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Id: biblio-950772
Autor: Zeb, Anwar; Sadiq, Abdul; Ullah, Farhat; Ahmad, Sajjad; Ayaz, Muhammad.
Título: Investigations of anticholinestrase and antioxidant potentials of methanolic extract, subsequent fractions, crude saponins and flavonoids isolated from Isodon rugosus
Fonte: Biol. Res;47:1-10, 2014. graf, tab.
Idioma: en.
Resumo: BACKGROUND: Based on the ethnomedicinal uses and the effective outcomes of natural products in various diseases, this study was designed to evaluate Isodon rugosus as possible remedy in oxidative stress, alzheimer's and other neurodegenerative diseases. Acetylecholinestrase (AChE) and butyrylcholinesterase (BChE) inhibitory activities of crude methanolic extract (Ir.Cr), resultant fractions (n-hexane (Ir.Hex), chloroform (Ir.Cf), ethyl acetate (Ir.EtAc), aqueous (Ir.Aq)), flavonoids (Ir.Flv) and crude saponins (Ir.Sp) of I. rugosus were investigated using Ellman's spectrophotometric method. Antioxidant potential of I. rugosus was determined using DPPH, H2O2 and ABTS free radicals scavenging assays. Total phenolic and flavonoids contents of plant extracts were determined and expressed in mg GAE/g dry weight and mg RTE/g of dry sample respectively. RESULTS: Among different fractions Ir.Flv and Ir.Cf exhibited highest inhibitory activity against AChE (87.44 ± 0.51, 83.73 ± 0.64%) and BChE (82.53 ± 0.71, 88.55 ± 0.77%) enzymes at 1 mg/ml with IC50 values of 45, 50 for AChE and 40, 70 µg/ml for BChE respectively. Activity of these fractions were comparable to galanthamine causing 96.00 ± 0.30 and 88.61 ± 0.43% inhibition of AChE and BChE at 1 mg/ml concentration with IC50 values of 20 and 47 µg/ml respectively. In antioxidant assays, Ir.Flv, Ir.Cf, and Ir.EtAc demonstrated highest radicals scavenging activities in DPPH and H2O2 assays which were comparable to ascorbic acid. Ir.Flv was found most potent with IC50 of 19 and 24 µg/ml against DPPH and H2O2 radicals respectively. Whereas antioxidant activates of plant samples against ABTS free radicals was moderate. Ir.Cf, Ir.EtAc and Ir.Cr showed high phenolic and flavonoid contents and concentrations of these compounds in different fractions correlated well to their antioxidant and anticholinestrase activities. CONCLUSION: It may be inferred from the current investigations that the Ir.Sp, Ir.Flv and various fractions of I. rugosus are good sources of anticholinesterase and antioxidant compounds. Different fractions can be subjected to activity guided isolation of bioactive compounds effective in neurological disorders.
Descritores: Saponinas/análise
Flavonoides/análise
Extratos Vegetais/química
Inibidores da Colinesterase/análise
Isodon/química
Antioxidantes/análise
-Picratos/metabolismo
Acetilcolinesterase/efeitos dos fármacos
Saponinas/isolamento & purificação
Espectrofotometria/métodos
Ácidos Sulfônicos/metabolismo
Flavonoides/isolamento & purificação
Compostos de Bifenilo/metabolismo
Butirilcolinesterase/efeitos dos fármacos
Clorofórmio
Sequestradores de Radicais Livres/metabolismo
Estresse Oxidativo/efeitos dos fármacos
Concentração Inibidora 50
Isodon/classificação
Isodon/enzimologia
Componentes Aéreos da Planta/química
Misturas Complexas
Metanol
Benzotiazóis/metabolismo
Radicais Livres/análise
Hexanos
Peróxido de Hidrogênio/metabolismo
Medicina Tradicional
Acetatos
Responsável: CL1.1 - Biblioteca Central


  2 / 10 LILACS  
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Texto completo SciELO Chile
Texto completo
Id: biblio-950779
Autor: Moukette, Bruno Moukette; Pieme, Constant Anatole; Njimou, Jacques Romain; Biapa, Cabral Prosper Nya; Marco, Bravi; Ngogang, Jeanne Yonkeu.
Título: In vitro antioxidant properties, free radicals scavenging activities of extracts and polyphenol composition of a non-timber forest product used as spice: Monodora myristica
Fonte: Biol. Res;48:1-17, 2015. graf, tab.
Idioma: en.
Resumo: BACKGROUND: Excessive production of free radicals causes direct damage to biological molecules such as DNA, proteins, lipids, carbohydrates leading to tumor development and progression. Natural antioxidant molecules from phytochemicals of plant origin may directly inhibit either their production or limit their propagation or destroy them to protect the system. In the present study, Monodora myristica a non-timber forest product consumed in Cameroon as spice was screened for its free radical scavenging properties, antioxidant and enzymes protective activities. Its phenolic compound profile was also realized by HPLC. RESULTS: This study demonstrated that M. myristica has scavenging properties against DPPH',OH',NO', and ABTS'radicals which vary in a dose depending manner. It also showed an antioxidant potential that was comparable with that of Butylated Hydroxytoluene (BHT) and vitamin C used as standard. The aqueous ethanol extract of M. myristica barks (AEH); showed a significantly higher content in polyphenolic compounds (21.44 ± 0.24 mg caffeic acid/g dried extract) and flavonoid (5.69 ± 0.07 quercetin equivalent mg/g of dried weight) as compared to the other studied extracts. The HPLC analysis of the barks and leaves revealed the presence of several polyphenols. The acids (3,4-OH-benzoic, caffeic, gallic, O- and P- coumaric, syringic, vanillic), alcohols (tyrosol and OH-tyrosol), theobromine, quercetin, rutin, catechine and apigenin were the identified and quantified polyphenols. All the tested extracts demonstrated a high protective potential on the superoxide dismutase (SOD), catalase and peroxidase activities. CONCLUSION: Finally, the different extracts from M. myristica and specifically the aqueous ethanol extract reveal several properties such as higher free radical scavenging properties, significant antioxidant capacities and protective potential effects on liver enzymes.
Descritores: Extratos Vegetais/farmacologia
Sequestradores de Radicais Livres/farmacologia
Especiarias
Annonaceae/química
Polifenóis/química
Antioxidantes/farmacologia
-Peroxidases/efeitos dos fármacos
Picratos/metabolismo
Ácidos Sulfônicos/metabolismo
Superóxido Dismutase/efeitos dos fármacos
Flavonoides/análise
Compostos de Bifenilo/metabolismo
Técnicas In Vitro
Camarões
Extratos Vegetais/química
Catalase/efeitos dos fármacos
Florestas
Cromatografia Líquida de Alta Pressão
Radical Hidroxila/metabolismo
Folhas de Planta/química
Casca de Planta/química
Benzotiazóis/metabolismo
Óxido Nítrico/metabolismo
Responsável: CL1.1 - Biblioteca Central


  3 / 10 LILACS  
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Texto completo SciELO Chile
Texto completo
Id: biblio-950785
Autor: Kamal, Zul; Ullah, Farhat; Ayaz, Muhammad; Sadiq, Abdul; Ahmad, Sajjad; Zeb, Anwar; Hussain, Abid; Imran, Muhammad.
Título: Anticholinesterse and antioxidant investigations of crude extracts, subsequent fractions, saponins and flavonoids of atriplex laciniata L: potential effectiveness in Alzheimer's and other neurological disorders
Fonte: Biol. Res;48:1-11, 2015. graf, tab.
Idioma: en.
Resumo: BACKGROUND: Atriplex laciniata L. was investigated for phenolic, flavonoid contents, antioxidant, anticholinesterase activities, in an attempt to explore its effectiveness in Alzheimer's and other neurological disorders. Plant crude methanolic extract (Al.MeF), subsequent fractions; n-hexane (Al.HxF), chloroform (Al.CfF), ethyl acetate (Al.EaF), aqueous (Al.WtF), Saponins (Al.SPF) and Flavonoids (Al.FLVF) were investigated for DPPH, ABTS and H2O2 free radical scavenging activities. Further these extracts were subjected to acetylcholinesterase (AChE) & butyrylcholinesterase (BChE) inhibitory activities using Ellman's assay. Phenolic and Flavonoid contents were determined and expressed in mg Gallic acid GAE/g and Rutin RTE/g of samples respectively. RESULTS: In DPPH free radicals scavenging assay, Al.FLVF, Al.SPF and Al.MeF showed highest activity causing 89.41 ± 0.55, 83.37 ± 0.34 and 83.37 ± 0.34% inhibition of free radicals respectively at 1 mg/mL concentration. IC50 for these fractions were 33, 83 and 82 µg/mL respectively. Similarly, plant extracts showed high ABTS scavenging potential, i.e. Al.FLVF (90.34 ± 0.55), Al.CfF (83.42 ± 0.57), Al.MeF (81.49 ± 0.60) with IC50 of 30, 190 and 70 µg/ml respectively. further, H2O2 percent scavenging was highly appraised in Al.FLVF (91.29 ±0.53, IC50 75), Al.SPF (85.35 ±0.61, IC50 70) and Al.EaF (83.48 ± 0.67, IC50 270 µg/mL). All fractions exhibited concentration dependent AChE inhibitory activity as; Al.FLVF, 88.31 ± 0.57 (IC50 70 µg/mL), Al.SPF, 84.36 ± 0.64 (IC50 90 µg/mL), Al.MeF, 78.65 ± 0.70 (IC50 280 µg/mL), Al.EaF, 77.45 ± 0.46 (IC50 270 µg/mL) and Al.WtF 72.44 ± 0.58 (IC50 263 µg/mL) at 1 mg/mL. Likewise the percent BChE inhibitory activity was most obvious in Al.FLVF 85.46 ± 0.62 (IC50 100 µg/mL), Al.CfF 83.49 ± 0.46 (IC50 160 µg/mL), Al.MeF 82.68 ± 0.60 (IC50 220 µg/mL) and Al.SPF 80.37 ± 0.54 (IC50 120 µg/mL). CONCLUSIONS: These results stipulate that A. laciniata is enriched with phenolic and flavonoid contents that possess significant antioxidant and anticholinestrase effects. This provide pharmacological basis for the presence of compounds that may be effective in Alzheimer's and other neurological disorders.
Descritores: Saponinas/metabolismo
Flavonoides/metabolismo
Extratos Vegetais/farmacologia
Inibidores da Colinesterase/farmacologia
Atriplex/química
Doença de Alzheimer/tratamento farmacológico
Antioxidantes/farmacologia
-Fenóis/análise
Fenóis/metabolismo
Acetilcolinesterase/metabolismo
Saponinas/isolamento & purificação
Espectrofotometria
Ácidos Sulfônicos/metabolismo
Flavonoides/análise
Butirilcolinesterase/metabolismo
Inibidores da Colinesterase/isolamento & purificação
Sequestradores de Radicais Livres/farmacologia
Estresse Oxidativo/efeitos dos fármacos
Concentração Inibidora 50
Benzotiazóis/metabolismo
Medicina Tradicional
Antioxidantes/isolamento & purificação
Responsável: CL1.1 - Biblioteca Central


  4 / 10 LILACS  
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Id: lil-790193
Autor: Mancini, Evelyn Andrea; Pini Prato, Giovan Paolo.
Título: Procedimiento de descontaminación del biofilm para el tratamiento del absceso periodontal agudo y la periimplantitis / The biofilm decontamination approach for the treatment of periodontal abscess and peri-implantitis
Fonte: Rev. Asoc. Odontol. Argent;104(2):79-85, jun. 2016. ilus.
Idioma: es.
Resumo: Objetivo: mostrar el efecto de un nuevo procedimiento de descontaminación del biofilm sobre los abscesos periodontales agudos y la periimplantitis. Casos clínicos: un absceso periodontal agudo y una periimplantitis fueron tratados mediante un material de descontaminación de los tejidos bucales. Este consiste en un concentrado acuoso con una mezcla de ácidos hidroxibencensulfónicos e hidroxymetho-xybencénicos y ácido sulfúrico, que se coloca en las bolsas periodontales y alrededor de los implantes. En ninguno de los casos se utilizaron antibióticos locales ni sistémicos. Todos los casos tratados cicatrizaron rápidamente, sin complicaciones. Los pacientes sintieron una leve molestia durante la aplicación del material, que desapareció completamente en pocos segundos. Conclusión: el procedimiento de desecación del biofilm parecería ser una técnica promisoria para el tratamiento de los abscesos periodontales agudos ylas periimplantitis, con la ventaja adicional de que se evita el uso de antibióticos locales y sistémicos.
Descritores: Abscesso Periodontal/tratamento farmacológico
Biofilmes
Descontaminação/métodos
Peri-Implantite/tratamento farmacológico
-Administração Tópica
Ácidos Sulfônicos/uso terapêutico
Ácidos Sulfúricos/uso terapêutico
Bolsa Periodontal/tratamento farmacológico
Hidroxibenzoatos/uso terapêutico
Limites: Humanos
Feminino
Pessoa de Meia-Idade
Tipo de Publ: Relatos de Casos
Responsável: AR29.1 - Biblioteca


  5 / 10 LILACS  
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Id: lil-734621
Autor: Karimi, Ehsan; Jaafar, Hawa ZE; Ghasemzadeh, Ali; Ebrahimi, Mahdi.
Título: Fatty acid composition, antioxidant and antibacterial properties of the microwave aqueous extract of three varieties of Labisia pumila Benth
Fonte: Biol. Res;48:1-6, 2015. graf, tab.
Idioma: en.
Resumo: BACKGROUND: The present study was conducted in order to evaluate the fatty acid profile, anti-oxidant and anti-bacterial activities from the microwave aqueous extract of the leaves of three different varieties of Labisia pumila Benth. RESULTS: The chemical analysis of the extract showed that fatty acids (palmitic, palmitoleic, stearic, oleic, linoleic and α-linolenic) acid as the main components in three varieties of L. pumila leaves. Furthermore, the obtained results of the anti-oxidant revealed that L. pumila var. alata contained higher anti-oxidative activities compared to var. pumila and var. lanceolata. However, these values were lower than the tested anti-oxidant standards. On the other hand, the aqueous leaf extracts in all three varieties of L. pumila were also found to inhibit a variable degree of antibacterial activities against eight bacteria (four Gram-positive and four Gram-negative bacteria). CONCLUSIONS: In this study, it was observed the leaves of three varieties of L. pumila exhibited variable patterns of fatty acids and the microwave aqueous extraction possess anti-oxidant and anti-bacterial activities.
Descritores: Antibacterianos/farmacologia
Antioxidantes/farmacologia
Ácidos Graxos/análise
Micro-Ondas
Primulaceae/química
Extratos Vegetais/química
Extratos Vegetais/farmacologia
-Benzotiazóis/metabolismo
Testes de Sensibilidade a Antimicrobianos por Disco-Difusão
Flavonoides/análise
Sequestradores de Radicais Livres/análise
Bactérias Gram-Negativas/efeitos dos fármacos
Bactérias Gram-Positivas/efeitos dos fármacos
INHIBITORY CONCENTRATION ACADEMIES AND INSTITUTES
Primulaceae/classificação
Óxido Nítrico/metabolismo
Fenóis/análise
Folhas de Planta/química
Ácidos Sulfônicos/metabolismo
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


  6 / 10 LILACS  
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Texto completo SciELO Brasil
Cardoso, Arnaldo Alves
Texto completo
Id: lil-582399
Autor: Guinesi, Adriana Simionatto; Andolfatto, Carolina; Bonetti Filho, Idomeo; Cardoso, Arnaldo Alves; Passaretti Filho, Juliano; Farac, Roberta Vieira.
Título: Ozonized oils: a qualitative and quantitative analysis
Fonte: Braz. dent. j;22(1):37-40, 2011.
Idioma: en.
Resumo: Most of the problems of endodontic origin have a bacterial etiological agent. Thus, there is a continued interest in seeking more effective chemical substances that can replace the camphorated paramonochiorophenol or antibiotics as intracanal medicaments. Among the possible substances, ozone has some interesting biological characteristics: bactericidal action, debriding effect, angiogenesis stimulation capacity and high oxidizing power. The purpose of this study was to chemically evaluate the presence of ozone in sunflower, castor, olive and almond oil, as well as in propylene glycol and byproducts of ozonation, such as formaldehyde. These compounds were ozonized, inserted into empty and sterile vials, and analyzed by testing the reaction between ozone and indigo, for determining the presence of ozone, and subjected to the chromotropic acid test for determining the presence of formaldehyde. It was observed complete absence of ozone in all samples tested and presence of formaldehyde. The bactericidal and healing action of ozonized oils could be attributed to products formed by the ozonation of mineral oils, such as formaldehyde, not to the ozone itself.

A maioria dos problemas de origem endodôntica tem um agente etiológico bacteriano. Assim, existe um interesse permanente em se buscar substâncias químicas mais efetivas e que possam substituir o PMCC ou os antibióticos como curativos de demora. Dentre as possíveis substâncias, o ozônio apresenta algumas características biológicas interessantes: ação bactericida, efeito debridante, estímulo a angiogênese, além do efeito oxidante. O propósito do presente estudo foi avaliar quimicamente a presença de ozônio nos óleos de girassol, rícino, oliva e amêndoas, além do propilenoglicol, bem como subproputos da ozonização, como formaldeído. Essas substâncias foram ozonizadas, inseridas em tubetes anestésicos vazios e esterilizados, e analisadas por meio do teste da reação entre ozônio e índigo, para determinação da presença de ozônio; e teste do ácido cromotrópico, para determinação da presença de formaldeído. Foi observado ausência total de ozônio em todas as amostras testadas, além da presença de formaldeído. A ação bactericida e curativa dos óleos ozonizados poderia ser atribuída aos produtos formados pela ozonização de óleos minerais, como o formaldeído, e não ao ozônio propriamente dito.
Descritores: Ozônio/análise
Óleos Vegetais/química
Irrigantes do Canal Radicular/química
-Colorimetria
Corantes/química
Formaldeído/análise
Indóis/química
Propilenoglicol/química
Ácidos Sulfônicos/química
Responsável: BR1.1 - BIREME


  7 / 10 LILACS  
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Texto completo SciELO Brasil
Texto completo
Id: lil-485857
Autor: Asghar, M. N; Khan, I. U.
Título: Measurement of antioxidant activity with trifluoperazine dihydrochloride radical cation
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;41(6):455-461, June 2008. graf.
Idioma: en.
Projeto: Higher Education Commission of Pakistan under its Indigenous.
Resumo: A novel, rapid and cost-effective trifluoperazine dihydrochloride (TFPH) decolorization assay is described for the screening of antioxidant activity. A chromogenic reaction between TFPH and potassium persulfate at low pH produces an orange-red radical cation with maximum absorption at 502 nm in its first-order derivative spectrum. TFPH was dissolved in distilled water to give a 100 mM solution. The TFPH radical cation solution was made by reacting 0.5 mL of the solution with K2S2O8 (final concentration: 0.1 mM) and diluting to 100 mL with 4 M H2SO4 solution. A linear inhibition of color production was observed with linearly increasing amounts of antioxidants, with correlation coefficients (R²) ranging from 0.999 to 0.983. The antioxidant capacity of standard solutions of an antioxidant was evaluated by comparing with the inhibition curve using Trolox as the standard. Comparison of antioxidant capacity determined with this newly developed TFPH assay and with the well-known 2,2'-azinobis-[3-ethylbenzthiazoline-6-sulfonic acid] (ABTS)-persulfate decolorization assay indicated the efficacy and sensitivity of the procedure. The proposed assay is less expensive (costs about US$4 per 100 assays) and requires only 20 min for preparation of radical cation solution in comparison with ABTS assay, in which almost 12-16 h are required for preparation of a stable ABTS radical cation solution. The present assay has the advantage over ABTS assay that it can be used to measure the antioxidant activity of the samples, which are naturally found at a pH as low as 1, because the radical cation itself has been stabilized at low pH.
Descritores: Antioxidantes/análise
Benzotiazóis/química
Ácidos Sulfônicos/química
Trifluoperazina/química
-Cátions
Indicadores e Reagentes
Reprodutibilidade dos Testes
Espectrofotometria/métodos
Fatores de Tempo
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  8 / 10 LILACS  
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Texto completo SciELO Chile
Texto completo
Id: lil-432425
Autor: Chamy, Verónica M; Lepe, Jaime; Catalán, Álvaro; Retamal, David; Escobar, Jorge A; Madrid, Eva M.
Título: Oxidative stress is closely related to clinical severity of pre-eclampsia
Fonte: Biol. Res;39(2):229-236, 2006. ilus.
Idioma: en.
Resumo: Pre-eclampsia is a hypertensive disorder of pregnancy in which enzymatic antioxidant defenses fail and tissues are injured. This prospective case-control study evaluated whether pre-eclamptic women and their newborns show higher degrees of oxidative stress than normal pregnancies and sought to determine if this stress is related to clinical severity. Forty-four pre-eclamptic and thirty healthy pregnant women attending two hospitals in Valparaíso, Chile, were studied. The following plasmatic variables of antioxidant capacity were evaluated: glutathione peroxidase activity (GPx), total antioxidant capacity measured by oxidation of ABTS substrate (2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid), and superoxide dismutase activity (SOD). malondialdehide (MDA) was measured to evaluate lipoperoxidation. The evaluation was performed at diagnosis of pre-eclampsia, delivery, 30 days and 120 days post delivery. Newborns were studied at delivery through umbilical cord blood samples. Our analysis shows that antioxidant enzyme activity (SOD, GPx, ABTS) was significantly decreased, while lipoperoxidation (MDA) was increased in both pre-eclamptic groups compared to normal pregnant women (p<0.01). Statistically significant difference was found between mild and severe pre-eclamptic groups (p<0.01), for all biochemical markers studied. Therefore, the clinical severity of this pathology is closely related to the degree of oxidative stress.
Descritores: Peroxidação de Lipídeos
Estresse Oxidativo
Pré-Eclâmpsia/metabolismo
-Benzotiazóis/sangue
Benzotiazóis/metabolismo
Biomarcadores/sangue
Estudos de Casos e Controles
Sangue Fetal/enzimologia
Glutationa Peroxidase/sangue
Glutationa Peroxidase/metabolismo
Estudos Prospectivos
Pré-Eclâmpsia/diagnóstico
Índice de Gravidade de Doença
Ácidos Sulfônicos/sangue
Ácidos Sulfônicos/metabolismo
Superóxido Dismutase/sangue
Superóxido Dismutase/metabolismo
Substâncias Reativas com Ácido Tiobarbitúrico
Limites: Feminino
Humanos
Recém-Nascido
Gravidez
Tipo de Publ: Estudo Multicêntrico
Responsável: BR1.1 - BIREME


  9 / 10 LILACS  
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Id: lil-161667
Autor: Romay, C; Pascual, C; Lissi, E. A.
Título: The reaction between ABTS radical cation and antioxidants and its use to evaluate the antioxidant status of serum samples
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;29(2):175-83, Feb. 1996. graf, tab.
Idioma: en.
Resumo: The 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical cation can be generated by incubation of ABTS and 2,2'-azo-bis(2-amidinopropane) at 45 degrees Celsius. The ABTS radical cation is stable for several minutes at room temperature and reacts quantitatively and instantaneously with several antioxidants, such as Trolox, ascorbic acid, uric acid, cysteine, glutathione and bilirubin. In contrast, the ABTS radical cation reacts slowly with albumin. When serum is added to a solution of the ABTS radical cation, the bleaching of the radical follows biphasic kinetics, with a fast decay followed by a slow decay that takes place within several minutes. The fast decay is primarily due to uric acid, while the slow decay is related to the protein content of the sample. We propose that this procedure can provide an independent and simultaneous evaluation of the low molecular weight and protein antioxidants present in biological samples such as serum.
Descritores: Ácidos Sulfônicos/metabolismo
Antioxidantes/farmacologia
Indicadores e Reagentes/metabolismo
-Ácido Ascórbico/sangue
Ácido Úrico/sangue
Bilirrubina/sangue
Cromanos/farmacologia
Cisteína/sangue
Glutationa/sangue
Temperatura
Fatores de Tempo
Limites: Humanos
Masculino
Feminino
Responsável: BR1.1 - BIREME


  10 / 10 LILACS  
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Id: lil-148303
Autor: Davis, M.R; Zamora Silva, José; Castro, D; Henríquez, S; Hund, J; Pérez, M.
Título: Evaluación de las metódicas de la O-cresolftaleína para calcio total y calmagite para magnesio en suero sanguíneo / Evaluation of the O-cresolftalein method for total calcium and calmagite for magnesium in blood serum
Fonte: Rev. chil. cienc. méd. biol;2(1):19-23, 1992. tab, ilus.
Idioma: es.
Resumo: El calcio y el magnesio son dos importantes cationes corporales, con funciones especializadas en los líquidos orgánicos. Existen numerosos métodos para la cuantificación de estos cationes. Se evaluaron los métodos colorimétricos de la O-cresolftaleína para calcio total y del calmagite para magnesio en suero sanguíneo de 64 individuos, comparándolos con espectrofotometría de absorción atómica. Las determinaciones colorimétricas mostraron buena estabilidad de color y adecuados valores de precisión diaria e interdiaria. Al analizar los espectros de absorción, se propone modoficar las longitudes de onda establecidas en ambos métodos colorimétricos. Los valores obtenidos para el calcio con la O-cresolftaleína, resultaron similares a los obtenidos con espectrofotometría de absorción atómica; en cambio para el magnesio las determinaciones con Calmagite fueron en promedio 8,7 por ciento más altos
Descritores: Cálcio/sangue
Colorimetria
Espectrofotometria Atômica/métodos
Magnésio/sangue
-Ácidos Sulfônicos
Kit de Reagentes para Diagnóstico/normas
Limites: Humanos
Adulto
Tipo de Publ: Estudo Comparativo
Responsável: CL1.1 - Biblioteca Central



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BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde