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Id: biblio-952839
Autor: Tamura, Rodrigo Esaki; de Luna, Igor Vieira; Lana, Marlous Gomes; Strauss, Bryan E.
Título: Improving adenoviral vectors and strategies for prostate cancer gene therapy
Fonte: Clinics;73(supl.1):e476s, 2018. graf.
Idioma: en.
Resumo: Gene therapy has been evaluated for the treatment of prostate cancer and includes the application of adenoviral vectors encoding a suicide gene or oncolytic adenoviruses that may be armed with a functional transgene. In parallel, versions of adenoviral vector expressing the p53 gene (Ad-p53) have been tested as treatments for head and neck squamous cell carcinoma and non-small cell lung cancer. Although Ad-p53 gene therapy has yielded some interesting results when applied to prostate cancer, it has not been widely explored, perhaps due to current limitations of the approach. To achieve better functionality, improvements in the gene transfer system and the therapeutic regimen may be required. We have developed adenoviral vectors whose transgene expression is controlled by a p53-responsive promoter, which creates a positive feedback mechanism when used to drive the expression of p53. Together with improvements that permit efficient transduction, this new approach was more effective than the use of traditional versions of Ad-p53 in killing prostate cancer cell lines and inhibiting tumor progression. Even so, gene therapy is not expected to replace traditional chemotherapy but should complement the standard of care. In fact, chemotherapy has been shown to assist in viral transduction and transgene expression. The cooperation between gene therapy and chemotherapy is expected to effectively kill tumor cells while permitting the use of reduced chemotherapy drug concentrations and, thus, lowering side effects. Therefore, the combination of gene therapy and chemotherapy may prove essential for the success of both approaches.
Descritores: Neoplasias da Próstata/terapia
Terapia Genética/métodos
Adenoviridae/genética
Carcinoma Pulmonar de Células não Pequenas/genética
Vetores Genéticos/uso terapêutico
Neoplasias Pulmonares/genética
-Neoplasias da Próstata/genética
Neoplasias da Próstata/imunologia
Proteína Supressora de Tumor p53/biossíntese
Antígeno Prostático Específico/genética
Genes Transgênicos Suicidas
Proteínas de Neoplasias/genética
Limites: Humanos
Masculino
Tipo de Publ: Research Support, Non-U.S. Gov't
Revisão
Responsável: BR1.1 - BIREME


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Texto completo SciELO Chile
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Id: biblio-1004341
Autor: Fica, Alberto; Cataldo, Pabla; Aceituno, Diana; Villarroel, María Ignacia; Escalona, Arturo.
Título: Fatal nosocomial hemorrhagic enterocolitis probably caused by adenovirus: report of one case / Enterocolitis hemorrágica fatal nosocomial probablemente causada por adenovirus: informe de un caso
Fonte: Rev. méd. Chile;147(2):256-260, Feb. 2019. graf.
Idioma: en.
Resumo: ABSTRACT Adenovirus (ADV) is a recognized cause of severe disease among immunocompromised patients. We report a previously healthy 39-year-old female, admitted with influenza pneumonia and evolving with lung hemorrhage and acute renal failure requiring mechanical ventilation and hemodialysis. She received high corticosteroid doses due to an initial suspicion of alveolar hemorrhage. Lymphopenia already present before steroid use (567/μL), was maintained during the whole hospital stay (mean 782/μL). From the second week of admission she presented a high-volume diarrhea (mean 2.5 L/day) associated to intermittent bloody stools. An ulcerative enterocolitis was confirmed by CT images and colonoscopy. ADV was detected in a colonic tissue sample by real time PCR but not by a commercial filmarray test. Cidofovir-probenecid and racecadotril therapy were indicated without changing the clinical course of diarrhea and the patient finally died.

Adenovirus (ADV) es una causa reconocida de enfermedades graves en pacientes inmunocomprometidos. Informamos el caso de una mujer de 39 años, previamente sana, que ingresó por neumonía grave por influenza, evolucionando con hemorragia pulmonar y falla renal aguda, requiriendo ventilación mecánica y hemodiálisis. Recibió altas dosis de corticoides por la sospecha inicial de una hemorragia alveolar. Tuvo linfopenia durante toda su estadía (promedio 782/μL), la que ya estaba presente antes del uso de los corticoides (567/μL). Desde la segunda semana de hospitalización, presentó una diarrea de alto volumen (promedio 2,5 L/día) asociada a la presencia de sangre en deposiciones en forma intermitente. Se confirmó una enterocolitis ulcerativa por tomografía computada y colonoscopía. Se detectó ADV en muestras de biopsia colónica por PCR en tiempo real pero no por un test de PCR múltiples automatizado comercial. Fue tratada con cidofovir-probenecid y racecadrotrilo sin impacto clínico y la paciente finalmente falleció.
Descritores: Infecção Hospitalar/etiologia
Hospedeiro Imunocomprometido
Infecções por Adenoviridae/complicações
Enterocolite/etiologia
Hemorragia Gastrointestinal/etiologia
-Adenoviridae/isolamento & purificação
Infecção Hospitalar/diagnóstico
Infecção Hospitalar/imunologia
Evolução Fatal
Infecções por Adenoviridae/microbiologia
Diarreia/complicações
Enterocolite/diagnóstico
Enterocolite/imunologia
Hemorragia Gastrointestinal/diagnóstico
Hemorragia Gastrointestinal/imunologia
Limites: Humanos
Feminino
Adulto
Tipo de Publ: Relatos de Casos
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Brasil
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Id: biblio-1131851
Autor: Nichols, Larry; Armstrong, Justin; Atkinson, Cody.
Título: The grim reaper evading modern medicine: aspergillosis, adenovirus, and Hodgkin lymphoma
Fonte: Autops. Case Rep;10(4):e2020191, 2020. graf.
Idioma: en.
Resumo: Illustrative cases of diseases that are difficult to suspect and diagnose can serve as useful reminders. Invasive pulmonary aspergillosis and adenovirus hepatitis are two such diseases, both revealed by autopsy in this case of Hodgkin lymphoma refractory to chemotherapy treated with allogeneic hematopoietic stem cell transplantation complicated by these two fatal infections. This patient was cured of Hodgkin lymphoma, Clostridioides difficile colitis and thrombotic thrombocytopenic purpura using the marvels of modern medicine. This case illustrates many features of aspergillosis and adenovirus hepatitis, shows the value of autopsy in revealing diagnoses, and illustrates the limits of modern medicine, which should serve as a mental spur in our efforts to advance medical science, to try to defeat the numerous demons of disease, who seem to keep outwitting us.
Descritores: Doença de Hodgkin/complicações
Adenoviridae
Aspergilose Pulmonar Invasiva/patologia
Hepatite
-Autopsia
Limites: Humanos
Masculino
Adulto
Tipo de Publ: Relatos de Casos
Responsável: BR26.7 - Serviço de Biblioteca e Documentação Científica


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Id: biblio-888889
Autor: Akçay, Emine; Çarhan, Ahmet; Hondur, Gözde; Tufan, Zeliha Koçak; Duru, Necati; Kılıç, Selçuk; Ensari, Ezgi Naz; Uğurlu, Nagihan; Çağıl, Nurullah.
Título: Molecular identification of viral agents associated with acute conjunctivitis: a prospective controlled study
Fonte: Braz. j. infect. dis;21(4):391-395, July-Aug. 2017. tab.
Idioma: en.
Resumo: Abstract Background: Viral conjunctivitis are the most frequent infections in ophthalmology clinics. The diagnosis is usually relying on clinical findings and medical history. However, topical antibiotics are often used unnecessarily addition to symptomatic treatment because of unsure agents. We aimed to detect the Adenovirus, Coxsackievirus and Enterovirus from conjunctiva and pharyngeal samples of patients. Methods: The conjunctiva and pharyngeal samples of the patients with conjunctivitis were taken by Virocult transport media and kept at -80 ºC up to study day. Adenovirus spp, Enterovirus 70 and Enterovirus 71, Coxsackie A24 and Coxsackie A16 were detected by real-time PCR. Samples from healthy health care workers of ophthalmology clinic were used for control group. Results: A total of 176 samples (conjunctival and pharyngeal samples of 62 patient and 26 healthy subjects) were included. The mean age of 34 (55.7%) male and 27 (44.3%) female patients was 34 ± 17. Twenty five (40.3%) of the patients were receiving antibiotic drops at first visit. The main etiologic agent in conjunctival samples was found to be Adenovirus (46/62, 74.2%) followed by Enterovirus 70 (4/62, 6.4%) and Enterovirus 71 (4/62, 6.4%). Coxsackievirus 16 and 24 were also found in 2 patients (1/62 each, 1.6%). Pharyngeal samples were also positive for Adenovirus (20/62, 32.3%), Enterovirus 70 and 71 (7/62, 11.3% and 5/62, 8.1% respectively), Coxsackievirus 16 and 24 (2/62, 3.2% and 1/61, 1.6%). Conclusions: It is very difficult in viral conjunctivitis to make clinical differentiation caused by different agents because of common clinical signs and symptoms. In routine clinical work, the viral conjunctivitis usually related with Adenovirus. But almost one fourth of the patients' conjunctivitis were not related to Adenovirus, which shows the importance of the laboratory diagnostics. True diagnosis plays an important role on prevention of contamination and unnecessary use of antibiotics in viral conjunctivitis.
Descritores: Faringe/virologia
DNA Viral/isolamento & purificação
Adenoviridae/isolamento & purificação
Conjuntivite Viral/virologia
Enterovirus/isolamento & purificação
-Estudos de Casos e Controles
Adenoviridae/classificação
Adenoviridae/genética
Reação em Cadeia da Polimerase
Doença Aguda
Estudos Prospectivos
Enterovirus/classificação
Enterovirus/genética
Limites: Humanos
Masculino
Feminino
Adulto
Tipo de Publ: Ensaio Clínico Controlado
Responsável: BR1.1 - BIREME


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Id: biblio-1120884
Autor: Veiga, Ana Beatriz Gorini da; Martins, Letícia Garay; Riediger, Irina; Mazetto, Alix; Debur, Maria do Carmo; Gregianini, Tatiana Schaffer.
Título: More than just a common cold: endemic coronaviruses OC43, HKU1, NL63, and 229E associated with severe acute respiratory infection and fatality cases among healthy adults
Fonte: J. med. virol;92(10):1-6, Aug. 2, 2020. tab.
Idioma: en.
Resumo: Respiratory viral infection can cause severe disease and hospitalization, especially among children, the elderly, and patients with comorbidities. In Brazil, the official surveillance system of severe acute respiratory infection (SARI) investigates influenza A (IAV) and B (IBV) viruses, respiratory syncytial virus (RSV), adenovirus (HAdV), and parainfluenza viruses (hPIV 1­3). In Rio Grande do Sul (RS), Brazil, many fatalities associated with SARI between 2013 and 2017 occurred among patients without underlying diseases and for whom the causative agent had not been identified using official protocols. This cross­sectional study analyzed the presence of coronaviruses (HCoV), bocavirus (HBoV), metapneumovirus (hMPV), and rhinovirus in patients who died of SARI despite not having comorbidities, and that were negative for IAV, IBV, RSV, HAdV, and hPIV. Nasopharyngeal aspirates/swabs from patients were used for nucleic acid extraction. The presence of HCoVs OC43, HKU1, NL63, and 229E; HBoV; hMPV; and rhinovirus was assessed by quantitative reverse transcription­polymerase chain reaction. Clinical data were also analyzed. Between 2013 and 2017, 16 225 cases of SARI were reported in RS; 9.8% of the patients died; 20% of all fatal cases were patients without comorbidities and for whom no pathogen was detected using standard protocols. Analysis of 271 of these cases identified HCoV in nine cases; HBoV, hMPV, and rhinovirus were detected in 3, 3, and 10 cases, respectively. Of note, patients infected with HCoV were adults. Results reinforce the importance of including coronaviruses in diagnostic panels used by official surveillance systems because besides their pandemic potential, endemic HCoVs are associated to severe disease in healthy adults.
Descritores: Sistema Respiratório
Coronavirus
Monitoramento Epidemiológico
Infecções
-Pacientes
Rhinovirus
Vírus
Viroses
Adenoviridae
Doença
Síndrome Respiratória Aguda Grave
Influenza Humana
Bocavirus
Limites: Humanos
Masculino
Feminino
Criança
Adolescente
Adulto
Pessoa de Meia-Idade
Idoso
Idoso de 80 Anos ou mais
Adulto Jovem
Responsável: BR1618.1 - CID - Centro de Informação e Documentação


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Id: biblio-1008601
Autor: Zhou, Guangxian; Yuan, Chao; He, Xiaolin; Kang, Danju; Wang, Xiaolong; Chen, Yulin.
Título: Effect of miR-125b on dermal papilla cells of goat secondary hair follicle
Fonte: Electron. j. biotechnol;25:64-69, ene. 2017. tab, graf, ilus.
Idioma: en.
Projeto: National Natural Science Foundation of China; . International Cooperation Project of Shaanxi Province.
Resumo: Background: MicroRNAs (miRNAs) are endogenous noncoding RNAs that regulate various biological processes. miR-125b is a miRNA that has been reported to be critical for hair follicle (HF) morphogenesis and development. We identified that the expression of miR-125b varies during an individual hair cycle (anagen, catagen, and telogen) in the skin of cashmere goats. We constructed a gain model (by overexpressing miR-125b) and a loss model (by inhibiting endogenous miR-125b) based on dermal papilla cells (DPCs) to further investigate the role of miR-125b in HF cycle. In addition, we used a dual-luciferase system to highlight the predicated target genes of miR-125b. Results: We found that miR-125b affects the expression of FGF5, IGF-1, SHH, TNF-α, MSX2, LEF-1, FGF7, NOGGIN, BMP2, BMP4, TGF-ß1, and ß-catenin. The dual-luciferase assay further validated a direct interaction between miR-125b and FGF5 and TNF-α. Conclusion: miR-125b affects the expression levels of genes related to hair cycle and may also play a critical role in regulating the periodic development of HF.
Descritores: Folículo Piloso/crescimento & desenvolvimento
MicroRNAs/metabolismo
-Recombinação Genética
Cabras
Adenoviridae
Fator de Necrose Tumoral alfa/metabolismo
Folículo Piloso/citologia
Folículo Piloso/metabolismo
MicroRNAs/genética
Fator 5 de Crescimento de Fibroblastos/metabolismo
Ensaios Enzimáticos
Luciferases
Limites: Animais
Responsável: CL1.1 - Biblioteca Central


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Id: lil-750792
Autor: Paiva, Terezinha Maria de.
Título: Use of molecular test for adenovirus detection between different pediatric patients / Aplicação de teste molecular para detecção de adenovírus em pacientes pediátricos distintos
Fonte: Rev. paul. pediatr;33(2):134-135, Apr-Jun/2015.
Idioma: en.
Descritores: Adenoviridae
Infecções por Adenoviridae
Limites: Humanos
Criança
Tipo de Publ: Comentário
Editorial
Responsável: BR1.1 - BIREME


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Id: biblio-970519
Autor: Oroña, M(edt); Chuffo, C(edt); Pereira, G(edt).
Título: Hemorragia digestiva / Gatrointestinal bleeding
Fonte: Med. infant;25(4):322-328, diciembre 2018. ilus.
Idioma: es.
Descritores: Pneumonia/etiologia
Púrpura Trombocitopênica Trombótica/diagnóstico
Adenoviridae/isolamento & purificação
Hemorragia Gastrointestinal/diagnóstico
Hemorragia Gastrointestinal/etiologia
Anemia Hemolítica/diagnóstico
-Diagnóstico Diferencial
Duodenopatias/complicações
Duodenopatias/diagnóstico
Limites: Humanos
Lactente
Tipo de Publ: Relatos de Casos
Responsável: AR305.1 - SID - Servicio de Información y Documentación


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Miagostovich, Marize Pereira
Leite, José Paulo Gagliardi
Texto completo
Id: lil-784253
Autor: Portes, Silvana Augusta Rodrigues; Volotão, Eduardo de Mello; Rocha, Monica Simões; Rebelo, Maria Cristina; Xavier, Maria da Penha Trindade Pinheiro; Assis, Rosane Maria de; Rose, Tatiana Lundgren; Miagostovich, Marize Pereira; Leite, José Paulo Gagliardi; Carvalho-Costa, Filipe Anibal.
Título: A non-enteric adenovirus A12 gastroenteritis outbreak in Rio de Janeiro, Brazil
Fonte: Mem. Inst. Oswaldo Cruz;111(6):403-406, June 2016. tab, graf.
Idioma: en.
Resumo: A gastroenteritis outbreak that occurred in 2013 in a low-income community in Rio de Janeiro was investigated for the presence of enteric viruses, including species A rotavirus (RVA), norovirus (NoV), astrovirus (HAstV), bocavirus (HBoV), aichivirus (AiV), and adenovirus (HAdV). Five of nine stool samples (83%) from patients were positive for HAdV, and no other enteric viruses were detected. Polymerase chain reaction products were sequenced and subjected to phylogenetic analysis, which revealed four strains and one strain of non-enteric HAdV-A12 and HAdV-F41, respectively. The HAdV-A12 nucleotide sequences shared 100% nucleotide similarity. Viral load was assessed using a TaqMan real-time PCR assay. Stool samples that were positive for HAdV-A12 had high viral loads (mean 1.9 X 107 DNA copies/g stool). All four patients with HAdV-A12 were < 25 months of age and had symptoms of fever and diarrhoea. Evaluation of enteric virus outbreaks allows the characterisation of novel or unique diarrhoea-associated viruses in regions where RVA vaccination is routinely performed.
Descritores: Infecções por Adenoviridae/epidemiologia
Adenoviridae/isolamento & purificação
Gastroenterite/virologia
-Infecções por Adenoviridae/virologia
Adenoviridae/genética
Brasil/epidemiologia
Diarreia/epidemiologia
Diarreia/virologia
Surtos de Doenças
Fezes/virologia
Gastroenterite/epidemiologia
Filogenia
Reação em Cadeia da Polimerase em Tempo Real
RNA Viral/genética
Limites: Humanos
Masculino
Feminino
Lactente
Pré-Escolar
Adulto
Pessoa de Meia-Idade
Responsável: BR1.1 - BIREME


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Id: lil-779174
Autor: Puchi S., Alexa; Karlsruher R., Gisela; Ramírez M., Francisca.
Título: Identificación de virus respiratorios en pacientes pediátricos / Identification of respiratory viruses in pediatric patients
Fonte: Bol. Hosp. Viña del Mar;70(2):61-66, jun.2014. graf.
Idioma: es.
Resumo: Los virus son la principal causa de infecciones respiratorias agudas. Para la detección de los virus respiratorios se emplea la Inmunofluorescencia directa (IFD). Objetivos: Describir los resultados de las IFD positivas según edad de los pacientes y estacionalidad y determinar el porcentaje de positividad. Material y métodos: Estudio descriptivo retrospectivo. Se incluyeron todas las IFD realizadas en pacientes <15 años en el Hospital de Niños de Viña del Mar entre enero 2012 y noviembre 2013. Resultados: Se realizaron 32015 IFD en 9012 pacientes en ambos años. El virus más frecuente es el Virus Respiratorio Sincicial (VRS) (51 por ciento 2012, 63 porciento 2013), obteniendo un mayor número de casos en pacientes menores de 1año. El Adenovirus (ADV) ocupa el segundo lugar con una incidencia uniforme a lo largo del año. Se observó una disminución de los casos de Influenza A y B desde el año 2012 al 2013. El porcentaje de positividad global de los exámenes es muy bajo (4,25 por ciento 2012 y 3,19 por ciento 2013). El VRS alcanza la mayor positividad en invierno (31,8 por ciento 2012 y24,7 por ciento 2013) y llega al 0 por ciento durante el verano. Conclusiones: Nuestros resultados se asemejan a la epidemiología nacional principalmente en la distribución de los virus según edad y estacionalidad. El virus más frecuentemente encontrado es el VRS. Hubo menos casos de Influenza A durante el 2013 en comparación con los resultados en el país. Existe una solicitud aumentada de exámenes lo que lleva a un porcentaje de positividad muy bajo...

The main cause of acute respiratory infections is viral. The direct immunofluorescence (IF) is used in the detection of respiratory viruses. Objectives: To describe the results of positive IF in relationship to the patient´s age and seasonality and to determine the percentage of positive results. Material and Methods: A descriptive retrospective study that includes all IF performed in patients <15 years old in a private clinic in the V region from January 2012 to November 2013. Results: 32015 IF were conducted in 9012 patients during both years. The Respiratory Syncytial Virus (RSV) is the most common virus (51 percent 2012, 63 percent 2013), obtaining a larger number of cases in patients under the age of 1. The second most common virus is Adenovirus (ADV) with a uniform incidence during the year. A decrease in cases of Influenza A and B was observed from 2012 to 2013. The percent positivity of the tests is very low (4.25 percent and 3.19 percent 2012 2013). The RSV positive testing is higher during the winter (31.8 percent and 24.7 percent 2012 2013) and 0 percent during the summer. Conclusions: Our results are similar to the ones obtained by the national epidemiology in terms of age and season relationship. The RSV is the most common virus. There were fewer cases of influenza A in 2013 compared with results in the country. Due to high demand in IF testing, a very low percentage of positive results is observed...
Descritores: Técnica Direta de Fluorescência para Anticorpo
Infecções por Vírus Respiratório Sincicial/diagnóstico
Infecções por Vírus Respiratório Sincicial/epidemiologia
-Distribuição por Idade
Adenoviridae/isolamento & purificação
Chile
Estudos Transversais
Epidemiologia Descritiva
Infecções por Adenoviridae/diagnóstico
Infecções por Adenoviridae/epidemiologia
Estações do Ano
Sensibilidade e Especificidade
Vírus Sinciciais Respiratórios/isolamento & purificação
Limites: Humanos
Lactente
Responsável: CL41.1 - Biblioteca Médica



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