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Id: lil-444842
Autor: Torres, M. W; Corrêa, R. L; Schrago, C. G.
Título: Analysis of differential selective forces acting on the coat protein (P3) of the plant virus family Luteoviridae
Fonte: Genet. mol. res. (Online);4(4):790-802, 2005. tab, graf, ilus.
Idioma: en.
Resumo: The coat protein (CP) of the family Luteoviridae is directly associated with the success of infection. It participates in various steps of the virus life cycle, such as virion assembly, stability, systemic infection, and transmission. Despite its importance, extensive studies on the molecular evolution of this protein are lacking. In the present study, we investigate the action of differential selective forces on the CP coding region using maximum likelihood methods. We found that the protein is subjected to heterogeneous selective pressures and some sites may be evolving near neutrality. Based on the proposed 3-D model of the CP S-domain, we showed that nearly neutral sites are predominantly located in the region of the protein that faces the interior of the capsid, in close contact with the viral RNA, while highly conserved sites are mainly part of beta-strands, in the protein's major framework.
Descritores: Genoma Viral
Imageamento Tridimensional/métodos
Modelos Genéticos
Proteínas do Capsídeo/genética
Interpretação de Imagem Assistida por Computador
Responsável: BR1.1 - BIREME

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Id: lil-365981
Autor: Ehrenfeld, Nicole; Romano, Eduardo; Serrano, Carolina; Arce-Johnson, Patricio.
Título: Replicase mediated resistance against Potato Leafroll Virus in potato Desiree plants
Fonte: Biol. Res;37(1):71-82, 2004. ilus, tab, graf.
Idioma: en.
Resumo: Potato leafroll virus (PLRV) is a major menace for the potato production all over the world. PLRV is transmitted by aphids, and until now, the only strategy available to control this pest has been to use large amounts of insecticides. Transgenic approaches involving the expression of viral replicases are being developed to provide protection for plants against viral diseases. The purpose of this study was to compare the protection afforded by the differential expression of PLRV replicate transgene in potato plants cv. Desirée, Plants were genetically modified to express the complete sense PLRV replicase gene. Two constructions were used, one containing the constitutive 35SCaMV promoter and the other the phloem-specific RolA promoter from Agrobacterium rhizogenes. Transgenic plants were infected with PLRV in vitro, using infested aphids. In plants in which 35SCaMV controlled the expression of the PLRV replicase gene, signs of infection were initially detected, although most plants later developed a recovery phenotype showing undetectable virus levels 40 days after infection.
Descritores: Luteovirus
Doenças das Plantas
Plantas Geneticamente Modificadas
Solanum tuberosum
-Hibridização In Situ
Doenças das Plantas
Reação em Cadeia da Polimerase
RNA Replicase
Solanum tuberosum
Transformação Genética
Tipo de Publ: Estudo Comparativo
Responsável: BR1.1 - BIREME

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