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Id: biblio-1100481
Autor: Sapia, Elizabeth Y; Maroni, Camila; Groisman, Carolina; Kromer, Hilen; Lihue Rojo, Gabriel; Dastugue, Mónica; Valinotto, Laura.
Título: Genotipificación del virus de la enfermedad mano-pie-boca atípica en un hospital pediátrico de la Ciudad de Buenos Aires, Argentina / Atypical hand-foot-mouth disease virus genotyping in a pediatric hospital in Buenos Aires city, Argentin
Fonte: Arch. argent. pediatr;118(2):e199-e203, abr. 2020. ilus.
Idioma: es.
Resumo: La enfermedad mano-pie-boca (EMPB) típica es exantemática, con sintomatología clásica de fiebre, exantema papulovesicular en las manos y los pies, asociada o no a herpangina. Es causada, principalmente, por enterovirus 71 y virus Coxsackie A16, miembros del género Enterovirus. En los últimos años, se han descrito brotes mundiales de EMPB con manifestaciones atípicas causadas, sobre todo, por el virus Coxsackie A6. La EMPB atípica se considera emergente con características clínicas y epidemiológicas peculiares: la afección de adultos, el predominio en invierno y un amplio espectro de manifestaciones clínicas en la extensión y la distribución de las lesiones. Las características morfológicas de las lesiones son muy variables: pueden simular varicela, impétigo o vasculitis.Se describe el caso de un niño de 4 años con EMPB atípica. Se detalla su forma de presentación, evolución clínica, metodología diagnóstica y terapéutica empleada.

Typical hand-foot-mouth disease (HFMD) is an exanthematous viral disease with a classic symptomatology of fever, papulovesicular rash on the hands and feet with or without herpangina. It is usually caused by enterovirus 71 and Coxsackievirus A16, members of the genus Enterovirus. Recently, worldwide outbreaks of HFMD with atypical manifestations caused by Coxsackievirus A6 have been described. Atypical HFMD is considered an emerging disease due to its peculiar clinical and epidemiological characteristics: it affects adults, has a wide spectrum of clinical manifestations in the extension and distribution of the lesions and occurs in winter. The morphological characteristics of the lesions are very variable and can be misdiagnosed as chickenpox, impetigo or vasculitis. Here we describe the symptoms, clinical evolution, diagnostic methodology and treatment employed on a 4-year-old male patient with atypical HFMD.
Descritores: Enterovirus Humano A/classificação
Doença de Mão, Pé e Boca/diagnóstico
-Infecções por Coxsackievirus/epidemiologia
Diagnóstico Diferencial
Genótipo
Doença de Mão, Pé e Boca/terapia
Limites: Humanos
Masculino
Pré-Escolar
Tipo de Publ: Relatos de Casos
Responsável: AR94.1 - Centro de Información Pediatrica


  2 / 7 LILACS  
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Id: biblio-869092
Autor: Acosta Cabello, Alina; Russomando, Graciela; Espínola, Emilio E.
Título: Análisis filogenético y de presión evolutiva de secuencias nucleotídicas del gen VP4 de especies de enterovirus humanos / Phylogenetic and evolutive pressure analyses of nucleotide sequences of VP4 gene of human enterovirus species
Fonte: Mem. Inst. Invest. Cienc. Salud (Impr.);14(2):17-24, ago. 2016. tab, ilus.
Idioma: es.
Resumo: El género Enterovirus es un grupo viral que afecta a un amplio rango de hospederos, entre ellos los humanos (especies A, B, C, y D), causan enfermedades respiratorias, gastrointestinales, neurológicas, y otras, y son altamente contagiosos. Los síntomas pueden ser leves o graves. El objetivo del trabajo fue analizar la variación nucleotídica, filogenética y de presión evolutiva de secuencias nucleotídicas del gen VP4 de las cuatro especies que afectan a los humanos. Se emplearon 92 secuencias nucleotídicas disponibles en la base de datos GenBank; éstas se editaron con el software BioEdit y se alinearon con Clustal W; las relaciones filogenéticas se determinaron con MEGA6, y las presiones evolutivas con los algoritmos SNAP y SLAC. Se encontró que la identidad nucleotídica mínima intra-especie fue de 43,2% (especie B) a 72,6% (especie D). Los genotipos más variables por especie fueron EV-71 (A), Echovirus 2 (B), EV-118 (C), y EV-94 (D). El análisis de presión evolutiva mostró que el gen VP4 en las cuatro especies evoluciona bajo presión selectiva negativa. Esto indicaría que la alta tasa mutacional y eventos de recombinación no tienen un rol significativo en la evolución de este gen, debido probablemente a la localización interna de la proteína VP4.

The Enterovirus genus is a viral group that affects a wide host range, including humans (species A, B, C and D), cause respiratory, gastrointestinal, and neurologic disease, amongothers, and are highly contagious. The symptoms range from mild to severe. The objectiveof this study was to perform a nucleotidic variation, phylogenetic and selective pressureanalyses of the VP4 gene from the four enterovirus species that affect humans. Ninety-twonucleotide sequences (available in the GenBank database) were employed; they were edited with Bio Edit software and aligned with Clustal W; the phylogenetic relationships weredetermined with MEGA6, and the evolutive pressures with SNAP and SLAC algorithms. Itwas found an intra-species nucleotide identity of at least 43,2% (species B) to 72,6% (species D). The more variable genotypes by species were EV-71 (A), Echovirus 2 (B), EV-118 (C), and EV-94 (D). The selective pressure analysis showed that VP4 gene of the fourspecies evolves by negative pressure. This would indicate that the high mutation rate andrecombination events do not have a significant role in the evolution of this gene, probablydue to the internal localization of the VP4 protein.
Descritores: Enterovirus Humano A
Infecções por Enterovirus
Limites: Humanos
Responsável: PY3.1 - Biblioteca


  3 / 7 LILACS  
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Id: biblio-888930
Autor: Zhang, Y; Han, H; Sun, L; Qiu, H; Lin, H; Yu, L; Zhu, W; Qi, J; Yang, R; Pang, Y; Wang, X; Lu, G; Yang, Y.
Título: Antiviral activity of shikonin ester derivative PMM-034 against enterovirus 71 in vitro
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;50(10):e6586, 2017. tab, graf.
Idioma: en.
Projeto: Changjiang Scholars and Innovative Research Team in University; . National Natural Science Foundation of China; . Fundamental Research Funds for the Central Universities.
Resumo: Human enterovirus 71 (EV71) is the major causative agent of hand, foot, and mouth disease (HFMD), particularly in infants and children below 4 years of age. Shikonin is a bioactive compound with anti-inflammatory, antiviral, and antibacterial activities derived from the roots of the Chinese medicinal herb Lithospermum erythrorhizon. This study aimed to examine the antiviral activity of PMM-034, a shikonin ester derivative, against EV71 in rhabdomyosarcoma (RD) cells. Cytotoxicity of PMM-034 on RD cells was determined using WST-1 assay. Dose- and time-dependent effects of PMM-034 on EV71 replication in RD cells were determined using plaque reduction assay. mRNA expression levels of EV71/VP1 and pro-inflammatory cytokines (IL-1β, IL-6, IL-8, and TNF-α) were determined by real-time RT-PCR, and EV71/VP1 and phospho-p65 protein expressions were determined by western blot analysis. PMM-034 exhibited only weak cytotoxicity against RD cells. However, PMM-034 exhibited significant antiviral activity against EV71 in RD cells with 50% inhibitory concentration of 2.31 μg/mL. The VP1 mRNA and protein levels were significantly reduced in cells treated with PMM-034. Furthermore, relative mRNA expression levels of IL-1β, IL-6, IL-8, and TNF-α significantly decreased in the cells treated with PMM-034, while the phospho-p65 protein expression was also significantly lower in the treated cells. These results indicated that PMM-034 suppressed the expressions of pro-inflammatory cytokines in RD cells, exhibiting antiviral activity against EV71, as evidenced by the reduced VP1 mRNA and protein levels in PMM-034-treated cells. Thus, PMM-034 is a promising candidate for further development as an EV71 inhibitor.
Descritores: Antivirais/farmacologia
Enterovirus Humano A/efeitos dos fármacos
Naftoquinonas/farmacologia
Rabdomiossarcoma/virologia
-Western Blotting
Linhagem Celular Tumoral
Citocinas/análise
Relação Dose-Resposta a Droga
Reação em Cadeia da Polimerase em Tempo Real
Testes de Toxicidade
Ensaio de Placa Viral
Replicação Viral/efeitos dos fármacos
Limites: Humanos
Responsável: BR1.1 - BIREME


  4 / 7 LILACS  
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Texto completo SciELO Saúde Pública
Szwarcwald, Célia Landmann
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Id: lil-776698
Autor: Malta, Deborah Carvalho; Stopa, Sheila Rizzato; Szwarcwald, Celia Landmann; Gomes, Nayara Lopes; Silva Júnior, Jarbas Barbosa; Reis, Ademar Arthur Chioro dos.
Título: A vigilância e o monitoramento das principais doenças crônicas não transmissíveis no Brasil - Pesquisa Nacional de Saúde, 2013 / Surveillance and monitoring of major chronic diseases in Brazil - National Health Survey, 2013
Fonte: Rev. bras. epidemiol;18(supl.2):3-16, Out.-Dez. 2015. tab.
Idioma: en.
Resumo: RESUMO: Objetivo: Descrever as principais doenças crônicas não transmissíveis (DCNT) no país segundo as informações coletadas em indivíduos de 18 anos ou mais de idade. Métodos: Foram utilizados dados da Pesquisa Nacional de Saúde (PNS), 2013, estudo transversal de base populacional. As proporções de cada DCNT foram calculadas e apresentadas segundo sexo, com intervalo de confiança de 95% (IC95%), com os valores absolutos. Resultados: Do total de entrevistados, 45,1% referiram ter pelo menos uma DCNT. A Região com maior prevalência de DCNT foi a Sul (52,1%). A hipertensão arterial apresentou a maior prevalência dentre as DCNT, com 21,4%, seguida por problema crônico de coluna (18,5%), depressão (7,6%), artrite (6,4%) e diabetes (6,2%). O grau de limitação intenso/muito intenso apresentou maiores prevalências para outra doença mental (37,6%) e acidente vascular cerebral (AVC) (25,5%). Conclusão: A melhoria dos serviços de saúde é indispensável para uma resposta efetiva à dupla carga de adoecimento de países de média e baixa renda.

ABSTRACT: Objective: To describe the major noncommunicable diseases (NCDs) in Brazil, according to the information collected from individuals aged 18 years or older. Methods: Data from the National Health Survey (PNS), 2013, a transversal population-based study, were used. The proportions of each NCD were calculated and presented according to sex, with a 95% confidence interval (95%CI), with the absolute values. Results: Of the total respondents, 45.1% reported presenting at least one NCD. The region with the highest prevalence of NCDs was the South (52.1%). Hypertension showed the highest prevalence among NCDs, with 21.4%, followed by chronic back problem (18.5%), depression (7.6%), arthritis (6.4%), and diabetes (6.2%). The intense/very intense degree of limitation showed a higher prevalence of other mental illnesses (37.6%) and cerebrovascular accident (25.5%). Conclusion: The improvement of health services is essential for an effective response to the double burden of illness in the middle- and low-income countries.
Descritores: Antivirais/farmacologia
/metabolismo
CYCLOOXYGENASE TEMEFOS/metabolismo
Enterovirus Humano A/efeitos dos fármacos
Infecções por Enterovirus/genética
Isoflavonas/farmacologia
Prostaglandinas E/metabolismo
Replicação Viral/efeitos dos fármacos
-/genética
CYCLOOXYGENASE TEMEFOS/genética
Enterovirus Humano A/fisiologia
Infecções por Enterovirus/enzimologia
Infecções por Enterovirus/metabolismo
Infecções por Enterovirus/virologia
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-705289
Autor: Shi, Mei; Zhou, Yaping; Cao, Limin; Ding, Cuijun; Ji, Yun; Jiang, Qinbo; Liu, Xiping; Li, Xiang; Hou, Xueling; Peng, Hongjun; Shi, Weifeng.
Título: Expression of enterovirus 71 capsid protein VP1 in Escherichia coli and its clinical application
Fonte: Braz. j. microbiol;44(4):1215-1222, Oct.-Dec. 2013. ilus, graf, tab.
Idioma: en.
Resumo: The VPl gene of enterovirus 71 (EV71) was synthesized, construct a recombinant plasmid pET15b/VP1 and expressed in E. coli BL21. The recombinant VP1 protein could specifically react with EV71-infected patient sera without the cross-reaction with serum antibodies of coxsackievirus A16 (CA16), A4, A5, B3 and B5 as well as echovirus 6. In acute and convalescent phases, IgM and IgG antibodies of 182 serum samples were detected by ELISA with recombinant VP1 protein as a coated antigen. The results showed that the sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of IgM antibodies in serum samples for the diagnosis of EV71 infection were 90.1, 98.4, 98.8 and 88.7%, respectively; similarly, those of IgG antibodies in serum samples were 82.4, 89.1, 91.5 and 78.1%, respectively. Five of 80 samples (6.25%) from CA16infected patients were detected positive by ELISA with recombinant VP1 protein in which indicated the cross reactions and 0 of 5 samples from patients infected with other enteroviruses including CA4, CA5, CB3, CB5 and echovirus 6. Therefore, the recombinant VP1 protein of EV7l may provide a theoretical reference for establishing an effective antibody screening of IgM for EV71-infected patients with clinically suspected hand, foot, and mouth disease (HFMD).
Descritores: Anticorpos Antivirais/sangue
Proteínas do Capsídeo
Enterovirus Humano A/imunologia
Doença de Mão, Pé e Boca/diagnóstico
-Clonagem Molecular
Proteínas do Capsídeo/genética
Proteínas do Capsídeo/imunologia
Enterovirus Humano A/genética
Ensaio de Imunoadsorção Enzimática/métodos
Escherichia coli/genética
Expressão Gênica
Imunoglobulina G/sangue
Imunoglobulina M/sangue
Valor Preditivo dos Testes
Proteínas Recombinantes
Proteínas Recombinantes/genética
Proteínas Recombinantes/imunologia
Sensibilidade e Especificidade
Testes Sorológicos/métodos
Limites: Pré-Escolar
Feminino
Humanos
Lactente
Masculino
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: lil-683127
Autor: Shi, Weifeng; Hou, Xueling; Li, Xiang; Peng, Hongjun; Shi, Mei; Jiang, Qingbo; Liu, Xiping; Ji, Yun; Yao, Yuhua; He, Caizhen; Lei, Xiangdong.
Título: Differential gene expressions of the MAPK signaling pathway in enterovirus 71-infected rhabdomyosarcoma cells
Fonte: Braz. j. infect. dis;17(4):410-417, July-Aug. 2013. ilus, tab.
Idioma: en.
Resumo: BACKGROUND: Mitogen-activated protein kinase (MAPK) signaling pathway plays an important role in response to viral infection. The aim of this study was to explore the function and mechanism of MAPK signaling pathway in enterovirus 71 (EV71) infection of human rhabdomyosarcoma (RD) cells. METHODS: Apoptosis of RD cells was observed using annexin V-FITC/PI binding assay under a fluorescence microscope. Cellular RNA was extracted and transcribed to cDNA. The expressions of 56 genes of MAPK signaling pathway in EV71-infected RD cells at 8 h and 20 h after infection were analyzed by PCR array. The levels of IL-2, IL-4, IL-10, and TNF-α in the supernatant of RD cells infected with EV71 at different time points were measured by ELISA. RESULTS: The viability of RD cells decreased obviously within 48 h after EV71 infection. Compared with the control group, EV71 infection resulted in the significantly enhanced releases of IL-2, IL-4, IL-10 and TNF-α from infected RD cells (p < 0.05). At 8 h after infection, the expressions of c-Jun, c-Fos, IFN-i, MEKK1, MLK3 and NIK genes in EV71-infected RD cells were up-regulated by 2.08-6.12-fold, whereas other 19 genes (e.g. AKT1, AKT2, E2F1, IKK and NF-κB1) exhibited down-regulation. However, at 20 h after infection, those MAPK signaling molecules including MEKK1, ASK1, MLK2, MLK3, NIK, MEK1, MEK2, MEK4, MEK7, ERK1, JNK1 and JNK2 were up-regulated. In addition, the expressions of AKT2, ELK1, c-Jun, c-Fos, NF-κB p65, PI3K and STAT1 were also increased. CONCLUSION: EV71 infection induces the differential gene expressions of MAPK signaling pathway such as ERK, JNK and PI3K/AKT in RD cells, which may be associated with the secretions of inflammatory cytokines and host cell apoptosis.
Descritores: Enterovirus Humano A/genética
Proteínas Quinases Ativadas por Mitógeno/genética
Rabdomiossarcoma/virologia
-Citocinas/genética
Ensaio de Imunoadsorção Enzimática
Enterovirus Humano A/enzimologia
Enterovirus Humano A/fisiologia
Regulação Neoplásica da Expressão Gênica
Proteínas Quinases Ativadas por Mitógeno/fisiologia
Reação em Cadeia da Polimerase
Rabdomiossarcoma/enzimologia
Rabdomiossarcoma/genética
Transdução de Sinais/genética
Transdução de Sinais/fisiologia
Fatores de Tempo
Células Tumorais Cultivadas
Regulação para Cima
Replicação Viral
Limites: Humanos
Responsável: BR1.1 - BIREME


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Timenetsky, Maria do Carmo S. T
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Id: lil-570735
Autor: Luchs, Adriana; Cilli, Audrey; Russo, Denise H; Costa, Fernanda F; Carmona, Rita de Cássia C; Timenetsky, Maria do Carmo S. T.
Título: Evaluation of enterovirus 71 immune status in São Paulo state, Brazil / Avaliação da situação imunitária contra enterovírus 71 no estado de São Paulo, Brasil
Fonte: Rev. Inst. Med. Trop. Säo Paulo;52(6):339-341, Nov.-Dec. 2010.
Idioma: en.
Resumo: Antibodies to Enterovirus 71 (EV71) were evaluated in São Paulo State during 1999-2005. The titer of neutralizing antibodies against EV71 was determined by microneutralization assay, and a titer of > 1:8 was defined as indicative of protected immunity. Neutralizing antibodies to EV71 were observed in 12.4 percent (55/442) of sera samples, a low protective rate, suggesting that EV71 infection is uncommon in this region, but that there is a relatively high susceptibility to EV71 related diseases, which is worrying considering the recent Asian outbreaks. Also, a significant location-specific difference in seropositivity was observed. Neutralizing antibodies to EV71 were observed in 8.7 percent (21/241) of São Paulo metropolitan area sera samples, and 16.9 percent (34/201) of the sera samples from other municipalities. A high number of Brazilian residents live in country and coastal areas without adequate access to piped water or sanitation. This situation may contribute to the EV71 dissemination in these zones. The analysis of environmental samples could possibly make a valuable contribution to studies on the epidemiology of EV71.

Anticorpos para Enterovírus 71 (EV71) foram avaliados no Estado de São Paulo durante 1999-2005. O título de anticorpos neutralizantes contra EV71 foi determinado pelo ensaio microneutralização, e um título de > 1:8 foi definido como indicador de imunidade protetora. Anticorpos neutralizantes para EV71 foram observados em 12,4 por cento (55/442) das amostras de soro, uma baixa taxa de proteção, sugerindo que a infecção pelo EV71 é incomum nesta região e que existe alta susceptibilidade a doenças relacionadas ao EV71, o que é preocupante considerando os recentes surtos asiáticos. Ainda, foi observada diferença significativa na soropositividade em relação à localização, onde 8,7 por cento (21/241) e 16,9 por cento (34/201) das amostras provenientes da região metropolitana de São Paulo, e demais municípios, respectivamente, apresentaram anticorpos neutralizantes para EV71. Um grande número de brasileiros vive em áreas rurais e à beira-mar, sem acesso adequado à água encanada ou saneamento. Essa situação pode contribuir para a disseminação de EV71 nessas regiões. A análise de amostras ambientais poderia gerar contribuição valiosa para estudos sobre a epidemiologia da EV71.
Descritores: Anticorpos Neutralizantes/sangue
Anticorpos Antivirais/sangue
Enterovirus Humano A/imunologia
Infecções por Enterovirus/virologia
-Brasil/epidemiologia
Infecções por Enterovirus/diagnóstico
Infecções por Enterovirus/epidemiologia
Testes de Neutralização
Estudos Retrospectivos
Limites: Adolescente
Criança
Pré-Escolar
Humanos
Lactente
Recém-Nascido
Adulto Jovem
Responsável: BR1.1 - BIREME



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