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Id: biblio-1250822
Autor: Caterino-de-Araujo, Adele; Barbosa-Stancioli, Edel Figueiredo; Alonso Neto, José Boullosa; Aragón, Mayra Gonçalves; Galvão-Castro, Bernardo; Ishak, Ricardo; Rosadas, Carolina.
Título: Laboratory diagnosis of human T-lymphotropic virus in Brazil: assays, flowcharts, challenges, and perspectives
Fonte: Rev. Soc. Bras. Med. Trop;54:e01752021, 2021. tab, graf.
Idioma: en.
Projeto: CNPq.
Resumo: Abstract INTRODUCTION We present a data analysis and review of recent studies regarding the laboratory diagnosis of human T-lymphotropic virus 1 and 2 (HTLV-1/2) infections in Brazil. METHODS Target populations, available diagnostic serological assays (screening and complementary tests), molecular assays (in-house), causes of false-positive and false-negative results, and flowcharts were analyzed. RESULTS A table presents the target populations, two diagnostic flowcharts (depending on laboratory infrastructure and study population), and recent research that may improve how HTLV-1/2 is diagnosed in Brazil. CONCLUSIONS: Our results support the implementation of public policies to reduce HTLV-1/2 transmission and its associated diseases.
Descritores: Vírus Linfotrópico T Tipo 1 Humano
Infecções por HTLV-I/diagnóstico
Infecções por HTLV-II/diagnóstico
Técnicas de Laboratório Clínico
-Design de Software
Brasil
Vírus Linfotrópico T Tipo 2 Humano
Infecções por HTLV-II/epidemiologia
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-1041529
Autor: Ribeiro, Mirela Lopes; Gonçales, Juliana Prado; Morais, Viviane Martha Santos de; Moura, Libia Cristina Rocha Vilela; Coêlho, Maria Rosângela Cunha Duarte.
Título: HTLV 1/2 Prevalence and risk factors in individuals with HIV/AIDS in Pernambuco, Brazil
Fonte: Rev. Soc. Bras. Med. Trop;52:e20180244, 2019. tab.
Idioma: en.
Resumo: Abstract INTRODUCTION: Apart from masking the diagnosis of AIDS in patients with HIV/AIDS, human T-cell lymphotropic virus (HTLV), when present, also increases the risk of myelopathies and neurological disease in these patients. METHODS: Disease prevalence was estimated by ELISA and confirmed by Western blot. RESULTS: The coinfection rate was 1.5% (11/720); 10 of 11 patients had HTLV-1, and the remaining one had HTLV-2. The majority were male, over 40 years old, and of pardo color (ethnicity). CONCLUSIONS: There was no association between the risk factors examined and HTLV/HIV coinfection. This is the first study to report the occurrence of HTLV-2 in Pernambuco.
Descritores: Infecções por HTLV-I/complicações
Infecções por HTLV-II/complicações
Infecções por HIV/complicações
Coinfecção
-Fatores Socioeconômicos
Brasil/epidemiologia
Ensaio de Imunoadsorção Enzimática
Vírus Linfotrópico T Tipo 1 Humano
Infecções por HTLV-I/epidemiologia
Vírus Linfotrópico T Tipo 2 Humano
Infecções por HTLV-II/epidemiologia
Infecções por HIV/epidemiologia
Western Blotting
Prevalência
Fatores de Risco
Pessoa de Meia-Idade
Limites: Humanos
Masculino
Feminino
Adolescente
Adulto
Adulto Jovem
Responsável: BR1.1 - BIREME


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Id: biblio-1136919
Autor: Pontes, Gemilson Soares; Ribeiro, Hygor Halyson Figueiredo; Toro, Diana Mota; Moura Neto, José Pereira de; Souza, Victor; Almeida, Maria Edilene Martins de; Nascimento, Valdinete Alves do; Costa, Cristovão Alves da; Naveca, Felipe Gomes; Santos, Mike; Vallinoto, Antonio Carlos Rosário.
Título: HTLV-2 infection in Manaus, Brazil: first description of HTLV-2c subtype in an urban area of the Western Amazon region
Fonte: Rev. Soc. Bras. Med. Trop;54:e20200066, 2021. tab, graf.
Idioma: en.
Projeto: PROCAD AMAZONIA; . Conselho Nacional de Desenvolvimento Científico e Tecnológico.
Resumo: Abstract INTRODUCTION: We investigated the prevalence of human T-cell lymphotropic virus types 1 and 2 (HTLV-1/2) infection in patients with hematological diseases from the western Amazon region of Brazil. METHODS: Samples from 306 patients were submitted for the molecular diagnosis of HTLV-1/2 infection by real time PCR (qPCR), with amplification, sequencing, and phylogenetic analysis of the long terminal repeat (LTR) region. RESULTS: A 29-year-old male carrier of sickle cell anemia with a history of multiple blood transfusions was diagnosed with the HTLV-2c subtype. CONCLUSIONS: This study describes the first known occurrence of HTLV-2c in the urban area of Brazil's western Amazon region.
Descritores: Vírus Linfotrópico T Tipo 1 Humano/genética
Infecções por HTLV-I/diagnóstico
Infecções por HTLV-I/epidemiologia
Infecções por HTLV-II/diagnóstico
Infecções por HTLV-II/epidemiologia
-Filogenia
Brasil/epidemiologia
Vírus Linfotrópico T Tipo 2 Humano/genética
Limites: Humanos
Masculino
Gravidez
Adulto
Tipo de Publ: Relatos de Casos
Responsável: BR1.1 - BIREME


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Mochel, Elba Gomide
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Id: lil-763883
Autor: Souza, Verônica Guimarães de; Silva, Camila Moreira Serra e; Pires, Claudyene; Mochel, Elba Gomide; Martins, Christiane de Sousa; Gomes, Samea Cristina.
Título: Perfil de gestantes submetidas à triagem do vírus HTLV no Maranhão / Profile of pregnant women submitted to screening for HTLV - Maranhão / Perfil de gestantes sometidas a la detección de virus HTLV ? en Maranhão
Fonte: Rev. baiana enferm;25(1), 2011.
Idioma: pt.
Resumo: O objetivo deste artigo é avaliar o perfil epidemiológico das gestantes submetidas à triagem para o vírus HTLV1 e 2 atendidas no pré-natal de três serviços públicos. Trata-se de estudo transversal realizado entre 11/2/2008 e3/12/2008, com 2.044 gestantes em três serviços públicos de pré-natal, São Luis (MA). A amostra foi calculada em2.041, poder do teste 95%, erro absoluto de 5%. Foi usado o programa Stata 9.0, realizado teste qui-quadrado eaceito p<0,05 (95%) como limite para significância. Os resultados informam que a média de idade foi de 25 anos;35,12% são solteiras com companheiros; 41,10% de cor da pele parda; 57,19% com ensino médio completo; 54,21%iniciaram atividade sexual entre 15 e 18 anos; 35,08% nunca usaram preservativo em relações sexuais; 54,99% sãoprimigestas; 15,22% foram amamentadas pelas mães e outras pessoas; 42,51% já tiveram filhos, destas 31,90% jápraticaram aleitamento cruzado; e 1,2% já usaram drogas ilícitas. Conclui-se que as gestantes apresentam característicasque justificam a triagem do vírus HTLV 1 e 2 durante o pré-natal, no entanto são necessários mais estudospara avaliar a real situação no estado do Maranhão.

The objective of this paper is to evaluate the epidemiologic profile of pregnant women submitted to the classification forthe virus HTLV-1 and 2, assisted during prenatal care at three public services. This cross sectional study was conductedbetween 02/11/2008 and 12/03/2008 with 2044 pregnant women in three different public prenatal units in São Luis(MA). The sample was calculated in 2041, test power 95%, absolute mistake of 5%, Stata 9.0 program was used, ChiSquare test was taken, and p<0,05 (95%) was accepted as the limit for significance. The average age was 25 years old;35.12% are single with partners; 41.10% have light brown complexion; 57.19% have finished high school; 54.21% startedsexual activity between 15 and 18 years old; 35.08% have never used condom during sexual intercourse; 54.99%are primiparous women; 15.22% were breastfed by their mothers and other people; 42.51% have already had children,out of these; 31.9% have already practiced crossed breastfeeding; 1.2% have already used illegal drugs. It is concludedthat the pregnant women presented characteristics that justify the classification of virus HTLV-1 and 2 during the prenatalcare, however more studies are necessary for the evaluation of the real situation in the State of Maranhão.

El objetivo de este estudio es evaluar el perfil epidemiológico de las gestantes sometidas a la detección del virusHTLV 1 y 2, en la atención prenatal de tres unidades de salud pública. Estudio transversal realizado entre 11/02 y 03/12/2008, con 2.044 gestantes en tres unidades de salud pública de prenatal, en São Luis (MA). La muestra secalculó en 2.041, poder de test 95%, de error absoluto 5%, Se utilizó el programa Stata 9.0, realizado test chi cuadradoy aceptado p <0.05 (95%) como umbral de significación. Los resultados informan que la edad media fue de 25años; 35,12% son solteras con compañeros; 41,10% de color de piel marrón; 57,19% con enseñanza media completa;54,21% iniciaron la actividad sexual entre los 15 y los 18 años; 35,08% nunca utilizaron preservativos durante lasrelaciones sexuales; 54,99% son de primer embarazo; 15,22% fueron amamantadas por las madres u otras; 42,51%ya tuvieron hijos y de estas el 31,9% ya practicaron alimentación cruzada; 1,2% ya consumieron drogas ilícitas.Se concluye que las gestantes presentan características que justifican la detección del virus del HTLV 1 y 2 duranteel período prenatal, entretanto, se hacen necesarios estudios adicionales para evaluar la situación real en el estadode Maranhão.
Descritores: Cuidado Pré-Natal
Perfil de Saúde
Vírus Linfotrópico T Tipo 1 Humano
Vírus Linfotrópico T Tipo 2 Humano
Triagem
-Programas de Rastreamento
Saúde Materno-Infantil
Enfermagem Materno-Infantil
Limites: Humanos
Feminino
Gravidez
Responsável: BR21.2 - BVS Enfermería


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Id: biblio-1137034
Autor: Souza, Luzinete Santos; Sampaio, Daniela Carneiro; Rivemales, Maria da Conceição Costa; Oliveira, Lavinya Lima Cordeiro; Jesus, Ana Paula Santos de; Lima, Claudia Feio da Maia; Félix, Nuno Damácio de Carvalho.
Título: Diagnósticos de enfermagem em pessoas soropositivas pelo vírus linfotrópico t humano / Diagnósticos de enfermería en personas seropositivas por virus linfotrópicos t humanos / Nursing diagnoses in seropositive people by human t lymphotropic virus
Fonte: Rev. baiana enferm;34:e37991, 2020. tab.
Idioma: pt.
Resumo: Objetivo identificar diagnósticos de enfermagem em pessoas sintomáticas e soropositivas para o vírus linfotrópico T humano. Método pesquisa documental, realizada nos prontuários de pessoas soropositivas para o vírus linfotrópico T humano, cadastradas no Centro de Testagem e Aconselhamento de um município do interior do estado da Bahia. A análise dos registros foi realizada com base na Taxonomia II da NANDA-I 2018-2020. Resultados foram identificados 13 diagnósticos de enfermagem distribuídos em 5 domínios: Conforto; Eliminação e Troca; Atividade e repouso; Autopercepção; Enfrentamento/Tolerância ao estresse. Os diagnósticos mais prevalentes foram: Dor crônica, em 21 (100%) dos soropositivos, Incontinência urinária em nove (43%), e Deambulação prejudicada em 8 (38%). Conclusão a identificação dos diagnósticos em pessoas sintomáticas e soropositivas para o vírus linfotrópico T humano fornece subsídios para a construção de planos de cuidados de enfermagem específicos.

Objetivo identificar los diagnósticos de enfermería en personas sintomáticas y seropositivas para el virus linfotrópico T humano. Método investigación documental, realizada en los registros médicos de personas seropositivas para el virus linfotrópico T humano, registradas en el Centro de Pruebas y Asesoramiento de un municipio en el interior del estado de Bahía. El análisis de los registros se realizó sobre la base de la Taxonomía II de NANDA-I 2018-2020. Resultados se identificaron 13 diagnósticos de enfermería, distribuidos en cinco dominios: Confort; Eliminación e Intercambio; Actividad y descanso; Auto-percepción; Afrontamiento/tolerancia al estrés. Los diagnósticos más frecuentes fueron: Dolor crónico, en 21 (100%) de individuos seropositivos, incontinencia urinaria en nueve (43%) y deterioro de la ambulación en 8 (38%). Conclusión la identificación de diagnósticos en personas sintomáticas y seropositivas para el virus linfotrópico T humano proporciona apoyo para la construcción de planes específicos de cuidados de enfermería.

Objective to identify nursing diagnoses in symptomatic and seropositive people for the human T lymphotropic virus. Method documentary research, carried out with the medical records of people seropositive for the human T lymphotropic virus, registered at the Testing and Counseling Center of a municipality in inland Bahia state. The analysis of the records was performed based on Taxonomy II of NANDA-I 2018-2020. Results 13 nursing diagnoses were identified, distributed in five domains: Comfort; Elimination and Exchange; Activity and rest; Self-perception; Coping/Stress Tolerance. The most prevalent diagnoses were: Chronic Pain, in 21 (100%) of seropositive individuals, urinary incontinence in nine (43%), and Impaired ambulation in 8 (38%). Conclusion the identification of diagnoses in symptomatic and seropositive people for the human T lymphotropic virus provides support for the construction of specific nursing care plans.
Descritores: Diagnóstico de Enfermagem
Infecções por HTLV-I
Cuidados de Enfermagem/métodos
Processo de Enfermagem
-Vírus Linfotrópico T Tipo 1 Humano
Vírus Linfotrópico T Tipo 2 Humano
Limites: Humanos
Responsável: BR21.2 - BVS Enfermería


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Lemos, Marcílio Figueiredo
Moreira, Regina Celia
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Id: biblio-1128443
Autor: Campos, Karoline Rodrigues; Alves, Fabiana Aparecida; Lemos, Marcílio Figueiredo; Moreira, Regina Célia; Marcusso, Rosa Maria Nascimento; Caterino-de-Araujo, Adele.
Título: The reasons to include the serology of human T-lymphotropic virus types 1 and 2 (HTLV-1 and HTLV-2) in the clinical follow-up of patients with viral hepatitis B and C in Brazil
Fonte: PLoS Negl Trop Dis;14(5):e0008245, May 26, 2020. tab, graf.
Idioma: en.
Resumo: The human T-lymphotropic viruses type 1 (HTLV-1) and type 2 (HTLV-2) are endemic and neglected in Brazil, and although these viruses were considered to have prognostic value when associated with HIV, e.g., HTLV-1 has been associated with fast progression and death to AIDS and more cases of lymphoma and neurological disorders, and HTLV-2 has been associated with slow progression to AIDS, their impact on hepatitis B virus (HBV) and hepatitis C virus (HCV) outcomes remain unknown. The present study analyzed the influence of HTLV-1 and HTLV-2 coinfection on HBV and HCV virological outcomes (viral load levels and virus clearance during follow-up) in serum samples from 1,910 patients with hepatitis B and 1,315 patients with hepatitis C from São Paulo, southeast Brazil. The results obtained confirmed the positive impact of HTLV-2 on HBV and HCV virological outcomes (lower HBV and HCV viral loads and increased HCV clearance when compared with HCV-monoinfected patients) and the opposite effect of HTLV-1; these findings are similar to the effects observed in HIV/AIDS patients. Taking into consideration these results and the differential impact of HTLV-1 and HTLV-2 in viral hepatitis B and C, we suggest including the serology of HTLV-1/2 in the follow-up of such patients in Brazil. (AU)
Descritores: Pacientes
Sorologia
Vírus Linfotrópico T Tipo 1 Humano
Vírus Linfotrópico T Tipo 2 Humano
Hepatite Viral Humana
Limites: Humanos
Masculino
Feminino
Responsável: BR91.2 - Centro de Documentação


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Id: biblio-1128430
Autor: Campos, Karoline Rodrigues; Caterino-de-Araujo, Adele.
Título: Provirus Mutations of Human T-Lymphotropic Virus 1 and 2 (HTLV-1 and HTLV-2) in HIV-1-Coinfected Individuals
Fonte: MSphere;5(5):e00923-20, Sept.-Oct. 2020. tab, graf.
Idioma: en.
Resumo: Provirus mutations of human T-lymphotropic virus 1 (HTLV-1), mostly the lack of the 5= long terminal repeat (LTR) genomic region, have been described and associated with severe adult T cell leukemia/lymphoma (ATLL), non-sense point mutations with low proviral load, and Western blotting indeterminate results. Until now, no information concerning provirus mutations of HTLV-2 and its consequences, as well as those of HTLV-1/2 in HIV-coinfected individuals, had been described. Therefore, we searched for these mutations in provirus samples of 44 HIV/HTLV-1- and 25 HIV/HTLV-2-coinfected individuals. Using protocols well established for amplification and sequencing of segments of the LTR, env, and tax regions, we searched for defective type 1 particles that retain LTRs and lack internal sequences and type 2 particles that lack the 5=LTR region. In addition, using as references the prototypes ATK (HTLV-1) and Mo (HTLV-2), we searched for point mutations in the LTR and synonyms and nonsynonymous mutations and non-sense mutations in env and tax regions. Defective HTLV-1 and HTLV-2 provirus type 1 or 2 was detected in 31.8% of HIV/HTLV-1- and 32.0% of HIV/HTLV-2-coinfected individuals. Synonymous and nonsynonymous mutations were identified mostly in HTLV-2 and associated with lower levels of specific antibodies. No non-sense mutations that resulted in premature termination of Env and Tax proteins were detected. On the contrary, mutation in the stop codon of Tax2a produced a long protein characteristic of the HTLV-2c subtype. The clinical significance of these mutations in coinfected individuals remains to be defined, but they confirmed the lower sensitivity of serological and molecular diagnostic tests in HIV/HTLV-1/2 coinfections. IMPORTANCE HTLV-1 and HTLV-2 are endemic to Brazil, and they have different effects in HIV/AIDS disease progression. HIV/HTLV-1 has been described as accelerating the progression to AIDS and death, while HIV/HTLV-2 slows the progression to AIDS. Provirus mutations of HTLV-1 were implicated in severe leukemia development and in problems in the diagnosis of HTLV-1; in contrast, provirus mutations of HTLV-2 had not been confirmed and associated with problems in HTLV-2 diagnosis or disease outcome. Nevertheless, data obtained here allowed us to recognize and understand the false-negative results in serologic and molecular tests applied for HTLV-1 and HTLV-2 diagnosis. Defective proviruses, as well as synonymous and nonsynonymous mutations, were associated with the diagnosis deficiencies. Additionally, since HIV-1 and HTLV-1 infect the same cells (CD4 positive), the production of HIV-1 pseudotypes with HTLV-1 envelope glycoprotein during HIV/HTLV-1 coinfection cannot be excluded. Defective provirus of HTLV-2 and Tax2c is speculated to influence progression to AIDS. (AU)
Descritores: Vírus Linfotrópico T Tipo 1 Humano
Vírus Linfotrópico T Tipo 2 Humano
Síndrome de Imunodeficiência Adquirida
HIV
Provírus
Coinfecção
Mutação
Responsável: BR91.2 - Centro de Documentação


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Id: biblio-1121386
Autor: Brito, Vanessa da Silva; Santos, Fred Luciano Neves; Golçalves, Noilson Lazaro Souza; Araujo, Thessika Hialla Almeida; Nascimento, Davi Silva Vale; Pereira, Felicidade Mota; Boa-Sorte, Ney Cristian Amaral; Grassi, Maria Fernanda Rios; Caterino de Araujo, Adele; Castro, Bernardo Galvão.
Título: Performance of commercially available serological screening Tests for human T-Cell lymphotropic virus infection in Brazil
Fonte: Journal of Clinical Microbiology;56(12), 2018. graf.
Idioma: en.
Resumo: ABSTRACT Serological screening for human T-cell lymphotropic virus type 1 (HTLV-1) is usually performed using enzyme-linked immunosorbent assay (ELISA), particle agglutination, or chemiluminescence assay kits. Due to an antigen matrix improvement entailing the use of new HTLV antigens and changes in the format of HTLV screening tests, as well as newly introduced chemiluminescence assays (CLIAs), a systematic evaluation of the accuracy of currently available commercial tests is warranted. We aimed to assess the performance of commercially available screening tests for HTLV infection diagnosis. A diagnostic accuracy study was conducted on a panel of 397 plasma samples: 200 HTLV-negative plasma samples, 170 HTLV-positive plasma samples, and 27 plasma samples indeterminate by Western blotting (WB). WB-indeterminate samples (i.e., those yielding no specific bands for HTLV-1 and/or HTLV-2) were assessed by PCR, and the results were used to compare agreement among the commercially available ELISA screening tests. For performance analysis, WB-indeterminate samples were excluded, resulting in a final study panel of 370 samples. Three ELISA kits (Murex HTLV-1/2 [Murex], anti-HTLV-1/2 SYM Solution [SYM Solution], and Gold ELISA HTLV-1/2 [Gold ELISA]) and one CLIA kit (Architect rHTLV- 1/2) were evaluated. All screening tests demonstrated 100% sensitivity. Concerning the HTLV-negative samples, the SYM Solution and Gold ELISA kits had specificity values of 99.5%, while the Architect rHTLV-1/2 test presented 98.1% specificity, followed by Murex, which had a specificity of 92.0%. Regarding the 27 samples with WB-indeterminate results, after PCR confirmation, all ELISA kits showed 100% sensitivity but low specificity. Accuracy findings were corroborated by the use of Cohen's kappa value, which evidenced slight and fair agreement between PCR analysis and ELISAs for HTLV infection diagnosis. Based on the data, we believe that all evaluated tests can be safely used for HTLV infection screening.
Descritores: Vírus Linfotrópico T Tipo 1 Humano/imunologia
Vírus Linfotrópico T Tipo 2 Humano/imunologia
Infecções por Deltaretrovirus/diagnóstico por imagem
Programas de Rastreamento
-Kit de Reagentes para Diagnóstico
Ensaio de Imunoadsorção Enzimática
Reação em Cadeia da Polimerase
Sensibilidade e Especificidade
Limites: Humanos
Responsável: BR91.2 - Centro de Documentação


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Id: biblio-1121310
Autor: Campos, Karoline Rodrigues; Gonçalves, Maria Gisele; Caterino de Araujo, Adele.
Título: Short communication: failures in detecting HTLV-1 and HTLV-2 in patients infected with HIV-1
Fonte: AIDS Research and retoviruses;33(4):382-385, 2017. graf.
Idioma: pt.
Resumo: Changes in retrovirus acquisition/transmission behaviors have been reported in Brazil, with a concerning increase in HIV-1-infected individuals aged 15-39 years. In São Paulo, HIV-1/HTLV-1 and HIV-1/HTLV-2 coinfections have been associated with intravenous drug use and failure to detect HTLV-1/2 (human T cell lymphotropic virus types 1 and 2) with immunosuppression and the use of highly active antiretroviral therapy (HAART). Negative results for HTLV serologic [western blotting (WB)] and molecular [real-time PCR pol (qPCR)] confirmatory assays have been reported, whereas the best sensitivity has been found for INNO-LIA (LIA). In this study, we expand our previous data by analyzing a group of young patients (n = 1,383; median age 35.6 years) who recently acquired HIV by sexual contact, the majority of whom were HAART naïve, and comparing the performances of four HTLV confirmatory assays LIA, WB, qPCR, and PCR-RFLP (tax). We confirmed HTLV infection in 58 (4.2%) blood samples 29 HTLV-1, 24 HTLV-2, 1 HTLV-1+HTLV-2, and 4 HTLV. LIA, WB, qPCR, and PCR-RFLP sensitivities were 94.8%, 82.8%, 79.2%, and 74.5%, respectively. Associations of HTLV infection with female gender (OR = 2.28, 1.31-4.00) and age >40 years (p < .0001) were detected. The results confirm the low sensitivities of molecular assays and the best performance of LIA in detecting HTLV-1/2 in such patients. We hypothesize that the negative PCR results are due to the presence of defective provirus and/or low proviral load circulating in such patients, with inconclusive WB coinciding with the seroconversion period. Corroborating the associations obtained, repeated exposure is required for HTLV sexual transmission/acquisition, which is more efficient from male to female
Descritores: Vírus Linfotrópico T Tipo 1 Humano/imunologia
Infecções por HTLV-I/diagnóstico
Vírus Linfotrópico T Tipo 2 Humano/imunologia
Infecções por HTLV-II/diagnóstico
HIV-1
Infecções Oportunistas Relacionadas com a AIDS/diagnóstico
-Western Blotting
Sensibilidade e Especificidade
Limites: Humanos
Masculino
Feminino
Responsável: BR91.2 - Centro de Documentação


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Id: biblio-1057394
Autor: Borda, Marcelo A; Svibel, Graciela R; Biglione, Mirna M; Berini, Carolina A.
Título: Hallazgo del virus linfotrópico T humano 1 (HTLV-1) subtipo Cosmopolita subgrupo Transcontinental (Aa) y del HTLV-2 subtipo b en donantes de sangre de Corrientes / Detection of Human T lymphotropic virus 1 (HTLV-1) Cosmopolitan subtype Transcontinental subgroup (Aa) and HTLV-2 subtype b in blood donors of Corrientes
Fonte: Rev. argent. microbiol;51(4):307-315, dic. 2019. graf.
Idioma: es.
Resumo: Resumen Se realizó un estudio epidemiológico molecular en una población de 9.422 donantes de sangre de la provincia de Corrientes (noreste de Argentina), con el fin de determinar la prevalencia del virus linfotrópico T del humano tipos 1 y 2 (human T-cell lymphotropic virus: HTLV-1/2), de identificar filogenéticamente a los subtipos/subgrupos de HTLV-1 y 2 encontrados y de realizar el análisis de mutaciones. Sobre la base de los resultados obtenidos, se demostró que tanto el HTLV-1 como el HTLV-2 se encuentran circulando en una población de bajo riesgo de Corrientes, si bien con una prevalencia similar a las de áreas no endémicas. Los estudios filogenéticos identificaron al subtipo Cosmopolita subgrupo Transcontinental (Aa) del HTLV-1 y al subtipo b del HTLV-2. Los donantes infectados no manifestaron antecedentes de riesgo tales como transfusiones, uso de drogas inyectables ni parejas sexuales de riesgo o seropositivas para HTLV-1/2. Estos resultados indican que estos virus fueron transmitidos de madre a hijo, posiblemente de generación en generación, y que estas cepas fueron introducidas en la población caucásica de esta región a partir de ascendientes originarios de áreas endémicas del país o por contacto producido tiempo atrás con individuos infectados de otros países. Nuestros resultados demuestran por primera vez la presencia de HTLV-1 y HTLV-2 en la provincia de Corrientes. Y si bien se puede considerar a esta provincia como área no endémica, se destaca la necesidad de incluir a estos retrovirus en un programa nacional de salud pública, con el fin de contar con profesionales capacitados para realizar su diagnóstico y brindar la información necesaria en relación con la atención primaria y el seguimiento de los pacientes.

Abstract A molecular epidemiological study was conducted in a population of 9422 blood donors in the province of Corrientes, Northeastern Argentina, to determine the prevalence of Human T-cell lymphotropic virus types 1 and 2 (HTLV-1/2), the phylogenetic identification of HTLV-1 and 2 subtypes/subgroups and perform a mutation analysis. Based on the results obtained, it was shown that both HTLV-1 and HTLV-2 are circulating in a low-risk population of Corrientes, although with a similar prevalence to that of non-endemic areas. Phylogenetic studies identified the HTLV-1 Cosmopolitan subtype Transcontinental subgroup (Aa), and the HTLV-2 subtype b. Infected donors reported neither a history of risk factors such as transfusions, intravenous drug use, nor risky or HTLV-1/2 seropositive sexual partners. These results suggest that these viruses were transmitted from mother to child, possibly from generation to generation, and that these strains were introduced into the Caucasian population of this region from ancestors originating from endemic areas of the country either from or through contact with individuals from other countries years ago. Our results demonstrate for the first time the presence of HTLV-1 and HTLV-2 in the province of Corrientes. Moreover, although the province can be considered a non-endemic area, the need to include these retroviruses in a national Public Health program is highlighted, in order to have qualified professionals duly trained to make their diagnosis and provide the necessary information in relation to primary care and patient follow-up.
Descritores: Vírus Linfotrópico T Tipo 1 Humano/genética
Vírus Linfotrópico T Tipo 2 Humano/genética
-Argentina/epidemiologia
Doadores de Sangue
Análise Mutacional de DNA/métodos
Estudos Epidemiológicos
Prevalência
Limites: Humanos
Masculino
Feminino
Tipo de Publ: Ensaio Clínico
Estudo de Avaliação
Responsável: AR635.1 - FCVyS - Servicio de Información y Documentación



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