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Pesquisa : D01.142 [Categoria DeCS]
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Texto completo SciELO Brasil
Texto completo
Id: lil-730425
Autor: Shojaei, Taha Roodbar; Salleh, Mohamad Amran Mohd; Tabatabaei, Meisam; Ekrami, Alireza; Motallebi, Roya; Rahmani-Cherati, Tavoos; Hajalilou, Abdollah; Jorfi, Raheleh.
Título: Development of sandwich-form biosensor to detect Mycobacterium tuberculosis complex in clinical sputum specimens
Fonte: Braz. j. infect. dis;18(6):600-608, Nov-Dec/2014. tab, graf.
Idioma: en.
Resumo: Mycobacterium tuberculosis, the causing agent of tuberculosis, comes second only after HIV on the list of infectious agents slaughtering many worldwide. Due to the limitations behind the conventional detection methods, it is therefore critical to develop new sensitive sensing systems capable of quick detection of the infectious agent. In the present study, the surface modified cadmium-telluride quantum dots and gold nanoparticles conjunct with two specific oligonucleotides against early secretory antigenic target 6 were used to develop a sandwich-form fluorescence resonance energy transfer-based biosensor to detect M. tuberculosis complex and differentiate M. tuberculosis and M. bovis Bacille Calmette–Guerin simultaneously. The sensitivity and specificity of the newly developed biosensor were 94.2% and 86.6%, respectively, while the sensitivity and specificity of polymerase chain reaction and nested polymerase chain reaction were considerably lower, 74.2%, 73.3% and 82.8%, 80%, respectively. The detection limits of the sandwich-form fluorescence resonance energy transfer-based biosensor were far lower (10 fg) than those of the polymerase chain reaction and nested polymerase chain reaction (100 fg). Although the cost of the developed nanobiosensor was slightly higher than those of the polymerase chain reaction-based techniques, its unique advantages in terms of turnaround time, higher sensitivity and specificity, as well as a 10-fold lower detection limit would clearly recommend this test as a more appropriate and cost-effective tool for large scale operations.
Descritores: Técnicas Biossensoriais/métodos
Mycobacterium bovis/isolamento & purificação
Mycobacterium tuberculosis/isolamento & purificação
Tuberculose Pulmonar/diagnóstico
-Compostos de Cádmio
Transferência Ressonante de Energia de Fluorescência/instrumentação
Transferência Ressonante de Energia de Fluorescência/métodos
Nanopartículas Metálicas
Reação em Cadeia da Polimerase
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
Limites: Seres Humanos
Responsável: BR1.1 - BIREME

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Texto completo SciELO Brasil
Cesar, Carlos Lenz
Texto completo
Id: lil-583939
Autor: Vieira, Cecilia Stahl; Almeida, Diogo Burigo; Thomaz, André Alexandre de; Menna-Barreto, Rubem Figueredo Sadok; Santos-Mallet, Jacenir Reis dos; Cesar, Carlos Lenz; Gomes, Suzete Araujo Oliveira; Feder, Denise.
Título: Studying nanotoxic effects of CdTe quantum dots in Trypanosoma cruzi
Fonte: Mem. Inst. Oswaldo Cruz;106(2):158-165, Mar. 2011. ilus, graf, tab.
Idioma: en.
Resumo: Semiconductor nanoparticles, such as quantum dots (QDs), were used to carry out experiments in vivo and ex vivo with Trypanosoma cruzi. However, questions have been raised regarding the nanotoxicity of QDs in living cells, microorganisms, tissues and whole animals. The objective of this paper was to conduct a QD nanotoxicity study on living T. cruzi protozoa using analytical methods. This was accomplished using in vitro experiments to test the interference of the QDs on parasite development, morphology and viability. Our results show that after 72 h, a 200 μM cadmium telluride (CdTe) QD solution induced important morphological alterations in T. cruzi, such as DNA damage, plasma membrane blebbing and mitochondrial swelling. Flow cytometry assays showed no damage to the plasma membrane when incubated with 200 μM CdTe QDs for up to 72 h (propidium iodide cells), giving no evidence of classical necrosis. Parasites incubated with 2 μM CdTe QDs still proliferated after seven days. In summary, a low concentration of CdTe QDs (2 μM) is optimal for bioimaging, whereas a high concentration (200 μM CdTe) could be toxic to cells. Taken together, our data indicate that 2 μM QD can be used for the successful long-term study of the parasite-vector interaction in real time.
Descritores: Compostos de Cádmio/toxicidade
Proliferação Celular
Dano ao DNA
Pontos Quânticos
Trypanosoma cruzi
-Membrana Celular
Citometria de Fluxo
Corantes Fluorescentes
Microscopia Eletrônica de Transmissão
Dilatação Mitocondrial
Trypanosoma cruzi/ultraestrutura
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME

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