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Texto completo SciELO Brasil
Texto completo
Id: lil-755811
Autor: Soleimani-Delfan, Abbas; Etemadifar, Zahra; Emtiazi, Giti; Bouzari, Majid.
Título: Isolation of Dickeya dadantii strains from potato disease and biocontrol by their bacteriophages
Fonte: Braz. j. microbiol;46(3):791-797, July-Sept. 2015. tab, ilus.
Idioma: en.

One of the most economically important bacterial pathogens of plants and plant products is Dickeya dadantii. This bacterium causes soft rot disease in tubers and other parts of the potato and other plants of the Solanaceae family. The application of restricted host range bacteriophages as biocontrol agents has recently gained widespread interest. This study purposed to isolate the infectious agent of the potato and evaluate its biocontrol by bacteriophages. Two phytopathogenic strains were isolated from infected potatoes, identified based on biochemical and 16S rRNA gene sequencing, and submitted to GenBank as D. dadantii strain pis3 (accession no. HQ423668) and D. dadantii strain sip4 (accession no. HQ423669). Their bacteriophages were isolated from Caspian Sea water by enriching the water filtrate with D. dadantii strains as hosts using spot or overlay methods. On the basis of morphotypes, the isolated bacteriophages were identified as members of the Myoviridae and Siphoviridae families and could inhibit the growth of antibiotic resistant D. dadantii strains in culture medium. Moreover, in Dickeya infected plants treated with bacteriophage, no disease progression was detected. No significant difference was seen between phage-treated and control plants. Thus, isolated bacteriophages can be suggested for the biocontrol of plant disease caused by Dickeya strains.

Descritores: Bacteriófagos/isolamento & purificação
Agentes de Controle Biológico/isolamento & purificação
Dickeya chrysanthemi/crescimento & desenvolvimento
Dickeya chrysanthemi/virologia
Doenças das Plantas/microbiologia
Solanum tuberosum/microbiologia
-Sequência de Bases
Agentes de Controle Biológico/classificação
DNA Bacteriano/genética
Testes de Sensibilidade Microbiana
Dados de Sequência Molecular
Myoviridae/isolamento & purificação
Dickeya chrysanthemi/efeitos dos fármacos
Dickeya chrysanthemi/isolamento & purificação
Análise de Sequência de DNA
Siphoviridae/isolamento & purificação
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME

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Texto completo SciELO Brasil
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Id: lil-684530
Autor: Brazilian Journal of Medical and Biological Research; Zanetti, C.C.S.; Mingrone, R.C.C.; Kisielius, J.J.; Ueda-Ito, M.; Pignatari, A.C.C..
Título: Characterization of bacteriophages infecting clinical isolates of Pseudomonas aeruginosa stored in a culture collection
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;46(8):689-695, ago. 2013. tab, graf.
Idioma: en.
Resumo: Some clinical isolates of Pseudomonas aeruginosa stored in our culture collection did not grow or grew poorly and showed lysis on the culture plates when removed from the collection and inoculated on MacConkey agar. One hypothesis was that bacteriophages had infected and killed those clinical isolates. To check the best storage conditions to maintain viable P. aeruginosa for a longer time, clinical isolates were stored at various temperatures and were grown monthly. We investigated the presence of phage in 10 clinical isolates of P. aeruginosa stored in our culture collection. Four strains of P. aeruginosa were infected by phages that were characterized by electron microscopy and isolated to assess their ability to infect. The best condition to maintain the viability of the strains during storage was in water at room temperature. Three Siphoviridae and two Myoviridae phages were visualized and characterized by morphology. We confirmed the presence of bacteriophages infecting clinical isolates, and their ability to infect and lyse alternative hosts. Strain PAO1, however, did not show lysis to any phage. Mucoid and multidrug resistant strains of P. aeruginosa showed lysis to 50% of the phages tested.
Descritores: Bacteriólise/fisiologia
Bacteriófagos/isolamento & purificação
Pseudomonas aeruginosa/virologia
-Técnicas Bacteriológicas
Bancos de Espécimes Biológicos
Meios de Cultura
Farmacorresistência Bacteriana Múltipla
Microscopia Eletrônica
Myoviridae/isolamento & purificação
Pseudomonas aeruginosa/classificação
Pseudomonas aeruginosa/isolamento & purificação
Siphoviridae/isolamento & purificação
Ensaio de Placa Viral
Limites: Humanos
Responsável: BR1.1 - BIREME

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