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Id: lil-680770
Autor: Memorias do Instituto Oswaldo Cruz; Salgado, Ana Paula Carneiro; Soares-Martins, Jamaria Adriana Pinheiro; Andrade, Luciana Garcia; Albarnaz, Jonas Dutra; Ferreira, Paulo Cesar Peregrino; Kroon, Erna Geessien; Bonjardim, Claudio Antonio.
Título: Study of Vaccinia and Cowpox viruses' replication in Rac1-N17 dominant-negative cells
Fonte: Mem. Inst. Oswaldo Cruz;108(5):554-562, ago. 2013. graf.
Idioma: en.
Resumo: Interfering with cellular signal transduction pathways is a common strategy used by many viruses to create a propitious intracellular environment for an efficient replication. Our group has been studying cellular signalling pathways activated by the orthopoxviruses Vaccinia (VACV) and Cowpox (CPXV) and their significance to viral replication. In the present study our aim was to investigate whether the GTPase Rac1 was an upstream signal that led to the activation of MEK/ERK1/2, JNK1/2 or Akt pathways upon VACV or CPXV' infections. Therefore, we generated stable murine fibroblasts exhibiting negative dominance to Rac1-N17 to evaluate viral growth and the phosphorylation status of ERK1/2, JNK1/2 and Akt. Our results demonstrated that VACV replication, but not CPXV, was affected in dominant-negative (DN) Rac1-N17 cell lines in which viral yield was reduced in about 10-fold. Viral late gene expression, but not early, was also reduced. Furthermore, our data showed that Akt phosphorylation was diminished upon VACV infection in DN Rac1-N17 cells, suggesting that Rac1 participates in the phosphoinositide-3 kinase pathway leading to the activation of Akt. In conclusion, our results indicate that while Rac1 indeed plays a role in VACV biology, perhaps another GTPase may be involved in CPXV replication.
Descritores: Vírus da Varíola Bovina/fisiologia
Sistema de Sinalização das MAP Quinases/fisiologia
Transdução de Sinais/fisiologia
Vírus Vaccinia/fisiologia
Replicação Viral/fisiologia
Proteínas rac1 de Ligação ao GTP/fisiologia
-BALB ABATTOIRSTABATTOIRS CELLS
Chlorocebus aethiops
Fosforilação/fisiologia
Células Vero
Proteínas rac1 de Ligação ao GTP/metabolismo
Limites: Animais
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-119750
Autor: Ledermann D., Walter.
Título: ¿Se acuerda de Jenner? / Do you remember Jenner?
Fonte: Rev. chil. infectol;8(2):93-5, 1991.
Idioma: es.
Descritores: Vírus da Varíola Bovina
História da Medicina
Vacina Antivariólica/história
Limites: Humanos
Tipo de Publ: Artigo Histórico
Responsável: CL1.1 - Biblioteca Central



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