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Id: biblio-1055406
Autor: Department of BotanyPrajapati, Rajesh; Department of BotanyYadav, Shivam; Department of BotanyMitra, Sonali; Department of BotanyRai, Priya; Department of BotanyMishra, Rajeev; Department of BotanyAtri, Neelam.
Título: Genome-Wide Assessment of Putative Superoxide Dismutases in Unicellular and Filamentous Cyanobacteria
Fonte: Braz. arch. biol. technol;62:e19170747, 2019. tab, graf.
Idioma: en.
Resumo: Abstract Cyanobacteria are photoautotrophic prokaryotes capable to grow in diverse ecological habitats, originated 2.5-3.5 billion years ago and were first to produce oxygen. Since then superoxide dismutases (SOD) acquired great significance due to their ability to catalyze detoxification of byproducts of oxygenic photosynthesis i.e. superoxide radicals. In the present study, we extracted information regarding SODs from species of sequenced cyanobacteria and investigated their diversity, conservation, domain structure, and evolution. 144 putative SOD homologs were identified. Unlike other protein families (ex. serine-threonine kinases) SODs are present in all cyanobacterial species reflecting their significant role in survival. However, their distribution varies fewer (0.01%-0.09%) found in unicellular marine strains whereas abundant (0.02%-0.07%) in filamentous nitrogen-fixing cyanobacteria. They were classified into three major subfamilies according to their domain structures: Fe/MnSOD, Cu/ZnSOD and NiSOD. Interestingly, they lack additional domains as found in proteins of other families however motifs and invariant amino acids typical in eukaryotic SODs were conserved well in these proteins indicating similar catalytic mechanism as eukaryotic SODs. Phylogenetic relationships correspond well with phylogenies based on 16S rRNA and clustering occurs on the basis of structural characteristics such as domain organization. Gene gain-and-loss is insignificant during SOD evolution as evidenced by the absence of additional domain. This study has not only examined an overall background of sequence-structure-function interactions for the SOD gene family but also revealed variation among SOD distribution based on ecophysiological and morphological characters.
Descritores: Superóxido Dismutase
Superóxidos
-Filogenia
Genômica
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: biblio-950857
Autor: Liu, Yu; Wang, Lu; Liu, Heng; Zhao, Rongrong; Liu, Bin; Fu, Quanjuan; Zhang, Yuanhu.
Título: The antioxidative defense system is involved in the premature senescence in transgenic tobacco (Nicotiana tabacum NC89)
Fonte: Biol. Res;49:1-5, 2016. ilus, graf, tab.
Idioma: en.
Projeto: National Natural Science Foundation of China.
Resumo: BACKGROUND: α-Farnesene is a volatile sesquiterpene synthesized by the plant mevalonate (MVA) pathway through the action of α-farnesene synthase. The α-farnesene synthase 1 (MdAFS1) gene was isolated from apple peel (var. white winterpearmain), and transformed into tobacco (Nicotiana tabacum NC89). The transgenic plants had faster stem elongation during vegetative growth and earlier flowering than wild type (WT). Our studies focused on the transgenic tobacco phenotype. RESULTS: The levels of chlorophyll and soluble protein decreased and a lower seed biomass and reduced net photosynthetic rate (Pn) in transgenic plants. Reactive oxygen species (ROS) such as hydrogen peroxide (H2O2) and superoxide radicals (O2._) had higher levels in transgenics compared to controls. Transgenic plants also had enhanced sensitivity to oxidative stress. The transcriptome of 8-week-old plants was studied to detect molecular changes. Differentially expressed unigene analysis showed that ubiquitin-mediated proteolysis, cell growth, and death unigenes were upregulated. Unigenes related to photosynthesis, antioxidant activity, and nitrogen metabolism were downregulated. Combined with the expression analysis of senescence marker genes, these results indicate that senescence started in the leaves of the transgenic plants at the vegetative growth stage. CONCLUSIONS: The antioxidative defense system was compromised and the accumulation of reactive oxygen species (ROS) played an important role in the premature aging of transgenic plants.
Descritores: Tabaco/fisiologia
Plantas Geneticamente Modificadas/fisiologia
Antioxidantes/fisiologia
-Fotossíntese/fisiologia
Sesquiterpenos/análise
Sesquiterpenos/metabolismo
Fatores de Tempo
Tabaco/genética
Marcadores Genéticos
Expressão Gênica/fisiologia
Plantas Geneticamente Modificadas/genética
Espécies Reativas de Oxigênio/análise
Espécies Reativas de Oxigênio/metabolismo
Superóxidos/análise
Superóxidos/metabolismo
Folhas de Planta/fisiologia
Estresse Oxidativo/fisiologia
Regulação da Expressão Gênica de Plantas/fisiologia
Reação em Cadeia da Polimerase em Tempo Real
Peróxido de Hidrogênio/análise
Peróxido de Hidrogênio/metabolismo
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-840076
Autor: Jordão, Camila P; Fernandes, Tiago; Tanaka, Leonardo Yuji; Bechara, Luiz R. Grassmann; de Sousa, Luis Gustavo Oliveira; Oliveira, Edilamar M; Ramires, Paulo Rizzo.
Título: Aerobic Swim Training Restores Aortic Endothelial Function by Decreasing Superoxide Levels in Spontaneously Hypertensive Rats
Fonte: Clinics;72(5):310-316, May 2017. graf.
Idioma: en.
Resumo: OBJECTIVE: We aimed to determine whether aerobic training decreases superoxide levels, increases nitric oxide levels, and improves endothelium-dependent vasodilation in the aortas of spontaneously hypertensive rats. METHODS: Spontaneously hypertensive rats (SHR) and Wistar Kyoto rats (WKY) were distributed into 2 groups: sedentary (SHRsd and WKYsd, n=10 each) and swimming-trained (SHRtr, n=10 and WKYtr, n=10, respectively). The trained group participated in training sessions 5 days/week for 1 h/day with an additional work load of 4% of the animal’s body weight. After a 10-week sedentary or aerobic training period, the rats were euthanized. The thoracic aortas were removed to evaluate the vasodilator response to acetylcholine (10-10 to 10-4 M) with or without preincubation with L-NG-nitro-L-arginine methyl ester hydrochloride (L-NAME; 10-4 M) in vitro. The aortic tissue was also used to assess the levels of the endothelial nitric oxide synthase and nicotinamide adenine dinucleotide oxidase subunit isoforms 1 and 4 proteins, as well as the superoxide and nitrite contents. Blood pressure was measured using a computerized tail-cuff system. RESULTS: Aerobic training significantly increased the acetylcholine-induced maximum vasodilation observed in the SHRtr group compared with the SHRsd group (85.9±4.3 vs. 71.6±5.2%). Additionally, in the SHRtr group, superoxide levels were significantly decreased, nitric oxide bioavailability was improved, and the levels of the nicotinamide adenine dinucleotide oxidase subunit isoform 4 protein were decreased compared to the SHRsd group. Moreover, after training, the blood pressure of the SHRtr group decreased compared to the SHRsd group. Exercise training had no effect on the blood pressure of the WKYtr group. CONCLUSIONS: In SHR, aerobic swim training decreased vascular superoxide generation by nicotinamide adenine dinucleotide oxidase subunit isoform 4 and increased nitric oxide bioavailability, thereby improving endothelial function.
Descritores: Aorta Torácica/fisiopatologia
Endotélio Vascular/fisiopatologia
Hipertensão/fisiopatologia
Condicionamento Físico Animal/fisiologia
Superóxidos/análise
Natação/fisiologia
-Western Blotting
Etídio/análogos & derivados
Teste de Esforço
Fluorescência
Hemodinâmica
NAD/análise
NG-Nitroarginina Metil Éster/análise
NG-Nitroarginina Metil Éster/metabolismo
Óxido Nítrico Sintase Tipo III/análise
Óxido Nítrico Sintase Tipo III/metabolismo
Nitritos/análise
Nitritos/metabolismo
Distribuição Aleatória
Ratos Endogâmicos SHR
Valores de Referência
Reprodutibilidade dos Testes
Superóxidos/metabolismo
Fatores de Tempo
Vasodilatação/fisiologia
Limites: Animais
Masculino
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: biblio-839250
Autor: Horst, A; de Souza, JA; Santos, MCQ; Riffel, APK; Kolberg, C; Ribeiro, MFM; de Fraga, LS; Partata, WA.
Título: N-acetylcysteine downregulates phosphorylated p-38 expression but does not reverse the increased superoxide anion levels in the spinal cord of rats with neuropathic pain
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;50(2):e5801, 2017. graf.
Idioma: en.
Resumo: We determined the effect of N-acetylcysteine (NAC) on the expression of the phosphorylated p38 (p-p38) protein and superoxide anion generation (SAG), two important players in the processing of neuropathic pain, in the lumbosacral spinal cord of rats with chronic constriction injury (CCI)-induced neuropathic pain. The sciatic functional index (SFI) was also measured to assess the functional recovery post-nerve lesion. Thirty-six male Wistar rats were divided equally into the following groups: Naive (rats did not undergo surgical manipulation); Sham (rats in which all surgical procedures involved in CCI were used except the ligature), and CCI (rats in which four ligatures were tied loosely around the right common sciatic nerve), which received 2, 4, or 8 intraperitoneal injections of NAC (150 mg·kg-1·day-1) or saline beginning 4 h after CCI. Rats were sacrificed 1, 3, and 7 days after CCI. The SFI was measured on these days and the lumbosacral spinal cord was used for analysis of p-p38 expression and SAG. CCI induced a decrease in SFI as well as an increase in p-p38 expression and SAG in the spinal cord. The SFI showed a partial recovery at day 7 in saline-treated CCI rats, but recovery was improved in NAC-treated CCI rats. NAC induced a downregulation in p-p38 expression at all time-points evaluated, but did not reverse the increased SAG induced by CCI. Since p-p38 is a mediator in neuropathic pain and/or nerve regeneration, modulation of this protein may play a role in NAC-induced effects in CCI rats.
Descritores: Acetilcisteína/uso terapêutico
Neuralgia/tratamento farmacológico
Proteínas Quinases p38 Ativadas por Mitógeno/efeitos dos fármacos
Medula Espinal/efeitos dos fármacos
Superóxidos/metabolismo
-Western Blotting
Constrição Patológica
Modelos Animais de Doenças
Regulação para Baixo/efeitos dos fármacos
Neuralgia/etiologia
Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo
Limiar da Dor
Fosforilação/efeitos dos fármacos
Ratos Wistar
Medula Espinal/metabolismo
Limites: Animais
Masculino
Ratos
Responsável: BR1.1 - BIREME


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Id: lil-748557
Autor: Rosario, Andre Loureiro.
Título: Efeito da ressuscitação volêmica precoce na resposta inflamatória e no estresse oxidativo cardiovascular do choque séptico experimental / SvO2 guided resuscitation for experimental septic shock: effects of fluid infusion and dobutamine on hemodynamics, inflammatory response and cardiovascular oxidative stress.
Fonte: São Paulo; s.n; 2014. [95] p. ilus, graf, tab.
Idioma: pt.
Tese: Apresentada a Universidade de São Paulo. Faculdade de Medicina para obtenção do grau de Doutor.
Resumo: Os mecanismos fisiopatológicos associados aos efeitos benéficos da reanimação guiada pela saturação venosa mista de oxigênio (SvO2) durante a sepse não são claros. Nosso objetivo foi avaliar os efeitos de um algoritmo de reanimação guiado pela SvO2 incluindo fluidos, noradrenalina e dobutamina na hemodinâmica, resposta inflamatória e estresse oxidativo cardiovascular durante um modelo experimental que se assemelha clinicamente ao choque séptico. Dezoito porcos anestesiados e cateterizados (35-45 kg) foram submetidos à peritonite por inoculação fecal (0,75 g/Kg). Depois de permanecerem hipotensos, antibióticos foram administrados e os animais foram randomizados em dois grupos: controle (n=9), com suporte hemodinâmico visando pressão venosa central de 8-12 mmHg, débito urinário de 0,5 ml/kg por hora, e pressão arterial média acima de 65 mmHg; e grupo SvO2 (n=9), com os objetivos acima referidos, além de SvO2 acima de 65%. As intervenções duraram 12 hs e incluíram Ringer Lactato e norepinefrina (ambos os grupos) e dobutamina (grupo SvO2). A resposta inflamatória foi avaliada pela concentração plasmática de citocinas, expressão de CD14 de neutrófilos, geração de espécies reativas de oxigênio e apoptose. O estresse oxidativo foi avaliado pelas concentrações de nitratos no miocárdio e no plasma, a atividade miocárdica e vascular de NAD(P)H oxidase, conteúdo de glutationa do miocárdio e expressão de nitrotirosina. A reanimação guiada por SvO2 foi associada com melhor índice sistólico, oferta de oxigênio e diurese. A sepse induziu em ambos os grupos um aumento significativo na concentração de IL-6, nas concentrações de nitrato de plasma e diminuição persistente na expressão de CD14 em neutrófilos. A apoptose e a geração de espécies reativas de oxigênio por neutrófilos não foram diferentes entre os grupos. As estratégias de tratamento não alteraram significativamente os parâmetros de estresse oxidativo. Assim, uma abordagem destinada a otimizar a SvO2...

The pathogenetic mechanisms associated to the beneficial effects of mixed venous oxygen saturation (SvO2)-guided resuscitation during sepsis are unclear. Our purpose was to evaluate the effects of an algorithm of SvO2-driven resuscitation including fluids, norepinephrine and dobutamine on hemodynamics, inflammatory response and cardiovascular oxidative stress during a clinically resembling experimental model of septic shock. Eighteen anesthetized and catheterized pigs (35-45 Kg) were submitted to peritonitis by fecal inoculation (0.75 g/Kg). After hypotension, antibiotics were administered, and the animals were randomized to two groups: control (n=9), with hemodynamic support aiming central venous pressure 8 to 12 mmHg, urinary output 0.5 ml/Kg per hour, and mean arterial pressure greater than 65 mmHg; and group SvO2 (n =9), with the goals above, plus SvO2 greater than 65%. The interventions lasted 12 h, and lactated Ringer's and norepinephrine (both groups) and dobutamine (SvO2 group) were administered. Inflammatory response was evaluated by plasma concentration of cytokines, neutrophil CD14 expression, oxidant generation, and apoptosis. Oxidative stress was evaluated by plasma and myocardial nitrate concentrations, myocardial and vascular NAD(P)H oxidase activity, myocardial glutathione content, and nitrotyrosine expression. Mixed venous oxygen saturation-driven resuscitation was associated with improved systolic index, oxygen delivery, and diuresis. Sepsis induced in both groups a significant increase on IL-6 concentrations and plasma nitrate concentrations and persistent decrease in neutrophil CD14 expression. Apoptosis rate and neutrophil oxidant generation were not different between groups. Treatment strategies did not significantly modify oxidative stress parameters. Thus, an approach aiming SvO2 during sepsis improves hemoynamics, without any significant effect on inflammatory response and oxidative stress. The beneficial effects...
Descritores: Citocinas
Radicais Livres
Hemodinâmica
Modelos Animais
Óxido Nítrico
Estresse Oxidativo
Sepse
Choque Séptico
Superóxidos
Síndrome de Resposta Inflamatória Sistêmica
Limites: Animais
Masculino
Feminino
Responsável: BR66.1 - Divisão de Biblioteca e Documentação


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Id: lil-748281
Autor: Sette, Luís Henrique Bezerra Cavalcanti; Lopes, Edmundo Pessoa de Almeida.
Título: The reduction of serum aminotransferase levels is proportional to the decline of the glomerular filtration rate in patients with chronic kidney disease
Fonte: Clinics;70(5):346-349, 05/2015. tab, graf.
Idioma: en.
Resumo: OBJECTIVE: This study sought to determine the serum aminotransferase levels of patients with predialysis chronic kidney disease and establish their relationships with serum creatinine levels and glomerular filtration rate. METHODS: Patients with chronic kidney disease were evaluated between September 2011 and May 2012. Aminotransferase and creatinine serum levels were measured using an automated kinetic method, and glomerular filtration rates were estimated using the Cockroft-Gault and Modification of Diet in Renal Disease formulas to classify patients into chronic kidney disease stages. RESULTS: Exactly 142 patients were evaluated (mean age: 64±16 years). The mean creatinine serum level and glomerular filtration rate were 3.3±1.2 mg/dL and 29.1±13 mL/min/1.73 m2, respectively. Patients were distributed according to their chronic kidney disease stages as follows: 3 (2.1%) patients were Stage 2; 54 (38%) were Stage 3; 70 (49.3%) were Stage 4; and 15 (10.5%) were Stage 5. The mean aspartate aminotransferase and alanine aminotransferase serum levels showed a reduction in proportion to the increase in creatinine levels (p=0.001 and p=0.05, respectively) and the decrease in glomerular filtration rate (p=0.007 and p=0.028, respectively). Alanine aminotransferase and aspartate aminotransferase serum levels tended to be higher among patients classified as stage 2 or 3 compared with those classified as stage 4 or 5 (p=0.08 and p=0.06, respectively). CONCLUSIONS: The aspartate aminotransferase and alanine aminotransferase serum levels of patients with predialysis chronic kidney disease decreased in proportion to the progression of the disease; they were negatively correlated with creatinine levels and directly correlated with glomerular filtration rate. .
Descritores: Poluentes Ambientais/toxicidade
Prepúcio do Pênis/efeitos dos fármacos
Queratinócitos/efeitos dos fármacos
Bifenilos Policlorados/toxicidade
Telomerase/metabolismo
Encurtamento do Telômero/efeitos dos fármacos
-Técnicas de Cultura de Células
Linhagem Celular
Ciclo Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
DNA
Relação Dose-Resposta a Droga
Ativação Enzimática
Prepúcio do Pênis/enzimologia
Prepúcio do Pênis/ultraestrutura
Queratinócitos/enzimologia
Queratinócitos/ultraestrutura
Estresse Oxidativo/efeitos dos fármacos
Superóxidos/metabolismo
Encurtamento do Telômero/genética
Limites: Humanos
Masculino
Tipo de Publ: Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-745885
Autor: Guimarães-Souza, Nadia Karina; Yamaleyeva, Liliya Marsovna; Lu, Baisong; Ramos, Ana Claudia Mallet de Souza; Bishop, Colin Edward; Andersson, Karl Erik.
Título: Superoxide overproduction and kidney fibrosis: a new animal model / Produção aumentada de superóxido e fibrose renal: novo modelo animal
Fonte: Einstein (Säo Paulo);13(1):79-88, Jan-Mar/2015. graf.
Idioma: en.
Resumo: Objective To establish whether the mutation in the Immp2L gene induces renal fibrosis and whether aging exacerbates renal morphology in mice. Methods Female mutant mice with mutation in the inner mitochondrial membrane peptidase 2-like protein at 3 and 18 months of age were used. Renal fibrosis was analyzed using classic fibrosis score, Masson’s trichrome staining, and analysis of profibrotic markers using real time polymerase chain reaction (superoxide dismutase 1, metalloproteinase-9, erythropoietin, transforming growth factor beta), and immunostaining (fibroblasts and Type IV collagen). Oxidative stress markers were determined by immunohistochemistry. The number of renal apoptotic cells was determined. Renal function was estimated by serum creatinine. Results Young mutant mice had significantly more glomerulosclerosis than age-matched mice (p=0.034). Mutant mice had more tubular casts (p=0.025), collagen deposition (p=0.019), and collagen type IV expression (p<0.001). Superoxide dismutase 1 expression was significantly higher in young mutants (p=0.038). Old mutants exhibited significantly higher expression of the fibroblast marker and macrophage marker (p=0.007 and p=0.012, respectively). The real time polymerase chain reaction of metalloproteinase-9 and erythropoietin were enhanced 2.5- and 6-fold, respectively, in old mutants. Serum creatinine was significantly higher in old mutants (p<0.001). Conclusion This mutation altered renal architecture by increasing the deposition of extracellular matrix, oxidative stress, and inflammation, suggesting a protective role of Immp2L against renal fibrosis. .

Objetivo Estabelecer se a mutação no gene Immp2L induz à fibrose renal e se o envelhecimento exacerba a morfologia renal em camundongos. Métodos Foram usadas fêmeas de camundongos mutantes para proteína semelhante à peptidase 2 da camada interna da mitocôndria, com 3 e 18 meses de idade. Para analisar a fibrose renal, foram usados o escore clássico de fibrose, a coloração com tricrômio de Masson, e a análise de marcadores profibróticos, por meio da reação em cadeia de polimerase em tempo real (superóxido dismutase 1, metalonoproteinase-9, eritropoietina e fator transformador de crescimento beta), e a imunocoloração (fibroblastos e colágeno IV). Marcadores de estresse oxidativo foram determinados por imuno-histoquímica. O número de células apoptóticas renais foi analisado. A função renal foi estimada por creatinina sérica. Resultados Camundongos mutantes jovens apresentaram glomeruloesclerose em quantidade significativamente maior que animais da mesma idade (p=0,034). Os mutantes mostraram maior formação de cilindros tubulares (p=0,025), deposição de colágeno (p=0,019) e maior expressão de colágeno do tipo IV (p<0,001). A expressão de superóxido dismutase 1 foi maior em mutantes jovens (p=0,038). Mutantes idosas exibiram maior expressão dos marcadores de fibroblastos e macrófagos (p=0,007 e p=0,012, respectivamente). As reações da cadeia de polimerase em tempo real da metalanoproteinase-9 e da eritropoietina estavam aumentadas em 2,5 e 6 vezes, respectivamente, em mutantes idosas. A creatinina sérica foi significantemente maior em animais idosos mutantes (p<0,001). Conclusão Essa mutação alterou a arquitetura renal pelo aumento da deposição de matriz extracelular, estresse oxidativo e inflamação, sugerindo papel de proteção de Immp2L contra a fibrose renal. .
Descritores: Modelos Animais de Doenças
Endopeptidases/genética
Endopeptidases/metabolismo
Rim/metabolismo
Rim/patologia
Mutação/fisiologia
Superóxidos/metabolismo
-Apoptose/genética
Apoptose/fisiologia
Colágeno/análise
Creatinina/sangue
Eritropoetina/análise
Fibrose/genética
Fibrose/metabolismo
Metaloproteinase 9 da Matriz/análise
Estresse Oxidativo/genética
Estresse Oxidativo/fisiologia
Reação em Cadeia da Polimerase em Tempo Real
Insuficiência Renal Crônica/genética
Insuficiência Renal Crônica/metabolismo
Superóxido Dismutase/análise
Superóxidos/análise
Fator de Crescimento Transformador beta/análise
Limites: Animais
Feminino
Camundongos
Responsável: BR1.1 - BIREME


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Id: lil-744363
Autor: Maioli, N.A.; Zarpelon, A.C.; Mizokami, S.S.; Calixto-Campos, C.; Guazelli, C.F.S.; Hohmann, M.S.N.; Pinho-Ribeiro, F.A.; Carvalho, T.T.; Manchope, M.F.; Ferraz, C.R.; Casagrande, R.; Verri Jr, W.A..
Título: The superoxide anion donor, potassium superoxide, induces pain and inflammation in mice through production of reactive oxygen species and cyclooxygenase-2
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;48(4):321-331, 4/2015. graf.
Idioma: en.
Resumo: It is currently accepted that superoxide anion (O2•−) is an important mediator in pain and inflammation. The role of superoxide anion in pain and inflammation has been mainly determined indirectly by modulating its production and inactivation. Direct evidence using potassium superoxide (KO2), a superoxide anion donor, demonstrated that it induced thermal hyperalgesia, as assessed by the Hargreaves method. However, it remains to be determined whether KO2 is capable of inducing other inflammatory and nociceptive responses attributed to superoxide anion. Therefore, in the present study, we investigated the nociceptive and inflammatory effects of KO2. The KO2-induced inflammatory responses evaluated in mice were: mechanical hyperalgesia (electronic version of von Frey filaments), thermal hyperalgesia (hot plate), edema (caliper rule), myeloperoxidase activity (colorimetric assay), overt pain-like behaviors (flinches, time spent licking and writhing score), leukocyte recruitment, oxidative stress, and cyclooxygenase-2 mRNA expression (quantitative PCR). Administration of KO2 induced mechanical hyperalgesia, thermal hyperalgesia, paw edema, leukocyte recruitment, the writhing response, paw flinching, and paw licking in a dose-dependent manner. KO2 also induced time-dependent cyclooxygenase-2 mRNA expression in the paw skin. The nociceptive, inflammatory, and oxidative stress components of KO2-induced responses were responsive to morphine (analgesic opioid), quercetin (antioxidant flavonoid), and/or celecoxib (anti-inflammatory cyclooxygenase-2 inhibitor) treatment. In conclusion, the well-established superoxide anion donor KO2 is a valuable tool for studying the mechanisms and pharmacological susceptibilities of superoxide anion-triggered nociceptive and inflammatory responses ranging from mechanical and thermal hyperalgesia to overt pain-like behaviors, edema, and leukocyte recruitment.
Descritores: /efeitos dos fármacos
CYCLOOXYGENASE TEMEFOS/efeitos dos fármacos
Hiperalgesia/induzido quimicamente
Inflamação/induzido quimicamente
Dor Nociceptiva/induzido quimicamente
Superóxidos/farmacologia
-Analgésicos Opioides/uso terapêutico
Antioxidantes/uso terapêutico
/uso terapêutico
CYCLOOXYGENASE TEMEFOS INHIBITORS/uso terapêutico
/genética
CYCLOOXYGENASE TEMEFOS/genética
Edema/induzido quimicamente
Membro Posterior
Temperatura Alta
Hiperalgesia/tratamento farmacológico
Inflamação/tratamento farmacológico
Dor Nociceptiva/tratamento farmacológico
Medição da Dor/métodos
Peroxidase/efeitos dos fármacos
Reação em Cadeia da Polimerase em Tempo Real
Espécies Reativas de Oxigênio/metabolismo
Pele/efeitos dos fármacos
Fatores de Tempo
Transcrição Genética/efeitos dos fármacos
Limites: Animais
Masculino
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-726605
Autor: Alarcón, David; Paredes, Marco; Ramos, Daniela; González, Katerina; Díaz, Ramiro; Núñez, Daniela.
Título: Los extractos acuoso y metanólico de Berberis darwinii H. (Berberidaceae) inhiben respuestas celulares innatas en monocitos humanos tratados in vitro / Aqueous and methanol extracts of Berberis darwinii H. (Berberidaceae) inhibit innate celular responses in human monocytes in vitro treated
Fonte: Bol. latinoam. Caribe plantas med. aromát;13(1):81-91, ene. 2014. ilus, tab.
Idioma: es.
Projeto: CORFO. INNOVA Chile.
Resumo: Berberis darwinii H is a native plant of South America, popularly referred to Michay. This species has historically been used by indigenous cultures of Chile as medicinal herb. To preliminarily assess their anti-inflammatory effects was investigated the aqueous and methanolic root extract this plant in human monocytes. The results indicated that the extracts inhibit the production of superoxide anion, the expression of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1-beta (IL-1beta) in monocytes activated with lipopolysaccharide. This result suggests the existence of compounds with potential anti-inflammatory action in these extracts.

Berberis darwinii H. es una planta nativa de América del Sur, conocida popularmente como Michay. Esta especie ha sido históricamente utilizada por las culturas indígenas de Chile como hierba medicinal. Con el fin de evaluar preliminarmente sus efectos anti-inflamatorios, se investigaron dos tipos de extractos; metanólico y acuoso, preparados a partir de la raíz de esta planta. Los resultados indican que estos extractos inhiben la producción de anión superóxido, la expresión del factor de necrosis tumoral-alfa (TNF-alfa) y de interleucina-1beta, (IL-1beta) en monocitos activados con lipopolisacárido. Estos resultados sugieren la existencia de compuestos con potencial acción antiinflamatoria en esta planta.
Descritores: Anti-Inflamatórios
Berberis/química
Extratos Vegetais/farmacologia
Monócitos
-Sobrevivência Celular
Interleucina-1beta
Metanol
Raízes de Plantas/química
Superóxidos
Testes de Toxicidade
Fator de Necrose Tumoral alfa
Limites: Humanos
Responsável: CL1.1 - Biblioteca Central


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Id: lil-696898
Autor: Rocha, Ricelli Endrigo Ruppel; Coelho, Isabela; Pequito, Daniela Cristina T.; Yamagushi, Adriana; Borghetti, Gina; Yamazaki, Ricardo Key; Brito, Gleisson Alisson Pereira de; Machado, Juliano; Kryczyk, Marcelo; Nunes, Everson Araújo; Venera, Graciela; Fernandes, Luiz Claudio.
Título: Interval training attenuates the metabolic disturbances in type 1 diabetes rat model / Treinamento intervalado atenua os distúrbios metabólicos em modelo de ratos diabéticos do tipo 1
Fonte: Arq. bras. endocrinol. metab;57(8):594-602, Nov. 2013. graf, tab.
Idioma: en.
Resumo: OBJECTIVE: This study investigated the effect of interval training on blood biochemistry and immune parameters in type 1 diabetic rats. MATERIALS AND METHODS: Male Wistar rats were divided into four groups: sedentary (SE, n = 15), interval training (IT, n = 17), diabetic sedentary (DSE, n = 17), diabetic interval training (DIT, n = 17). Diabetes was induced by i.v. injection of streptozotocin (60 mg/kg). Swimming Interval Training consisted of 30-s exercise with 30-s rest, for 30 minutes, during 6 weeks, four times a week, with an overload of 15% of body mass. Plasma glucose, lactate, triacylglycerol and total cholesterol concentrations, phagocytic capacity, cationic vesicle content, and superoxide anion and hydrogen peroxide production by blood neutrophils and peritoneal macrophages were evaluated. Proliferation of mesenteric lymphocytes was also estimated. RESULTS: Interval training resulted in attenuation of the resting hyperglycemic state and decreased blood lipids in the DIT group. Diabetes increased the functionality of blood neutrophils and peritoneal macrophages in the DSE group. Interval training increased all functionality parameters of peritoneal macrophages in the IT group. Interval training also led to a twofold increase in the proliferation of mesenteric lymphocytes after 6 weeks of exercise in the DIT group. CONCLUSION: Low-volume high-intensity physical exercise attenuates hyperglycemia and dislipidemia induced by type 1 diabetes, and induces changes in the functionality of innate and acquired immunity.

OBJETIVO: Este estudo investigou os efeitos do treinamento intervalado sobre parâmetros bioquímicos e imunológicos em ratos diabéticos do tipo 1. MATERIAIS E MÉTODOS: Ratos Wistar machos foram divididos em quatro grupos: sedentário (SE, n = 15), treinamento intervalado (TI, n = 17), sedentário diabético (SED, n = 17) e treinamento intervalado diabético (TID, n = 17). O diabetes foi induzido por uma injeção intravenosa de estreptozotocina (60 mg/kg). O treinamento intervalado de natação consistiu de 30s de exercício com 30s de recuperação, 30 minutos, durante 6 semanas, 4 vezes por semana, com sobrecarga de 15% da massa corporal. Foram avaliados glicemia, lactato sanguíneo, concentração de triacilglicerol e colesterol total, capacidade fagocítica, conteúdo de vesículas catiô­nicas, produção de ânion superóxido e peróxido de hidrogênio por neutrófilos sanguíneos e macrófagos peritoneais. A proliferação de linfócitos mesentéricos também foi avaliada. RESULTADOS: O treinamento intervalado resultou em atenuação do estado hiperglicêmico e diminuiu os lipídeos sanguíneos no grupo TID. O diabetes aumentou a funcionalidade dos neutrófilos sanguíneos e macrófagos peritoneais do grupo SED. O treinamento intervalado aumentou todos os parâmetros funcionais dos macrófagos peritoneais do grupo TI. O treinamento intervalado também aumentou duas vezes a proliferação dos linfócitos mesentéricos após seis semanas de exercício do grupo TID. CONCLUSÃO: O treinamento intervalado atenua a hiperglicemia e a dislipidemia induzida pelo diabetes do tipo 1 e induz mudanças na funcionalidade da imunidade inata e adquirida.
Descritores: Diabetes Mellitus Experimental/induzido quimicamente
Diabetes Mellitus Experimental/metabolismo
Diabetes Mellitus Tipo 1/metabolismo
Dislipidemias/etiologia
Hiperglicemia/etiologia
Condicionamento Físico Animal/métodos
-Biomarcadores
Glicemia/metabolismo
Proliferação de Células
Modelos Animais de Doenças
Diabetes Mellitus Tipo 1/complicações
Peróxido de Hidrogênio/metabolismo
Neutrófilos/metabolismo
Fagocitose/fisiologia
Ratos Wistar
Comportamento Sedentário
Estreptozocina/farmacologia
Superóxidos/metabolismo
Limites: Animais
Masculino
Responsável: BR1.1 - BIREME



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