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Texto completo SciELO Brasil
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Id: biblio-984761
Autor: Cataneo, Allan Henrique Depieri; Kuczera, Diogo; Mosimann, Ana Luiza Pamplona; Silva, Emanuele Guimarães; Ferreira, Álvaro Gil Araújo; Marques, João Trindade; Wowk, Pryscilla Fanini; Santos, Claudia Nunes Duarte dos; Bordignon, Juliano.
Título: Detection and clearance of a mosquito densovirus contaminant from laboratory stocks of Zika virus
Fonte: Mem. Inst. Oswaldo Cruz;114:e180432, 2019. tab, graf.
Idioma: en.
Projeto: Ministério da Saúde; . Fundação Araucária; . Fundação Araucária; . SESA-PR; . CNPq; . MS-Decit; . PPSUS; . JTM; . CNDS; . JB; . CNPq; . AHDC; . DK; . CNDS; . JB.
Resumo: BACKGROUND The Zika virus (ZIKV) epidemics that affected South America in 2016 raised several research questions and prompted an increase in studies in the field. The transient and low viraemia observed in the course of ZIKV infection is a challenge for viral isolation from patient serum, which leads to many laboratories around the world sharing viral strains for their studies. C6/36 cells derived from Aedes albopictus larvae are commonly used for arbovirus isolation from clinical samples and for the preparation of viral stocks. OBJECTIVES Here, we report the contamination of two widely used ZIKV strains by Brevidensovirus, here designated as mosquito densovirus (MDV). METHODS Molecular and immunological techniques were used to analyse the MDV contamination of ZIKV stocks. Also, virus passages in mammalian cell line and infecting susceptible mice were used to MDV clearance from ZIKV stocks. FINDINGS MDV contamination was confirmed by molecular and immunological techniques and likely originated from C6/36 cultures commonly used to grow viral stocks. We applied two protocols that successfully eliminated MDV contamination from ZIKV stocks, and these protocols can be widely applied in the field. As MDV does not infect vertebrate cells, we performed serial passages of contaminated stocks using a mammalian cell line and infecting susceptible mice prior to re-isolating ZIKV from the animals' blood serum. MDV elimination was confirmed with immunostaining, polymerase chain reaction (PCR), and analysis of the mosquitoes that were allowed to feed on the infected mice. MAIN CONCLUSIONS Since the putative impact of viral contaminants in ZIKV strains generally used for research purposes is unknown, researchers working in the field must be aware of potential contaminants and test viral stocks to certify sample purity.
Descritores: Cultura de Vírus
Bancos de Espécimes Biológicos
Zika virus
-DNA Viral
Imunofluorescência
Densovirus/genética
Camundongos
Limites: Humanos
Animais
Responsável: BR1.1 - BIREME


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Id: lil-589036
Autor: Mosimann, Ana Luiza Pamplona; Bordignon, Juliano; Mazzarotto, Giovanny Camacho Antevêre; Motta, Maria Cristina M; Hoffmann, Federico; Santos, Claudia Nunes Duarte dos.
Título: Genetic and biological characterization of a densovirus isolate that affects dengue virus infection
Fonte: Mem. Inst. Oswaldo Cruz;106(3):285-292, May 2011. ilus, tab.
Idioma: en.
Resumo: Brevidensoviruses have an encapsidated, single-stranded DNA genome that predominantly has a negative polarity. In recent years, they have received particular attention due to their potential role in the biological control of pathogenic arboviruses and to their unnoticed presence in cell cultures as contaminants. In addition, brevidensoviruses may also be useful as viral vectors. This study describes the first genetic and biological characterization of a mosquito densovirus that was isolated in Brazil; moreover, we examined the phylogenetic relationship between this isolate and the other brevidensoviruses. We further demonstrate that this densovirus has the potential to be used to biologically control dengue virus (DENV) infection with in vitro co-infection experiments. The present study provides evidence that this densovirus isolate is a fast-spreading virus that affects cell growth and DENV infection.
Descritores: Vírus da Dengue/crescimento & desenvolvimento
Densovirus
-Chlorocebus aethiops
Densovirus/fisiologia
Camundongos Endogâmicos BALB C
Controle Biológico de Vetores
Filogenia
Limites: Animais
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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