Base de dados : LILACS
Pesquisa : B05.237 [Categoria DeCS]
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Id: biblio-1055392
Autor: Rezamahalleh, Hossein Moazzen; Khodakaramian, Gholam; Hassanzadeh, Nader.
Título: Diversity of Endophytic and Epiphytic Bacteria From Sugarcane in Khuzestan, Iran
Fonte: Braz. arch. biol. technol;62:e19180407, 2019. tab, graf.
Idioma: en.
Resumo: Abstract Diverse microorganisms are living as endophytes in plant tissues and as epiphytes on plant surfaces in nature. Commercial formulations of bacteria antagonist to plant pathogenic microbes and ice nucleation active bacteria have been utilized as an environmentally safe method to manage plant disease and to prevent frost damage respectively. Bacteria were isolated from the leaf and sheath of sugarcane (CP69-1026 CP57-614, CP48-103, CP73-21, and CP70-1143 cultivars) verities grown in the field in Khuzestan province, Iran. Bacteria were found in both sheaths and leaves of sugarcane plants which they were significantly higher in density in leaves and which most were endophytic. The bacterial strains were 10 groups on the basis of the biochemical characteristic, which their 16S rRNA encoding gene from representatives were amplified and subjected to sequencing. Results of sequences analyze using blast software from the NCBI website and phylogenetic analysis showed that the representative strains belonged to a wide variety of phylogenetic groups. These results indicated that they were closely related to Burkholderia and Ralstonia from β-Proteobacteria, Mesorhizobium, Ochrobactrum, Sphingomonas from α-Proteobacteria, Microbacterium, Curtobacterium and Leifsonia from Actinobacteria and Xanthomonas from γ-Proteobacteria. This is the first report of the presence of endophytic and epiphytic bacteria from sugarcane in Khuzestan, Iran.
Descritores: RNA Ribossômico 16S
Saccharum/microbiologia
Endófitos
-Filogenia
Responsável: BR1.1 - BIREME


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Id: biblio-1290644
Autor: Feitosa, André de O; Dias, Amanda Cristina S; Ramos, Gisele da C; Bitencourt, Heriberto R; Siqueira, José Edson S; Marinho, Patrícia Santana B; Barison, Andersson; Ocampos, Fernanda M. M; Marinho, Adrey Moacir do R.
Título: Letalidad de citocalasina B y otros compuestos aislados del hongo Aspergillus spp. (Trichocomaceae) endófito de Bauhinia guianensis (Fabaceae) / Lethality of cytochalasin B and other compounds isolated from fungus Aspergillus sp. (Trichocomaceae) endophyte of Bauhinia guianensis (Fabaceae)
Fonte: Rev. argent. microbiol;48(3):259-263, set. 2016. ilus..
Idioma: en.
Resumo: Los hongos endofíticos son hongos que colonizan los tejidos internos de las plantas; varios compuestos biológicamente activos se han aislado a partir de estos hongos. Existen pocos estudios de compuestos aislados de hongos endófitos de plantas amazónicas. Por lo tanto, este estudio tuvo como objetivo el aislamiento y la identificación estructural de ergosterol (1), peróxido de ergosterol (2), mevalonolactona (3), citocalasina B (4) y citocalasina H (5) a partir de Aspergillus spp. EJC 04, un hongo endofítico de Bauhinia guianensis. La citocalasina B (4) y el derivado diacetato de citocalasina B (4a) mostraron una alta letalidad en el ensayo de Artemia salina. Esta es la primera aparición de citocalasinas en hongos endófitos amazónica de B. guianensis

Endophytic fungi are fungi that colonize internal tissues of plants; several biologically active compounds have been isolated from these fungi. There are few studies of compounds isolated from endophytic fungi of Amazon plants. Thus, this study aimed the isolation and structural identification of ergosterol (1), ergosterol peroxide (2), mevalonolactone (3), cytochalasin B (4) and cytochalasin H (5) from Aspergillus sp. EJC 04, an endophytic fungus from Bauhinia guianensis. The cytochalasin B (4) and the diacetate derivative of cytochalasin B (4a) showed high lethality in the brine shrimp assay. This is the first occurrence of cytochalasins in Amazonian endophytic fungi from B. guianensis
Descritores: Artemia/efeitos dos fármacos
Aspergillus/imunologia
Citocalasina B/isolamento & purificação
Citocalasina B/análise
Citocalasinas/isolamento & purificação
Bauhinia/microbiologia
Ergosterol/isolamento & purificação
Endófitos/patogenicidade
Tipo de Publ: Estudo de Avaliação
Estudo Observacional
Responsável: AR635.1 - FCVyS - Servicio de Información y Documentación


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Id: biblio-1132156
Autor: Monteiro, Mônica Cristina Pereira; Tavares, Dérica Gonçalves; Nery, Eduardo Mateus; Queiroz, Marisa Vieira de; Pereira, Olinto Liparini; Cardoso, Patrícia Gomes.
Título: Enzyme Production by Induratia spp. Isolated from Coffee Plants in Brazil
Fonte: Braz. arch. biol. technol;63:e20180673, 2020. tab.
Idioma: en.
Projeto: Foundation of Research Support of Minas Gerais State.
Resumo: Abstract Endophytic fungi belonging to the genus Muscodor now transferred to Induratia are known producers of bioactive volatile organic compounds (VOCs) with many industrial applications. However, the members of this genus have rarely been reported to produce non-volatile metabolites including enzyme. Enzymes of the endophytes are degraders of the polysaccharides available in the host plants and the knowledge of enzyme production by Induratia spp. may provide insights into their possible biotechnological applications. The aim of this study was to evaluate the activity of amylase, cellulase, lipase, pectinase, phytase, protease, endo β-1,4 glucanase and exo β-1,4 glucanase enzymes produced by fungi of the species Induratia coffeana, Induratia yucatanensis and Induratia sp. isolated from organic coffee plants. All Induratia spp. were able to produce the extracellular enzymes cellulase, pectinase, protease, and phytase. Eight fungi were able to produce lipase and four produced amylase. The specific activity of endo β-1, 4 glucanase and exo β-1,4 glucanase enzymes were detected for 9 and 8 endophytic fungi, respectively. This work demonstrated for the first time, the array of enzymes produced by Induratia spp. isolated from Coffea arabica in organic systems in Brazil.
Descritores: Coffea/microbiologia
Ativação Enzimática
Compostos Orgânicos Voláteis/metabolismo
Endófitos/enzimologia
-Brasil
Responsável: BR1.1 - BIREME


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Id: biblio-1132249
Autor: Oki, Yumi; Arantes-Garcia, Lucas; Costa, Marina Batista Morais; Nunes, Bárbara Cristina; Silveira, Bárbara Rúbia; Gélvez-Zúñiga, Irene; Franco, Augusto; Fernandes, Geraldo Wilson.
Título: CO2 Fertilizer Effect on Growth, Polyphenols, and Endophytes in Two Baccharis Species
Fonte: Braz. arch. biol. technol;63:e20190302, 2020. tab.
Idioma: en.
Resumo: Abstract In a climate change context, the buildup of CO2 will affect plant communities worldwide. This study evaluated the effects of CO2 enrichment on the development and defense of two Cerrado native species Baccharis dracunculifolia and B. platypoda and their associated endophytic fungi richness. The study took place in Open-Top Chambers, two with ambient CO2 concentration (~400 ppm) and two in an enriched environment (~800 ppm). Baccharis platypoda developed 20% more leaves under enriched CO2 conditions, whereas B. dracunculifolia was 30% taller and showed 27% more leaves than those under ambient conditions. In both species, leaf polyphenol concentration did not differ between treatments. Nevertheless, polyphenol content had a positive correlation with plant height on both species' individuals grown under CO2 enriched conditions. Endophytic fungi richness and colonization rate on both plant species did not differ between ambient and enriched conditions. Our results show the positive effect of CO2 fertilizer in at least one of the measured growth parameters. An important new finding was a synergistic increase in growth and chemical defense in both studied species under enriched CO2 conditions, suggesting higher carbon assimilation and accumulation. This study suggests that the effects on primary productivity and secondary metabolites of Baccharis species will potentially reflect on the diversity and distribution of Cerrado plants and their associated animal communities.
Descritores: Dióxido de Carbono/farmacologia
Baccharis/crescimento & desenvolvimento
Fertilizantes
Endófitos/crescimento & desenvolvimento
Polifenóis/biossíntese
-Baccharis/microbiologia
Baccharis/química
Responsável: BR1.1 - BIREME


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Azevedo, Joäo Lucio
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Id: biblio-1147925
Autor: Orlandelli, Ravely Casarotti; Almeida, Tiago Tognolli de; Lourenco, Daniela Andressa Lino; Vasconcelos, Ana Flora Dalberto; Silva, Maria de Lourdes Corradi da; Azevedo, João Lúcio; Pamphile, João Alencar.
Título: Toxicity of ß-(1â†'3, 1â†'6)-á´…-glucans produced by Diaporthe sp. endophytes on Metarhizium anisopliae (Metschnikoff) Sorokin assessed by conidia germination speed parameter / Toxicidade de ß- (1 â†' 3, 1 â†' 6) -á´…-glucanas produzidas por endófitos Diaporthe sp. sobre Metarhizium anisopliae (Metschnikoff) Sorokin avaliada pelo parâmetro de velocidade de germinação dos conídios
Fonte: Biosci. j. (Online);36(5):1742-1749, 01-09-2020. tab, graf.
Idioma: en.
Resumo: We have previously reported that ß-(1â†'3,1â†'6)-á´…-glucans produced by endophytes Diaporthe sp. G27-60 and G65-65 (GenBank accession codes JF766998 and JF767007, respectively) are promising anti-proliferation agents against human breast carcinoma (MCF-7) and hepatocellular carcinoma (HepG2-C3A) cells. However, the literature fails to describe the effects of Diaporthe exopolysaccharides (EPS) on eukaryotic healthy cells. The fungus Metarhiziumanisopliae has been employed as model-system to evaluate the toxicity of pharmaceutical and agricultural-interest substances, taking into account, among other parameters, the speed of conidia germination. Current study verified the effect of different concentrations of Diaporthe ß-glucans on the germination speed of M. anisopliae. Conidia were incubated with ß-glucans treatments (50, 200 and 400 µg/mL) at 28ºC, sampled during 24 h and analyzed by light microscopy. At the end of a 24-h incubation, the amount of germinated conidia reached ≈99% for controls and ranged between 97.7 and 98.6% for treatments. Bayesian analysis indicated that Diaporthe glucans had no toxicity on M. anisopliaeand the curve of germination occurred as expected for this fungal strain. Considering the validity of filamentous fungi as model-systems, results are important data on the toxicity of endophytic EPS on healthy cells and may be associated with our previous results obtained for these polymers against tumor cells.

Anteriormente, um estudo mostrou que ß-(1â†'3,1â†'6)-á´…-glucanas produzidas pelos endófitos Diaporthe sp. G27-60 e G65-65 (códigos de acesso no GenBank JF766998 e JF767007, respectivamente) são agentes promissores com ação antiproliferativa contra células HepG2-C3A (hepatoma humano) e MCF-7 (adenocarcinoma mamário humano). No entanto, os efeitos de exopolissacarídeos (EPS) produzidos por fungos do gênero Diaporthe em células eucarióticas sadias não estão descritos na literatura atual. O fungo Metarhiziumanisopliae tem sido utilizado como sistema-modelo para avaliar a toxicidade de substâncias de interesse farmacêutico e agronômico, considerando, entre outros parâmetros, a velocidade de germinação de conídios. O presente estudo teve como objetivo verificar os efeitos de diferentes concentrações de ß-glucanas produzidas por Diaporthe sp. sobre a velocidade de germinação de M. anisopliae. Os conídios foram incubados com os tratamentos de ß-glucanas (50, 200 e 400 µg/mL) a 28 ºC, com amostras coletadas ao longo de 24 h, e analisados por microscopia de luz. Ao final das 24 h de incubação, o total de conídios germinados nos controles foi de ≈99%, e variou entre 97,7 e 98,6% para os tratamentos. A análise bayesiana indicou que as glucanas de Diaporthe sp. não apresentaram toxicidade sobre M. anisopliae, e a curva de germinação atendeu ao esperado para essa linhagem fúngica. Considerando a validade dos fungos filamentosos como sistemas-modelo, esses resultados representam dados importantes sobre a toxicidade dos EPS de endófitos sobre células sadias e podem ser associados aos resultados anteriormente obtidos para esses polímeros em testes contra células tumorais.
Descritores: Teorema de Bayes
Endófitos
Fungos
Responsável: BR396.1 - Biblioteca Central


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Id: lil-394078
Autor: Souza, Antonia Queiroz Lima de; Souza, Afonso Duarte Leão de; Astolfi Filho, Spartaco; Pinheiro, Maria Lúcia Belém; Sarquis, Maria Inez de Moura; Pereira, José Odair.
Título: Atividade antimicrobiana de fungos endofíticos isolados de plantas tóxicas da amazônia: Palicourea longiflora (aubl. ) rich e Strychnos cogens bentham / Antimicrobial activity of endophytic fungi isolated from amazonian toxic plants: Palicourea longiflora (aubl. ) rich and Strychnos cogens bentham
Fonte: Acta amaz;34(2):185-195, 2004. ilus, tab, graf.
Idioma: pt.
Resumo: Das plantas tóxicas da Amazônia Palicourea longiflora e Strychnos cogens foram isolados 571 fungos endofíticos e 74 bactérias endofíticas. Palicourea longiflora (Rubiaceae) e outras espécies desse gênero estão relacionadas a 90 por cento das mortes de gado na região Amazônica. Strychnos cogens (Loganiaceae) e outras espécies de Strychnos são utilizadas pelos indígenas na confecção de "curares". Entre os endófitos isolados de P. longiflora foram identificados os fungos: Colletotrichum sp. e seu telemorfo Glomerella sp., Guignardia sp., Aspergillus niger, Phomopsis sp. e Xylaria sp., além da bactéria Burkholderia gladioli, pertencente a um grupo de fixadoras de nitrogênio. Dos isolados de S. cogens foram identificados os fungos: Colletotrichum sp., Guignardia sp., Aspergillus niger e Trichoderma sp. Uma amostra de 79 isolados fúngicos teve seus metabólitos extracelulares bioensaiados contra microrganismos patogênicos e fitopatogênicos: 19 isolados inibiram um ou mais microrganismos-teste. Dos oito isolados com melhores resultados de inibição, as móleculas bioativas eram menores que 12.000 daltons, fato verificado pela diálise dos metabólitos.
Descritores: Bactérias
Bioensaio
Rubiaceae
Strychnos
Endófitos
Fungos
Responsável: BR6.1 - BCS - Biblioteca de Ciências da Saúde


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Id: biblio-886833
Autor: MATOS, AMANDA D M; GOMES, IZABELA C P; NIETSCHE, SILVIA; XAVIER, ADELICA A; GOMES, WELLINGTON S; DOS SANTOS NETO, JOSÉ A; PEREIRA, MARLON C T.
Título: Phosphate solubilization by endophytic bacteria isolated from banana trees
Fonte: An. acad. bras. ciênc;89(4):2945-2954, Oct.-Dec. 2017. tab, graf.
Idioma: en.
Resumo: ABSTRACT Forty isolates of endophytic bacteria isolated from banana tree roots were assessed as to their capacity to solubilize phosphate in a solid culture medium supplemented with different inorganic and one organic source of phosphorus. The amount of phosphorus (P) in each liquid medium was quantified, and an indirect assessment of acid phosphatase activity was performed. All assays had a fully randomized design, with three repetitions. Approximately 67.5% of the 40 isolates assessed in solid medium solubilized phosphorus from tricalcium phosphate and 7.5% of the isolates solubilized phosphorus from soy lecithin; no isolates exhibited P solubilization capacity in medium supplemented with iron phosphate. Acid phosphatase activity was detected in 65% of the isolates; Aneurinibacillus sp. and Lysinibacillus sp. isolates presented with the best solubilization indexes. All of the assessed isolates exhibited a capacity to reduce the potential of hydrogen in liquid medium supplemented with tricalcium phosphate. Isolate EB. 78 (Bacillus sp.) exhibited P solubilization capacity in solid media when Ca3(PO4)2 and soy lecithin were used as P sources; this isolate significantly reduced the pH of the liquid medium and exhibited acid phosphatase activity. The results of the present study highlight isolates that exhibit variations in their capacity to solubilize P. These isolates should be used in future tests to assess their field performance.
Descritores: Fosfatos/metabolismo
Bactérias/metabolismo
Biodegradação Ambiental
Musa/microbiologia
Endófitos/fisiologia
-Bactérias/classificação
Responsável: BR1.1 - BIREME


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Id: biblio-1089298
Autor: Li, F; He, X; Tang, M; Tang, X; Liu, J; Yi, Y.
Título: Adaptation of plants to high-calcium content kart regions: possible involvement of symbiotic microorganisms and underlying mechanisms / Adaptação de plantas a regiões de kart com alto teor de cálcio: possível envolvimento de microrganismos simbióticos e mecanismos subjacentes
Fonte: Braz. j. biol;80(1):209-214, Feb. 2020. graf.
Idioma: en.
Projeto: Guizhou Natural Science Foundation; . National Natural Science Foundation of China.
Resumo: Abstract Rhizosphere microorganisms and endophytes can help their hosts absorb nutrients and regulate the levels of plant hormones. Moreover, they can modulate the expressions of host genes, assist hosts in eliminating reactive oxygen species (ROS) and secreting volatile organic compounds. Therefore, rhizosphere microorganisms and endophytes are considered as determinant factors driving processes involved in the growth of host plants. However, the physiological and ecological functions, as well as the molecular mechanism underlying the behavior of rhizosphere microorganisms and endophytes in their role in the adaptive capacity of host plants in the karstic high-calcium environment have not been systematically studied. This review summarizes the physiological and molecular mechanisms of rhizosphere microorganisms and endophytes which help host plants to adapt to various kinds of adverse environments. The adaptive capacities of plants growing in adverse environments, partly, or totally, depends on microorganisms co-existing with the host plants.

Resumo Os microorganismos e endófitos da rizosfera podem ajudar seus hospedeiros a absorver nutrientes e regular os níveis de hormônios vegetais. Além disso, eles podem modular as expressões dos genes hospedeiros, auxiliar os hospedeiros na eliminação de espécies reativas de oxigênio (ROS) e secretar compostos orgânicos voláteis. Portanto, microorganismos e endófitos da rizosfera são considerados determinantes dos processos envolvidos no crescimento de plantas hospedeiras. No entanto, as funções fisiológicas e ecológicas, bem como o mecanismo molecular subjacente ao comportamento dos microrganismos e endofíticos da rizosfera no seu papel na capacidade adaptativa das plantas hospedeiras no ambiente cárstico de alto teor de cálcio, não foram sistematicamente estudados. Esta revisão resume os mecanismos fisiológicos e moleculares de microrganismos e endófitos da rizosfera que ajudam as plantas hospedeiras a se adaptarem a vários tipos de ambientes adversos. As capacidades adaptativas das plantas que crescem em ambientes adversos, em parte ou totalmente, dependem de microrganismos coexistentes com as plantas hospedeiras.
Descritores: Simbiose
Cálcio
-Plantas
Rizosfera
Endófitos
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME


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Id: biblio-974328
Autor: Yang, Guodong; Li, Peng; Meng, Lifen; Xv, Keyi; Dong, Faming; Qiu, Yan; He, Lei; Lin, Lin.
Título: Diversity and communities of culturable endophytic fungi from different tree peonies (geoherbs and non-geoherbs), and their biosynthetic potential analysis
Fonte: Braz. j. microbiol;49(supl.1):47-58, 2018. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China.
Resumo: Abstract To know more about the potential roles of endophytic fungi in the formation mechanism of Daodi medicinal material, diversity and communities of culturable endophytic fungi in three types of tree peonies were investigated. Endophytic fungi of three types of tree peonies were isolated and identified. The diversity was analyzed. Bayesian trees constructed by MrBayes 3.2.6 after phylogenetic analysis of the ITS sequences. The endophytic fungi potential for synthesis of natural products was assessed by means of detecting NRPS and PKS gene sequences. In total, 364 endophytic fungi isolates representing 26 genera were recovered from Paeonia ostii 'Feng Dan', Paeonia ostii 'Luoyang Feng Dan', and Paeonia suffruticosa 'Luoyang Hong'. More culturable endophytic fungi appeared in P. suffruticosa 'Luoyang Hong' (206) compared with P. ostii 'Feng Dan' (60) and P. ostii 'Luoyang Feng Dan' (98). The fungal community of P. ostii 'Feng Dan' had the highest richness and diversity. PKSs and NRPS detection rates of endophytic fungi from P. ostii 'Feng Dan' are both the highest among the three types of tree peonies. Results indicate that endophytic fungus is an important factor of Daodi Cortex Moutan forming, and endophytic fungi in peony are related to genuineness of Cortex Moutan.
Descritores: Produtos Biológicos/metabolismo
Paeonia/microbiologia
Biodiversidade
Endófitos/isolamento & purificação
Fungos/isolamento & purificação
-Filogenia
Medicamentos de Ervas Chinesas/metabolismo
Paeonia/classificação
Paeonia/crescimento & desenvolvimento
Paeonia/metabolismo
Endófitos/classificação
Endófitos/crescimento & desenvolvimento
Endófitos/genética
Fungos/classificação
Fungos/crescimento & desenvolvimento
Fungos/genética
Responsável: BR1.1 - BIREME


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Azevedo, Joäo L
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Id: biblio-974284
Autor: Elias, Luciana M; Fortkamp, Diana; Sartori, Sérgio B; Ferreira, Marília C; Gomes, Luiz H; Azevedo, João L; Montoya, Quimi V; Rodrigues, André; Ferreira, Antonio G; Lira, Simone P.
Título: The potential of compounds isolated from Xylaria spp. as antifungal agents against anthracnose
Fonte: Braz. j. microbiol;49(4):840-847, Oct.-Dec. 2018. graf.
Idioma: en.
Projeto: FAPESP; . CNPq.
Resumo: ABSTRACT Anthracnose is a crop disease usually caused by fungi in the genus Colletotrichum or Gloeosporium. These are considered one of the main pathogens, causing significant economic losses, such as in peppers and guarana. The current forms of control include the use of resistant cultivars, sanitary pruning and fungicides. However, even with the use of some methods of controlling these cultures, the crops are not free of anthracnose. Additionally, excessive application of fungicides increases the resistance of pathogens to agrochemicals and cause harm to human health and the environment. In order to find natural antifungal agents against guarana anthracnose, endophytic fungi were isolated from Amazon guarana. The compounds piliformic acid and cytochalasin D were isolated by chromatographic techniques from two Xylaria spp., guided by assays with Colletotrichum gloeosporioides. The isolated compounds were identified by spectrometric techniques, as NMR and mass spectrometry. This is the first report that piliformic acid and cytochalasin D have antifungal activity against C. gloeosporioides with MIC 2.92 and 2.46 µmol mL-1 respectively. Captan and difenoconazole were included as positive controls (MIC 16.63 and 0.02 µmol mL-1, respectively). Thus, Xylaria species presented a biotechnological potential and production of different active compounds which might be promising against anthracnose disease.
Descritores: Doenças das Plantas/prevenção & controle
Xylariales/química
Paullinia/microbiologia
Endófitos/química
Fungicidas Industriais/farmacologia
-Filogenia
Doenças das Plantas/microbiologia
Espectrometria de Massas
Xylariales/isolamento & purificação
Xylariales/genética
Xylariales/metabolismo
Estrutura Molecular
Colletotrichum/efeitos dos fármacos
Colletotrichum/fisiologia
Endófitos/isolamento & purificação
Endófitos/genética
Endófitos/metabolismo
Fungicidas Industriais/isolamento & purificação
Fungicidas Industriais/química
Responsável: BR1.1 - BIREME



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