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  1 / 2551 LILACS  
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Id: biblio-1142578
Autor: Viana, G S B; Vale, E M do; Araujo, A R A de; Coelho, N C; Andrade, S M; Costa, R O da; Aquino, P E A de; Sousa, C N S de; Medeiros, I S de; Vasconcelos, S M M de; Neves, K R T.
Título: Rapid and long-lasting antidepressant-like effects of ketamine and their relationship with the expression of brain enzymes, BDNF, and astrocytes
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;54(2):e10107, 2021. graf.
Idioma: en.
Resumo: Ketamine (KET) is an N-methyl-D-aspartate (NMDA) antagonist with rapid and long-lasting antidepressant effects, but how the drug shows its sustained effects is still a matter of controversy. The objectives were to evaluate the mechanisms for KET rapid (30 min) and long-lasting (15 and 30 days after) antidepressant effects in mice. A single dose of KET (2, 5, or 10 mg/kg, po) was administered to male Swiss mice and the forced swim test (FST) was performed 30 min, 15, or 30 days later. Imipramine (IMI, 30 mg/kg, ip), a tricyclic antidepressant drug, was used as reference. The mice were euthanized, separated into two time-point groups (D1, first day after KET injection; D30, 30 days later), and brain sections were processed for glycogen synthase kinase-3 (GSK-3), histone deacetylase (HDAC), brain-derived neurotrophic factor (BDNF), and glial fibrillary acidic protein (GFAP) immunohistochemical assays. KET (5 and 10 mg/kg) presented rapid and long-lasting antidepressant-like effects. As expected, the immunoreactivities for brain GSK-3 and HDAC decreased compared to control groups in all areas (striatum, DG, CA1, CA3, and mainly pre-frontal cortex, PFC) after KET injection. Increases in BDNF immunostaining were demonstrated in the PFC, DG, CA1, and CA3 areas at D1 and D30 time-points. GFAP immunoreactivity was also increased in the PFC and striatum at both time-points. In conclusion, KET changed brain BDNF and GFAP expressions 30 days after a single administration. Although neuroplasticity could be involved in the observed effects of KET, more studies are needed to explain the mechanisms for the drug's sustained antidepressant-like effects.
Descritores: Encéfalo/efeitos dos fármacos
Encéfalo/enzimologia
Fator Neurotrófico Derivado do Encéfalo/metabolismo
Ketamina/farmacologia
Antidepressivos/farmacologia
-Astrócitos
Quinase 3 da Glicogênio Sintase
Modelos Animais de Doenças
Proteína Glial Fibrilar Ácida
Histona Desacetilases
Limites: Animais
Masculino
Coelhos
Responsável: BR1.1 - BIREME


  2 / 2551 LILACS  
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Id: biblio-1142583
Autor: Mosini, A C; Calió, M L; Foresti, M L; Valeriano, R P S; Garzon, E; Mello, L E.
Título: Modeling of post-traumatic epilepsy and experimental research aimed at its prevention
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;54(2):e10656, 2021. graf.
Idioma: en.
Projeto: FAPESP; . CNPq.
Resumo: Research on the prevention of post-traumatic epilepsy (PTE) has seen remarkable advances regarding its physiopathology in recent years. From the search for biomarkers that might be used to indicate individual susceptibility to the development of new animal models and the investigation of new drugs, a great deal of knowledge has been amassed. Various groups have concentrated efforts in generating new animal models of traumatic brain injury (TBI) in an attempt to provide the means to further produce knowledge on the subject. Here we forward the hypothesis that restricting the search of biomarkers and of new drugs to prevent PTE by using only a limited set of TBI models might hamper the understanding of this relevant and yet not preventable medical condition.
Descritores: Epilepsia Pós-Traumática/etiologia
Epilepsia Pós-Traumática/prevenção & controle
Modelos Animais de Doenças
Lesões Encefálicas Traumáticas/complicações
Lesões Encefálicas Traumáticas/prevenção & controle
-Biomarcadores
Limites: Animais
Responsável: BR1.1 - BIREME


  3 / 2551 LILACS  
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Id: biblio-1153521
Autor: Huang, Xue-peng; Qin, Cheng-yu; Gao, Yue-ming.
Título: miR-135a inhibits airway inflammatory response in asthmatic mice via regulating JAK/STAT signaling pathway
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;54(3):e10023, 2021. graf.
Idioma: en.
Resumo: The objective of this study was to investigate the inhibitory effect of miR-135a in regulating JAK/STAT signaling pathway on airway inflammation in asthmatic mice. An asthma model was established by sensitization and stimulation with ovalbumin (OVA), and the corresponding drug intervention was given from the day of stimulation by means of nasal drops. Airway hyperresponsiveness was tested. The content of miR-135a in the lung tissue of mice was detected by RT-PCR. The pathological changes of lung tissue were evaluated by HE staining. Tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-5, and eotaxin in bronchoalveolar lavage fluid (BALF) and lung tissue were detected by ELISA and immunohistochemistry, respectively. The expression of JAK/STAT signaling pathway-related protein in lung tissue was detected by western blot. To further validate the effect of miR-135a overexpression on the JAK/STAT signaling pathway, pathway activators and inhibitors were added. Compared with the OVA group, the airway hyperresponsiveness of the mice was significantly decreased after treatment with the miR-135a agonist. The expression of miR-135a was significantly increased in the lung tissue and the pathological changes of the lung tissue were alleviated. The contents of TNF-α, IL-6, IL-5, and eotaxin in BALF and lung tissues were decreased. The expression of JAK/STAT signaling pathway-related proteins p-JAK3/JAK3, p-STAT1/STAT1, and p-STAT3/STAT3 were significantly reduced in lung tissue (P<0.05). Addition of JAK inhibitor AG490 reduced airway inflammation in asthmatic mice. miR-135a agonists inhibit airway inflammation in asthmatic mice by regulating the JAK/STAT signaling pathway.
Descritores: Asma/tratamento farmacológico
-Líquido da Lavagem Broncoalveolar
Transdução de Sinais
Ovalbumina
MicroRNAs
Modelos Animais de Doenças
Pulmão
Camundongos Endogâmicos BALB C
Limites: Animais
Ratos
Responsável: BR1.1 - BIREME


  4 / 2551 LILACS  
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Bernardi, Maria Martha
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Id: biblio-952015
Autor: Cirano, Fabiano Ribeiro; Casarin, Renato Correa Viana; Ribeiro, Fernanda Vieira; Casati, Marcio Zaffalon; Pimentel, Suzana Peres; Taiete, Tiago; Bernardi, Maria Martha.
Título: Effect of Resveratrol on periodontal pathogens during experimental periodontitis in rats
Fonte: Braz. oral res. (Online);30(1):e128, 2016. tab, graf.
Idioma: en.
Projeto: São Paulo Research Foundation; . National Counsel of Technological and Scientific Development; . National Counsel of Technological and Scientific Development.
Resumo: Abstract The objective of this study was to investigate the antibacterial effect of resveratrol against putative periodontal pathogens during the progression of experimental periodontitis in rats. Periodontitis was induced in rats in one of the first molars chosen to receive a ligature. Animals were assigned to one of two groups: daily administration of the placebo solution (control group, n = 12) or 10 mg/Kg of resveratrol (RESV group, n = 12). The therapies were administered systemically for 30 days, for 19 days before periodontitis induction and then for another 11 days. Then, the presence and concentrations of Porphyromonas gingivalis, Tannerella forsythia and Aggregatibacter actinomycetemcomitans in the cotton ligatures collected from the first molars were evaluated using real-time PCR. Inter-group comparisons of the microbiological outcomes revealed that no differences were detected for P. gingivalis, T. forsythia and A. actinomycetemcomitans levels (p > 0.05). Continuous use of resveratrol did not promote additional benefits in microbiological outcomes during experimental periodontitis in rats.
Descritores: Periodontite/microbiologia
Periodontite/tratamento farmacológico
Estilbenos/farmacologia
Antibacterianos/farmacologia
-Estilbenos/uso terapêutico
Fatores de Tempo
Periodonto/microbiologia
Reprodutibilidade dos Testes
Resultado do Tratamento
Aggregatibacter actinomycetemcomitans
Ratos Wistar
Porphyromonas gingivalis/efeitos dos fármacos
Modelos Animais de Doenças
Reação em Cadeia da Polimerase em Tempo Real
Tannerella forsythia/efeitos dos fármacos
Resveratrol
Antibacterianos/uso terapêutico
Limites: Animais
Ratos
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


  5 / 2551 LILACS  
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Spolidorio, Luis Carlos
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Id: biblio-952039
Autor: Conte Neto, Nicolau; Spolidorio, Luis Carlos; Andrade, Cleverton Roberto de; Esteves, Jônatas Caldeira; Marcantonio Jr, Elcio.
Título: Experimental osteonecrosis: development of a model in rodents administered alendronate
Fonte: Braz. oral res. (Online);30(1):e99, 2016. graf.
Idioma: en.
Resumo: Abstract The main objective of this study was to cause bisphosphonate-related osteonecrosis of the jaws to develop in a rodent model. Adult male Holtzman rats were assigned to one of two experimental groups to receive alendronate (AL; 1 mg/kg/week; n = 6) or saline solution (CTL; n = 6). After 60 days of drug therapy, all animals were subjected to first lower molar extraction, and 28 days later, animals were euthanized. All rats treated with alendronate developed osteonecrosis, presenting as ulcers and necrotic bone, associated with a significant infection process, especially at the inter-alveolar septum area and crestal regions. The degree of vascularization, the levels of C-telopeptide cross-linked collagen type I and bone-specific alkaline phosphatase, as well as the bone volume were significantly reduced in these animals. Furthermore, on radiographic analysis, animals treated with alendronate presented evident sclerosis of the lamina dura of the lower first molar alveolar socket associated with decreased radiographic density in this area. These findings indicate that the protocol developed in the present study opens new perspectives and could be a good starting model for future property design.
Descritores: Alendronato/administração & dosagem
Modelos Animais de Doenças
Conservadores da Densidade Óssea/administração & dosagem
Osteonecrose da Arcada Osseodentária Associada a Difosfonatos/etiologia
-Peptídeos/sangue
Fatores de Tempo
Extração Dentária
Ensaio de Imunoadsorção Enzimática
Biomarcadores/sangue
Densidade Óssea/efeitos dos fármacos
Ratos Sprague-Dawley
Alvéolo Dental/efeitos dos fármacos
Alvéolo Dental/patologia
Colágeno Tipo I/sangue
Fosfatase Alcalina/sangue
Osteonecrose da Arcada Osseodentária Associada a Difosfonatos/diagnóstico por imagem
Limites: Animais
Masculino
Responsável: BR1.1 - BIREME


  6 / 2551 LILACS  
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Id: biblio-952122
Autor: Lin, Mei; Hu, Yang; Wang, Yuhua; Kawai, Toshihisa; Wang, Zuomin; Han, Xiaozhe.
Título: Different engagement of TLR2 and TLR4 in Porphyromonas gingivalis vs. ligature-induced periodontal bone loss
Fonte: Braz. oral res. (Online);31:e63, 2017. graf.
Idioma: en.
Projeto: NIDCR; . NIDCR.
Resumo: Abstract This study was conducted to investigate the roles of different Toll-like receptor (TLR) signaling in Porphyromonas gingivalis (P. gingivalis)-induced and ligature-induced experimental periodontal bone resorption in mice. Wild-type (WT), TLR2 knockout (KO), TLR4KO, and TLR2&4 KO mice with C57/BL6 background were divided into three groups: control, P. gingivalis infection, and ligation. Live P. gingivalis or silk ligatures were placed in the sulcus around maxillary second molars over a 2-week period. Images were captured by digital stereomicroscopy, and the bone resorption area was measured with ImageJ software. The protein expression level of gingival RANKL was measured by ELISA. The gingival mRNA levels of RANKL, IL-1β, TNF-α, and IL-10 were detected by RT-qPCR. The results showed that P. gingivalis induced significant periodontal bone resorption in WT mice and TLR2 KO mice but not in TLR4 KO mice or TLR2&4 KO mice. For all four types of mice, ligation induced significant bone loss compared with that in control groups, and this bone loss was significantly higher than that in the P. gingivalis infection group. RANKL protein expression was significantly increased in the ligation group compared with that in the control group for all four types of mice, and in the P. gingivalis infection group of WT, TLR2 KO, and TLR4 KO mice. Expression patterns of RANKL, IL-1β, TNF-α, and IL-10 mRNA were different in the P. gingivalis infection group and the ligation group in different types of mice. In summary, P. gingivalis-induced periodontal bone resorption is TLR4-dependent, whereas ligation-induced periodontal bone resorption is neither TLR2- nor TLR4-dependent.
Descritores: Periodontite/microbiologia
Perda do Osso Alveolar/etiologia
Porphyromonas gingivalis/patogenicidade
Modelos Animais de Doenças
Receptor 2 Toll-Like/fisiologia
Receptor 2 Toll-Like/genética
Receptor 4 Toll-Like/fisiologia
-Fatores de Tempo
Ensaio de Imunoadsorção Enzimática
Reprodutibilidade dos Testes
Perda do Osso Alveolar/microbiologia
Fator de Necrose Tumoral alfa/metabolismo
Interleucina-10/metabolismo
Camundongos Knockout
Receptor 2 Toll-Like/análise
Receptor 4 Toll-Like/análise
Receptor 4 Toll-Like/genética
Interleucina-1beta/metabolismo
Receptor Ativador de Fator Nuclear kappa-B/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
Ligadura
Metabolismo
Camundongos Endogâmicos C57BL
Limites: Animais
Responsável: BR1.1 - BIREME


  7 / 2551 LILACS  
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Rosa, Fabiana Paim
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Id: biblio-1247808
Autor: Dourado, Raísa Cavalcante; Rolim, Ana Emília Holanda; Rosa, Fabiana Paim.
Título: Uso de marcadores fluorescentes na engenharia tecidual óssea: estudo piloto / Use of fluorescent markers in bone tissue engineering: pilot study
Fonte: Rev. Ciênc. Méd. Biol. (Impr.) = J. med. biol. sci;17(3):359-368, nov 19, 2018. ilus.
Idioma: pt.
Resumo: Introdução: a regeneração e o reparo de tecidos ósseos perdidos é objeto de estudo da Bioengenharia Tecidual. O uso de biomateriais substitutos ósseos biomiméticos visa estimular os sistemas celulares e bioquímicos para restabelecer de modo mais eficiente o tecido ósseo nos casos de sua reconstrução. Ao investigar o processo de remodelação, é vital identificar áreas de novo crescimento para avaliar a eficácia dos biomateriais implantados e respectivos regimes de tratamento. A avaliação qualitativa e quantitativa da regeneração óssea pode ser realizada através da aplicação de marcadores como o Xilenol, a Tetraciclina, a Calceína e a Alizarina. A administração desses marcadores de forma associada possibilita ainda marcar sequencialmente camadas de nova deposição e remodelação durante o reparo. Objetivo: estabelecer um protocolo para utilização dos marcadores fluorescentes de reparo ósseo xilenol, tetraciclina, calceína e alizarina, em ratos. Metodologia: foram utilizados 35 ratos da linhagem Wistar, machos adultos, com massa corpórea entre 350 e 400g, e idade aproximada de 4 a 5 meses, distribuídos randomicamente em 5 grupos experimentais, submetidos à confecção de defeito ósseo circular de 8 mm em região de calvária, e administração dos diferentes marcadores segundo os grupos; XO ­ Xilenol; Ca ­ Calceína; Al ­ Alizarina; Te ­ Tetraciclina; C ­ Controle. Após 15 dias de experimento, os animais foram eutanasiados e as calvárias processadas e analisadas por histomorfometria, microscopia de epifluorescência e microscopia de fluorescência. Resultados: todos protocolos empregados para utilização dos marcadores fluorescentes xilenol, calceína, alizarina e tetracicilina foram úteis para identificar área de deposição mineral durante o período analisado de regeneração óssea em ratos. As imagens obtidas pela microscopia de fluorescência revela a presença dos marcadores incorporados à matriz óssea neoformada, no entanto a utilização da Alizarina e Calceína dentro dos protocolos testados mostraram-se mais eficientes. Conclusão: os protocolos testados nesse estudo apresentaram-se viáveis para utilização em pesquisas envolvendo marcadores de regeneração óssea, com resultados superiores para Alizarina e Calceína

Introduction: The regeneration and repair of lost bone tissues is the subject of a study of Tissue Bioengineering. The use of biomimetic biomaterial bone substitutes aims to stimulate the cellular and biochemical systems to restore more efficiently the bone tissue in the cases of its reconstruction. When investigating the remodeling process, it is vital to identify areas of new growth to evaluate the efficacy of implanted biomaterials and their treatment regimens. The qualitative and quantitative evaluation of bone regeneration can be performed through the use of markers such as Xylenol, Tetracycline, Calcein and Alizarin. The administration of such markers in an associated manner also makes it possible to sequentially mark layers of new deposition and remodeling during the repair. Objective: to establish a protocol for the use of fluorescent xylenol, tetracycline, calcein and alizarin bone repair markers in rats. Metodology: thirtyfive male adult Wistar rats with a body mass ranging from 350 to 400 g and approximately 4 to 5 months old were randomly assigned to 5 experimental groups submitted to a circular bone defect of 8 mm in the region of calvaria, and administration of the different markers according to the groups; XO ­ Xylenol; Ca ­ Calcein; Al-Alizarin; Te ­ Tetracycline; C ­ Control. After 15 days of experiment, the animals were euthanized and the calvaria processed and analyzed by histomorphometry, epifluorescence microscopy and fluorescence microscopy. Results: all protocols used for fluorescence markers xylenol, calcein, alizarin and tetracycline were useful to identify area of mineral deposition during the analyzed period of bone regeneration in rats. The images obtained by fluorescence microscopy revealed the presence of the markers incorporated into the neoformed bone matrix, however the use of Alizarin and Calcein within the protocols tested were more efficient. Conclusion: the protocols tested in this study were feasible for use in research involving markers of bone regeneration, with superior results for Alizarin and Calcein.
Descritores: Regeneração Óssea/efeitos dos fármacos
Engenharia Tecidual/métodos
Corantes Fluorescentes/farmacologia
-Tetraciclina/farmacologia
Xilenos/farmacologia
Distribuição Aleatória
Projetos Piloto
Ratos Wistar
Modelos Animais de Doenças
Microscopia de Fluorescência
Limites: Animais
Masculino
Ratos
Responsável: BR342.1 - Biblioteca Universitária de Saúde


  8 / 2551 LILACS  
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Texto completo SciELO Chile
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Id: biblio-844405
Autor: Flores, Juan Carlos; Bohmwald, Karen; Espinoza, Janyra; Jara, Crlstlna; Peña, Marcela; Hoyos-Bachiloglu, Rodrigo; Iturriaga, Carolina; Kalergis, Alexis M; Borzutzky, Arturo.
Título: Potenciales consecuencias neurocognitivas de infección por virus respiratorio sincicial humano / Potential neurocognitive consequences of infection by human respiratory syncytial virus
Fonte: Rev. chil. infectol;33(5):537-542, oct. 2016. ilus.
Idioma: es.
Projeto: Pontificia Universidad Católica de Chile. Vicerectoría de Investigación; . Instituto Milenio en Inmunología e Inmunoterapia; . Fondo de Desarrollo Científico y Tecnológico; . CONICYT.
Resumo: Human respiratory syncytial virus (RSV) infection remains as a major cause of morbidity and mortality among pediatric population. Immune response is poor and unable to establish a long term effective protection against this virus. Of particular interest has been the description of extrapulmonary manifestations of RSV infection in liver, kidney, endocrine system, heart and brain, associated to infection of peripheral blood. In the central nervous system (CNS), recent studies in animals have suggested long term neurocognitive impairment due to a direct damage from the virus. This was prevented in rats by a recombinant BCG vaccine expressing a nucleoprotein N of RSV that produces an effective immune response against the virus, not allowing its dissemination to the CNS. These findings in animal models highlight the importance of conducting more specific studies in children affected with severe infection by RSV. Therefore, our group is currently conducting an assessment of the possible long-term cognitive impairment in children under 2 years. The results of this study could be a strong argument to continue looking for an effective method for protecting against RSV infection.

La infección por virus respiratorio sincicial humano (VRS) es una de las principales causas de morbimortalidad en población pediátrica. La respuesta inmune generada contra VRS es poco eficiente para su eliminación y logra establecer sólo protección parcial contra infecciones posteriores. De especial interés en los últimos años ha sido la descripción de manifestaciones extra-pulmonares de la infección por VRS en hígado, riñón, sistema endocrino, corazón y cerebro. A nivel de sistema nervioso central (SNC), estudios recientes en modelos animales han sugerido problemas neurocognitivos a largo plazo derivados de un daño directo del virus en el cerebro. Este daño logró ser prevenido con vacuna experimental BCG recombinante, que expresa la nucleoproteína N de VRS e induce inmunidad efectiva, impidiendo la diseminación del virus hacia el SNC. Estos hallazgos en modelo animal han dado cuenta de la importancia de efectuar estudios más detallados en niños afectados por VRS grave. Por tal motivo, actualmente se está realizando una evaluación de la posible alteración cognitiva a largo plazo en niños bajo dos años de edad por parte de nuestro grupo. Los resultados de este estudio podrían significar un argumento muy importante para continuar en la búsqueda de un método efectivo de protección contra esta infección.
Descritores: Infecções por Vírus Respiratório Sincicial/complicações
Viroses do Sistema Nervoso Central/virologia
-Índice de Gravidade de Doença
Doença Aguda
Modelos Animais de Doenças
Limites: Humanos
Animais
Ratos
Tipo de Publ: Revisão
Responsável: CL1.1 - Biblioteca Central


  9 / 2551 LILACS  
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Ambrosano, Gláucia Maria Bovi
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Id: biblio-1001598
Autor: Pauli, Maria Cibelle; Machado, Cínthia Pereira; Silva, Silas Arandas Monteiro e; Ambrosano, Gláucia Maria Bovi; Lopez, Renata Fonseca Vianna; Leonardi, Gislaine Ricci.
Título: Effect of iontophoresis on fluoride uptake in enamel with artificial caries lesion
Fonte: Braz. oral res. (Online);33:e037, 2019. tab, graf.
Idioma: en.
Projeto: CAPES; . FAPESP.
Resumo: Abstract: Iontophoresis is a noninvasive technique, based on the application of a constant low-intensity electric current to facilitate the release of a variety of drugs, whether ionized or not, through biological membranes. The objective of this research was to evaluate the effect of iontophoresis using different electric current intensities on the uptake of fluoride in dental enamel with artificial caries lesions. In this in vitro operator-blind experiment, bovine enamel blocks (n = 10/group) with caries-like lesions and predetermined surface hardness were randomized into 6 groups: placebo gel without fluoride applied with a current of 0.8 mA (negative control), 2% NaF gel without application of any current, and 2% NaF gel applied with currents of 0.1, 0.2, 0.4 and 0.8 mA. Cathodic iontophoresis was applied for 4 min. The concentration of loosely bound fluoride (calcium fluoride) and firmly bound fluoride (fluorapatite) was determined. The results were analyzed by the nonparametric Kruskal-Wallis and Dunn tests. Iontophoresis at 0.8 mA, combined with the application of fluoridated gel (2% NaF), increased fluoride uptake in enamel with caries-like lesions, as either calcium fluoride or fluorapatite.
Descritores: Cariostáticos/farmacologia
Iontoforese/métodos
Cárie Dentária
Esmalte Dentário/efeitos dos fármacos
Fluoretos/farmacologia
-Apatitas/análise
Propriedades de Superfície/efeitos dos fármacos
Fluoreto de Cálcio/análise
Distribuição Aleatória
Resultado do Tratamento
Modelos Animais de Doenças
Eletricidade
Dureza/efeitos dos fármacos
Limites: Animais
Bovinos
Responsável: BR1.1 - BIREME


  10 / 2551 LILACS  
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Matsumoto, Mariza Akemi
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Id: biblio-1011654
Autor: Cardoso, Camila Lopes; Curra, Cláudia; Curi, Marcos Martins; Matsumoto, Mariza Akemi; Argentino, Camila Dionísio; Franzolin, Solange de Oliveira Braga; Constantino, Dulce; Barbosa, Daniela Nicolielo; Ferreira Júnior, Osny.
Título: Treatment of bisphosphonate-related osteonecrosis using platelet-rich plasma: microtomographic, microscopic, and immunohistochemical analyses
Fonte: Braz. oral res. (Online);33:e050, 2019. tab, graf.
Idioma: en.
Projeto: São Paulo Research Foundation.
Resumo: Abstract The present study aimed to investigate the use of platelet-rich plasma (PRP) on tooth extraction sites in rats treated with bisphosphonates. Thirty Albinus Wistar male rats were administered 0.035 mg/kg zoledronic acid intravenously for 8 weeks, divided into four administrations with a 2-week interval between each application, after which their upper right central incisors were extracted to induce the development of bisphosphonate-related osteonecrosis of the jaw (BRONJ). The samples were divided into the following two groups: Group 1 (G1) underwent marginal resection of BRONJ followed by the use of PRP, while Group 2 (G2) underwent resection of BRONJ but without the use of PRP. The treatment groups were evaluated after 14, 28, and 42 days. Clinical, microtomographic, microscopic, and immunohistochemical (IHC) evaluations were performed. Microtomography results revealed no significant difference between the groups (p <0.05) in any time period. Histomorphometric analysis showed increased bone formation over time for both groups (p < 0.001). G1 demonstrated a greater amount of new bone formation than G2 at 28 and 42 days (p < 0.001), with G1 presenting greater vascularization and a slightly higher VEGF expression. For both groups, RANKL/OPG expression levels were sufficient as a parameter for indicating the rate of bone remodeling in a previously treated area of osteonecrosis groups. Taken together, our findings indicated that the use of PRP improves the resolution process of BRONJ.
Descritores: Difosfonatos/uso terapêutico
Conservadores da Densidade Óssea/uso terapêutico
Plasma Rico em Plaquetas
Osteonecrose da Arcada Osseodentária Associada a Difosfonatos/terapia
-Osteoclastos/efeitos dos fármacos
Extração Dentária/efeitos adversos
Cicatrização
Ratos Wistar
Modelos Animais de Doenças
Osteonecrose da Arcada Osseodentária Associada a Difosfonatos/fisiopatologia
Osteonecrose da Arcada Osseodentária Associada a Difosfonatos/patologia
Limites: Animais
Masculino
Ratos
Responsável: BR1.1 - BIREME



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BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde