, llamada Dermatophilus congolensis. Se presenta como una dermatosis de carácter agudo, subagudo o crónico que afecta preferentemente al ganado pero también a una amplia variedad de animales domésticos y salvajes y al hombre. Tiene distribución universal, pero con mayor prevalencia en climas húmedos, tropicales y subtropicales. Es considerada una zoonosis menor que puede causar grandes perjuicios económicos en la industria del ganado y el cuero. Se realiza una revisión del tema junto con la presentación de un caso clínico sumamente atípico.

Dermatophilosis is an acute, subacute or chronic skin disease affecting a wide range of species of animals and man. It is caused by a Gram (+) bacteria of the order of the Actinomycetales named Dermatophilus congolensis. Presenting as an acute, subacute or chronic dermatosis affecting primarily cattle, but a wide variety of domestic and wild animals, and humans, as well. It is distributed worldwide but more prevalent in the humid tropical and subtropic areas. It is essential to emphasize the importance of this disease in livestock industry and leather production. The disease is reviewed and an unusual case is reported.
Descritores: Actinomycetales
Pele/patologia
-Dermatopatias Bacterianas/diagnóstico
Dermatopatias Bacterianas/epidemiologia
Gentamicinas/uso terapêutico
Infecções por Actinomycetales/microbiologia
Pomadas/uso terapêutico
Limites: Seres Humanos
Feminino
Meia-Idade
Tipo de Publ: Relatos de Casos
Responsável: AR144.1 - CIBCHACO - Centro de Información Biomedica del Chaco


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Id: lil-688595
Autor: Procópio, Luciano; Silva, Michele de Cassia Pereira e; Elsas, Jan Dirk van; Seldin, Lucy.
Título: Transcriptional profiling of genes involved in n-hexadecane compounds assimilation in the hydrocarbon degrading Dietzia cinnamea P4 strain
Fonte: Braz. j. microbiol;44(2):639-647, 2013. ilus, graf, tab.
Idioma: en.
Resumo: The petroleum-derived degrading Dietzia cinnamea strain P4 recently had its genome sequenced and annotated. This allowed employing the data on genes that are involved in the degradation of n-alkanes. To examine the physiological behavior of strain P4 in the presence of n-alkanes, the strain was grown under varying conditions of pH and temperature. D. cinnamea P4 was able to grow at pH 7.0-9.0 and at temperatures ranging from 35 ºC to 45 ºC. Experiments of gene expression by real-time quantitative RT-PCR throughout the complete growth cycle clearly indicated the induction of the regulatory gene alkU (TetR family) during early growth. During the logarithmic phase, a large increase in transcriptional levels of a lipid transporter gene was noted. Also, the expression of a gene that encodes the protein fused rubredoxin-alkane monooxygenase was enhanced. Both genes are probably under the influence of the AlkU regulator.
Descritores: Actinomycetales/genética
Actinomycetales/metabolismo
Alcanos/metabolismo
Perfilação da Expressão Gênica
Genes Bacterianos
Hidrocarbonetos/metabolismo
Redes e Vias Metabólicas/genética
-Actinomycetales/crescimento & desenvolvimento
Biotransformação
Concentração de Íons de Hidrogênio
Reação em Cadeia da Polimerase em Tempo Real
Temperatura Ambiente
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-634513
Autor: Negroni, R.; López Daneri, G.; Arechavala, A.; Bianchi, M. H.; Robles, A. M..
Título: Estudio clínico y microbiológico de los micetomas observados en el Hospital de Infecciosas Francisco J. Muñiz en el período 1989-2004 / Clinical and microbiological study of mycetomas at the Muñiz Hospital of Buenos Aires between 1989 and 2004
Fonte: Rev. argent. microbiol;38(1):13-18, ene.-mar. 2006. ilus, tab.
Idioma: es.
Resumo: Se presentan las características clínicas, microbiológicas y los resultados del tratamiento de 76 casos de micetomas observados en el período 1989-2004 en el Hospital Muñiz. Cuarenta y nueve fueron varones y 27 mujeres, con una edad promedio de 43,4 años. La mayor parte de los pacientes adquirió la infección en nuestro país, las provincias más afectadas fueron Santiago del Estero con 31 casos y el Chaco con 11; 8 enfermos procedían del exterior, 6 de Bolivia y 2 de Paraguay. El promedio de evolución de la enfermedad fue de 9,2 años. Las localizaciones más comunes fueron las de los miembros inferiores: pies 63, tobillos 3 y rodillas 2. Se comprobó compromiso óseo en 48 casos y adenomegalias en 5. Fueron identificados los siguientes agentes causales: Madurella grisea 29 casos, Actinomadura madurae 26, Scedosporium apiospermum 5, Nocardia brasiliensis 5, Acremoniun spp. 4 (Acremonium falciforme 2, Acremonium kiliense 1 y Acremonium recifei 1), Madurella mycetomatis 3, Fusarium solani 2, Nocardia asteroides y Streptomyces somaliensis 1 caso cada uno. Los tratamientos más frecuentemente utilizados fueron ketoconazol o itraconazol en los micetomas maduromicósicos y la asociación de cotrimoxazol con ciprofloxacina o amicacina en los micetomas actinomicéticos. La amputación del miembro afectado se realizó en 6 casos, 25 pacientes alcanzaron la remisión clínica completa y 34 presentaron mejorías importantes.

This work presents clinical, microbiological and outcome data collected from 76 patients with mycetomas at the Muñiz Hospital from 1989 to 2004. Forty-nine patients were male and 27 female; the mean age was 43.4 years. The majority of the patients acquired the infection in Argentina: the most affected provinces were Santiago del Estero with 31 cases, and Chaco with 11; 8 cases came from other countries (Bolivia 6 and Paraguay 2). The mean evolution of the disease was 9.2 years. The most frequently observed sites were: feet 63 cases, ankles 3, and knees 2. Forty-eight patients had bone lesions and 5, adenomegalies. The following etiological agents were identified: Madurella grisea 29 cases, Actinomadura madurae 26, Scedosporium apiospermum 5, Nocardia brasiliensis 5, Acremonium spp. 4 (Acremonium falciforme 2, Acremonium kiliense 1, Acremonium recifei 1), Madurella mycetomatis 3, Fusarium solani 2, Nocardia asteroides 1 and Streptomyces somaliensis 1. The main drugs used in the treatments were ketoconazole and itraconazole for maduromycotic mycetomas, and cotrimoxazole associated with ciprofloxacin or amikacin for actinomycetic mycetoma. Six patients had to undergo amputation, 25 cases achieved complete clinical remission and 34 showed remarkable improvement.
Descritores: Infecções por Actinomycetales/epidemiologia
Micetoma/epidemiologia
-Amputação
Infecções por Actinomycetales/tratamento farmacológico
Infecções por Actinomycetales/microbiologia
Infecções por Actinomycetales/cirurgia
Actinomycetales/isolamento & purificação
Doenças dos Trabalhadores Agrícolas/tratamento farmacológico
Doenças dos Trabalhadores Agrícolas/epidemiologia
Doenças dos Trabalhadores Agrícolas/microbiologia
Doenças dos Trabalhadores Agrícolas/cirurgia
Antibacterianos/uso terapêutico
Antifúngicos/uso terapêutico
Argentina/epidemiologia
Terapia Combinada
Fusarium
Dermatoses do Pé/tratamento farmacológico
Dermatoses do Pé/epidemiologia
Dermatoses do Pé/microbiologia
Dermatoses do Pé/cirurgia
Madurella/efeitos dos fármacos
Madurella/isolamento & purificação
Fungos Mitospóricos/isolamento & purificação
Micetoma/tratamento farmacológico
Micetoma/microbiologia
Micetoma/cirurgia
Nocardiose/tratamento farmacológico
Nocardiose/epidemiologia
Nocardiose/microbiologia
Nocardiose/cirurgia
Osteíte/tratamento farmacológico
Osteíte/etiologia
Osteíte/microbiologia
Osteíte/cirurgia
Indução de Remissão
Estudos Retrospectivos
Resultado do Tratamento
Limites: Adolescente
Adulto
Idoso
Feminino
Seres Humanos
Masculino
Meia-Idade
Responsável: AR1.2 - Instituto de Investigaciónes Epidemiológicas


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Texto completo SciELO Chile
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Id: lil-508965
Autor: Filippi N, Jorge; Quezada S, Felipe; Lagos L, Marcela; García C, Patricia.
Título: Micetoma por Actinomadura madurae en el pie: reporte de un caso en Chile / Actinomadura madurae mycetoma of the foot: report of one case
Fonte: Rev. méd. Chile;136(11):1448-1452, nov. 2008. ilus.
Idioma: es.
Resumo: Mycetoma is a chronic infection that affects skin, subcutaneous tissue and bone. Its etiology can be mycotic or bacterial. It affects mainly the lower extremities ofmiddie age men livingin tropical climates. We repon a 44 year-old male ¡ivingin a template zone, consulting for swelling and pain in the left foot, lasting for 10 years. Physical examination showed a swollen left foot with hyperpigmented skin and a few crustedpapules. Radiology showed an extensive bone involvement of the midfoot with several oval and radiolucid images. Magnetic resonance showed son and bone tissue involvement, with múltiple oval and low intensity images in TI and T2. The biopsy was compatible with an unspecific chronic osteomyelitis. A bacterial identification by polymerase chain reaction and sequencing in the biopsy determined the presence of an Actinomadura madurae. Treatment with cotrimoxazol was started).
Descritores: Actinomycetales/genética
Dermatoses do Pé/microbiologia
Micetoma/microbiologia
-Actinomycetales/classificação
Actinomycetales/isolamento & purificação
Anti-Infecciosos/uso terapêutico
Dermatoses do Pé/diagnóstico
Dermatoses do Pé/tratamento farmacológico
Micetoma/diagnóstico
Micetoma/tratamento farmacológico
Reação em Cadeia da Polimerase
Combinação Trimetoprima e Sulfametoxazol/uso terapêutico
Limites: Adulto
Seres Humanos
Masculino
Tipo de Publ: Relatos de Casos
Responsável: BR1.1 - BIREME


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Texto completo SciELO Brasil
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Id: lil-489051
Autor: Koller, Felipe L; Borowski, Sandra M; Asanome, William; Hein, Gisele; Lagemann, Franco L; Driemeier, David; Barcellos, David Emilio S. N. de.
Título: Abscessos dentários periapicais em leitões com síndrome multissistêmica do definhamento / Dental periapical abscesses in piglets affected by postweaning multisystemic wasting syndrome PMWS
Fonte: Pesqui. vet. bras = Braz. j. vet. res;28(6):271-274, jun. 2008. ilus, tab.
Idioma: pt.
Resumo: Duzentos e oitenta leitões, entre 40 e 70 dias de idade, afetados pela sindrome multissistêmica do definhamento (SMD) foram examinados após eutanásia para pesquisa de lesões dentárias. Pelo menos um abscesso periapical foi observado em 58 leitões (20,7 por cento), dos quais 12 apresentaram abscessos múltiplos. Terceiros incisivos superiores, 3os incisivos inferiores, caninos superiores, caninos inferiores e outros dentes apresentaram respectivamente 22 (31,4 por cento), 16 (22,9 por cento), 4 (5,7 por cento), 23 (32,9 por cento) e 5 (7,1 por cento) abscessos periapicais. A maior prevalência de abscessos observada nos dentes 3os incisivos e caninos inferiores está provavelmente associada com a maior área de corte ou desgaste que sofrem esses dentes. Entre as bactérias isoladas de amostras de 65 abscessos, Streptococcus sp. foi a mais prevalente e esteve presente em 21,48 por cento e 27,7 por cento dos isolados em aerobiose e anaerobiose, respectivamente. Na segunda posição em prevalência estiveram as bactérias corineformes, as quais foram mais freqüentemente isoladas em atmosfera anaeróbica do que aeróbica. Houve preponderância de isolamentos de bactérias Gram-positivas. Não foi possível determinar se as co-infecções bacterianas predispuseram às lesões características de SMD ou foram conseqüentes à imunossupressão causada pela infecção com PCV2.

Swine producers have historically resected needle teeth of newborn pigs to prevent potential injuries to the sow mammary gland and faces of littermates. However, the possible impact of this practice on pig performance has been questioned. As part of a study, 280 PMWS affected piglets, with 40-70 days of age, were examined for the presence of dental lesions immediately after euthanasia. Most pigs were confirmed as PMWS by the detection of typical microscopic lesions and immunohistochemical pattern. At least one periapical abscess was observed in 58 piglets (20.7 percent), of which 12 had multiple abscesses. There were 22 (31.4 percent), 16 (22.9 percent), 4 (5.7 percent), 23 (32.9 percent), and 5 (7.1 percent) abscesses in 3rd upper incisives, 3rd lower incisives, upper canines, lower canines, and other teeth, respectively. The higher prevalence of dental abscesses observed in 3rd incisives and lower canines is probably associated with the wider area of grinding or clipping to which these teeth were subjected. Among bacteria isolated from samples collected from 65 abscesses, Streptococcus sp. was the most prevalent bacteria and was present in 21.48 percent and 27.7 percent of the aerobic and anaerobic isolates, respectively. In the second most frequent group were coryneform microorganisms, which showed higher rate of isolation under anaerobic atmosphere than in aerobic culture. There was preponderance of Gram-positive isolates. It could not be determined whether these bacterial co-infections predisposed pigs to development of PMWS-type lesions or if were subsequent to the PCV2 infection.
Descritores: Abscesso Periapical/etiologia
Abscesso Periapical/patologia
Abscesso Periapical/veterinária
Actinomycetales/isolamento & purificação
Síndrome Definhante Multissistêmico de Suínos Desmamados/diagnóstico
Streptococcus/isolamento & purificação
Suínos/microbiologia
Limites: Animais
Recém-Nascido
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Texto completo SciELO Venezuela
Texto completo
Id: lil-357485
Autor: Pardi, Germán; Perrone, Marianella; Acevedo, Ana María; Mazzali de Ilja, Rosandra.
Título: Estudios sobre Rothia dentocariosa en pacientes con caries dental / Studies on Rothia dentocariosa in patients with dental caries
Fonte: Acta odontol. venez;41(3):20-30, dic. 2003. tab, graf.
Idioma: es.
Resumo: Tomando en consideración que la caries dental es una enfermedad producida por muchos de los microorganismos que conforman la placa dental, entre ellos Rothia dentocariosa, se decidió identificar a esta bacteria en pacientes que presentaban dicha entidad. En este estudio se seleccionaron 65 pacientes de la Facultad de Odontología de la UCV, los cuales fueron divididos en 2 grupos: del primer grupo (32 pacientes con caries dental), se tomaron muestras de las lesiones cariosas; se tomó además una muestra de placa a una paciente que jamás había tenido caries dental. Todas las muestras fueron transportadas al laboratorio en el medio de caldo tripticasa de soya para allí ser sembradas en tripticasa de soya en condiciones de anaerobiosis. Los resultados obtenidos demuestran que se puedo identificar Rothia dentocariosa en 6 pacientes del primer grupo (18 por ciento), en 3 pacientes del segundo grupo (9 por ciento), en tanto que no fue posible aislar dicha especie en la paciente sin caries dental. Cabe destacar que esta es la primera vez que se ha podido identificar a este microorganismos en nuestro país.
Descritores: Actinomycetales
Cárie Dentária
-Actinomycetales
Bioquímica/métodos
Contagem de Colônia Microbiana
Meios de Cultura
Placa Dentária
Faculdades de Odontologia/estatística & dados numéricos
Microscopia
Análise Estatística
Venezuela
Limites: Seres Humanos
Masculino
Adolescente
Adulto
Meia-Idade
Feminino
Responsável: AR29.1 - Biblioteca



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