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Id: biblio-1091461
Autor: Fundaoglu Küçükekenci, Funda; Serkan Küçükekenci, Ahmet; Kutalmis Büyük, Süleyman.
Título: Effects of an Antioxidant on the Shear Bond Strengths of Orthodontic Brackets After Conventional and Laser-Assisted Extra-Coronal Bleaching / Efecto de un antioxidante sobre la fuerza de unión de brackets de ortodoncia posterior al blanqueamiento convencional y asistido por láser
Fonte: Odovtos (En línea);20(3):71-79, Sep.-Dec. 2018. tab.
Idioma: es.
Resumo: RESUMEN El objetivo del presente estudio fue evaluar los efectos de la aplicación de un antioxidante sobre las fuerzas de unión (SBS) de brackets de ortodoncia posterior al blanqueamiento con peróxido de hidrógeno (HP). Metodología: Cincuenta premolares recién extraídos se dividieron aleatoriamente en tres grupos. El Grupo 1 se trató con 40% de HP (n=20), el grupo 2 con 40% de HP y se activó con láser de Nd: YAG (n=20) y un grupo de control (Grupo 3) no recibió tratamiento (n=10). Después del blanqueamiento, los Grupos 1 y 2 se dividieron en dos subgrupos (Grupo 1a, Grupo 1b, Grupo 2a y Grupo 2b). Las muestras en los subgrupos 1a y 2a (n=10) no recibieron ninguna aplicación del antioxidante. Se aplicó una solución de ascorbato de sodio al 10% a las muestras en los Grupos 1b y 2b (n=10). Luego, los brackets se adherieron a las superficies de esmalte de todos los grupos experimentales. Las muestras se sometieron a pruebas de SBS y los datos analizados estadisticamente. Resultados: La fuerza de unión de los grupos 1 y 2 fue significativamente más baja que el grupo de control (p <0.001); sin embargo, no se observaron diferencias estadísticamente significativas en SBS entre los grupos 1 y 2 (p>0.001). Las muestras tratadas con antioxidantes de los grupos 1b y 2b fueron significativamente mayores que las de los grupos 1a y 2a (p <0,001). Conclusion: La aplicación de 40% HP tuvo un efecto negativo en la resistencia de unión de los brackets. El uso de antioxidante durante 15 minutos mejoró significativamente la SBS.

ABSTRACT Purpose: The purpose of this study was to evaluate the effects of the application of antioxidant on the shear bond strengths (SBS) of orthodontic brackets bonded to human enamel after extra-coronal bleaching with hydrogen peroxide (HP). Materials and methods: Fifty freshly extracted premolars were randomly divided into three groups. One group (Group 1) was bleached with 40% HP (n=20), another group (Group 2) was bleached with 40% HP and activated with an Nd: YAG laser (n=20), and a control group (Group 3) received no treatment (n=10). After the bleaching procedure, Groups 1 and 2 were further divided into two subgroups (Group 1a, Group 1b, Group 2a, and Group 2b). Specimens in Groups 1a and 2a (n=10) received no antioxidant application. A 10% sodium ascorbate solution was applied to specimens in Groups 1b and 2b (n=10). The brackets were then bonded with light-cure adhesive paste to the enamel surfaces of all experimental groups. Specimens were subjected to SBS tests for debonding. Results: The SBS of brackets bonded in Groups 1 and 2 were significantly lower than those of brackets bonded to the control group (p<0.001); however, no statistically significant differences in SBS were noted in Groups 1 and 2 (p>0.001). The antioxidant-treated SBS of brackets in Groups 1b and 2b were significantly higher than those of Groups 1a and 2a (p<0.001). Conclusions: The application of 40% HP had a negative effect on the bond strength. The use of antioxidant for 15 min resulted in the reversal of failed bond strength.
Descritores: Clareamento Dental/métodos
Braquetes Ortodônticos
Resistência ao Cisalhamento/efeitos dos fármacos
Peróxido de Hidrogênio/efeitos adversos
-Terapia a Laser
Tipo de Publ: Ensaio Clínico
Responsável: CR1.1 - BINASSS - Biblioteca Nacional de Salud y Seguridad Social


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Id: biblio-975767
Autor: Vilchez-Fuentes-Rivera, Katherine; Rumiche, Francisco Aurelio; Tay, Lidia Yileng.
Título: Efecto del Extracto de Maíz Morado "Chicha Morada" durante el blanqueamiento Dental. In vitro / Effect of Purple Corn Beverage "Chicha Morada" During Dental Bleaching. In vitro
Fonte: Int. j. odontostomatol. (Print);12(4):416-422, dic. 2018. tab, graf.
Idioma: es.
Resumo: RESUMEN: El resultado del tratamiento de blanqueamiento dental puede verse afectado en pacientes que consumen bebidas pigmentantes durante el tratamiento. El objetivo de este trabajo consistió en valuar el efecto in vitro de la exposición al extracto de maíz morado (chicha morada) sobre el color del esmalte humano, durante y después del tratamiento del blanqueamiento dental con peróxido de hidrógeno al 35 %. Se utilizaron 48 dientes humanos, divididos en grupos según la bebida a la que se expuso: Extracto de maíz morado peruano (MM), té verde (T) y agua destilada (A); la mitad de los especímenes expuestos a cada bebida fueron sometidos a blanqueamiento dental con peróxido de hidrógeno al 35 % durante los primeros días de exposición a la pigmentación, resultando en los siguientes grupos: Grupo A: Sin blanqueamiento + maíz morado, Grupo B: Sin blanqueamiento + té verde, Grupo C: Sin blanqueamiento + agua destilada, Grupo D: Con blanqueamiento + maíz morado, Grupo E: Con blanqueamiento + té verde, Grupo F: Con blanqueamiento + agua destilada. Los cambios de color se midieron con un espectrofotómetro digital (VITA Easyshade Advance 4.0, VITA, Alemania) antes del blanqueamiento, durante el blanqueamiento, finalizado el blanqueamiento y al final de los 36 días de exposición a los pigmentos. Según el ∆E, el extracto de maíz morado difiere significativamente con el agua destilada (p < 0,05). Con respecto al grado de luminosidad, el extracto de maíz morado con blanqueamiento presentó los menores valores de luminosidad (p < 0,05). En cuanto al croma, no hubo diferencias entre los grupos (p > 0,05). La exposición al extracto de maíz morado pigmenta los dientes, durante el blanqueamiento el extracto de maíz morado no afecta el tratamiento, pero si la exposición continúa luego del blanqueamiento dental, el color de los dientes se verá afectado.

ABSTRACT: The result of tooth whitening treatment may be affected in patients who consume staining drinks during treatment. The aim of this work was to evaluate the in vitro effect of the exposure to purple corn extract (chicha morada) on human enamel color, during and after the treatment of teeth whitening with 35 % hydrogen peroxide. Forty-eight human teeth were used, divided into groups according to the drink to which it was exposed: Peruvian purple corn extract (MM), green tea (T) and distilled water (A); half of the specimens exposed to each drink were subjected to tooth whitening with 35 % hydrogen peroxide during the first days of exposure to pigmentation, resulting in the following groups: Group A: Without bleaching + purple corn, Group B: Without whitening + green tea, Group C: No whitening + distilled water, Group D: With whitening + purple corn, Group E: With whitening + green tea, Group F: With whitening + distilled water. The color changes were measured with a digital spectrophotometer (VITA Easyshade Advance 4.0, VITA, Germany) before bleaching, during whitening, after whitening and at the end of 36 days of exposure to pigments. According to the ∆E, the purple corn extract differs significantly with the distilled water (p <0.05). Regarding the degree of luminosity, the purple corn extract with whitening presented the lowest luminosity values (p <0.05). As for the chroma, there were no differences between the groups (p> 0.05). Exposure to purple corn extract pigments the teeth, during whitening the purple corn extract does not affect the treatment, but if the exposure continues after tooth whitening, the color of the teeth will be affected.
Descritores: Pigmentos Biológicos/química
Clareamento Dental
Clareadores Dentários
-Peru
Bebidas
Técnicas In Vitro
Espectrofotômetros
Comissão de Ética
Zea mays/classificação
Zea mays/química
Sensibilidade da Dentina
Peróxido de Hidrogênio
Limites: Humanos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1285553
Autor: Santos, Beatriz Vieira dos; Travaini, Rodolfo; Lorenzo-Hernando, Ana; Pasquini, Daniel; Baffi, Milla Alves.
Título: Biomass Sorghum: Effect of Acid, Basic and Alkaline Peroxide Pretreatments on the Enzymatic Hydrolysis and Ethanol Production
Fonte: Braz. arch. biol. technol;64:e21200117, 2021. tab.
Idioma: en.
Projeto: regional government of Castilla y León.
Resumo: Abstract This study evaluated the effects of three chemical pretreatments of biomass sorghum (BS): dilute alkaline (PTA1 and PTA2), dilute acid (PTB1 and PTB2) and alkaline hydrogen peroxide (PTC1 and PTC2) in the enzymatic hydrolysis and ethanol production. Among the six investigated conditions, the pretreatment with 7.36% H2O2 (PTC2) was the most efficient in the lignin removal and preservation of the polysaccharide fraction. After the enzymatic hydrolysis, increases in the glucose and xylose concentrations were observed in the pretreated BS hydrolysates, mainly in PTB1 and PTC1. All the hydrolysates obtained low concentrations of inhibitors. In the alcoholic fermentations with Pichia stiptis, the greatest ethanol yield was obtained in PTB1 hydrolysate (3.84 g L-1), corresponding to 16.15% of yield. The highest ethanol yield in PTB1 hydrolysate can be justified by the maximum concentration of xylose obtained in this hydrolysate, demonstrating the potential of P. stiptis in the fermentation of pentose to ethanol. The results indicated that biomass sorghum is an alternative lignocellulose source with potential for the production of second generation ethanol, opening up prospects for additional studies.
Descritores: Biomassa
Etanol
-Fenômenos Químicos
Peróxido de Hidrogênio
Metais Alcalinos
Responsável: BR1.1 - BIREME


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Id: biblio-1154098
Autor: Tovani-Palone, Marcos Roberto; Shamsoddin, Erfan.
Título: Use of mouthwashes in the management of COVID-19 patients in intensive care units: recommendations and current evidence / Uso de colutórios no manejo de pacientes com COVID-19 em unidades de terapia intensiva: recomendações e evidências atuais
Fonte: Einstein (Säo Paulo);19:eCE6419, 2021. tab, graf.
Idioma: en.
Descritores: Ventiladores Mecânicos/efeitos adversos
Pneumonia Associada a Assistência à Saúde/prevenção & controle
COVID-19/prevenção & controle
Anti-Infecciosos Locais/administração & dosagem
Antissépticos Bucais/administração & dosagem
-Povidona-Iodo/administração & dosagem
Cetilpiridínio/administração & dosagem
Cuidados Críticos
SARS-CoV-2
Peróxido de Hidrogênio/administração & dosagem
Unidades de Terapia Intensiva
Anti-Infecciosos Locais/uso terapêutico
Antissépticos Bucais/uso terapêutico
Limites: Humanos
Tipo de Publ: Carta
Responsável: BR1.1 - BIREME


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Id: biblio-1253101
Autor: Tsai, Wen-Che; Chang, Hung-Chi; Yin, Hsin-Yi; Huang, Meng-Chieh; Chandra Agrawal, Dinesh; Wen, Hsiao-Wei.
Título: The protective ability and cellular mechanism of Koelreuteria henryi Dummer flower extract against hydrogen peroxide-induced cellular oxidative damage
Fonte: Electron. j. biotechnol;47:89-99, sept. 2020. ilus, tab, graf.
Idioma: en.
Resumo: BACKGROUND: Koelreuteria henryi Dummer is an indigenous plant in Taiwan. The species has been used in traditional folk medicine for the promotion of liver functions and for treating malaria and urethritis. The present study investigated the antioxidant activity of the flower extract of Koelreuteria henryi Dummer. The extraction conditions were optimized by the contents of total phenolic acids and total flavonoids, and antioxidant activity assays. Moreover, an in vitro study for investigating antioxidant activity of K. henryi flower extract was demonstrated by hydrogen peroxide (H2O2)-induced apoptosis. RESULTS: K. henryi flower extracted for 150 min showed high contents of total phenolic acids and total flavonoids. In an in vitro model, L929 cells were pretreated with K. henryi flower extract, and then treated with H2O2 to induce oxidative damage. Results demonstrated that H2O2-induced apoptosis was inhibited by the treatment of 200 µg/ml K. henryi flower extract through the mitochondria-mediated pathway and mitogen-activated protein kinase (MAPK) pathway. The caspase 8/9 activity and expression of p-p38 and pERK were repressed by K. henryi flower extract. In addition, the prevention of H2O2-induced apoptosis by K. henryi flower extract activated the nuclear factor-erythroid 2-related factor (Nrf2) stress response pathway to transcript heme oxygenase 1 (HO-1). Also, K. henryi flower extract prevented H2O2-induced apoptosis through HO-1 production, as evident by the use of HO-1 inhibitor. CONCLUSIONS: The present study demonstrated that K. henryi flower extract could inhibit the H2O2-induced apoptosis in L929 cells through the activation of the Nrf2/HO-1 pathway.
Descritores: Extratos Vegetais/farmacologia
Estresse Oxidativo/efeitos dos fármacos
Sapindaceae/química
Antioxidantes/farmacologia
-Flavonoides/análise
Western Blotting
Apoptose
Flores/química
Heme Oxigenase-1
Fator 2 Relacionado a NF-E2
Caspase 8
Peróxido de Hidrogênio
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1002311
Autor: Castro, Micaele Maria Lopes; Martins, Maria Karolina Ferreira; Fagundes, Nathalia Carolina Fernandes; Nogueira, Bárbara Catarina Lima; da-Silva, Tarsila Guimarães Vieira; de-Oliveira, Fabio Reis; de-Souza-Rodrigues, Renata Duarte; Lima, Rafael Rodrigues.
Título: Ultrastructural Alterations and Physical Changes on Bovine Dentin After Internal Bleaching with 35 % Hydrogen Peroxide / Alteraciones Ultraestructurales y Cambios Físicos en la Dentina Bovina Después del Blanqueamiento Interior con un 35 % de Peróxido de Hidrógeno
Fonte: Int. j. odontostomatol. (Print);13(2):235-240, jun. 2019. tab, graf.
Idioma: en.
Resumo: ABSTRACT: The aim of the study was to investigate the effect of internal bleaching on physical proprieties and ultrastructure of the bovine dentin.40 bovine incisors were used, divided in four experimental groups: control group, composed by teeth that did not receive the bleaching agent (G1); teeth submitted to a single internal bleaching session (G2); teeth submitted to two internal bleaching sessions (G3); teeth submitted to three internal bleaching sessions (G4). In each of the sessions, 35 % hydrogen peroxide was applied for 45 minutes on the dentin surface. Tests were performed (microhardness and roughness) and were analyzed using one-way analysis of variance (ANOVA) and Tukey's post-hoc test (p≤0.05). Electromicrographs were captured for quality analysis. In the analysis of the superficial microhardness of the dentin, the internal bleaching reduced the Knoop microhardness since the first session, being observed statistically significant differences between the experimental groups and the control. The surface roughness gradually increased in the G2, G3 and G4 groups, but only G4 presented a statistically significant difference from the others. The qualitatively evaluated electromicrographs showed damage to the dentin ultrastructure, with areas of erosion and greater involvement of the intertubular when compared to peritubular dentin. Internal bleaching with 35 % hydrogen peroxide caused injuries in bovine dentin from the first treatment session. Both modifications in physical properties and dentin ultrastructure have been identified. These changes were intensified the higher the number of dentin internal bleaching sessions was exposed.

RESUMEN: El objetivo de este estudio fue investigar el efecto del blanqueamiento interno sobre las propiedades físicas y la ultraestructura de la dentina bovina. Se utilizaron 40 incisivos bovinos, divididos en cuatro grupos experimentales: grupo de control, compuesto por dientes que no recibieron el agente blanqueador (G1); dientes sometidos a una única sesión interna de blanqueamiento (G2); dientes sometidos a dos sesiones internas de blanqueamiento (G3); dientes sometidos a tres sesiones internas de blanqueamiento (G4). En cada una de las sesiones, se aplicó peróxido de hidrógeno al 35 % durante 45 minutos en la superficie de la dentina. Se realizaron pruebas (microdureza y rugosidad). Los datos se analizaron mediante análisis de varianza de una vía (ANOVA) y prueba post-hoc de Tukey (p≤0,05). Las electromicrografías fueron capturadas para el análisis cualitativo. En el análisis de la microdureza superficial de la dentina, el blanqueamiento interno redujo la microdureza de Knoop desde la primera sesión, observándose diferencias estadísticamente significativas entre los grupos experimentales y el control [NF2]. La rugosidad superficial aumentó gradualmente en los grupos G2, G3 y G4, pero solo G4 presentó una diferencia estadísticamente significativa con respecto a los otros [NF3]. Las electromicrografías evaluadas cualitativamente mostraron daño a la ultraestructura de la dentina, con áreas de erosión y una mayor participación de la dentina intertubular en comparación con la dentina peritubular. El blanqueamiento interno con peróxido de hidrógeno al 35 % causó lesiones en la dentina bovina en la primera sesión del tratamiento. Ambas modificaciones, en propiedades físicas y en la ultraestructura dentinaria, han sido identificadas. Estos cambios se intensificaron a medida que se expuso a mayor número de sesiones de blanqueamiento interno en la dentina.
Descritores: Clareamento Dental
Descoloração de Dente
Dentina/diagnóstico por imagem
-Padrões de Referência
Microscopia Eletrônica de Varredura
Análise Estatística
Análise de Variância
Esmalte Dentário
Estética Dentária
Clareadores Dentários
Peróxido de Hidrogênio
Limites: Animais
Bovinos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1278453
Autor: Gok, Ozlem; Beyaz, Seda; Aslan, Abdullah.
Título: Biological and Oxidative Effect of Ellagic Acid on Saccharomyces cerevisiae: A New Way for Culture Developing
Fonte: Braz. arch. biol. technol;64:e21210002, 2021. tab, graf.
Idioma: en.
Resumo: Abstract In this study, the effects of Ellagic acid (EA) on protein expression in yeasts and cellular development were investigated. Four groups were formed. Groups: 1) Control group; yeast only cultivated group; 2) Ellagic Acid (EA) group: EA (10%) given group; 3) Hydrogen peroxide (H2O2) Group: The group given H2O2 (15 mM); 4) EA + H2O2 group: EA (10%) + H2O2 (15 mM) group. After sterilization, EA (10%) and H2O2 (15 mM) were added to the Saccharomyces cerevisiae (S. cerevisiae) cultures and the cultures were grown at 30 °C for 1 hour, 3 hours, 5 hours and 24 hours (overnight). S. cerevisiae cell growth, lipid peroxidation MDA (malondialdehyde) analysis and GSH (glutathione) level were analyzed by spectrophotometer. Total protein changes were determined by SDS-PAGE electrophoresis and measured by the Bradford method. According to the obtained results, compared with the H2O2 group, cell development (1, 3, 5 and 24 hours), GSH level and total protein synthesis (24 hours) were increased with EA, while MDA level (24 hours) decreased. These results show that EA reduces oxidative damage, increases cell growth and it has a protective effect to promote protein synthesis in S. cerevisiae culture.
Descritores: Saccharomyces cerevisiae
Eletroforese em Gel de Poliacrilamida
Ácido Elágico
Peróxido de Hidrogênio
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-1254597
Autor: Ruiz Gamero, Claudia Andrea.
Título: Estabilidad del pH de cuatro geles a base de peróxido de hidrógeno en distintos intervalos de tiempo / PH stability of four hydrogen peroxide bleaching gels at different time intervals
Fonte: Rev. cient. odontol;9(2):e058, abr.-jun. 2021. ilus, tab.
Idioma: es.
Resumo: Objetivo: Comparar el pH de cuatro marcas de geles aclaradores a base de peróxido de hidrógeno de altas concentraciones (30%-35%) Whiteness HP Maxx (HPM), Lase Peroxide (LP), Whiteness HP Automixx (HPA)y Dash (DA), a través del tiempo de aplicación clínica (inicio, 15', 30' y 45'). Materiales y métodos: El estudio fue experimental in vitro. Se evaluaron 40 muestras (dientes bovinos) divididos en 4 grupos, uno para cada marca comercial de gel. Se preparó cada gel de acuerdo con las instrucciones del fabricante y se colocó una cantidad necesaria en la superficie vestibular; posteriormente, se registró el pH del gel con un pHmetro digital al inicio, 15, 30 y 45 minutos. Los datos se analizaron con las pruebas Anova, Friedman y Wilcoxon. Resultados: Hubo una tendencia a la disminución del pH desde el tiempo inicial de aplicación hasta el tiempo final, con excepción del grupo de la marca DA, el cual mostró que los valores del pH fueron aumentando a través del tiempo. En la marca HPM, existe una diferencia significativa entre el tiempo inicial de aplicación del gel y el resto de tiempos. En la marca LP, a partir de los 15' de aplicación, sí hay diferencia significativa entre los tiempos. En cuanto a la marca HPA, existieron diferencias significativas entre el tiempo inicial de aplicación y los demás tiempos. Finalmente, con la marca DA se encontró únicamente una diferencia significativa entre el tiempo inicial de aplicación y el tiempo final. Conclusiones: El pH de los geles aclaradores disminuyó a través del tiempo de aplicación clínica en todas las marcas estudiadas, con excepción de la marca Dash 30%, la cual mostró un aumento. (AU)

Objective: The aim of this in vitro study was to evaluate the pH of four bleaching agents based on high concentration hydrogen peroxide (30-35%) Whiteness HP Maxx (HPM), Lase Peroxide (LP), Whiteness HP Automixx (HPA) and Dash (DA) in different clinical periods (baseline, 15', 30' and 45'). Materials and methods: 40 specimens (bovine teeth) were divided into 4 groups; one group for each bleaching agent. Each bleaching agent was prepared according to the manufacturer's instructions and was applied on the vestibular surface. The pH of the bleaching agent was measured with a digital pH meter at baseline, 15, 30 and 45 minutes. ANOVA, Friedman and Wilcoxon tests were applied. Results: The pH values showed a trend to decreasing from the initial time of application to the final time, except for the DA group, which showed increasing pH values over time. The HPM group showed significant differences between baseline and the remaining periods. The LP group LP showed significant difference between 15' and the other periods. The HPA group showed significant differences between baseline and the remaining periods. Finally, the DA group, showed a significant difference between baseline and 45'. Conclusions: The pH values of 3 of the bleaching agents decreased over time, with the exception of Dash which increased in the different time periods. (AU)
Descritores: Clareamento Dental
Concentração de Íons de Hidrogênio
Peróxido de Hidrogênio
-Técnicas In Vitro
Epidemiologia Experimental
Tipo de Publ: Artigo Clássico
Responsável: PE1.1 - Oficina Universitária de Biblioteca


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Id: biblio-1135554
Autor: Hajilou, Samira; Zajkani, Elham; Naghili, Armaghan.
Título: Effect of a Resin Coating Material on the Microleakage of Class V Restorations With or Without Post-Operative Bleaching
Fonte: Pesqui. bras. odontopediatria clín. integr;20:e0015, 2020. tab.
Idioma: en.
Resumo: Abstract Objective: To evaluate the effect of a resin coating material on the microleakage of Class V composite resin restorations with and without post-operative bleaching. Material and Methods: Eighty class V cavities (3×3×1.5 mm) were prepared and filled with Z250 XT composite resin in the buccal and lingual surfaces of 40 sound human molars. Then the samples were randomly divided into 4 groups (n=20) and treated as follows: Group A: applying a resin coating (G-Coat Plus) on the restoration, then bleaching with 40% hydrogen peroxide; Group B: Post-Operative bleaching without prior use of resin coating; Group C: applying resin coating agent, and no further bleaching; and Group D: no resin coating, no bleaching. The specimens were thermocycled and immersed in 1% methylene blue for 24 hours, then cut into sections bucco-lingually. The samples were scored regarding the amount of dye penetration under a stereomicroscope (x20). Data were analyzed with Chi-squared and Fisher exact tests (p<0.05). Results: The maximum gingival and occlusal microleakage was detected in group B, while the minimum was seen in group C. In all the groups, microleakage at gingival margins was higher than occlusal margins (p<0.001). Conclusion: Application of a resin coating is an effective method in reducing microleakage of the restorations before and after bleaching.
Descritores: Resinas Compostas/química
Materiais Dentários
Peróxido de Hidrogênio/química
Dente Molar
-Técnicas In Vitro/métodos
Distribuição de Qui-Quadrado
Estatísticas não Paramétricas
Irã (Geográfico)/epidemiologia
Limites: Humanos
Masculino
Feminino
Tipo de Publ: Estudo Comparativo
Responsável: BR1264.1 - Biblioteca Setorial Prof Alberto M Campos


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Id: biblio-950746
Autor: Bae, Seunghee; Lim, Kyung Mi; Cha, Hwa Jun; An, In-Sook; Lee, Jeong Pyo; Lee, Kwang Sik; Lee, Ghang Tai; Lee, Kun Kook; Jung, Ho Jung; Ahn, Kyu Joong; An, Sungkwan.
Título: Arctiin blocks hydrogen peroxide-induced senescence and cell death though microRNA expression changes in human dermal papilla cells
Fonte: Biol. Res;47:1-11, 2014. graf, tab.
Idioma: en.
Projeto: Korean Health Technology R& D Project; . Ministry of Health & Welfare, Republic of Korea.
Resumo: BACKGROUND: Accumulating evidence indicates that reactive oxygen species (ROS) are an important etiological factor for the induction of dermal papilla cell senescence and hair loss, which is also known alopecia. Arctiin is an active lignin isolated from Arctium lappa and has anti-inflammation, anti-microbial, and anti-carcinogenic effects. In the present study, we found that arctiin exerts anti-oxidative effects on human hair dermal papilla cells (HHDPCs). RESULTS: To better understand the mechanism, we analyzed the level of hydrogen peroxide (H2O2)-induced cytotoxicity, cell death, ROS production and senescence after arctiin pretreatment of HHDPCs. The results showed that arctiin pretreatment significantly inhibited the H2O2-induced reduction in cell viability. Moreover, H2O2-induced sub-G1 phase accumulation and G2 cell cycle arrest were also downregulated by arctiin pretreatment. Interestingly, the increase in intracellular ROS mediated by H2O2 was drastically decreased in HHDPCs cultured in the presence of arctiin. This effect was confirmed by senescence associated-beta galactosidase (SA-ß-gal) assay results; we found that arctiin pretreatment impaired H2O2-induced senescence in HHDPCs. Using microRNA (miRNA) microarray and bioinformatic analysis, we showed that this anti-oxidative effect of arctiin in HHDPCs was related with mitogen-activated protein kinase (MAPK) and Wnt signaling pathways. CONCLUSIONS: Taken together, our data suggest that arctiin has a protective effect on ROS-induced cell dysfunction in HHDPCs and may therefore be useful for alopecia prevention and treatment strategies.
Descritores: Envelhecimento/metabolismo
Espécies Reativas de Oxigênio/antagonistas & inibidores
Folículo Piloso/efeitos dos fármacos
MicroRNAs/metabolismo
Furanos/farmacologia
Glucosídeos/farmacologia
-Envelhecimento/efeitos dos fármacos
Regulação para Baixo/efeitos dos fármacos
Regulação para Cima/efeitos dos fármacos
Linhagem Celular
Sobrevivência Celular/efeitos dos fármacos
Morte Celular/efeitos dos fármacos
beta-Galactosidase/análise
Folículo Piloso/citologia
Folículo Piloso/metabolismo
Derme/citologia
Derme/efeitos dos fármacos
Derme/metabolismo
Análise de Sequência com Séries de Oligonucleotídeos
MicroRNAs/efeitos dos fármacos
Pontos de Checagem do Ciclo Celular/efeitos dos fármacos
Peróxido de Hidrogênio/farmacologia
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central



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