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Id: biblio-837935
Autor: Hoefel, Isadora da Rosa; Camozzato, Fernanda Oliveira; Hagemann, Laura Netto; Rhoden, Deise Louise Bohn; Kiszewski, Ana Elisa.
Título: Bromoderma in an infant
Fonte: An. bras. dermatol;91(5,supl.1):17-19, Sept.-Oct. 2016. graf.
Idioma: en.
Resumo: Abstract Bromoderma is a cutaneous eruption caused by the absorption of bromide. Clinical manifestations include acneiform and vegetative lesions. We report the case of an infant with bromoderma caused by the use of syrup for abdominal colic containing calcium bromide. The lesions regressed after discontinuation of the drug.
Descritores: Brometos/efeitos adversos
Erupção por Droga/etiologia
Erupção por Droga/patologia
Compostos de Cálcio/efeitos adversos
-Pele/patologia
Cólica/tratamento farmacológico
Erupções Acneiformes/induzido quimicamente
Erupções Acneiformes/patologia
Limites: Seres Humanos
Masculino
Lactente
Tipo de Publ: Relatos de Casos
Responsável: BR1.1 - BIREME


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Id: lil-785019
Autor: Silva, Sérgio Ribeiro da; Silva Neto, José Dias da; Schnaider, Taylor Brandão; Veiga, Daniela Francescato; Novo, Neil Ferreira; Mesquita Filho, Marcos; Ferreira, Lydia Masako.
Título: The use of a biocompatible cement in endodontic surgery. A randomized clinical trial 1
Fonte: Acta cir. bras;31(6):422-427graf.
Idioma: en.
Resumo: ABSTRACT PURPOSE: To evaluate the clinical applicability of Pozzolana Biologic Silva cement (PBS(r)) in endodontic surgery. METHODS: Persistent apical periodontitis was diagnosed in 30 teeth of 12 patients by cone-beam computed tomography (CT). All patients had 2 or 4 affected teeth and underwent endodontic surgery with root-end filling. Patients with 2 affected teeth had one tooth (control) treated with mineral trioxide aggregate (MTA-Angelus(r)) as a root-end filling material, and the other tooth treated with PBS (experiment). When the patient had four affected teeth, two of them were treated with MTA and two with PBS. Six months after surgery, all patients were assessed by CT scan. Between-group comparisons of measurements were performed using the Wilcoxon test. RESULTS: Periradicular tissue regeneration was observed in all cases. Significant within-group differences in long axes of the lesion were found in the bucco-palatal direction (PBS group, p=0.0012; MTA group, p=0.024) and coronal-apical direction (PBS group, p=0.0007; MTA group, p=0.0015) between pre- and postoperative measurements. CONCLUSIONS: Pozzolana Biologic Silva cement can be used in the treatment of persistent periradicular lesions. The clinical use of PBS as a root-end filling material may be an alternative to MTA. PBS has additives, which provide enhanced strength.
Descritores: Óxidos/uso terapêutico
Periodontite Periapical/cirurgia
Materiais Biocompatíveis/uso terapêutico
Compostos de Cálcio/uso terapêutico
Compostos de Alumínio/uso terapêutico
Cimentos Dentários/uso terapêutico
-Apicectomia/métodos
Periodontite Periapical/diagnóstico por imagem
Período Pós-Operatório
Regeneração/fisiologia
Raiz Dentária/cirurgia
Raiz Dentária/diagnóstico por imagem
Estudos Prospectivos
Combinação de Medicamentos
Tomografia Computadorizada de Feixe Cônico
Limites: Seres Humanos
Adolescente
Adulto
Meia-Idade
Adulto Jovem
Tipo de Publ: Ensaio Clínico Controlado Aleatório
Responsável: BR1.1 - BIREME


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Id: lil-787546
Autor: GUERREIRO-TANOMARU, Juliane Maria; VÁZQUEZ-GARCÍA, Fernando Antonio; BOSSO-MARTELO, Roberta; BERNARDI, Maria Inês Basso; FARIA, Gisele; TANOMARU FILHO, Mario.
Título: Effect of addition of nano-hydroxyapatite on physico-chemical and antibiofilm properties of calcium silicate cements
Fonte: J. appl. oral sci;24(3):204-210tab, graf.
Idioma: en.
Projeto: São Paulo Research Foundation; . São Paulo Research Foundation.
Resumo: ABSTRACT Objective Mineral Trioxide Aggregate (MTA) is a calcium silicate cement composed of Portland cement (PC) and bismuth oxide. Hydroxyapatite has been incorporated to enhance mechanical and biological properties of dental materials. This study evaluated physicochemical and mechanical properties and antibiofilm activity of MTA and PC associated with zirconium oxide (ZrO2) and hydroxyapatite nanoparticles (HAn). Material and Methods White MTA (Angelus, Brazil); PC (70%)+ZrO2 (30%); PC (60%)+ZrO2 (30%)+HAn (10%); PC (50%)+ZrO2 (30%)+HAn (20%) were evaluated. The pH was assessed by a digital pH-meter and solubility by mass loss. Setting time was evaluated by using Gilmore needles. Compressive strength was analyzed by mechanical test. Samples were radiographed alongside an aluminum step wedge to evaluate radiopacity. For the antibiofilm evaluation, materials were placed in direct contact with E. faecalis biofilm induced on dentine blocks. The number of colony-forming units (CFU mL-1) in the remaining biolfilm was evaluated. The results were submitted to ANOVA and the Tukey test, with 5% significance. Results There was no difference in pH levels of PC+ZrO2, PC+ZrO2+HAn (10%) and PC+ZrO2+HAn (20%) (p>0.05) and these cements presented higher pH levels than MTA (p<0.05). The highest solubility was observed in PC+ZrO2+HAn (10%) and PC+ZrO2+HAn (20%) (p<0.05). MTA had the shortest initial setting time (p<0.05). All the materials showed radiopacity higher than 3 mmAl. PC+ZrO2 and MTA had the highest compressive strength (p<0.05). Materials did not completely neutralize the bacterial biofilm, but the association with HAn provided greater bacterial reduction than MTA and PC+ZrO2 (p<0.05) after the post-manipulation period of 2 days. Conclusions The addition of HAn to PC associated with ZrO2 harmed the compressive strength and solubility. On the other hand, HAn did not change the pH and the initial setting time, but improved the radiopacity (HAn 10%), the final setting time and the E. faecalis antibiofilm activity of the cement.
Descritores: Óxidos/química
Zircônio/química
Enterococcus faecalis/efeitos dos fármacos
Silicatos/química
Durapatita/química
Compostos de Cálcio/química
Compostos de Alumínio/química
Biofilmes/efeitos dos fármacos
Nanopartículas/química
-Óxidos/farmacologia
Solubilidade
Fatores de Tempo
Zircônio/farmacologia
Bismuto/farmacologia
Bismuto/química
Teste de Materiais
Contagem de Colônia Microbiana
Análise de Variância
Enterococcus faecalis/crescimento & desenvolvimento
Silicatos/farmacologia
Durapatita/farmacologia
Compostos de Cálcio/farmacologia
Compostos de Alumínio/farmacologia
Força Compressiva
Cimentos Dentários/farmacologia
Cimentos Dentários/química
Combinação de Medicamentos
Concentração de Íons de Hidrogênio
Tipo de Publ: Estudos de Avaliação
Responsável: BR1.1 - BIREME


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Id: biblio-893669
Autor: Garcia, Lucas da Fonseca Roberti; Huck, Claudia; Magalhães, Fernando Augusto Cintra; Souza, Pedro Paulo Chaves de; Souza Costa, Carlos Alberto de.
Título: Systemic effect of mineral aggregate-based cements: histopathological analysis in rats
Fonte: J. appl. oral sci;25(6):620-630, Nov.-Dec. 2017. tab, graf.
Idioma: en.
Projeto: CNPq.
Resumo: Abstract Objective: Several studies reported the local tissue reaction caused by mineral aggregate-based cements. However, few studies have investigated the systemic effects promoted by these cements on liver and kidney when directly applied to connective tissue. The purpose of this in vivo study was to investigate the systemic effect of mineral aggregate-based cements on the livers and kidneys of rats. Material and Methods: Samples of Mineral Trioxide Aggregate (MTA) and a calcium aluminate-based cement (EndoBinder) containing different radiopacifiers were implanted into the dorsum of 40 rats. After 7 and 30 d, samples of subcutaneous, liver and kidney tissues were submitted to histopathological analysis. A score (0-3) was used to grade the inflammatory reaction. Blood samples were collected to evaluate changes in hepatic and renal functions of animals. Results: The moderate inflammatory reaction (2) observed for 7 d in the subcutaneous tissue decreased with time for all cements. The thickness of inflammatory capsules also presented a significant decrease with time (P<.05). Systemically, all cements caused adverse inflammatory reactions in the liver and kidney, being more evident for MTA, persisting until the end of the analysis. Liver functions increased significantly for MTA during 30 d (P<.05). Conclusion: The different cements induced to a locally limited inflammatory reaction. However, from the systemic point of view, the cements promoted significant inflammatory reactions in the liver and kidney. For MTA, the reactions were more accentuated.
Descritores: Óxidos/farmacologia
Materiais Restauradores do Canal Radicular/farmacologia
Silicatos/farmacologia
Compostos de Cálcio/farmacologia
Compostos de Alumínio/farmacologia
Cimentos Dentários/farmacologia
Rim/efeitos dos fármacos
Fígado/efeitos dos fármacos
-Fatores de Tempo
Materiais Biocompatíveis
Teste de Materiais
Combinação de Medicamentos
Rim/patologia
Fígado/patologia
Limites: Animais
Masculino
Ratos
Responsável: BR1.1 - BIREME


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Id: biblio-893667
Autor: Lee, Bin-Na; Chun, Soo-Ji; Chang, Hoon-Sang; Hwang, Yun-Chan; Hwang, In-Nam; Oh, Won-Mann.
Título: Physical properties and biological effects of mineral trioxide aggregate mixed with methylcellulose and calcium chloride
Fonte: J. appl. oral sci;25(6):680-688, Nov.-Dec. 2017. tab, graf.
Idioma: en.
Projeto: Chonnam National University Hospital Biomedical Research Institute; . Korea government (MSIP).
Resumo: Abstract Objectives: Methylcellulose (MC) is a chemical compound derived from cellulose. MTA mixed with MC reduces setting time and increases plasticity. This study assessed the influence of MC as an anti-washout ingredient and CaCl2 as a setting time accelerator on the physical and biological properties of MTA. Material and Methods: Test materials were divided into 3 groups; Group 1(control): distilled water; Group 2: 1% MC/CaCl2; Group 3: 2% MC/CaCl2. Compressive strength, pH, flowability and cell viability were tested. The gene expression of bone sialoprotein (BSP) was detected by RT-PCR and real­ time PCR. The expression of alkaline phosphatase (ALP) and mineralization behavior were evaluated using an ALP staining and an alizarin red staining. Results: Compressive strength, pH, and cell viability of MTA mixed with MC/CaCl2 were not significantly different compared to the control group. The flowability of MTA with MC/CaCI2 has decreased significantly when compared to the control (p<.05). The mRNA level of BSP has increased significantly in MTA with MC/CaCl2 compared to the control (p<.05). This study revealed higher expression of ALP and mineralization in cells exposed to MTA mixed with water and MTA mixed with MC/CaCl2 compared to the control (p<.05). Conclusions: MC decreased the flowability of MTA and did not interrupt the physical and biological effect of MTA. It suggests that these cements may be useful as a root-end filling material.
Descritores: Óxidos/farmacologia
Óxidos/química
Materiais Restauradores do Canal Radicular/química
Cloreto de Cálcio/farmacologia
Silicatos/farmacologia
Silicatos/química
Compostos de Cálcio/farmacologia
Compostos de Cálcio/química
Compostos de Alumínio/farmacologia
Compostos de Alumínio/química
Metilcelulose/farmacologia
-Teste de Materiais
Células Cultivadas/efeitos dos fármacos
Força Compressiva
Polpa Dentária/efeitos dos fármacos
Combinação de Medicamentos
Limites: Animais
Camundongos
Responsável: BR1.1 - BIREME


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Cirelli, Joni Augusto
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Id: biblio-893632
Autor: TORRES, Fernanda Ferrari Esteves; BOSSO-MARTELO, Roberta; ESPIR, Camila Galletti; CIRELLI, Joni Augusto; GUERREIRO-TANOMARU, Juliane Maria; TANOMARU-FILHO, Mario.
Título: Evaluation of physicochemical properties of root-end filling materials using conventional and Micro-CT tests
Fonte: J. appl. oral sci;25(4):374-380, July-Aug. 2017. tab, graf.
Idioma: en.
Projeto: São Paulo Research Foundation; . São Paulo Research Foundation; . São Paulo Research Foundation.
Resumo: Abstract Objective To evaluate solubility, dimensional stability, filling ability and volumetric change of root-end filling materials using conventional tests and new Micro-CT-based methods. Material and Methods Solubility (loss of mass) after 7 and 30 days, and dimensional stability (in mm) were evaluated in accordance with Carvalho-Junior, et al. 7 (2007). The filling ability and volumetric change (in mm3) were evaluated by Micro-CT (Bruker-MicroCT, Kontich, Belgium) using resin models with cavities 3 mm deep and 1 mm in diameter. The cavities were filled with materials to evaluate filling ability, and then scanned by Micro-CT. After 7 and 30 days immersed in distilled water, the filled cavities were scanned again to evaluate the volumetric change. MTA Angelus (MTA), Biodentine (BIO) and zinc oxide-eugenol cement (ZOE) were evaluated. Data were submitted to analysis of variance (ANOVA) and Tukey's test with 5% significance level. Results The results suggested correlated or complementary data between the proposed tests. At 7 days, BIO showed higher solubility and at 30 days, showed higher volumetric change in comparison with MTA (p<0.05). With regard to volumetric change, the tested materials were similar (p>0.05) at 7 days. At 30 days, they presented similar solubility. BIO and MTA showed higher dimensional stability than ZOE (p<0.05). ZOE and BIO showed higher filling ability (p<0.05). Conclusions ZOE presented a higher dimensional change, and BIO had greater solubility after 7 days. BIO presented filling ability and dimensional stability, but greater volumetric change than MTA after 30 days. Micro-CT can provide important data on the physicochemical properties of materials complementing conventional tests.
Descritores: Óxidos/química
Materiais Restauradores do Canal Radicular/química
Cimento de Óxido de Zinco e Eugenol/química
Bismuto/química
Silicatos/química
Compostos de Cálcio/química
Microtomografia por Raio-X/métodos
-Valores de Referência
Solubilidade
Fatores de Tempo
Teste de Materiais
Reprodutibilidade dos Testes
Análise de Variância
Modelos Dentários
Tipo de Publ: Estudos de Avaliação
Responsável: BR1.1 - BIREME


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Costa, Carlos Alberto de Souza
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Id: biblio-888644
Autor: Moraes, Paola Castro de; Marques, Isabela Cristina de Souza; Basso, Fernanda Gonçalves; Rossetto, Hebert Luis; Pires-de-Souza, Fernanda de Carvalho Panzeri; Costa, Carlos Alberto de Souza; Garcia, Lucas da Fonseca Roberti.
Título: Repair of Bone Defects with Chitosan-Collagen Biomembrane and Scaffold Containing Calcium Aluminate Cement
Fonte: Braz. dent. j;28(3):287-295, May-June 2017. tab, graf.
Idioma: en.
Projeto: \"National Council for Scientific and Technological Development - CNPq\".
Resumo: Abstract Innovative biomaterials can provide a promising new direction for the treatment of bone defects, stimulating a proper repair process, with no damage to adjacent tissues. The purpose of this in vivo study was to evaluate the biocompatibility and the osteoinductive capacity of chitosan-collagen biomembrane and scaffold containing calcium aluminate cement. Eighteen New Zealand white rabbits (Oryctolagus cuniculus) were distributed according to the experimental times of analysis (7, 15 and 30 days). Four bone defects were created in the rabbits calvaria, which were individually filled with the biomembrane, scaffold, blood clot (negative control) and autologous bone (positive control). Histopathological analysis was performed using optical microscope at 32´, 64´, 125´ and 320´ magnifications. Cell response to inflammation and new bone tissue formation was quantified using a score system. The biomembrane group presented greater inflammatory response at 15 days, with significant difference to autologous bone group (p<0.05). There was no statistically significant difference for foreign body type reaction among groups (p>0.05). Concerning new bone formation, linear closure of the defect area was observed more evidently in the group with autologous bone. The scaffold group presented similar results compared with the autologous bone group at 30 days (p>0.05). Both tested biomaterials presented similar biocompatibility compared with the control groups. In addition, the biomembrane and scaffold presented similar osteoinductive capacity, stimulating bone repair process in the course of the experimental time intervals.

Resumo Biomateriais inovadores podem fornecer uma promissora nova direção para o tratamento de defeitos ósseos, estimulando um processo de reparo adequado, sem danos aos tecidos adjacentes. O objetivo deste estudo in vivo foi avaliar a biocompatibilidade e a capacidade osteoindutora de uma biomembrana e um scaffold compostos por colágeno e quitosana, contendo cimento de aluminato de cálcio. Dezoito coelhos (New Zealand White, Oryctolagus cuniculus) foram distribuídos de acordo com os períodos experimentais de análise (7, 15 e 30 dias). Quatro defeitos foram criados na calvaria dos coelhos, que foram individualmente preenchidos com a biomembrana, scaffold, coágulo (controle negativo) e osso autólogo (controle positivo). A avaliação histopatológica foi realizada em microscópio óptico em aumentos de 32´, 64´, 125´ e 320´. A resposta celular à inflamação e à formação de novo tecido ósseo foi quantificada utilizando um sistema de escore. O grupo da biomembrana apresentou maior resposta inflamatória no período de 15 dias, com diferença significativa para o grupo do osso autólogo (p<0,05). Não houve diferença estatística significante para a reação do tipo corpo estranho entre os grupos (p>0,05). Em relação à neoformação óssea, observou-se fechamento linear da área do defeito, que foi mais evidente no grupo em que se utilizou o osso autólogo. O grupo scaffold apresentou resultados semelhantes ao grupo do osso autólogo no período de 30 dias (p>0,05). Ambos os biomateriais testados apresentaram biocompatibilidade similar em comparação com os grupos controle. Além disso, a biomembrana e o scaffold apresentaram capacidade osteoindutora similar, estimulando o reparo ósseo ao longo dos intervalos de tempo experimentais.
Descritores: Materiais Biocompatíveis
Osso e Ossos/efeitos dos fármacos
Colágeno/química
Compostos de Cálcio/química
Compostos de Alumínio/química
Cimentos Dentários/química
Quitosana/química
Tecidos Suporte
Membranas Artificiais
-Osso e Ossos/anormalidades
Desenvolvimento Ósseo
Reação a Corpo Estranho/patologia
Inflamação/patologia
Limites: Animais
Coelhos
Responsável: BR1.1 - BIREME


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Id: biblio-841179
Autor: CORRAL NUÑEZ, Camila; COVARRUBIAS, Cristian; FERNANDEZ, Eduardo; OLIVEIRA JUNIOR, Osmir Batista de.
Título: Enhanced bioactive properties of BiodentineTM modified with bioactive glass nanoparticles
Fonte: J. appl. oral sci;25(2):177-185, Mar.-Apr. 2017. graf.
Idioma: en.
Projeto: Fondecyt.
Resumo: Abstract Objective To prepare nanocomposite cements based on the incorporation of bioactive glass nanoparticles (nBGs) into BiodentineTM (BD, Septodent, Saint-Maur-des-Fosses Cedex, France) and to assess their bioactive properties. Material and Methods nBGs were synthesised by the sol-gel method. BD nanocomposites (nBG/BD) were prepared with 1 and 2% nBGs by weight; unmodified BD and GC Fuji IX (GIC, GC Corporation, Tokyo, Japan) were used as references. The in vitro ability of the materials to induce apatite formation was assessed in SBF by X-ray diffraction (XRD), attenuated total reflectance with Fourier transform infrared spectroscopy (ATR-FTIR), and scanning electron microscopy (SEM) with energy dispersive X-ray (EDX) analysis. BD and nBG/BD were also applied to dentine discs for seven days; the morphology and elemental composition of the dentine-cement interface were analysed using SEM-EDX. Results One and two percent nBG/BD composites accelerated apatite formation on the disc surface after short-term immersion in SBF. Apatite was detected on the nBG/BD nanocomposites after three days, compared with seven days for unmodified BD. No apatite formation was detected on the GIC surface. nBG/BD formed a wider interfacial area with dentine than BD, showing blockage of dentine tubules and Si incorporation, suggesting intratubular precipitation. Conclusions The incorporation of nBGs into BD improves its in vitro bioactivity, accelerating the formation of a crystalline apatite layer on its surface after immersion in SBF. Compared with unmodified BD, nBG/BD showed a wider interfacial area with greater Si incorporation and intratubular precipitation of deposits when immersed in SBF.
Descritores: Silicatos/química
Compostos de Cálcio/química
Dentina/efeitos dos fármacos
Nanopartículas/química
Cimentos de Ionômeros de Vidro/química
-Apatitas/química
Espectrometria por Raios X
Propriedades de Superfície/efeitos dos fármacos
Fatores de Tempo
Difração de Raios X
Teste de Materiais
Microscopia Eletrônica de Varredura
Reprodutibilidade dos Testes
Espectroscopia de Infravermelho com Transformada de Fourier
Estatísticas não Paramétricas
Cimentos de Resina/química
Imersão
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


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Costa, Carlos Alberto de Souza
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Id: biblio-839113
Autor: Huck, Claudia; Barud, Hernane da Silva; Basso, Fernanda Gonçalves; Costa, Carlos Alberto de Souza; Hebling, Josimeri; Garcia, Lucas da Fonseca Roberti.
Título: Cytotoxicity of New Calcium Aluminate Cement (EndoBinder) Containing Different Radiopacifiers
Fonte: Braz. dent. j;28(1):57-64, Jan.-Feb. 2017. tab, graf.
Idioma: en.
Projeto: “Foundation for University Estadual Paulista Development” (FUNDUNESP; . Unesp; . “National Council for Scientific and Technological Development” (CNPq.
Resumo: Abstract This study aimed to evaluate the cytotoxicity of a calcium aluminate cement (EndoBinder) containing different radiopacifiers, Bi2O3, ZnO or ZrO2, compared with Mineral Trioxide Aggregate (MTA). According to ISO 10993-12:2012 (E) recommendations, 0.2 g of each cement were applied in transwell inserts and placed in 24-well culture plates containing 1 mL of culture medium (DMEM). After 24 h of incubation, the extracts (DMEM containing components released from the cements) were applied to immortalized odontoblast-like MDPC-23 cells. Cell viability (MTT test), alkaline phosphatase activity (ALP), total protein production and cell morphology (Scanning Electron Microscopy - SEM) were evaluated. The volume of 50 µL of extract was used to determine the chemical elements released by the cements using Energy Dispersive Spectroscopy (EDS). The following groups were established (n=6): NC - negative control (without treatment); EB - EndoBinder without radiopacifier; EBBO - EndoBinder+Bi2O3; EBZnO - EndoBinder+ZnO; EBZrO - EndoBinder+ZrO2 and WMTA - White MTA. Data were subjected to statistical analysis (Kruskal-Wallis test, level of significance=5%). Cells exposed to the different versions of EndoBinder presented small reduction in viability, total protein production and ALP activity, with values similar to the NC and WMTA groups (p>0.05). Different elements (C, O, Na, Al, P, Si, Cl, Bi, K) released by the cements were detected in the extracts. However, the cells had no significant changes in their morphology. EndoBinder and MTA did not affect negatively the metabolism of the odontoblastic-like cells, showing it to be cytocompatible, irrespective of the used radiopacifier.

Resumo Este estudo avaliou a citotoxicidade de um cimento de aluminato de cálcio (EndoBinder) contendo diferentes radiopacificadores, Bi2O3, ZnO ou ZrO2, comparativamente ao trióxido mineral agregado (MTA). Seguindo a norma ISO 10993-12:2012 (E), 0,2 g de cada cimento foi aplicada em insertos transwell, que foram colocados em placas de cultura de 24 wells contendo 1 mL de meio de cultura (DMEM). Após 24 h de incubação, os extratos (DMEM contendo componentes liberados dos cimentos) foram aplicados sobre células pulpares imortalizadas MDPC-23. Viabilidade celular (teste de MTT), atividade da fosfatase alcalina (ALP), produção de proteína total e a morfologia das células (Microscópio Eletrônico de Varredura - MEV) foram avaliadas. Um volume de 50 µL do extrato foi utilizado para determinar, através de Espectroscopia de Energia Dispersiva (EDS), os elementos químicos liberados pelos cimentos. Os seguintes grupos foram estabelecidos (n=6): NC - controle negativo (sem tratamento); EB - EndoBinder sem radiopacificador; EBBO - EndoBinder+Bi2O3; EBZnO - EndoBinder+ZnO; EBZrO - EndoBinder+ZrO2 e WMTA - MTA branco. Os dados foram submetidos à análise estatística (teste de Kruskal-Wallis, nível de significância=5%). Células expostas às diferentes versões de EndoBinder apresentaram pequena redução na viabilidade, produção de proteína total e atividade da ALP, com valores semelhantes aos grupos NC e WMTA (p>0,05). Diversos elementos (C, O, Na, Al, P, Si, Cl, Bi, K) liberados pelos cimentos foram detectados nos extratos. Entretanto, as células não apresentaram alterações significativas em sua morfologia. EndoBinder e MTA, não afetaram negativamente o metabolismo das células odontoblastóides, mostrando-se citocompatíveis, independente do radiopacificador utilizado.
Descritores: Compostos de Cálcio/toxicidade
Compostos de Alumínio/toxicidade
Cimentos Dentários/toxicidade
-Óxidos/toxicidade
Espectrometria por Raios X
Linhagem Celular Transformada
Microscopia Eletrônica de Varredura
Sobrevivência Celular/efeitos dos fármacos
Silicatos/toxicidade
Combinação de Medicamentos
Limites: Animais
Responsável: BR1.1 - BIREME


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Id: biblio-839107
Autor: Mestieri, Leticia Boldrin; Gomes-Cornélio, Ana Lívia; Rodrigues, Elisandra Márcia; Faria, Gisele; Guerreiro-Tanomaru, Juliane Maria; Tanomaru-Filho, Mário.
Título: Cytotoxicity and Bioactivity of Calcium Silicate Cements Combined with Niobium Oxide in Different Cell Lines
Fonte: Braz. dent. j;28(1):65-71, Jan.-Feb. 2017. tab, graf.
Idioma: en.
Projeto: FAPESP; . CNPq.
Resumo: Abstract The aim of this study was to evaluate the cytotoxicity and bioactivity of calcium silicate-based cements combined with niobium oxide (Nb2O5) micro and nanoparticles, comparing the response in different cell lines. This evaluation used four cell lines: two primary cultures (human dental pulp cells - hDPCs and human dental follicle cells - hDFCs) and two immortalized cultures (human osteoblast-like cells - Saos-2 and mouse periodontal ligament cells - mPDL). The tested materials were: White Portland Cement (PC), mineral trioxide aggregate (MTA), white Portland cement combined with microparticles (PC/Nb2O5µ) or nanoparticles (PC/Nb2O5n) of niobium oxide (Nb2O5). Cytotoxicity was evaluated by the methylthiazolyldiphenyl-tetrazolium bromide (MTT) and trypan blue exclusion assays and bioactivity by alkaline phosphatase (ALP) enzyme activity. Results were analyzed by ANOVA and Tukey test (a=0.05). PC/Nb2O5n presented similar or higher cell viability than PC/Nb2O5µ in all cell lines. Moreover, the materials presented similar or higher cell viability than MTA. Saos-2 exhibited high ALP activity, highlighting PC/Nb2O5µ material at 7 days of exposure. In conclusion, calcium silicate cements combined with micro and nanoparticles of Nb2O5 presented cytocompatibility and bioactivity, demonstrating the potential of Nb2O5 as an alternative radiopacifier agent for these cements. The different cell lines had similar response to cytotoxicity evaluation of calcium silicate cements. However, bioactivity was more accurately detected in human osteoblast-like cell line, Saos-2.

Resumo O objetivo deste estudo foi avaliar a citotoxicidade e bioatividade de cimentos à base de silicato de cálcio associados com óxido de nióbio (Nb2O5) micro e nanoparticulados, e comparar a resposta em diferentes linhagens celulares. Foram utilizadas quatro linhagens celulares: duas culturas primárias (células da polpa dentária humana - hDPCs e células do folículo dentário humano - hDFCs) e duas culturas imortalizadas (células osteoblásticas humanas - Saos-2 e células do ligamento periodontal de ratos - mPDL). Os materiais analisados foram: Cimento Portland branco (PC); Agregado trióxido mineral (MTA); PC associado com micropartículas (PC/Nb2O5µ) ou nanopartículas (PC/Nb2O5n) de óxido de nióbio (Nb2O5). A citotoxicidade foi avaliada pelos ensaios de brometo de metil-tiazolil-difeniltetrazólio (MTT) e azul de tripan, e a bioatividade pela atividade da enzima fosfatase alcalina (ALP). Os resultados foram analisados por ANOVA e teste de Tukey (a=0,05). O grupo do PC/Nb2O5n apresentou viabilidade celular semelhante ou maior do que o grupo do PC/Nb2O5μ em todas as linhagens celulares. Além disso, ambos os grupos apresentaram viabilidade celular semelhante ou maior do que o MTA. Saos-2 apresentaram maior atividade de ALP, com destaque para o material PC/Nb2O5μ aos 7 dias de exposição. Concluiu-se que cimentos de silicato de cálcio associados com Nb2O5 micro ou nanoparticulado apresentaram citocompatibilidade e bioatividade, demonstrando potencial do Nb2O5 como agente radiopacificador alternativo para estes cimentos. As linhagens celulares estudadas apresentaram resposta semelhante na avaliação da citotoxicidade de cimentos de silicato de cálcio. No entanto, a bioatividade é melhor detectada na linhagem de células osteoblásticas humanas, Saos-2.
Descritores: Óxidos/farmacologia
Silicatos/farmacologia
Compostos de Cálcio/farmacologia
Cimentos Dentários/farmacologia
Nióbio/farmacologia
-Linhagem Celular
Fosfatase Alcalina/metabolismo
Limites: Seres Humanos
Animais
Camundongos
Responsável: BR1.1 - BIREME



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