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Pesquisa : B05.256 [Categoria DeCS]
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Id: biblio-991398
Autor: Arce, Zhandra; Arias, Alejandra; Mera, Astrid.
Título: Organismos extremófilos en el ojo de la ciencia / Extremophile organisms in the eye of science
Fonte: Rev. méd. hered;28(1):70-71, ene. 2017.
Idioma: es.
Descritores: Tardígrados
Limites: Humanos
Tipo de Publ: Carta
Responsável: PE1.1 - Oficina Universitária de Biblioteca

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Id: biblio-1016090
Autor: Castro, Daniela E; Murguía-Romero, Miguel; Thomé, Patricia E; Peña, Antonio; Calderón-Torres, Marissa.
Título: Putative 3-nitrotyrosine detoxifying genes identified in the yeast Debaryomyces hansenii: in silico search of regulatory sequences responsive to salt and nitrogen stress
Fonte: Electron. j. biotechnol;29:1-6, sept. 2017. graf, tab.
Idioma: en.
Projeto: DGAPA-UNAM.
Resumo: Background: During salt stress, the yeast Debaryomyces hansenii synthesizes tyrosine as a strategy to avoid the oxidation of proteins. Tyrosine reacts with nitrogen radicals to form 3-nitrotyrosine. 3-nitrotyrosine prevents the effects of associated oxidative stress and thus contributes to the high halotolerace of the yeast. However, the mechanism of how D. hansenii counteracts the presence of this toxic compound is unclear. In this work, we evaluated D. hansenii's capacity to assimilate 3-nitrotyrosine as a unique nitrogen source and measured its denitrase activity under salt stress. To identify putative genes related to the assimilation of 3-nitrotyrosine, we performed an in silico search in the promoter regions of D. hansenii genome. Results: We identified 15 genes whose promoters had binding site sequences for transcriptional factors of sodium, nitrogen, and oxidative stress with oxidoreductase and monooxygenase GO annotations. Two of these genes, DEHA2E24178g and DEHA2C00286g, coding for putative denitrases and having GATA sequences, were evaluated by RT-PCR and showed high expression under salt and nitrogen stress. Conclusions: D. hansenii can grow in the presence of 3-nitrotyrosine as the only nitrogen source and has a high specific denitrase activity to degrade 3-nitrotyrosine in 1 and 2 M NaCl stress conditions. The results suggest that given the lack of information on transcriptional factors in D. hansenii, the genes identified in our in silico analysis may help explain 3-nitrotyrosine assimilation mechanisms.
Descritores: Tirosina/análogos & derivados
Transcrição Genética
Sequências Reguladoras de Ácido Nucleico
Regiões Promotoras Genéticas
Estresse Oxidativo
Reação em Cadeia da Polimerase em Tempo Real
Responsável: CL1.1 - Biblioteca Central

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Id: biblio-1008708
Autor: Valdebenito-Rolack, Emky; Ruiz-Tagle, Nathaly; Abarzúa, Leslie; Aroca, Germán; Urrutia, Homero.
Título: Characterization of a hyperthermophilic sulphur-oxidizing biofilm produced by archaea isolated from a hot spring
Fonte: Electron. j. biotechnol;25:58-63, ene. 2017. tab, graf, ilus.
Idioma: en.
Projeto: FONDECYT.
Resumo: Background: Sulphur-oxidizing microorganisms are widely used in the biofiltration of total reduced sulphur compounds (odorous and neurotoxic) produced by industries such as the cellulose and petrochemical industries, which include high-temperature process steps. Some hyperthermophilic microorganisms have the capability to oxidize these compounds at high temperatures (N60°C), and archaea of this group, for example, Sulfolobus metallicus, are commonly used in biofiltration technology. Results: In this study, a hyperthermophilic sulphur-oxidizing strain of archaea was isolated from a hot spring (Chillán, Chile) and designated as M1. It was identified as archaea of the genus Sulfolobus (99% homology with S. solfataricus 16S rDNA). Biofilms of this culture grown on polyethylene rings showed an elemental sulphur oxidation rate of 95.15 ± 15.39 mg S l-1 d-1, higher than the rate exhibited by the biofilm of the sulphur-oxidizing archaea S. metallicus (56.8 ± 10.91 mg l-1 d-1). Conclusions: The results suggest that the culture M1 is useful for the biofiltration of total reduced sulphur gases at high temperatures and for other biotechnological applications.
Descritores: Sulfetos/metabolismo
Reação em Cadeia da Polimerase
Archaea/isolamento & purificação
Fontes Termais/microbiologia
Temperatura Alta
Responsável: CL1.1 - Biblioteca Central

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