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Texto completo SciELO Chile
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Id: lil-591921
Autor: Milosevic, Snezana; Subotic, Angelina; Bulajic, Aleksandra; Djekic, Ivana; Jevremovic, Sladjana; Vucurovic, Ana; Krstic, Branka.
Título: Elimination of TSWV from impatiens hawkerii Bull. and regeneration of virus-free plant
Fonte: Electron. j. biotechnol;14(1):3-4, Jan. 2011. ilus, tab.
Idioma: en.
Projeto: Serbian Ministry of Science and Technological Development.
Resumo: The possibility for obtaining virus free plants from Impatiens hawkerii Bull. shoots infected with Tomato spotted wilt virus (TSWV) through meristem-tip culture was examined. TSWV presence in I. hawkerii plants was detected by DAS-ELISA and RT-PCR and identification of the virus was confirmed by sequencing one of the chosen isolate (GenBank Accesion CQ132190). Meristem-tip explants (0.3-1.5 mm) from virus-infected shoots are cultured on MS media supplemented with different concentrations of the cytokinins, CPPU or TDZ (0.01-1.0 uM), respectively. Using this system, a large number of in vitro shoots could be produced from a single explant. Also, cytokinins showed a stimulatory effect on the length, fresh and dry weights of the newly formed shoots. Plant pigments content in I. hawkerii shoots increased significantly in the presence of cytokinins. Rooting of shoots was spontaneous on the same media. Rooted plantlets were transferred to soil where 97 percent successfully acclimatized. By DAS-ELISA and RT-PCR, 80 percent of the in vitro plantlets were shown to be a virus-free. Considering these, the present protocol seems to be an efficient method for in vitro generation of virus-free I. hawkerii plantlets by meristem tip cultures.
Descritores: Organismos Livres de Patógenos Específicos/fisiologia
Tospovirus/fisiologia
-Meristema/fisiologia
Preparações de Plantas
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Brasil
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Id: lil-297652
Autor: Resende, Luciane V; Maluf, Wilson R; Figueira, Antônia dos R; Resende, Juliano T. V.
Título: Correlations between symptoms and DAS - ELISA values in two sources of resistance against tomato spotted wilt virus
Fonte: Braz. j. microbiol;31(2):135-9, Apr.-Jun. 2000. tab.
Idioma: en.
Resumo: The commercial tomato, "Lycopersicon esculentum", cultivars Ângela Gigante I-5100 and Santa Clara (tsopovirus susceptible), the Stevens cultivar, lines and experimental hybrids (tospovirus resistant), were used : a) to evaluate two sources of resistance to tospoviruses in tomato (one from "L. esculentum"/Rey de Los Tempranos, and another one from "L. peruvianum"/Stevens); and b) to verify statistically significant correlation between virus concentration determined through DAS-ELISA and symptomatology. In the Ângela GiganteI-5100 and the Santa Clara cultivars, the correlation coefficient was highly significant and the symptom average level was high, showing that in susceptible cultivars the symptom evolution is related to the virus multiplication in the plant. As for the TOM 547 and TOM 556 lines (background of Ângela Gigante I-5100 and Santa Clara, respectively), there was no statistical correlation between the symptoms and the viral particule concentration. Plants with light virus symptoms showed high absorbance values. Consequently we may assume that the resistance with 'Rey de Los Tempranos' background may be of the tolerant kind because the high virus concentration found does not necessarily relate to a high level symptoms. In the lines BPX320E 3902-01, BPX320E 3905 and BPX320F 7902 (both with Santa Clara background) only few plants showed symptoms, which can be explained by incomplete penetrance of Sw-5 gene. In the case of non-symptomatic plants, the diagnosis using DAS-ELISA revealed negative results. That outcome indicates that in some materials, where resistance is obtained from "L. peruvianum", the virus multiplication in the tissues seemed not be present.
Descritores: Técnicas In Vitro
Tospovirus/genética
Viroses/genética
Viroses/fisiopatologia
-Ensaio de Imunoadsorção Enzimática
Testes Sorológicos/métodos
Tipo de Publ: Relatório Técnico
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica



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