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Id: biblio-1052260
Autor: Huang, Lin; Zheng, Dong; Zhao, Yatong; Ma, Jieying; Li, Yanzhen; Xu, Zehua; Shan, Mengying; Shao, Shulin; Guo, Qingwen; Zhang, Jie; Lu, Fuping; Liu, Yihan.
Título: Improvement of the alkali stability of Penicillium cyclopium lipase by error-prone PCR
Fonte: Electron. j. biotechnol;39:91-97, may. 2019. ilus, graf, tab.
Idioma: en.
Projeto: National Key R&D Program of China; . National Natural Science Foundation of China; . China Postdoctoral Science Foundation; . Natural Science Foundation of Tianjin City.
Resumo: BACKGROUND: Lipases are extensively exploited in lots of industrial fields; cold-adapted lipases with alkali-resistance are especially desired in detergent industry. Penicillium cyclopium lipase I (PCL) might be suitable for applications of detergent industry due to its high catalytic efficiency at low temperature and relatively good alkali stability. In this study, to better meet the requirements, the alkali stability of PCL was further improved via directed evolution with error-prone PCR. RESULTS: The mutant PCL (N157F) with an improved alkali stability was selected based on a high-throughput activity assay. After incubating at pH 11.0 for 120 min, N157F retained 70% of its initial activity, which was 23% higher than that of wild type PCL. Combined with the three-dimensional structure analysis, N157F exhibited an improved alkali stability under the high pH condition due to the interactions of hydrophilicity and ß-strand propensity. Conclusions: This work provided the theoretical foundation and preliminary data for improving alkali stability of PCL to meet the industrial requirements, which is also beneficial to improving alkali-tolerance ability of other industrial enzymes via molecular modification.
Descritores: Penicillium/enzimologia
Estabilidade Enzimática
Indústria de Detergentes
Lipase/metabolismo
-Penicillium/isolamento & purificação
Penicillium/genética
Reação em Cadeia da Polimerase/métodos
Temperatura Baixa
Álcalis
Biocatálise
Interações Hidrofóbicas e Hidrofílicas
Concentração de Íons de Hidrogênio
Lipase/isolamento & purificação
Lipase/genética
Mutação
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1135444
Autor: Tostes, Joanna Gonçalves de Andrade; Dias, Rafaela Toledo; Reis, Andressa Aparecida da Silva; Silveira, Pollyanna Santos da; Ronzani, Telmo Mota.
Título: Interventions to Reduce Stigma Related to People who Use Drugs: Systematic Review / Intervenções para Redução do Estigma Relacionado às Pessoas que Usam Drogas: Revisão Sistemática / Intervenciones para la Reducción del Estigma Relacionado a las Personas que Usan Drogas: Revisión Sistemática
Fonte: Paidéia (Ribeirão Preto, Online);30:e3022, 2020. tab, graf.
Idioma: en.
Projeto: FAPEMIG; . by CNPq.
Resumo: Abstract Interventions to reduce stigma related to people who use drugs can improve their quality of life and adherence to treatment. This review aimed to identify and analyze studies on interventions to reduce the stigma related to people who use drugs. Searches were performed in the following databases, without period delimitation: PubmMed, APA PsycNET, ScienceDirect, Web of Science, and VHL. Considering secondary referencing, we identified a total of 5,488 records. This review covered 28 articles and was based on PRISMA. Although target audiences comprising students and health professionals were predominant, as well as the use of quantitative methods, sample size and interventions varied among studies. The selected studies present considerable methodological limitations. We concluded that no evidence confirms the effectiveness of the proposed interventions and which of them should continue to be applied for this specific aim. It is essential to invest in approaches other than those traditionally adopted.

Resumo Intervenções para a redução do estigma relacionado às pessoas que usam drogas podem melhorar sua qualidade de vida e adesão ao tratamento. Esta revisão teve por objetivo identificar e analisar estudos de intervenções para reduzir o estigma relacionado às pessoas que usam drogas. Foram realizadas buscas nas bases PubMed, APA PsycNET, ScienceDirect, Web of Science e BVS, sem delimitação de ano. Foram identificados 5.488 registros somados à busca secundária de referências. Esta revisão abrange 28 artigos e foi baseada no PRISMA. Houve variação no tamanho da amostra e intervenções, embora o público-alvo de estudantes e profissionais da saúde e o uso de métodos quantitativos tenham sido predominantes. Limitações metodológicas consideráveis foram identificadas nos estudos. Portanto, conclui-se que não há evidências que permitam inferir que as intervenções propostas são efetivas e quais delas deveriam continuar a ser empregadas para essa finalidade. É imprescindível investir em abordagens distintas das adotadas tradicionalmente.

Resumen Las intervenciones para la reducción del estigma relacionado a las personas que usan drogas pueden mejorar su calidad de vida y la adherencia al tratamiento. Esta revisión tuvo como objetivo identificar y analizar los estudios sobre las intervenciones para la reducción del estigma relacionado a las personas que usan drogas. Se realizaron búsquedas en las bases de datos PubMed, APA PsycNET, ScienceDirect, Web of Science y BVS, sin delimitación del año. Se identificaron 5.488 registros sumados a la búsqueda secundaria de referencias. Esta revisión abarca 28 artículos y se basó en PRISMA. Hubo una variación en el tamaño de la muestra y las intervenciones, aunque fueron predominantes el público objetivo de estudiantes y profesionales de la salud y el uso de métodos cuantitativos. Se identificaron limitaciones metodológicas considerables en los estudios. Se concluye que no hay evidencias de que las intervenciones propuestas son efectivas y cuáles deberán seguir siendo utilizadas para este propósito. Es esencial invertir en enfoques distintos de los adoptados tradicionalmente.
Descritores: Psicologia
Qualidade de Vida
Cristianismo
Pessoal de Saúde
Transtornos Relacionados ao Uso de Substâncias
Álcalis
Estigma Social
Tipo de Publ: Revisão
Responsável: BR26.1 - Biblioteca Central


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Id: biblio-1128504
Autor: Ferreira, K. D; Cardoso, L. D; Oliveira, L. P; Franzo, V. S; Pancotti, A; Miguel, M. P; Silva, L. A. F; Vulcani, V. A. S.
Título: Histological analysis of elastic cartilages treated with alkaline solution / Análise histológica de cartilagens elásticas tratadas com solução alcalina
Fonte: Arq. bras. med. vet. zootec. (Online);72(3):647-654, May-June, 2020. ilus, tab, graf.
Idioma: en.
Projeto: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil.
Resumo: The elastic cartilage is composed by chondroblasts and chondrocytes, extracellular matrix and surrounded by perichondrium. It has a low regeneration capacity and is a challenge in surgical repair. One of obstacles in engineering a structurally sound and long-lasting tissue is selecting the most appropriate scaffold material. One of the techniques for obtaining biomaterials from animal tissues is the decellularization that decreases antigenicity. In this work, alkaline solution was used in bovine ear elastic cartilages to evaluate the decellularization and the architecture of the extracellular matrix. The cartilages were treated in alkaline solution (pH13) for 72 hours and lyophilized to be compared with untreated cartilages by histological analysis (hematoxylin-eosin, Masson's trichrome and Verhoeff slides). Areas of interest for cell counting and elastic fiber quantification were delineated, and the distribution of collagen and elastic fibers and the presence of non-fibrous proteins were observed. The results demonstrated that the alkaline solution caused 90% decellularization in the middle and 13% in the peripheral region, and maintenance of the histological characteristics of the collagen and elastic fibers and non-fibrous protein removal. It was concluded that the alkaline solution was efficient in the decellularization and removal of non-fibrous proteins from the elastic cartilages of the bovine ear.(AU)

A cartilagem elástica é composta por condroblastos e condrócitos, matriz extracelular e envolta por pericôndrio. Possui uma baixa capacidade de regeneração e é um desafio em reparos cirúrgicos. Um dos obstáculos na engenharia de tecido estruturalmente sólido e de longa duração é a seleção do material de arcabouço mais adequado. Uma das técnicas para obtenção de biomateriais oriundos de tecidos animais é a descelularização, que diminui a antigenicidade. Neste trabalho, foi utilizada solução alcalina em cartilagem elástica auricular bovina para avaliar a descelularização e a arquitetura da matriz extracelular. As cartilagens foram tratadas em solução alcalina (pH13) durante 72 horas e liofilizadas, e comparadas com cartilagens não tratadas por análise histológica (hematoxilina-eosina, tricrômio de Masson e Verhoeff). Foram determinadas as áreas de interesse para contagem celular e quantificação de fibras elásticas, observada a distribuição de colágeno e fibras elásticas e a presença de proteínas não fibrosas. Os resultados demonstraram que a solução alcalina causou 90% de descelularização na região central e 13% na região periférica, manutenção das características histológicas do colágeno e fibras elásticas e remoção das proteínas não fibrosas. Concluiu-se que a solução alcalina foi eficiente na descelularização e retirada de proteínas não fibrosas de cartilagens elásticas da orelha de bovinos.(AU)
Descritores: Materiais Biocompatíveis
Condrócitos
Engenharia Tecidual/veterinária
Cartilagem Elástica
Matriz Extracelular
-Bovinos
Cartilagem
Amarelo de Eosina-(YS)
Álcalis
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: biblio-1126969
Autor: Lopes-Júnior, Luís Carlos; Rosa, Gabriela Sylvestre; Pessanha, Raphael Manhães; Schuab, Sara Isabel Pimentel de Carvalho; Nunes, Karolini Zuqui; Amorim, Maria Helena Costa.
Título: Efficacy of the complementary therapies in the management of cancer pain in palliative care: A systematic review / Eficácia das terapias complementares no manejo da dor oncológica em cuidados paliativos: revisão sistemática / Eficacia de las terapias complementarias en el manejo del dolor oncológico en los cuidados paliativos: revisión sistemática
Fonte: Rev. latinoam. enferm. (Online);28:e3377, 2020. tab, graf.
Idioma: en.
Projeto: FAPES.
Resumo: Objective: to synthesize the knowledge and to critically evaluate the evidences arising from randomized controlled trials on the efficacy of the complementary therapies in the management of cancer pain in adult patients with cancer in palliative care. Method: a systematic review guided by the Preferred Reporting Items for Systematic Reviews and Meta-Analyses. The search for articles in the MEDLINE, ISI Web of Knowledge, CENTRAL Cochrane, and PsycINFO databases, as well as the manual search, selection of studies, data extraction, and methodological assessment using the Cochrane Bias Risk tool were performed independently by two reviewers. Results: eight hundred and fifteen (815) studies were identified, six of them being selected and analyzed, of which three used massage therapy, one study used a combination of progressive muscle relaxation and guided imaging, and another two studies used acupuncture. Most of the studies had an uncertain risk of bias (n=4; 67%). Conclusion: while the evidence from the studies evaluating the use of massage therapy or the use of progressive muscle relaxation and guided imaging for the management of cancer pain in these patients demonstrated significant benefits, the other two studies that evaluated the use of acupuncture as a complementary therapy showed contradictory results, therefore, needing more research studies to elucidate such findings.

Objetivo: sintetizar o conhecimento e avaliar criticamente as evidências provenientes de ensaios clínicos controlados randomizados sobre a eficácia das terapias complementares no manejo da dor oncológica em pacientes adultos com câncer em cuidados paliativos. Método: revisão sistemática guiada pelo Preferred Reporting Items for Systematic Reviews and Meta-Analyses. A busca dos artigos nas bases de dados MEDLINE, ISI Web of Knowledge, CENTRAL Cochrane e PsycINFO, bem como a busca manual, seleção dos estudos, extração dos dados e avaliação metodológica pela ferramenta do Risco de Viés da Cochrane foram realizadas por dois revisores de forma independente. Resultados: foram identificados 815 estudos, sendo seis selecionados e analisados, dos quais três utilizaram a massagem terapêutica, um estudo usou uma combinação de relaxamento muscular progressivo e imagem guiada, e outros dois estudos a acupuntura. A maioria dos estudos apresentou risco de viés incerto (n=4; 67%). Conclusão: enquanto as evidências dos estudos que avaliaram o uso da massagem terapêutica ou o uso de relaxamento muscular progressivo e imagem guiada para manejo da dor oncológica nesses pacientes mostraram benefícios significativos, os outros dois estudos que avaliaram o uso da acupuntura como terapia complementar exibiram resultados divergentes, necessitando, portanto, de mais pesquisas para elucidar tais achados.

Objetivo: sintetizar conocimiento y realizar el análisis crítico de las evidencias procedentes de ensayos clínicos controlados aleatorios sobre la eficacia de las terapias complementarias en el manejo del dolor oncológico en pacientes adultos con cáncer en cuidados paliativos. Método: revisión sistemática guiada por el Preferred Reporting Items for Systematic Reviews and Meta-Analyses. La búsqueda de artículos en las bases de datos MEDLINE, ISI Web of Knowledge, CENTRAL Cochrane y PsycINFO, así como la búsqueda manual, selección de estudios, extracción de datos y evaluación metodológica con la utilización de la herramienta Riesgo de Sesgo de Cochrane se realizaron por dos revisores de forma independiente. Resultados: se identificaron 815 estudios, de los cuales, seis fueron seleccionados y analizados, siendo que tres utilizaron masaje terapéutico, un estudio utilizó una combinación de relajación muscular progresiva e imagen guiada, y otros dos estudios la acupuntura. La mayor parte de los estudios presentaron riesgo de sesgo incierto (n=4; 67%) Conclusión: mientras las evidencias de los estudios que evalúan el uso de la del masaje terapéutico o el uso de la relajación muscular progresiva y las imágenes guiadas para el manejo del dolor oncológico en estos pacientes demostraron beneficios significativos, los otros dos estudios que evaluaron el uso de la acupuntura como terapia complementaria mostraron resultados divergentes, razón por la cual se requiere de más investigación para dilucidar tales hallazgos.
Descritores: Enfermagem Oncológica
Cuidados Paliativos
Terapêutica
Terapias Complementares
Eficácia
Imagens, Psicoterapia
Indicadores de Qualidade em Assistência à Saúde
Acupuntura
Álcalis
Enfermagem Baseada em Evidências
Dor do Câncer
Massagem
Relaxamento Muscular
Neoplasias
Limites: Humanos
Masculino
Feminino
Adulto
Tipo de Publ: Revisão
Responsável: BR26.1 - Biblioteca Central


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Id: biblio-974331
Autor: Guo, Ruiting; Wang, Zhiying; Huang, Ying; Fan, Haijuan; Liu, Zhihua.
Título: Biocontrol potential of saline- or alkaline-tolerant Trichoderma asperellum mutants against three pathogenic fungi under saline or alkaline stress conditions
Fonte: Braz. j. microbiol;49(supl.1):236-245, 2018. graf.
Idioma: en.
Projeto: National High Technology Research and Development Program (the 13th Five-Year Plan Program); . Fundamental Research Funds for the Central Universities.
Resumo: ABSTRACT Salinity and alkalinity are major abiotic stresses that limit growth and development of poplar. We investigated biocontrol potential of saline- and alkaline-tolerant mutants of Trichoderma asperellum to mediate the effects of salinity or alkalinity stresses on Populus davidiana × P. alba var. pyramidalis (PdPap poplar) seedlings. A T-DNA insertion mutant library of T. asperellum was constructed using an Agrobacterium tumefaciens mediated transformation system; this process yielded sixty five positive transformants (T1-T65). The salinity tolerant mutant, T59, grew in Potato Dextrose Agar (PDA) containing up to 10% (1709.40 mM) NaCl. Under NaCl-rich conditions, T59 was most effective in inhibiting Alternaria alternata (52.00%). The alkalinity tolerant mutants, T3 and T5, grew in PDA containing up to 0.4% (47.62 mM) NaHCO3. The ability of the T3 and T5 mutants to inhibit Fusarium oxysporum declined as NaHCO3 concentrations increased. NaHCO3 tolerance of the PdPap seedlings improved following treatment with the spores of the WT, T3, and T5 strains. The salinity tolerant mutant (T59) and two alkalinity tolerant mutants (T3 and T5) generated in this study can be applied to decrease the incidence of pathogenic fungi infection under saline or alkaline stress.
Descritores: Doenças das Plantas/microbiologia
Trichoderma/fisiologia
Cloreto de Sódio/metabolismo
Populus/crescimento & desenvolvimento
Álcalis/metabolismo
Alternaria/fisiologia
Antibiose
-Doenças das Plantas/prevenção & controle
Estresse Fisiológico
Trichoderma/genética
Populus/microbiologia
Plântula/crescimento & desenvolvimento
Plântula/microbiologia
Responsável: BR1.1 - BIREME


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Id: biblio-973685
Autor: Barrón Balderas, Alejandro; Robledo Aceves, Mireya; Coello Ramírez, Pedro; García Rodríguez, Elizabeth; Barriga Marín, Javier A.
Título: Hallazgos endoscópicos en el tubo digestivo secundarios a la ingesta de cáusticos en niños atendidos en el Departamento de Urgencias / Endoscopic findings of the digestive tract secondary to caustic ingestion in children seen at the Emergency Department
Fonte: Arch. argent. pediatr;116(6):409-414, dic. 2018. ilus, tab.
Idioma: en; es.
Resumo: Antecedentes. La ingesta de sustancias cáusticas en pediatría constituye una causa frecuente de atención médica en Urgencias. El uso indiscriminado de limpiadores químicos y la facilidad de adquisición son factores determinantes para estas lesiones. Población y métodos. Estudio descriptivo analítico. Se incluyeron niños < 16 años, entre enero de 1998 y diciembre de 2017. Se identificó el agente químico cáustico ingerido por el niño como ácido o alcalino. Se realizó una endoscopía digestiva para tipificar el grado de quemadura. Se comparó el grado de quemadura con el tipo de cáustico; por medio de la prueba de chi² o exacta de Fisher, se consideró significativo el valor de P < 0,05. Resultados. Ingresaron 133 niños en Urgencias por ingesta de cáusticos. El agente cáustico fue ácido en el 41 % y alcalino en el 59 %. El agente ácido más frecuente fue el ácido muriático (36,8 %), mientras que el alcalino fue la sosa (soda) cáustica (41,4 %). La quemadura del esófago fue más frecuente en la ingesta de sosa en comparación con otros cáusticos (p= 0,001), mientras que la quemadura del estómago (p= 0,001) y del duodeno (p= 0,002) fue estadísticamente significativa en la ingesta de ácido muriático. El grupo de edad que más frecuentemente ingirió algún cáustico (93,2 %) fueron los menores de 5 años. Conclusiones. El cáustico ingerido con mayor frecuencia fue un agente alcalino, que provocó quemadura esofágica, mientras que un agente ácido provocó quemaduras en el estómago y el duodeno evidenciadas por endoscopía.

Background. Caustic ingestion in pediatrics is a common cause of visits to the Emergency Department. An indiscriminate use of cleaning chemicals and an easy access to them are determining factors for these injuries. Population and methods. Descriptive, analytical study. Children aged < 16 years hospitalized between January 1998 and December 2017 were included. The ingested caustic substance was identified as acid or alkaline. A gastrointestinal endoscopy was done to establish the burn grade. The grade of the burn was compared to the type of caustic substance using the χ² test or the Fisher's exact test; a P value < 0.05 was considered significant. Results. A total of 133 children were admitted to the Emergency Department due to caustic ingestion. The caustic agent was acid in 41 % of cases and alkaline, in 59 %. The most common acid caustic substance was muriatic acid (36.8 %) and the most common alkaline caustic agent was caustic soda (41.4 %). An esophageal burn was the most common consequence of caustic soda ingestion compared to other caustic agents (p = 0.001), whereas muriatic acid ingestion was the most statistically significant cause of stomach burn (p = 0.001) and duodenal burn (p = 0.002). The age group that most commonly ingested some caustic agent (93.2 %) corresponded to children younger than 5 years. Conclusions. The most common type of ingested caustic agent was alkaline, which caused esophageal burn; whereas, the ingestion of an acid caustic substance caused stomach and duodenal burns, as evidenced by endoscopy.
Descritores: Queimaduras Químicas/diagnóstico
Cáusticos/envenenamento
Endoscopia Gastrointestinal/métodos
Trato Gastrointestinal/lesões
-Estômago/lesões
Ácidos/envenenamento
Queimaduras Químicas/etiologia
Queimaduras Químicas/epidemiologia
Epidemiologia Descritiva
Fatores Etários
Duodeno/lesões
Álcalis/envenenamento
Serviço Hospitalar de Emergência
Esôfago/lesões
Limites: Humanos
Lactente
Pré-Escolar
Criança
Adolescente
Responsável: AR94.1 - Centro de Información Pediatrica


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Id: lil-735403
Autor: Mejías Sánchez, Yoerquis; Morales Suárez, Ileana; Perera Vandrell, Sebastián.
Título: Bases legales y proyecciones de desarrollo de la metrología en el Sistema Nacional de Salud / Legal basis and development projection of metrology in the national health care system
Fonte: Rev. cuba. salud pública;41(1):172-177, ene.-mar. 2015.
Idioma: es.
Resumo: La metrología es una ciencia de amplia aplicación en los servicios de salud puesto que garantiza la objetividad, confiabilidad, trazabilidad y uniformidad de las mediciones. Es propósito de este informe presentar las bases legales de la metrología en Cuba y sus perspectivas de desarrollo en el Sistema Nacional de Salud. Se realizó una búsqueda acerca de la pertinencia de esta ciencia y de las proyecciones del Ministerio de Salud Pública para su desarrollo. En Cuba, la metrología está refrendada en los Lineamientos de la Política Económica y Social del Partido y la Revolución y soportada legalmente. En el sector de la salud, el aseguramiento metrológico lo apoya, fundamentalmente, el Servicio Nacional de Metrología y la red de servicios de electromedicina, que dentro de sus funciones tiene la de reparar y calibrar equipos e instrumentos de medición. La existencia de las nuevas tecnologías y los requerimientos del Sistema conllevan a la implementación, por parte del Ministerio de Salud Pública, de un conjunto de acciones dirigidas al desarrollo de la metrología, entre ellas, la formación y superación de los recursos humanos, el desarrollo de las inversiones, la utilización de los resultados de la ciencia, el control y las alianzas estratégicas. Todos son elementos claves para garantizar las actividades de aseguramiento metrológico que demanda el Sistema Nacional de Salud y contribuir, desde esa óptica, al progreso de la salud pública, al mejoramiento de la calidad de los servicios y a la satisfacción de la población.

Metrology is science of great application in health care services since it assures objectivity, reliability, traceability and uniformity of measurements. The objective of the present report was to present the legal bases of metrology in Cuba and its development prospects in the Cuban health care system. A search was made on the relevance of this science and the projections of the Ministry of Public Health for its advancement. Metrology is included in the Guidelines of the Economic and Social Policy of the Party and the Revolution and is legally endorsed in Cuba. The National Service of Metrology and the medical equipment maintenance and repair service are the ones that render the metrological assurance in the health care sector. One of their functions is to repair and calibrate measurement tools and equipment. The new technologies and the requirements of the national health care system lead to the implementation of a set of actions by the Ministry of Public Health towards the development of metrology such as formation and upgrading of human resources, investment plans, utilization of the scientific results, control and strategic alliances. All of these are key elements to guarantee the metrological assurance activities that the health system demands, thus contributing to the advance of the public health, the improvement of the service quality and the satisfaction of the population.
Descritores: Medidas, Métodos e Teorias
Álcalis
Limites: Humanos
Responsável: CU1.1 - Biblioteca Médica Nacional


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Id: lil-775128
Autor: Alrumman, Sulaiman A..
Título: Enzymatic saccharification and fermentation of cellulosic date palm wastes to glucose and lactic acid
Fonte: Braz. j. microbiol;47(1):110-119, Jan.-Mar. 2016. tab, graf.
Idioma: en.
Resumo: Abstract The bioconversion of cellulosic wastes into high-value bio-products by saccharification and fermentation processes is an important step that can reduce the environmental pollution caused by agricultural wastes. In this study, enzymatic saccharification of treated and untreated date palm cellulosic wastes by the cellulases from Geobacillus stearothermophilus was optimized. The alkaline pre-treatment of the date palm wastes was found to be effective in increasing the saccharification percentage. The maximum rate of saccharification was found at a substrate concentration of 4% and enzyme concentration of 30 FPU/g of substrate. The optimum pH and temperature for the bioconversions were 5.0 and 50 °C, respectively, after 24 h of incubation, with a yield of 31.56 mg/mL of glucose at a saccharification degree of 71.03%. The saccharification was increased to 94.88% by removal of the hydrolysate after 24 h by using a two-step hydrolysis. Significant lactic acid production (27.8 mg/mL) was obtained by separate saccharification and fermentation after 72 h of incubation. The results indicate that production of fermentable sugar and lactic acid is feasible and may reduce environmental pollution by using date palm wastes as a cheap substrate.
Descritores: Celulases/metabolismo
Celulose/metabolismo
Geobacillus stearothermophilus/enzimologia
Glucose/metabolismo
Resíduos Industriais
Ácido Láctico/metabolismo
Phoeniceae/metabolismo
-Álcalis
Biotransformação
Fermentação
Concentração de Íons de Hidrogênio
Phoeniceae/efeitos dos fármacos
Temperatura
Responsável: BR1.1 - BIREME


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Id: lil-775115
Autor: Luvielmo, Márcia de Mello; Borges, Caroline Dellinghausen; Toyama, Daniela de Oliveira; Vendruscolo, Claire Tondo; Scamparini, Adilma Regina Pippa.
Título: Structure of xanthan gum and cell ultrastructure at different times of alkali stress
Fonte: Braz. j. microbiol;47(1):102-109, Jan.-Mar. 2016. graf.
Idioma: en.
Resumo: Abstract The effect of alkali stress on the yield, viscosity, gum structure, and cell ultrastructure of xanthan gum was evaluated at the end of fermentation process of xanthan production by Xanthomonas campestris pv. manihotis 280-95. Although greater xanthan production was observed after a 24 h-alkali stress process, a lower viscosity was observed when compared to the alkali stress-free gum, regardless of the alkali stress time. However, this outcome is not conclusive as further studies on gum purification are required to remove excess sodium, verify the efficiency loss and the consequent increase in the polymer viscosity. Alkali stress altered the structure of xanthan gum from a polygon-like shape to a star-like form. At the end of the fermentation, early structural changes in the bacterium were observed. After alkali stress, marked structural differences were observed in the cells. A more vacuolated cytoplasm and discontinuities in the membrane cells evidenced the cell lysis. Xanthan was observed in the form of concentric circles instead of agglomerates as observed prior to the alkali stress.
Descritores: Álcalis/toxicidade
Polissacarídeos Bacterianos/química
Polissacarídeos Bacterianos/metabolismo
Estresse Fisiológico
Xanthomonas campestris/metabolismo
Xanthomonas campestris/ultraestrutura
-Membrana Celular/ultraestrutura
Citoplasma/ultraestrutura
Organelas/ultraestrutura
Xanthomonas campestris/efeitos dos fármacos
Responsável: BR1.1 - BIREME


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Id: lil-741276
Autor: Colavolpe, María Belén; Mejía, Santiago Jaramillo; Albertó, Edgardo.
Título: Efficiency of treatments for controlling Trichoderma spp during spawning in cultivation of lignicolous mushrooms
Fonte: Braz. j. microbiol;45(4):1263-1270, Oct.-Dec. 2014. ilus, tab.
Idioma: en.
Resumo: Trichoderma spp is the cause of the green mold disease in mushroom cultivation production. Many disinfection treatments are commonly applied to lignocellulose substrates to prevent contamination. Mushroom growers are usually worried about the contaminations that may occur after these treatments during handling or spawning. The aim of this paper is to estimate the growth of the green mold Trichoderma sp on lignocellulose substrates after different disinfection treatments to know which of them is more effective to avoid contamination during spawning phase. Three different treatments were assayed: sterilization (121 ºC), immersion in hot water (60 and 80 ºC), and immersion in alkalinized water. Wheat straw, wheat seeds and Eucalyptus or Populus sawdust were used separately as substrates. After the disinfection treatments, bagged substrates were sprayed with 3 mL of suspension of conidia of Trichoderma sp (10(5) conidia/mL) and then separately spawned with Pleurotus ostreatus or Gymnopilus pampeanus. The growth of Trichoderma sp was evaluated based on a qualitative scale. Trichoderma sp could not grow on non-sterilized substrates. Immersions in hot water treatments and immersion in alkalinized water were also unfavorable treatments for its growth. Co- cultivation with mushrooms favored Trichoderma sp growth. Mushroom cultivation disinfection treatments of lignocellulose substrates influence on the growth of Trichoderma sp when contaminations occur during spawning phase. The immersion in hot water at 60 ºC for 30 min or in alkalinized water for 36 h, are treatments which better reduced the contaminations with Trichoderma sp during spawning phase for the cultivation of lignicolous species.
Descritores: Agaricales/crescimento & desenvolvimento
Desinfecção/métodos
Trichoderma/crescimento & desenvolvimento
-Álcalis/metabolismo
Anti-Infecciosos/metabolismo
Eucalyptus/microbiologia
Temperatura Alta
Populus/microbiologia
Temperatura
Trichoderma/efeitos dos fármacos
Trichoderma/efeitos da radiação
Triticum/microbiologia
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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