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Id: biblio-989483
Autor: Degrazia, Felipe Weidenbach; Altmann, Aline Segatto Pires; Ferreira, Carolina Jung; Arthur, Rodrigo Alex; Leitune, Vicente Castelo Branco; Samuel, Susana Maria Werner; Collares, Fabrício Mezzomo.
Título: Evaluation of an antibacterial orthodontic adhesive incorporated with niobium-based bioglass: an in situ study
Fonte: Braz. oral res. (Online);33:e010, 2019. tab, graf.
Idioma: en.
Resumo: Abstract This in situ study aimed to evaluate the antibacterial and anti-demineralization effects of an experimental orthodontic adhesive containing triazine and niobium phosphate bioglass (TAT) around brackets bonded to enamel surfaces. Sixteen volunteers were selected to use intra-oral devices with six metallic brackets bonded to enamel blocks. The experimental orthodontic adhesives were composed by 75% BisGMA and 25% TEGDMA containing 0% TAT and 20% TAT. Transbond XT adhesive (TXT) was used as a control group. Ten volunteers, mean age of 29 years, were included in the study. The six blocks of each volunteer were detached from the appliance after 7 and 14 days to evaluate mineral loss and bacterial growth including total bacteria, total Streptococci, Streptococci mutans, and Lactobacilli. Statistical analysis was performed using GLM model - univariate analysis of variance for microhardness and 2-way ANOVA for bacterial growth (p<0.05). The 20% TAT adhesive caused no difference between distances from bracket and the sound zone at 10-µm deep after 7 and 14 days. After 14 days, higher mineral loss was shown around brackets at 10- to 30-µm deep for TXT and 0% TAT adhesives compared to 20% TAT. S. mutans growth was inhibited by 20% TAT adhesive at 14 days. Adhesive with 20% TAT showed lower S. mutans and total Streptococci growth than 0% TAT and TXT adhesives. The findings of this study show that the adhesive incorporated by triazine and niobium phosphate bioglass had an anti-demineralization effect while inhibiting S. mutans and total Streptococci growth. The use of this product may inhibit mineral loss of enamel, preventing the formation of white spot lesions.
Descritores: Óxidos/farmacologia
Fosfatos/farmacologia
Streptococcus/efeitos dos fármacos
Desmineralização do Dente/prevenção & controle
Cimentos Dentários/farmacologia
Lactobacillus/efeitos dos fármacos
Antibacterianos/farmacologia
Nióbio/farmacologia
-Cerâmica/farmacologia
Cerâmica/química
Método Duplo-Cego
Cimentos Dentários/química
Antibacterianos/química
Limites: Humanos
Masculino
Feminino
Adulto
Adulto Jovem
Responsável: BR1.1 - BIREME


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Id: biblio-989478
Autor: Onwubu, Stanley Chibuzor; Mdluli, Phumlane Selby; Singh, Shenuka; Tlapana, Tshepo.
Título: A novel application of nano eggshell/titanium dioxide composite on occluding dentine tubules: an in vitro study
Fonte: Braz. oral res. (Online);33:e016, 2019. tab, graf.
Idioma: en.
Projeto: National Research Foundation of South Africa.
Resumo: Abstract To synthesize Nano eggshell-titanium-dioxide (EB@TiO2) biocomposite and to evaluate its effectiveness in occluding opened dentine tubules. EB@TiO2 was synthesized and characterized using X-ray diffraction (XRD), and Transmission Electron Microscope (TEM). Sixteen simulated bovine dentine discs were prepared and randomly assigned into four groups according to the following treatment (n = 4): Group 1: No treatment; Group 2: eggshell powder; Group 3: EB@TiO2; Group 4: Sensodyne. These were then agitated in a solution of 1g powder and 40mL water for 3hours. Thereafter, each dentine discs from the respective groups were post-treated for 5 min with 2wt% citric acid to test their acid resistant characteristics. Scanning Electron Microscope (SEM) was used to observe the effectiveness of occluded dentine pre-and post-treatment. The cytotoxicity of the synthesized EB@TiO2 was tested using NIH 3T3 assay. ANOVA was used to evaluate the mean values of the occluded area ratio and the data of MTS assay. This was followed by a multi-comparison test with Bonferroni correction (α = .05). The XRD confirmed that EB@TiO2 was successfully modified through ball-milling. The TEM revealed the presence of both spherical and irregular particle shape powders. The SEM result showed that EB@TiO2 could effectively occlude open dentine tubules. Equally, the result demonstrated that EB@TiO2 exhibited the highest acid resistant stability post-treatment. NIH 3T3 assay identified that EB@TiO2 had little effect on the NIH 3T3 cell line even at the highest concentration of 100µg/ml. This study suggests that the application of EB@TiO2 effectively occluded dentine tubules and the occlusion showed a high acid resistant stability.
Descritores: Fosfatos/farmacologia
Titânio/química
Permeabilidade da Dentina/efeitos dos fármacos
Sensibilidade da Dentina/terapia
Casca de Ovo/química
Nanocompostos/química
Dessensibilizantes Dentinários/farmacologia
Fluoretos/farmacologia
Nitratos/farmacologia
-Titânio/análise
Titânio/farmacologia
Remineralização Dentária
Microscopia Eletrônica de Varredura
Células NIH 3T3
Combinação de Medicamentos
Casca de Ovo/ultraestrutura
Nanocompostos/análise
Nanocompostos/uso terapêutico
Limites: Animais
Bovinos
Camundongos
Responsável: BR1.1 - BIREME


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Id: biblio-1045625
Autor: Leenders, TJM; Eijndhoven, FHA van; Veer, E van der; Muskiet, FAJ.
Título: Vitamin D deficiency in risk groups living in tropical curaçao / Deficiencia de vitamina D en los grupos de riesgo de curazao tropical
Fonte: West Indian med. j;62(3):195-200, Mar. 2013. ilus, tab.
Idioma: en.
Resumo: OBJECTIVE: Curaçao (12 degrees 10N, 69 degrees 0W) is characterized by whole year abundant sunshine (8-10 hours/day). We challenged the automatic assumption that people living in tropical countries do not have a high risk of vitamin D deficiency, and investigated the vitamin D status in a tropical environment. METHODS: For this, we selected fiftytwo elderly people with little or no exposure to direct sunlight [median 84 (60-96) years; 34 females, 18 males] and who were cared for by community nurses or lived in retirement or nursing homes. Furthermore, six rehabilitating orthopaedic patients [median 72 (38-90) years; one female, five males] were included. Serum 25(OH)D, calcium, phosphate, parathyroid hormone (PTH) and creatinine were measured. Those exhibiting elevated creatinine, PTH or both had their 1,25dihydroxyvitamin D [1,25(OH)2D] examined. RESULTS: Serum levels of 25(OH)D below 25, 50 and 75 nmol/L were detected in, respectively, seven (12%), 22 (38%) and 48 (83%) of the fiftyeight persons. Four persons had combined high creatinine and PTH, and low 1,25(OH)2D, which was not known by their caregivers. CONCLUSION: Abundant sunshine outdoors is no guarantee for vitamin D sufficiency. More attention is needed for vitamin D deficiency in risk groups living in tropical areas and elderly persons with poor kidney function.

OBJETIVO: Curazao (12 grados 10N, 69 grados 0W) se caracteriza por su abundante luz solar durante todo el año (8-10 horas/día). Ponemos en duda la suposición automática de que los habitantes de países tropicales no poseen un alto riesgo de deficiencia de vitamina D, e investigamos los niveles de vitamina D en un ambiente tropical. MÉTODOS: Para ello, seleccionamos a cincuenta y dos personas de avanzada edad [mediana 84 (60-96) años; 34 mujeres, 18 hombres] con poca o ninguna exposición directa a la luz solar, cuidados por enfermeras comunitarias o que vivían en hogares de ancianos o casas de retiro. Además, se incluyeron seis pacientes bajo rehabilitación ortopédica [mediana 72 (38-90) años; una mujer, cinco hombres]. Se les realizaron las siguientes mediciones: 25(OH)D en suero, calcio, fosfato, hormona paratiroidea (HPT), y creatinina. A todos aquellos que mostraron niveles elevados de creatinina, de HPT, o de ambas, se les realizó un examen de 1,25dihidroxivitamina D [1,25(OH)2D]. RESULTADOS: Se detectaron niveles séricos de 25(OH)D por debajo de 25, 50 y 75 nmol/L en siete (12%), 22 (38%) y 48 (83%) de las cincuenta y ocho personas, respectivamente. Cuatro personas presentaron una combinación de elevada creatinina y HPT, y bajo 1,25(OH)2D, datos desconocidos para sus cuidadores. CONCLUSIÓN: La abundancia de luz solar exterior no es garantía de suficiencia de vitamina D. Se necesita prestar mayor atención a la deficiencia de vitamina D en los grupos de riesgo que viven en áreas tropicales, así como a las personas de edad avanzada con pobre función renal.
Descritores: Luz Solar
Deficiência de Vitamina D/epidemiologia
Grupos de Risco
-Hormônio Paratireóideo/sangue
Fosfatos/sangue
Vitamina D/sangue
Deficiência de Vitamina D/sangue
Cálcio/sangue
Creatinina/sangue
Antilhas Holandesas/epidemiologia
Limites: Humanos
Masculino
Feminino
Pessoa de Meia-Idade
Idoso
Idoso de 80 Anos ou mais
Responsável: BR1.1 - BIREME


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Id: biblio-1019608
Autor: Almeida, Amanda Bandeira de; Santos, Elis Janaína Lira dos; Abuna, Gabriel Flores; Ribeiro, Cristiane Salmon; Casati, Márcio Zaffalon; Ruiz, Karina Gonzales Silvério; Nociti Júnior, Francisco Humberto.
Título: Isolation and characterization of a human cementocyte-like cell line, HCY-23
Fonte: Braz. oral res. (Online);33:e058, 2019. tab, graf.
Idioma: en.
Projeto: São Paulo State Research Foundation; . São Paulo State Research Foundation; . National Council for Scientific and Technological Development.
Resumo: Abstract Cementum is the mineralized tissue covering the tooth root that functions in tooth attachment and post-eruptive adjustment of tooth position. It has been reported to be highly similar to bone in several respects but remains poorly understood in terms of development and regeneration. Here, we investigate whether cementocytes, the residing cells in cellular cementum, have the potential to be protagonist in cementum homeostasis, responding to endocrine signals and directing local cementum metabolism. Cells from healthy erupted human teeth were isolated using sequential collagenase/EDTA digestions, and maintained in standard cell culture conditions. A cementocyte-like cell line was cloned (HCY-23, for human cementocyte clone 23), which presented a cementocyte compatible gene expression signature, including the expression of dentin matrix protein 1 ( DMP1 ), sclerostin ( SOST ), and E11/gp38/podoplanin ( E11 ). In contrast, these cells did not express the odontoblast/dentin marker dentin sialoprotein ( DSPP ). HCY-23 cells produced mineral-like nodules in vitro under differentiation conditions, and were highly responsive to inorganic phosphate (Pi). Within the limits of the present study, it can be concluded that cementocytes are phosphate-responsive cells, and have the potential do play a key role in periodontal homeostasis and regeneration.
Descritores: Marcadores Genéticos/genética
Técnicas de Cultura de Células/métodos
Cemento Dentário/citologia
-Fosfatos/farmacologia
Fosfoproteínas/análise
Fosfoproteínas/genética
Sialoglicoproteínas/análise
Sialoglicoproteínas/genética
Fatores de Tempo
Glicoproteínas de Membrana/análise
Glicoproteínas de Membrana/genética
Expressão Gênica
Linhagem Celular
Análise de Variância
Proteínas da Matriz Extracelular/análise
Proteínas da Matriz Extracelular/genética
Imunofluorescência
Proteínas Morfogenéticas Ósseas/análise
Proteínas Morfogenéticas Ósseas/genética
Cemento Dentário/metabolismo
Proteínas Adaptadoras de Transdução de Sinal
Dente Molar/citologia
Limites: Humanos
Masculino
Feminino
Adolescente
Adulto
Adulto Jovem
Responsável: BR1.1 - BIREME


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Id: biblio-1291888
Autor: Brance, María Lorena; Larroudé, María Silvia; Berbotto, Guillermo; Sacnun, Mónica P; Aeschlimann, Carolina; Palatnik, Mariano; Chavero, Ignacio; Sánchez, Ariel; Brun, Lucas R.
Título: Negative association between 25-hydroxyvitamin D and inflammatory markers in rheumatologic diseases / Asociación negativa entre 25-hidroxi vitamina D y los marcadores inflamatorios en enfermedades reumatológicas
Fonte: Actual. osteol;17(1):8-17, 2021. graf, tab.
Idioma: en.
Resumo: Objective: The main purpose of this study was to evaluate serum 25-hydroxyvitamin D (25OHD) levels and its association with in"ammatory markers in patients with rheumatologic diseases (RD). Methods: A cross-sectional study in 154 women with RD (rheumatoid arthritis, spondyloarthritis and other connective tissue diseases) and 112 healthy individuals as a control group (CG) was carried out. Results: No differences in serum and urine calcium, serum phosphate, and urinary deoxypyridinoline were found. RD group had lower 25OHD and higher PTH compared to CG. RD group had higher C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR) compared to CG. The overall mean level of 25OHD (ng/ml) was 26.3±12.0 in the CG and 19.4±6.8 in the RD group (p<0.0001). Moreover, CG had lower percentage of individuals with 25OHD de!ciency compared to RD (29.9% vs 53.2%). The femoral neck BMD was signi!cantly lower in postmenopausal RD women compared to CG. 25OHD levels signi!cantly correlated with ESR and CRP as in"ammatory markers. Age, BMI, presence of RD, and CRP were signi!cantly and negatively associated with 25OHD levels through linear regression analysis. According to univariate logistic regression analysis for 25OHD deficiency (<20 ng/ml), a significant and negative association with BMI, presence of RD, ESR and CRP were found. Conclusion: Patients with RD had lower 25OHD levels than controls and the presence of a RD increases by 2.66 the risk of vitamin D de!ciency. In addition, 25OHD has a negative correlation with ESR and CRP as in"ammatory markers. (AU)

Objetivo El objetivo principal de este estudio fue evaluar los niveles séricos de 25-hidroxivitamina D (25OHD) y su asociación con marcadores inflamatorios en enfermedades reumatológicas. Materiales y métodos: Se realizó un estudio transversal en 154 mujeres con enfermedades reumatológicas (artritis reumatoide, espondiloartritis y otras enfermedades del tejido conectivo) y 112 individuos sanos como grupo control (GC). Resultados: No se encontraron diferencias en el calcio sérico y urinario, el fosfato sérico y la desoxipiridinolina urinaria entre el GC y los sujetos con enfermedades reumatológicas. El grupo de pacientes con enfermedades reumatológicas tenía 25OHD más bajo y PTH más alto en comparación con el GC. Asimismo, el grupo de individuos con enfermedades reumatológicas tenía proteína C reactiva (PCR) y velocidad de eritrosedimentación (VES) más altas en comparación con el GC. El nivel de 25OHD (ng/ml) fue 26,3±12,0 en el GC y 19,4±6,8 en el grupo con enfermedades reumatológicas (p<0,0001). Además, el GC presentó un porcentaje menor de deficiencia de 25OHD en comparación con el grupo con enfermedades reumatológicas (29,9% vs 53,2%). La DMO del cuello femoral fue significativamente menor en las mujeres posmenopáusicas con enfermedades reumatológicas en comparación con el GC. La 25OHD correlacionó significativamente con la VES y la PCR como marcadores inflamatorios. El análisis de regresión lineal mostró que la edad, el IMC, la presencia de una enfermedad reumatológica y la PCR se asociaron significativa y negativamente con los niveles de 25OHD. Mientras que el análisis de regresión logística univariada mostró que la deficiencia de 25OHD (<20 ng/ml), se asoció significativa y negativamente con el IMC, la presencia de una enfermedad reumatológica, la VES y los niveles de PCR. Conclusiones: Los pacientes con enfermedades reumatológicas tenían niveles de 25OHD más bajos que los controles y la presencia de una enfermedad reumatológica aumenta en 2.66 el riesgo de deficiencia de vitamina D. Además, la 25OHD mostró correlación negativa con la VES y la PCR como marcadores inflamatorios. (AU)
Descritores: Vitamina D/análogos & derivados
Vitamina D/sangue
Deficiência de Vitamina D/etiologia
Biomarcadores
Doenças Reumáticas/complicações
Inflamação/sangue
-Fosfatos/sangue
Sedimentação Sanguínea
Proteína C-Reativa
Índice de Massa Corporal
Densidade Óssea
Modelos Logísticos
Cálcio/urina
Cálcio/sangue
Doenças Reumáticas/sangue
Risco
Estudos Transversais
Pós-Menopausa
Aminoácidos/urina
Limites: Humanos
Feminino
Adolescente
Adulto
Pessoa de Meia-Idade
Idoso
Idoso de 80 Anos ou mais
Adulto Jovem
Tipo de Publ: Estudo Comparativo
Responsável: AR2.1 - Biblioteca Central


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Id: biblio-1254838
Autor: Al-Aizari, Ahmed Ali; Al-Obeed, Rashid S; Mohamed, Mahmoud AH.
Título: Improving micropropagation of some grape cultivars via boron, calcium and phosphate
Fonte: Electron. j. biotechnol;48:95-100, nov. 2020. ilus, tab.
Idioma: en.
Resumo: BACKGROUND: An efficient regeneration protocol is a priority for the successful application of plant biotechnology. Grape nodal explants were used to develop a micropropagation protocol for Thompson Seedless and Taify cvs. Explants were cultured on MS medium supplemented with Kinetin or benzylaminopurine (BA) and indolebutyric acid (IBA). RESULTS: For both cultivars, axillary buds were grown, only, on a medium enriched with kinetin, moreover, shoot tip necrosis and callus formation were observed on Thompson Seedless cv. cultures grown on a medium with BA. Supplementing the growth medium with 100 mM (boron) B and 2.5 mM (calcium) Ca successfully help overcome these phenomena. The highest regenerated shoot numbers (14 and 6.2 explant 1 ) for Taify and Thompson Seedless cvs., respectively, were on media supplemented with 13.2 mM BA + 4.9 mM IBA and BA 13.2 mM + 5.8 mM IBA, respectively. Moreover, these media supported the developing shoots to have the heaviest dry weights (1.46 and 0.72 mg explant 1 ) for Taify and Thompson Seedless cvs., respectively. Thompson Seedless cv. regenerated shoot numbers and their dry weights were significantly increased by increasing the MS medium PO4 concentration. However, these two parameters were significantly decreased for Taify cv. Developing shoots were elongated and rooted on MS medium enriched with 4.9 mM, IBA 100 mM B and 2.5 mM Ca. Plantlets were acclimatized and successfully transferred to the greenhouse conditions. CONCLUSIONS: A novel promising protocol for Thomson Seedless and Taify cvs. micropropagation using single nodes has been developed.
Descritores: Fosfatos/química
Boro/química
Cálcio/química
Vitis/crescimento & desenvolvimento
-Regeneração
Biotecnologia
Brotos de Planta
Necrose/prevenção & controle
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Brasil
Cury, Jaime Aparecido
Tenuta, Lívia Maria Andaló
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Id: biblio-962177
Autor: Cury, Jaime Aparecido; Caldarelli, Pablo Guilherme; Tenuta, Livia Maria Andaló.
Título: Necessity to review the Brazilian regulation about fluoride toothpastes / Necessidade de revisão da regulamentação brasileira sobre dentifrícios fluoretados
Fonte: Rev. saúde pública (Online);49:74, 2015. tab.
Idioma: en.
Resumo: The aim of this study was to evaluate the adequacy of the Brazilian legislation about fluoride toothpaste. A search was conducted in LILACS, Medline and SciELO databases about the fluoride concentration found in Brazilians toothpastes, using descriptors on health. Publications since 1981 have shown that some Brazilian toothpastes are not able to maintain, during their expiration time, a minimum of 1,000 ppm F of soluble fluoride in the formulation. However, the Brazilian regulation (ANVISA, Resolution 79, August 28, 2000) only sets the maximum total fluoride (0.15%; 1,500 ppm F) that a toothpaste may contain but not the minimum concentration of soluble fluoride that it should contain to have anticaries potential, which according to systematic reviews should be 1,000 ppm F. Therefore, the Brazilian regulation on fluoride toothpastes needs to be revised to assure the efficacy of those products for caries control.

O presente estudo analisou a adequação da regulamentação brasileira vigente sobre dentifrícios fluoretados. Foi realizada busca da literatura sobre a concentração de flúor nos dentifrícios brasileiros, tendo como fontes Lilacs, Medline e SciELO, com uso de descritores em saúde. Publicações sobre a composição de dentifrícios fluoretados brasileiros têm mostrado desde 1981 que nem todos os dentifrícios são capazes de manter durante o prazo de validade uma concentração mínima de 1.000 ppm F. Esse problema ocorre não só com dentifrícios adquiridos no mercado como com aqueles distribuídos por serviços públicos de saúde. Entretanto, a legislação brasileira (Resolução 79, Anvisa, 28/8/2000) apenas estabelece que um dentifrício não pode conter mais que 0,15% (1.500 ppm F) de flúor total, sem estabelecer o mínimo de flúor solúvel que ele deveria conter para ter potencial anticárie, a qual de acordo com revisões sistemáticas deve ser igual ou maior que 1.000 ppm F. Concluiu-se que a regulamentação brasileira sobre dentifrícios fluoretados necessita ser revisada, garantindo a eficácia desses produtos para o controle da cárie.
Descritores: Cremes Dentais/análise
Cariostáticos/análise
Fluoretação/legislação & jurisprudência
Cárie Dentária/prevenção & controle
-Fosfatos/análise
Fluoreto de Sódio/análise
Brasil
Fluoretos/análise
Limites: Humanos
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME


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Texto completo SciELO Chile
Belangero, William Dias
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Id: biblio-990013
Autor: Caetano, Heliard Rodrigues dos Santos; Castoldi, Robson Chacon; Zanuto, Éverton Alex Carvalho; Ozaki, Guilherme Akio Tamura; Garcia, Thiago Alves; Pereira, João Domingos Augusto dos Santos; Constantino, Carlos José Leopoldo; Bremer-Neto, Hermann; Giometti, Ines Cristina; Camargo, Regina Celi Trindade; Camargo-Filho, José Carlos Silva; Belangero, William Dias.
Título: Muscle strength training is better than the use of growth hormone (GH) in bone health of Wistar rats / Entrenamiento de la fuerza muscular es mejor que el uso de la hormona del crecimiento (GH) en salud ósea del ratas Wistar
Fonte: Int. j. morphol;37(1):104-110, 2019. graf.
Idioma: en.
Resumo: SUMMARY: The objective of this study was to evaluate the effects of growth hormone (GH) and muscle strength training (ST) on the composition of bone tissue of Wistar rats through Raman spectroscopy. In total, 40 male rats were randomly distributed into four groups: (N = 10) control (C), control with the application of GH (GHC), strength training (T), and strength training with the application of GH (GHT). The training consisted of four series of 10 water jumps, performed three times a week, with an overload corresponding to 50 % of body weight and duration of four weeks. GH was applied at a dose of 0.2 IU / kg in each animal three times a week and every other day. After four weeks, the animals were euthanized and the right femurs collected for analysis of the bone structure. Raman spectroscopy (ER) was used to observe the following compounds from their respective bands: Calcium Carbonate-Triglycerides (fatty acids) 1073 cm-1, Collagen type I 509 cm-1, Bone-DNA Phosphate (Protein) 589 cm-1, Phosphate Phospholipids 1078 cm-1. For the statistical analysis, the Shapiro-Wilk and ANOVA One-Way variance analysis normality tests were performed, followed by the Tukey post-test. The results showed an increase in the concentrations of calcium carbonate-triglycerides (fatty acids), type I collagen, bone phosphate-DNA (protein), and phosphate phospholipids in all experimental groups, with or without ST and/or GH , But only the isolated training group differed significantly from the control group (P <0.05). It was concluded that all treatments could promote bone tissue gain, however, only the T group demonstrated a significant difference in the mineral compounds analyzed.

RESUMEN: El objetivo del estudio fue avaluar el efecto de la aplicación de la hormona del crecimiento (GH) y entrenamiento de la fuerza muscular (EF) en la composición del tejido óseo de ratas Wistar a partir de la espectroscopía Raman. Fueron utilizadas 40 ratas machos distribuidas de forma aleatoria en cuatro grupos (n=10): control (C), control y aplicación de GH (GHC), entrenamiento de la fuerza muscular (EF) y entrenamiento de la fuerza muscular y aplicación del GH (GHE). El entrenamiento fue consistió en cuatro series de 10 saltos acuáticos, realizados tres veces en la semana, con sobrecarga correspondiente a 50 % de la masa corporal y durante cuatro semanas. El GH fue aplicado en la dosificación de 0,2 UI/kg en cada animal, tres veces en la semana y en días alternados. Después de cuatro semanas, los animales fueran eutanasiados y retirados los fémures derechos para un análisis de la estructura ósea. La espectroscopía Raman fue utilizada para observar los siguientes compuestos a partir de las respectivas bandas: Carbonato de Calcio-Triglicéridos (ácidos grasos) 1073 cm-1, Colágeno Tipo I 509 cm-1, Fosfato Óseo-DNA (Proteína) 589 cm1, Fosfato Fosfolípidos 1078 cm-1. Para el análisis estadístico, fueron realizadas las pruebas Shapiro-Wilk y el análisis de variancia ANOVA One-Way, seguida de test post hoc de Tukey. Los resultados revelaran aumento de la concentración de Carbonato de Calcio-Triglicéridos (ácidos grasos), Colágeno Tipo I, Fosfato Óseo- DNA (Proteína), Fosfato Fosfolípidos en todos los grupos experimentales, asociados o no a la realización del EF y/o aplicación del GH. Además, solamente el grupo EF mostró diferencia significativa del grupo C (p<0,05). Es posible concluir que todos los tratamientos mostraran aumentos en el tejido óseo, sin embargo, solamente el grupo T demostró una diferencia significativa en los compuestos minerales analizados.
Descritores: Natação/fisiologia
Osso e Ossos/química
Força Muscular/fisiologia
-Fosfatos/análise
Análise Espectral Raman
Peso Corporal
Osso e Ossos/efeitos dos fármacos
Carbonato de Cálcio/análise
Hormônio do Crescimento/administração & dosagem
Exercício Físico/fisiologia
Densidade Óssea
Análise de Variância
Colágeno/análise
Ratos Wistar
Limites: Animais
Masculino
Ratos
Responsável: CL1.1 - Biblioteca Central


  9 / 250 LILACS  
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Id: biblio-1132178
Autor: Schardong, Roberta Moriconi Freire; Moro, Marcelo Freire; Bonilla, Oriel Herrera.
Título: Aquaponic System with White Shrimp Litopenaeus vannamei Rearing and Production of the Plants Batis maritima, Sarcocornia neei and Sporobolus virginicus
Fonte: Braz. arch. biol. technol;63:e20190118, 2020. tab, graf.
Idioma: en.
Resumo: Abstract Aquaponics is a system that integrates aquaculture with plant production in which two species are benefited, and there is water saving. In this study was carried out with an aquaponic system to verify the interaction between the growth of the halophytes Batis maritime, Sarcocornia neei, and Sporobolus virginicus associated with white shrimp Litopenaeus vannamei rearing. We also investigated if there were different responses of physicochemical variables of the water in the experimental shrimp culture ponds integrated into the growth of plants and control ponds, without plants, throughout a 56-day period. The treatment experiment and control presented a statistically significant difference in total dissolved solids, salinity, total suspended solids, ammonia, orthophosphate, and nitrite. In the experimental treatment, with the presence of plants and recirculating water, a reduction of total suspended solids, ammonia and orthophosphate was observed. The rate of shrimp production was not significantly different between treatments, and the performance was similar to that of other studies. The biomass gain of the halophyte B. maritima was 876.6 grams in 0.5 m² and of S. neei was 48.8 grams in 0.16 m². All plants of the species S. virginicus died during the experiment.
Descritores: Água/química
Chenopodiaceae/crescimento & desenvolvimento
Penaeidae/crescimento & desenvolvimento
Plantas Tolerantes a Sal/crescimento & desenvolvimento
-Fosfatos
Aquicultura
Hidroponia
Biomassa
Chenopodiaceae/metabolismo
Amônia
Nitritos
Limites: Animais
Responsável: BR1.1 - BIREME


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Id: biblio-1142513
Autor: Guera, Keli Cristina Silva; Fonseca, Adriel Ferreira da; Ribeiro, Fernanda.
Título: Stocks and Distribution of Soil Carbon, Nitrogen, Phosphorus and Sulfur in an Integrated Crop-Livestock System Treated with Phosphates
Fonte: Braz. arch. biol. technol;63(spe):e20190520, 2020. tab, graf.
Idioma: en.
Projeto: National Council for Scientific and Technological Development; . Araucaria Foundation.
Resumo: Abstract Conservation agriculture practices can contribute to changes in soil nutrient dynamics over time. This experiment evaluated the changes in total stocks and distribution of carbon, nitrogen, phosphorus and sulfur concentrations in soil, during 60 months, in an integrated crop-livestock system (ICLS) due to anticipated fertilization of sources and doses phosphates applied in soil surface. The experiment was conducted over a period of five years, under Typic Dystrudept, using a randomized block design, in an incomplete factorial scheme (3×3+1), with four replications. Treatments consisted of three sources of P [triple superphosphate (TSP), rock phosphate - Arad (RP) and magnesium thermophosphate (MTP)], along with four doses of P (0, 60, 120 and 180 kg ha-1 P2O5 total). Samples of soil were collected in 0-5, 5-10, 10-15, 15-20 and 20-30 cm layers at 24, 36, 48 and 60 months after beggining of experiment where the following chemical attributes were evaluated: (i) total organic carbon (TOC); (ii) total nitrogen Kjeldahl (TNK); (iii) available P by ion exchange resin method (P-IER); and (iv) available S-SO4 2-. The ICLS conditions provided increased total stocks and concentrations of TOC, TNK, P-IER and S-SO4 2- over time. The applications of different phosphates had no influence on soil TOC concentrations during the five years of experimentation. The concentrations of TNK, P-IER and S-SO4 2- showed an increase in different layers of soil, with the application of sources and doses of P. The P fertilization practice that was anticipated can consist of an efficient management of soil fertility, using properly managed conservation systems.
Descritores: Fosfatos/administração & dosagem
Solo/química
Cultivos Agrícolas
Análise do Solo
Fertilizantes
Criação de Animais Domésticos
-Fósforo/análise
Enxofre/análise
Carbono/análise
Nitrogênio/análise
Limites: Humanos
Animais
Responsável: BR1.1 - BIREME



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