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Id: biblio-887187
Autor: Santos, Mônica; Maquiné, Gustavo Ávila; Talhari, Carolina; Schettini, Antonio Pedro Mendes.
Título: Atypical crusted scabies in a patient with chronic liver disease caused by hepatitis B and D viruses
Fonte: An. bras. dermatol;93(2):277-278, Mar.-Apr. 2018. graf.
Idioma: en.
Resumo: Abstract: Crusted scabies is a less common variant of scabies that is highly contagious, difficult to treat and involves infestation by Sarcoptes scabiei var. hominis. The classical clinical presentation includes crusted, scaly and generally non-pruritic lesions usually located on the head, neck, palmar, plantar and periungual region. It was first described in Norway in 1848 in patients with leprosy who presented with crusted lesions. In this study, we report the case of a patient with crusted scabies with florid clinical manifestations and chronic liver disease due to hepatitis B and delta virus infection.
Descritores: Escabiose/patologia
Escabiose/tratamento farmacológico
Vírus Delta da Hepatite
Vírus da Hepatite B
Doença Hepática Terminal/virologia
-Escabiose/imunologia
Resultado do Tratamento
Doença Hepática Terminal/complicações
Antiparasitários/uso terapêutico
Limites: Seres Humanos
Masculino
Meia-Idade
Tipo de Publ: Relatos de Casos
Responsável: BR1.1 - BIREME


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Id: biblio-974300
Autor: Sundarrajan, Sudarson; Rao, Sneha; Padmanabhan, Sriram.
Título: Cloning and high-level expression of Thermus thermophilus RecA in E. coli: purification and novel use in HBV diagnostics
Fonte: Braz. j. microbiol;49(4):848-855, Oct.-Dec. 2018. tab, graf.
Idioma: en.
Resumo: ABSTRACT We studied the role of Thermus thermophilus Recombinase A (RecA) in enhancing the PCR signals of DNA viruses such as Hepatitis B virus (HBV). The RecA gene of a thermophilic eubacterial strain, T. thermophilus, was cloned and hyperexpressed in Escherichia coli. The recombinant RecA protein was purified using a single heat treatment step without the use of any chromatography steps, and the purified protein (>95%) was found to be active. The purified RecA could enhance the polymerase chain reaction (PCR) signals of HBV and improve the detection limit of the HBV diagnosis by real time PCR. The yield of recombinant RecA was ∼35 mg/L, the highest yield reported for a recombinant RecA to date. RecA can be successfully employed to enhance detection sensitivity for the diagnosis of DNA viruses such as HBV, and this methodology could be particularly useful for clinical samples with HBV viral loads of less than 10 IU/mL, which is interesting and novel.
Descritores: Proteínas de Bactérias/genética
Vírus da Hepatite B/isolamento & purificação
Reação em Cadeia da Polimerase/métodos
Thermus thermophilus/enzimologia
Clonagem Molecular
Recombinases/genética
-Proteínas de Bactérias/isolamento & purificação
Proteínas de Bactérias/metabolismo
Expressão Gênica
Vírus da Hepatite B/genética
Reação em Cadeia da Polimerase/instrumentação
Thermus thermophilus/genética
Recombinases/isolamento & purificação
Recombinases/metabolismo
Escherichia coli/genética
Escherichia coli/metabolismo
Responsável: BR1.1 - BIREME


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Id: biblio-828194
Autor: Rosca, Irina; Petrovici, Anca Roxana; Brebu, Mihai; Stoica, Irina; Minea, Bogdan; Marangoci, Narcisa.
Título: An original method for producing acetaldehyde and diacetyl by yeast fermentation
Fonte: Braz. j. microbiol;47(4):949-954, Oct.-Dec. 2016. tab, graf.
Idioma: en.
Projeto: \"Program of Excellency in the multidisciplinary doctoral and post-doctoral research of chronic diseases\"; . European Social Fund through the Sectoral Operational Program Human Resources Development; . Romanian National Authority for Scientific Research, CNCS - UEFISCDI.
Resumo: Abstract In this study a natural culture medium that mimics the synthetic yeast peptone glucose medium used for yeast fermentations was designed to screen and select yeasts capable of producing high levels of diacetyl and acetaldehyde. The presence of whey powder and sodium citrate in the medium along with manganese and magnesium sulfate enhanced both biomass and aroma development. A total of 52 yeasts strains were cultivated in two different culture media, namely, yeast peptone glucose medium and yeast acetaldehyde-diacetyl medium. The initial screening of the strains was based on the qualitative reaction of the acetaldehyde with Schiff's reagent (violet color) and diacetyl with Brady's reagent (yellow precipitate). The fermented culture media of 10 yeast strains were subsequently analyzed by gas chromatography to quantify the concentration of acetaldehyde and diacetyl synthesized. Total titratable acidity values indicated that a total titratable acidity of 5.5 °SH, implying culture medium at basic pH, was more favorable for the acetaldehyde biosynthesis using strain D15 (Candida lipolytica; 96.05 mg L-1 acetaldehyde) while a total titratable acidity value of 7 °SH facilitated diacetyl flavor synthesis by strain D38 (Candida globosa; 3.58 mg L-1 diacetyl). Importantly, the results presented here suggest that this can be potentially used in the baking industry.
Descritores: Vírus da Hepatite B/genética
Hepatite C/diagnóstico
Hepatite C/virologia
Hepacivirus/genética
Carga Viral
Hepatite B/diagnóstico
Hepatite B/virologia
-DNA Viral
RNA Viral
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
Reação em Cadeia da Polimerase em Tempo Real
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-828211
Autor: Zauli, Danielle Alves Gomes; Menezes, Carla Lisandre Paula de; Oliveira, Cristiane Lommez de; Mateo, Elvis Cristian Cueva; Ferreira, Alessandro Clayton de Souza.
Título: In-house quantitative real-time PCR for the diagnosis of hepatitis B virus and hepatitis C virus infections
Fonte: Braz. j. microbiol;47(4):987-992, Oct.-Dec. 2016. tab, graf.
Idioma: en.
Resumo: Abstract The quantification of viral nucleic acids in serum by real-time PCR plays an important role in diagnosing hepatitis B virus and hepatitis C virus infection. In this study, we developed an assay using specific primers and probes to quantify hepatitis B virus DNA or hepatitis C virus RNA in serum from infected patients. For standardization and validation of the assay, an international panel of hepatitis B virus/hepatitis C virus and standard plasmids was used. A correlation coefficient of 0.983 and 0.963 for hepatitis B virus and hepatitis C virus, respectively, was obtained based on cycle threshold values and concentrations of DNA or RNA. The standard curve showed a linear relationship from 19 IU/mL to 1.9 × 109 IU/mL of serum, with a coefficient of determination (r2) of 0.99. In sera from patients infected with hepatitis B virus or hepatitis C virus viral loads (19 IU/mL and 1.9 × 109 IU/mL), we quantified viral loads with a detection limit of 1.9 × 102 IU/mL. The real-time quantitative PCR assay developed in this study provides an ideal system for routine diagnosis and confirmation of indeterminate serological results, especially in immunosuppressed patients.
Descritores: Vírus da Hepatite B/genética
Hepatite C/diagnóstico
Hepatite C/virologia
Hepacivirus/genética
Carga Viral
Hepatite B/diagnóstico
Hepatite B/virologia
-DNA Viral
RNA Viral
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
Reação em Cadeia da Polimerase em Tempo Real
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


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Id: lil-618662
Autor: Rovira, Cristina; Picagua, Estela; Martínez, C; Ferreira, L; Granado, E; Giménez, Vivian; Machuca, M; Carpinelli, MM.
Título: Hepatitis B: antígeno de superficie y anticuerpos post vacunales en mujeres del hospital psiquiátrico de Asunción, Paraguay / Hepatitis B: surface antigen and post-vaccination antibodies in women from the psychiatric hospital of Asunción, Paraguay
Fonte: Mem. Inst. Invest. Cienc. Salud (Impr.);9(2):57-63, dic. 2011. tab.
Idioma: es.
Resumo: El virus de la Hepatitis B se transmite por diversas vías incluyendo la sanguínea, vertical y sexual. Pacientes con severas condiciones mentales están sometidos a mayor riesgo de adquirir cualquier infección viral como virus de hepatitis B, C y VIH. La duración de la estadía en las instituciones mentales tiene influencia en el contagio de la enfermedad y largos periodos de permanencia, incrementan la oportunidad para una transmisión viral. El objetivo fue conocer la frecuencia de antígeno de superficie de la hepatitis B y determinar los anticuerpos post vacunales contra la hepatitis en mujeres en edad reproductiva, internadas en un Hospital Psiquiátrico del Ministerio de Salud Pública y Bienestar Social de Asunción- Paraguay. Estudio observacional descriptivo llevado a cabo de mayo 2008 a mayo 2010. Se estudiaron 72 pacientes de sexo femenino, en edad fértil, de 18 a 49 años (promedio de 38 años ± 6). Los sueros fueron analizados para detectar: HBsAg basal y anti-HBs post vacunal por el método de ELISA. En las 72 pacientes estudiadas no se encontró evidencia serológica de la infección por virus de hepatitis B. De las 30 pacientes a quienes se les aplicó 3 dosis de vacuna, 29 presentaron valores protectivos adecuados (mayor a 20 mUI/mL) a los dos meses posteriores a la vacunación, una tuvo un valor inferior a 10 mUI/mL. Con la respuesta obtenida en las pacientes estudiadas en esta institución psiquiátrica, se estaría reduciendo las complicaciones de la hepatitis B e impidiendo la transmisión horizontal a las demás pacientes y al personal de salud de la institución y sobretodo se prevendrá la transmisión de hepatitis B al feto, en casos de embarazo.

Hepatitis virus B is transmitted by various routes including blood, vertical and sexual routes. Patients with severe mental health conditions are at greatest risk of acquiring any viral infection such as hepatitis B, C and HIV. The length of the stay in mental institutions influences the spread of the diseases and long periods of stay increase the opportunity for viral transmission. The objective of this study was to know the frequency of the surface antigen of hepatitis B and determine the post-vaccination antibodies against hepatitis in women of reproductive age, hospitalized in a psychiatric hospital of the Ministry of Public Health and Social Welfare of Asunción, Paraguay. This was an observational descriptive study carried out from May 2008 to May 2010. We studied 72 female patients in childbearing age, from 18 to 49 years (mean 38 years ± 6). Sera were tested for HBsAg and post-vaccination anti-HBs by ELISA. Serological evidence of Hepatitis B virus infection was not found in the 72 patients. Twenty nine out of thirty patients who were administered 3 doses of vaccine had adequate protective values (greater than 20 mIU/mL) at two months after vaccination and one had a value less than 10 mIU/mL. With the response found in the study patients of this mental health institution, the complications of hepatitis B would be reduced and the horizontal transmission to other patients and health personnel of the institution would be prevented and specially the hepatitis B transmission to fetus in case of pregnancy.
Descritores: Anticorpos Anti-Hepatite B
Hepatite B
Saúde Mental
Vírus da Hepatite B
Limites: Feminino
Responsável: PY3.1 - Biblioteca


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Id: biblio-869053
Autor: Olmedo, Gladys Beatriz; Zorrilla, María Elena; Bobadilla, María Liz; Villagra, Verónica; Avalos, Domingo Santiago; Huber, Claudia; Zarza, Natalia.
Título: Serorreactividad al antígeno de superficie del virus de la hepatitis B en mujeres que acudieron al laboratorio central de salud pública. Asunción, Paraguay / Seroreactivity against the hepatitis B virus surface antigen in women who attended the central public health laboratory. Asunción, Paraguay
Fonte: Mem. Inst. Invest. Cienc. Salud (Impr.);13(3):96-102, dic. 2015. tab.
Idioma: es.
Resumo: La hepatitis B es un grave problema de salud pública a nivel mundial, aproximadamente cerca de 2 billones de personas tienen evidencia serológica de infección por el virus de la hepatitis B. El objetivo de este trabajo fue describir la frecuencia de hepatitis B e identificar los factores de riesgo asociados en mujeres en edad fértil que acudieron al Laboratorio Central de Salud Pública entre diciembre de 2013 y junio de 2014. Fue un estudio observacional analítico de corte transverso que, previo consentimiento informado, analizó suero de mujeres entre 15 y 44 años con una edad promedio de 26,6 (±6,8) años. Mediante la detección del antígeno de superficie de la hepatitis B por ELISA se identificaron seis casos positivos (0,4%), indicando una endemicidad baja; cifra que ha variado según perfil socio demográfico: según edad, las de 20 y más años presentaron una frecuencia mayor en comparación a las demás (p>0,05). No se observaron diferencias significativas al evaluar la seropositividad según el estado civil, el nivel de escolaridad, la condición de gravidez, los antecedentes de transfusiones, sin embargo, la seropositividad era mayor en las portadoras de tatuajes/piercing que entre las no portadoras, lo que representaba un riesgo 6,2 veces mayor (OR:6,2 IC95%:1,3-31,3). En conclusión, la frecuencia del HBsAg en nuestra población es baja, y el factor de riesgo asociado a su detección fue la presencia de tatuajes y/o piercing.

Hepatitis B is a serious public health problem worldwide; approximately about 2 billionpeople have serologic evidence of infection with hepatitis B virus. The aim of this analyticcross-sectional study was to describe the frequency of hepatitis B and identify risk factorsin women of child bearing age who attended the Central Public Health Laboratory in theperiod 2013 to 2014. Prior informed consent, antigen detection of hepatitis B surface wasperformed by ELISA in women between 15 and 44 years with a mean age of 26.6 (±6.8)years. The identification of six serologic positive cases (0.4%) indicates low endemicity.This figure varied according to socio-demographic profile: according to age, those whowere 20 years old or older had an increased frequency compared to the others (p> 0.05). No significant differences were observed in seropositivity by marital status, level ofeducation, pregnancy, history of transfusion, while seropositivity was higher amongcarriers of tattoos/piercing than among non-carriers, which represented a 6.2 times higherrisk (OR 6.2 95% CI 1.3 to 31.3). In conclusion, the frequency of HBsAg in our populationwas low. The risk factor associated with its detection was the presence of tattoos and / or piercings.
Descritores: Hepatite B
Vírus da Hepatite B
-Saúde Pública
Limites: Seres Humanos
Adulto
Feminino
Meia-Idade
Responsável: PY3.1 - Biblioteca


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Id: biblio-969104
Autor: Perú. Ministerio de Salud; .Dirección General de Intervenciones Estratégicas en Salud Pública.
Título: Norma técnica de salud para la prevención, diagnóstico y tratamiento de la hepatitis viral B en el Perú / Technical health standard for the prevention, diagnosis and treatment of viral hepatitis B in Peru.
Fonte: LIma; Perú. Ministerio de Salud; 20181200. 65 p p. ilus.
Idioma: es.
Resumo: La norma contiene la finalidad y justificación técnica, ámbito de aplicación, base legal y disposiciones generales y específicas para la prevención, diagnóstico y tratamiento de la hepatitis viral B en el Perú.
Descritores: Vírus da Hepatite B
-Diagnóstico
Prevenção de Doenças
Normas Técnicas
Responsável: PE18.1 - Biblioteca Central


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Lemos, Marcílio Figueiredo
Moreira, Regina Celia
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Id: lil-772614
Autor: Santos, Ana Paula de Torres; Levi, José Eduardo; Lemos, Marcilio Figueiredo; Calux, Samira Julien; Oba, Isabel Takano; Moreira, Regina Célia.
Título: An in-house real-time polymerase chain reaction: standardisation and comparison with the Cobas Amplicor HBV monitor and Cobas AmpliPrep/Cobas TaqMan HBV tests for the quantification of hepatitis B virus DNA
Fonte: Mem. Inst. Oswaldo Cruz;111(2):134-140, Feb. 2016. tab, graf.
Idioma: en.
Projeto: FAPESP.
Resumo: This study aimed to standardise an in-house real-time polymerase chain reaction (rtPCR) to allow quantification of hepatitis B virus (HBV) DNA in serum or plasma samples, and to compare this method with two commercial assays, the Cobas Amplicor HBV monitor and the Cobas AmpliPrep/Cobas TaqMan HBV test. Samples from 397 patients from the state of São Paulo were analysed by all three methods. Fifty-two samples were from patients who were human immunodeficiency virus and hepatitis C virus positive, but HBV negative. Genotypes were characterised, and the viral load was measure in each sample. The in-house rtPCR showed an excellent success rate compared with commercial tests; inter-assay and intra-assay coefficients correlated with commercial tests (r = 0.96 and r = 0.913, p < 0.001) and the in-house test showed no genotype-dependent differences in detection and quantification rates. The in-house assay tested in this study could be used for screening and quantifying HBV DNA in order to monitor patients during therapy.
Descritores: DNA Viral/isolamento & purificação
Técnicas de Genotipagem/normas
Vírus da Hepatite B/isolamento & purificação
Hepatite B Crônica/diagnóstico
Técnicas de Diagnóstico Molecular
Reação em Cadeia da Polimerase em Tempo Real/normas
-Primers do DNA/normas
Estudos de Avaliação como Assunto
Genótipo
Soropositividade para HIV/sangue
Soropositividade para HIV/diagnóstico
Vírus da Hepatite B/genética
Hepatite B Crônica/sangue
Hepatite C/sangue
Hepatite C/diagnóstico
Invenções/normas
Técnicas de Diagnóstico Molecular/instrumentação
Técnicas de Diagnóstico Molecular/métodos
Sensibilidade e Especificidade
Carga Viral
Limites: Seres Humanos
Masculino
Feminino
Lactente
Pré-Escolar
Criança
Adolescente
Adulto
Meia-Idade
Idoso
Idoso de 80 Anos ou mais
Adulto Jovem
Tipo de Publ: Research Support, Non-U.S. Gov't
Estudo Comparativo
Responsável: BR1.1 - BIREME


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Villanova, Marcia Guimaräes
Martinelli, Ana de Lourdes Candolo
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Id: biblio-894874
Autor: Chachá, Silvana Gama Florencio; Gomes-Gouvêa, Michele Soares; Malta, Fernanda de Mello; Ferreira, Sandro da Costa; Villanova, Márcia Guimarães; Souza, Fernanda Fernandes; Teixeira, Andreza Correa; Passos, Afonso Dinis da Costa; Pinho, João Renato Rebello; Martinelli, Ana de Lourdes Candolo.
Título: Basal core promoter and precore mutations among hepatitis B virus circulating in Brazil and its association with severe forms of hepatic diseases
Fonte: Mem. Inst. Oswaldo Cruz;112(9):626-631, Sept. 2017. tab.
Idioma: en.
Projeto: FAPESP; . FAPESP; . FAPESP.
Resumo: BACKGROUND In Brazil, few studies have investigated the prevalence of infection with the precore (PC) and basal core promoter (BCP) mutants of the hepatitis B virus (HBV). OBJECTIVES This study aimed to analyse the frequency of PC and BCP mutations among patients infected with HBV and to evaluate the association between the variants and advanced hepatic disease. METHODS A total of 161 patients infected with HBV were studied. To identify PC and BCP mutations, a 501-bp fragment of HBV DNA was amplified and sequenced. FINDINGS PC and BCP regions from HBV strains were successfully amplified and sequenced in 129 and 118 cases, respectively. PC and BCP mutations were detected in 61.0% and 80.6% of the cases, respectively. The A1762T/G1764A variant was identified in 36.7% of the patients with grade 1 and 2 liver fibrosis (29/79) and in 81.8% of the patients with grade 3 and 4 liver fibrosis (9/11) (p < 0.01); in 76.9% of the patients with cirrhosis (10/13) and in 38.1% of the patients without cirrhosis (40/105) (p = 0.01); and in 77.8% of the patients with hepatocellular carcinoma (HCC) (7/9) and in 39.4% of the patients without HCC (43/109) (p = 0.03). MAIN CONCLUSIONS A high prevalence of HBV PC and BCP mutants was found. The A1762T/G1764A variant was independently associated with advanced forms of liver fibrosis, hepatic cirrhosis, and HCC.
Descritores: Proteínas do Core Viral/genética
Vírus da Hepatite B/genética
Hepatite B Crônica/virologia
Cirrose Hepática/virologia
-Genótipo
Mutação
Limites: Seres Humanos
Feminino
Adulto
Meia-Idade
Idoso
Idoso de 80 Anos ou mais
Responsável: BR1.1 - BIREME


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Martins, Regina Maria Bringel
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Id: lil-582746
Autor: Anjos, Giulena Rosa Leite Cardoso dos; Martins, Regina Maria Bringel; Carneiro, Megmar Aparecida dos Santos; Brunini, Sandra Maria; Teles, Sheila Araujo.
Título: Epidemiology of hepatitis B virus infection in first-time blood donors in the southwestern region of Goiás, central Brazil
Fonte: Rev. bras. hematol. hemoter;33(1):38-42, Feb. 2011. tab.
Idioma: en.
Resumo: INTRODUCTION: Little is known about the epidemiology of hepatitis B virus (HBV) infection in populations from inner cities, especially in Central Brazil. Thus the objective of this study was to estimate the prevalence of HBV infection, and to analyze the factors associated with HBV infection, in a population of first-time blood donors in the southwestern region of Goiás, Central Brazil. METHODS: A total of 984 individuals were interviewed and gave blood samples to detect serological markers of HBV (HBsAg, anti-HBs, and anti-HBc) by enzyme linked immunosorbent assays. RESULTS: An overall prevalence of 6.9 percent was found for HBV, with constituent prevalence rates of 3.6 percent and 11.6 percent, in subjects classified as fit and unfit to donate blood according the epidemiological screening, respectively. Only three individuals were positive for anti-HBs alone, suggesting previous vaccination against HBV. The variables of prior blood transfusion (OR = 2.3), tattoo/piercing (OR = 2.1), illicit drug use (OR = 2.3), sex with a partner with hepatitis (OR = 14.7), and history of sexually transmitted diseases (OR = 2.9) were independently associated with HBV-positivity. These data suggested a low endemicity of hepatitis B in the studied population. CONCLUSION: The findings of low hepatitis B immunization coverage and the association of hepatitis B with risky behavior highlight that there is a need to intensify hepatitis B prevention programs in the southwest region of Goiás.
Descritores: Brasil
Vírus da Hepatite B
Prevalência
Hepatite B/epidemiologia
Limites: Seres Humanos
Masculino
Feminino
Adolescente
Adulto
Meia-Idade
Idoso
Responsável: BR408.1 - Biblioteca da Faculdade de Medicina - BFM



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