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Id: biblio-1001185
Autor: Ayar, Ganime; Sahin, Sanliay; Men Atmaca, Yasemin; Uysal Yazici, Mutlu; Neselioglu, Salim; Erel, Ozcan.
Título: La homeostasis de tiol/disulfuro es un indicador del estrés oxidativo en los niños críticamente enfermos con sepsis / Thiol-disulphide homeostasis is an oxidative stress indicator in critically ill children with sepsis
Fonte: Arch. argent. pediatr;117(3):143-148, jun. 2019. ilus, tab.
Idioma: en; es.
Resumo: Objetivo. Evaluar un novedoso marcador del estrés oxidativo (la homeostasis de tiol /disulfuro) en la sepsis pediátrica y determinar sus efectos sobre el pronóstico de esta afección. Métodos. En el estudio, se incluyeron pacientes con diagnóstico de sepsis y controles sanos. Se midieron las concentraciones de tiol total, tiol nativo, disulfuro, disulfuro /tiol total, disulfuro /tiol nativo y tiol nativo/tiol total en los grupos con sepsis y de referencia. Se compararon los parámetros entre los supervivientes y los no supervivientes del grupo con sepsis. Se midieron las concentraciones de hemoglobina, leucocitos, trombocitos, lactato y proteína C-reactiva en los pacientes con sepsis al momento del diagnóstico. Se utilizaron el puntaje de riesgo de mortalidad pediátrico (Pediatric Risk of Mortality, PRISM) y el puntaje de disfunción orgánica (Pediatric Logistic Organ Dysfunction, PELOD) para estimar la gravedad de la enfermedad. Resultados. En el grupo con sepsis se incluyó a 38 pacientes y en el de referencia, a 40 niños sanos. Las concentraciones plasmáticas de tiol en los pacientes con sepsis fueron significativamente inferiores que las del grupo de referencia (p < 0,001). Conclusión. La homeostasis de tiol/disulfuro fue anormal en los niños con sepsis en la unidad de cuidados intensivos pediátricos.

The aim of this study is to evaluate a novel oxidative stress marker (thiol-disulphide homeostasis) in paediatric sepsis and to determine their effects on the prognosis of sepsis. Patients diagnosed with sepsis (n= 38) and healthy controls (n= 40) were incorporated in the study. Total thiol, native thiol, disulphide, disulphide/total thiol, disulphide/native thiol, and native thiol /total thiol levels were measured in the sepsis and control groups. Additionally, the parameters were compared between survivors and non-survivors in the sepsis group. The levels of hemoglobin, white blood cell, platelet, lactate, and C-reactive protein were measured in patients with sepsis at diagnosis. The paediatric risk of mortality and paediatric logistic organ dysfunction scores of the patients were used to estimate the disease severity. The plasma thiol levels of the patients with sepsis were significantly lower than the control group (p < 0.001). This study showed that thiol/disulphide homeostasis is abnormal in children with sepsis in Paediatric Intensive Care Unit.
Descritores: Compostos de Sulfidrila
Sepse
Estresse Oxidativo
Dissulfetos
Homeostase
Limites: Seres Humanos
Recém-Nascido
Lactente
Pré-Escolar
Criança
Adolescente
Tipo de Publ: Relatório Técnico
Responsável: AR94.1 - Centro de Información Pediatrica


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Id: biblio-837687
Autor: Ozturk, Barcin; Kurtoglu, Tunay; Durmaz, Selim; Kozaci, Leyla Didem; Abacigil, Filiz; Ertugrul, Bulent; Erel, Ozcan.
Título: The effects of ozone on bacterial growth and thiol-disulphide homeostasis in vascular graft infection caused by MRSA in rats
Fonte: Acta cir. bras;32(3):219-228, Mar. 2017. tab, graf.
Idioma: en.
Projeto: Adnan Menderes University.
Resumo: Abstract: Purpose: To investigate the microbiological, inflammatory and oxidant effects of adjuvant ozone administration in experimental rat vascular graft infection model which has not been previously investigated. Methods: Forty adult Wistar rats were divided into Sham, Control, Vancomycin, Ozone, Vancomycin+Ozone groups. Grafts were inoculated with Methicillin-resistant Staphylococcus aureus (MRSA) strain and implanted subcutaneously. Rats were treated intraperitoneally with ozone and /or intramuscularly with vancomycin for 10 days. Grafts were evaluated by quantitative bacterial cultures. Blood samples were harvested for determination of thiol-disulphide and cytokine profiles. Results: There was no significant difference in bacterial counts between Control and Ozone Groups. In the Ozone Group median colony count was significantly higher than the Vancomycin and Vancomycin+Ozone Groups. Total thiol and disulphide levels increased and disulphide/native thiol and disulphide/total thiol ratios decreased in Ozone Group significantly. Albumin levels decreased significantly in Vancomycin and Vancomycin+Ozone Groups compared to the Sham Group. IL-1 and TNF-alpha levels significantly increased in infected rats. Decreased levels of VEGF due to infection reversed by ozone therapy in control and vancomycin groups. Conclusions: We didn't observe any benefit of the agent on MRSA elimination in our model. Likewise, effects of ozone on thiol-disulphide homeostasis and inflammatory cytokines were contradictory.
Descritores: Oxidantes Fotoquímicos/farmacologia
Ozônio/farmacologia
Infecções Estafilocócicas/tratamento farmacológico
Dissulfetos/sangue
Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos
Enxerto Vascular
-Valores de Referência
Fatores de Tempo
Doenças Vasculares/microbiologia
Albumina Sérica/análise
Vancomicina/farmacologia
Contagem de Colônia Microbiana
Distribuição Aleatória
Reprodutibilidade dos Testes
Citocinas/sangue
Resultado do Tratamento
Ratos Wistar
Transplantes/microbiologia
Staphylococcus aureus Resistente à Meticilina/crescimento & desenvolvimento
Homeostase/efeitos dos fármacos
Antibacterianos/farmacologia
Limites: Animais
Masculino
Tipo de Publ: Estudos de Validação
Responsável: BR1.1 - BIREME


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Id: biblio-1008980
Autor: Tang, Feng; Chen, Daiwen; Yu, Bing; Luo, Yuheng; Zheng, Ping; Mao, Xiangbing; Yu, Jie; He, Jun.
Título: Improving the thermostability of Trichoderma reesei xylanase 2 by introducing disulfide bonds
Fonte: Electron. j. biotechnol;26:52-59, Mar. 2017. ilus, tab, graf.
Idioma: en.
Projeto: Fok Ying Tung Education Foundation; . "Prominent Young Scientist Fund" of Sichuan Province.
Resumo: Background: Xylanases are considered one of the most important enzymes in many industries. However, their low thermostability hampers their applications in feed pelleting, pulp bleaching, and so on. The main aim of this work was to improve the thermostability of Trichoderma ressei xylanase 2 (Xyn2) by introducing disulfide bonds between the N-terminal and α-helix and the ß-sheet core. Results: In this work, two disulfide bonds were separately introduced in the Xyn2 to connect the N-terminal and α-helix to the ß-sheet core of Xyn2. The two disulfide bonds were introduced by site-directed mutagenesis of the corresponding residues. The half-life of the mutants Xyn2C14­52 (disulfide bond between ß-sheets B2 and B3) and Xyn2C59­149 (disulfide bond between ß-sheets A5 and A6) at 60°C was improved by approximately 2.5- and 1.8-fold compared to that of the wild type Xyn2. In addition, the enzyme's resistance to alkali and acid was enhanced. Conclusion: Our results indicated that the connection of the N-terminal and α-helix to the ß-sheet core is due to the stable structure of the entire protein.
Descritores: Trichoderma/enzimologia
Xilosidases/metabolismo
Dissulfetos/metabolismo
-Espectrometria de Massas
Temperatura Ambiente
Trichoderma/genética
Trichoderma/metabolismo
Xilanos/metabolismo
Xilosidases/genética
Estabilidade Enzimática
Cinética
Mutagênese Sítio-Dirigida
Concentração de Íons de Hidrogênio
Mutação
Responsável: CL1.1 - Biblioteca Central


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Id: lil-747142
Autor: Bothe, Carolina; Fernandez, Alejandro; Garcia, Jacinto; Lopez, Montserrat; León, Xavier; Quer, Miquel; Lop, Joan.
Título: Parotid Incidentaloma Identified by Positron Emission/Computed Tomography: When to Consider Diagnoses Other than Warthin Tumor
Fonte: Int. arch. otorhinolaryngol. (Impr.);19(2):112-115, Apr-Jun/2015. tab.
Idioma: en.
Resumo: Introduction Parotid gland incidentalomas (PGIs) are unexpected hypermetabolic foci in the parotid region that can be found when scanning with whole-body positron emission/computed tomography (PET/CT). These deposits are most commonly due to benign lesions such as Warthin tumor. Objective The aim of this study was to determine the prevalence of PGIs identified in PET/CT scans and to assess the role of smoking in their etiology. Methods We retrospectively reviewed all PET/CT scans performed at our center in search of PGIs and identified smoking status and standardized uptake value (SUVmax) in each case. We also analyzed the database of parotidectomies performed in our department in the previous 10 years and focused on the pathologic diagnosis and the presence or absence of smoking in each case. Results Sixteen cases of PGIs were found in 4,250 PET/CT scans, accounting for 0.4% . The average SUVmax was 6.5 (range 2.8 to 16). Cytology was performed in five patients; it was benign in four cases and inconclusive in one case. Thirteen patients had a history of smoking. Of the parotidectomies performed in our center with a diagnosis of Warthin tumor, we identified a history of smoking in 93.8% of those patients. Conclusions The prevalence of PGIs on PET/CT was similar to that reported by other authors. Warthin tumor is frequently diagnosed among PGIs on PET/CT, and it has a strong relationship with smoking. We suggest that a diagnosis other than Warthin tumor should be considered for PGIs in nonsmokers. .
Descritores: Proteínas ADAM/metabolismo
Proteólise
Fator de von Willebrand/química
Fator de von Willebrand/metabolismo
-Sítios de Ligação
Cálcio/metabolismo
Dissulfetos/química
Dissulfetos/metabolismo
HEKABORTION, INCOMPLETEABATTOIRS CELLS
Ligações de Hidrogênio
Modelos Moleculares
Mutagênese Sítio-Dirigida
Ligação Proteica
Estabilidade Proteica
Estrutura Terciária de Proteína
Isoformas de Proteínas/química
Isoformas de Proteínas/metabolismo
Fator de von Willebrand/genética
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


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Id: lil-736436
Autor: Andrade, Dilma Maria de; Barbosa-Branco, Anadergh.
Título: Sinovite e tenossinovite no Brasil: uma análise dos benefícios auxílio-doença / Synovitis and tenosynovitis in Brazil: analysis of sickness benefit claims
Fonte: Rev. bras. epidemiol;18(1):194-207, Jan-Mar/2015. tab.
Idioma: pt.
Resumo: OBJETIVO: Analisar os fatores pessoais associados à prevalência e duração dos benefícios auxílio-doença decorrentes de sinovite e tenossinovite (CID10 M65). MÉTODO: Estudo transversal referente aos benefícios auxílio-doença decorrentes de sinovite e tenossinovite concedidos pelo Instituto Nacional de Seguro Social aos empregados no Brasil em 2008. Dados sobre o ramo de atividade econômica (Classificação Nacional de Atividades Econômicas - CNAE divisão, classe), sexo, idade, espécie e duração dos benefícios foram coletados do Sistema Único de Benefícios. A população corresponde à média mensal dos vínculos empregatícios declarados ao Cadastro Nacional de Informações Sociais. RESULTADOS: Em 2008 foram concedidos 35.601 benefícios auxílio-doença decorrentes de sinovite e tenossinovite, com prevalência de 10,9/10.000 vínculos empregatícios. No conjunto dos benefícios auxílio-doença houve maior razão de prevalência (RP) acidentária (RP 1,2), sendo esta maior em mulheres (RP 3,3), e em trabalhadores com idade acima de 39 anos (RP 1,4). As CNAE 37-Esgoto (55,4) e 60-Atividade de rádio e TV (47,1) apresentaram as maiores prevalências, no entanto, 64-Atividade de serviços financeiros e 6422-Bancos múltiplos caracterizaram mais acidentes de trabalho (RP 3,2 e 3,8, respectivamente) e maior duração (70 e 73 dias, respectivamente). A maior duração de benefício ocorreu entre trabalhadores com idade superior a 39 anos. Tanto a CNAE-divisão 60-Atividade de rádio e TV, quanto a CNAE-classe 6010-Atividade de rádio apresentaram elevadas razões de feminilidade (RP 8,1 e 10,8, respectivamente). CONCLUSÃO: A incapacidade para o trabalho por sinovite e tenossinovite apresenta associação tanto da prevalência quanto da duração com o ramo de atividade, sexo, idade e espécie de benefício (previdenciário/acidentário). .

OBJECTIVE: To analyse the personal and occupational factors associated with the prevalence and duration of sickness benefit claims due to synovitis and tenosynovitis (CID10 M65). METHODS: Cross-sectional study regarding sickness benefit claims due to synovitis and tenosynovitis granted to employees by National Institute of Social Security in Brazil in 2008. Data on economic activity (Economic Activities National Classification - CNAE division, class), sex, age, type and duration of benefits were collected from the Unified Benefit System. The study's population consists of the average monthly employment contracts declared to the National Register of Social Information. RESULTS: In 2008, 35,601 employees were granted sickness benefits due to synovitis and tenosynovitis, with a prevalence of 10.9/10,000 employments. Sickness benefits showed higher prevalence rates (PR) for work-related claims (PR 1,2), mostly made by females (PR 3.3) and by workers older than 39 years (PR 1,4). The CNAE 37-Sewage (55.4) and 60-Broadcasting Activity (47.1) had the highest overall prevalence. However, the 64-Financial service activities, except insurance and pension funding and 6422-Multiple banks with commercial service had the highest rates of work-related claims (RP 3.2 and 3.8, respectively), and the longer duration (70 and 73 days, respectively). Workers older than 39 years had the highest durations of work disability claims. Both the CNAE-division 60-Broadcasting Activity, and the CNAE-class 6010-Radio showed a high activity ratio of females (PR 8.1 and 10.8, respectively). CONCLUSION: The work disability due to synovitis and tenosynovitis presents prevalence and duration associated with economic activity, sex, age and kind of benefit (non work-related and work-related claims). .
Descritores: Globinas/química
Peróxido de Hidrogênio/química
Proteínas do Tecido Nervoso/química
Nitritos/química
-Sequência de Aminoácidos
Sítios de Ligação
Catálise
Cisteína/química
Cisteína/metabolismo
Dissulfetos/química
Dissulfetos/metabolismo
Globinas/metabolismo
Peróxido de Hidrogênio/metabolismo
Cinética
Modelos Moleculares
Dados de Sequência Molecular
Mioglobina/química
Mioglobina/metabolismo
Proteínas do Tecido Nervoso/metabolismo
Nitritos/metabolismo
Oxirredução
Conformação Proteica
Fenol/química
Fenóis/química
Fenilacetatos/química
Espectrometria de Massas em Tandem
Limites: Seres Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Texto completo SciELO Venezuela
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Id: lil-659221
Autor: Vivas, Julio; Alvarado, Primavera; Visbal, Gonzalo; Álvarez-Aular, Álvaro; Ruiz, Egle; Ledezma, Eliades.
Título: Sinergismo in vitro entre hidrazonas, ajoeno y posaconazol sobre aislados de Cryptococcus spp / In vitro synergisms among hydrazones, ajoeno and posaconazole against Cryptococcus spp
Fonte: Invest. clín;52(4):312-322, dic. 2011. ilus, tab.
Idioma: es.
Resumo: El objetivo de este trabajo fue estudiar la susceptibilidad in vitro de aislados de Cryptocococus spp con una nueva clase de antifúngicos, hidrazonas esteroidales y comparar su actividad antifúngica en combinación con ajoeno y posaconazol contra aislados de Cryptococcus spp. Se utilizaron tres aislados del género Cryptococcus 42794, 4050 y 44192 y se evaluaron su sensibilidad y efectos sinérgicos con las hidrazonas esteroidales, ajoeno y posaconazol, según el documento M27-A2 del CLSI. Se incluyeron las cepas Candida albicans (ATCC 90028) y Candida parapsilosis (ATCC 22019) como controles. Se observó con las hidrazonas (H1, H2, H3, H4) un efecto plateau a partir de 10 µM (CMI). Sin embargo, con la H4 se obtuvo bajo porcentaje de inhibición del crecimiento. Con el ajoeno, se obtuvieron valores de CMI de 25 y 50 µM. El posaconazol mostró altos valores de inhibición y un valor de CMI de 6 µM para 42794 y 44192 y un CMI de 20 µM para el aislado 4050. Se obtuvieron efectos sinérgicos al combinar posaconazol con ajoeno, ajoeno con hidrazona 3 y posaconazol con hidrazona 3. Los valores de concentración inhibitoria fraccional fueron de 0,24; 0,16 y 0,09 respectivamente, indicando un marcado efecto sinérgico. Se obtuvieron efectos sinérgicos importantes entre el posaconazol con ajoeno, ajoeno con hidrazona 3 y posaconazol con hidrazona 3, lo cual sería muy útil para futuros estudios clínicos.

The aim of this study was to assess the in vitro susceptibility to novel antifungal compounds, the steroidal hydrazones, and to compare their antifungal activity and synergistic effects with other compounds, such as ajoeno and posaconazole on Cryptocococus spp isolates. Three Cryptococcus strains were used for this study (42794, 4050 and 44192) and their antifungal sensitivity and synergistic effects with ajoeno and posaconazole were evaluated according to the CLSI protocol number M27-A2. Candida albicans (ATCC 90028) and Candida parapsilosis (ATCC 22019) were used as controls. A plateau effect with hydrazones (H1, H2, H3, H4) was observed after 10 µM (CMI). However, with H4 only a mild inhibition on the growth was obtained. Combining hydrazone and ajoeno, CMI values between 25 and 50 µM were obtained. The highest inhibitions values were obtained with posaconazole and a CMI value of 6 µM for the strains 42794 and 44192, and a CMI value of 20 µM for the strain 4050. Synergy was observed combining posaconazole with ajoeno, ajoeno with hydrazone 3 and posaconazole with hydrazone 3. Fractional inhibitory concentrations were 0.24, 0.16 and 0.09 respectively, which might indicate a synergistic effect. Important synergistic effects were obtained with posaconazole and ajoeno, ajoeno and hydrazone 3 and posaconazole with hydrazone 3, which would be very useful for clinical trials in the future.
Descritores: Antifúngicos/farmacologia
Cryptococcus gattii/efeitos dos fármacos
Cryptococcus neoformans/efeitos dos fármacos
Dissulfetos/farmacologia
Hidrazonas/farmacologia
Técnicas In Vitro
Triazóis/farmacologia
-Candida/efeitos dos fármacos
Relação Dose-Resposta a Droga
Avaliação Pré-Clínica de Medicamentos
Farmacorresistência Fúngica
Sinergismo Farmacológico
Testes de Sensibilidade Microbiana
Tipo de Publ: Estudo Comparativo
Responsável: VE1.1 - Biblioteca Humberto Garcia Arocha


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Id: lil-639942
Autor: Ayala Corredor, Catalina; Solís Castillo, Carolina.
Título: Otras posibles aplicaciones clínicas de fármacos con efecto 5HT2A y 3 en psiquiatría de enlace: reporte de casos / Other Possible Clinical Applications of Drugs with 5HT2A effect in Liaison Psychiatry: Cases Report
Fonte: Rev. colomb. psiquiatr;41(1):217-228, ene.-abr. 2012. ilus, graf, tab.
Idioma: es.
Resumo: Introducción: En psiquiatría de enlace se logra obtener una visión integral del tratamiento y de las necesidades de cada paciente prestando especial atención a las interacciones medicamentosas y a las contraindicaciones. Algunos casos particulares motivaron la descripción, reporte y revisión bibliográfica acerca de otras posibles aplicaciones de fármacos antagonistas de los recetores 5HT2A y 3, particularmente mirtazapina y olanzapina, en síndrome de hiperalgesia, tinitus y leucoencefalopatía multifocal progresiva por virus JC. Método: reporte de casos. Resultados y Conclusiones: Se describen los casos de tres pacientes en los cuales fue necesario usar mirtazapina y olanzapina no solo para el control de los síntomas psiquiátricos (afectivos, comportamentales y trastorno del sueño), sino también como coadyuvantes en las patologías de base de cada paciente. El uso de cualquier medicamento en psiquiatría de enlace debe tener en cuenta el contexto del paciente, la comorbilidad, las contraindicaciones y las interacciones farmacológicas para garantizar un desenlace positivo, además de promover el trabajo multidisciplinario entre especialistas.

Introduction: In liaison psychiatry it is possible to get an integral view of patient's treatment and needs, paying special attention to pharmacological interactions and contraindications. Some particular cases motivated the description, report and review about other possible applications of 5HT2A and 5HT3 antagonist, particularly Mirtazapine and Olanzapine, in hyperalgesia syndrome, tinnitus and Progressive Multifocal Leukoencephalopathy by JC virus. Method: Cases report. Results: We describe 3 cases of patients in which Mirtazapine and Olanzapine were necessary not only to control psychiatric symptoms (affective / behavioral symptoms and insomnia) but to act as adjuvant therapy in axis III diseases. The use of any drug in psychiatry must take in to account the context of the patient, the presence of comorbidity, contraindications and pharmacological interactions so as to grant a positive outcome also promoting the multidisciplinary work between specialists.
Descritores: Proteínas Adaptadoras de Transdução de Sinal/metabolismo
Núcleo Celular/metabolismo
Cisteína/metabolismo
Neurônios/metabolismo
Tiorredoxinas/metabolismo
Fatores de Transcrição/metabolismo
-Motivos de Aminoácidos
Proteínas Adaptadoras de Transdução de Sinal/genética
Linhagem Celular Tumoral
Cisteína/química
Citoplasma/metabolismo
Dissulfetos/química
Perfilação da Expressão Gênica
Regulação da Expressão Gênica
Anotação de Sequência Molecular
Dados de Sequência Molecular
Mutação
Neurônios/citologia
Oxirredução
Mapeamento de Interação de Proteínas
Transdução de Sinais
Transcrição Genética
Tiorredoxinas/genética
Fatores de Transcrição/genética
Limites: Seres Humanos
Tipo de Publ: Research Support, N.I.H., Extramural
Responsável: CO332 - Facultad de Medicina


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Texto completo SciELO Chile
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Id: lil-582857
Autor: Votto, Ana P. S; Domingues, Beatriz S; de Souza, Michele M; da Silva Júnior, Flavio M. R; Caldas, Sergiane S; Filgueira, Daza M. V. B; Clementin, Rosilene M; Primel, Ednei G; Vallochi, Adriana L; Furlong, Eliana B; Trindade, Gilma S.
Título: Toxicity mechanisms of onion (Allium cepa) extracts and compounds in multidrug resistant erythroleukemic cell line
Fonte: Biol. Res;43(4):429-437, 2010. ilus.
Idioma: en.
Resumo: Onion (Allium cepa) is being studied as a potential anticancer agent, but little is known regarding its effect in multidrug resistance (MDR) cells. In this work, the cytotoxicity of crude onion extract (OE) and fractioned extract (aqueous, methanolic and ethyl acetate), as well as some onion compounds (quercetin and propyl disulfide) were evaluated in Lucena MDR human erythroleukemic and its K562 parental cell line. The capacity of OE to induce apoptosis and/or necrosis in these cells, the possible participation of oxidative stress and DNA damage were also assessed. Similar sensitivities were obtained for both tumoral cells, however only OE caused significant effects in the cells. In K562 cells, a significant increase of apoptosis was verified while the Lucena cells experienced a significant increase of necrosis. An antioxidant capacity was verified for OE discarding oxidative damage. However, OE provoked similar significant DNA damage in both cell lines. Thus, the OE capacity to overcome the MDR phenotype suggests anti-MDR action of OE.
Descritores: Antineoplásicos Fitogênicos/farmacologia
Resistência a Medicamentos Antineoplásicos/efeitos dos fármacos
Cebolas/química
Extratos Vegetais/farmacologia
-Apoptose
Dano ao DNA
Dissulfetos/análise
Dissulfetos/farmacologia
/efeitos dos fármacos
KAMDINOCILLIN PIVOXIL CELLS/efeitos dos fármacos
LETHAL DOSE ACADEMIES AND INSTITUTES
Necrose
Fenótipo
Quercetina/análise
Quercetina/farmacologia
Fatores de Tempo
Limites: Seres Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-575310
Autor: Acosta L., Orlando; Calderón, Martha; Moreno, Liz; Guerrero F., Carlos A.
Título: Un modelo del mecanismo de entrada de los rotavirus a la célula hospedera / A model for the mechanism of entry of rotaviruses into the host cell
Fonte: Rev. Fac. Med. (Bogotá);57(2):124-148, abr.-jun. 2009.
Idioma: es.
Resumo: Antecedentes. La infección rotaviral es causa principal de gastroenteritis aguda severa en niños menores de cinco años. La capa protéica externa de la partícula viral está implicada en las interacciones iniciales virus-superficie celular. El mecanismo rotaviral de unión y entrada a la célula parece ser un proceso de múltiples pasos donde las proteínas rotavirales VP4 y VP7 interaccionan con diferentes moléculas de la superficie celular. Objetivo. Proponer un mecanismo de entrada de rotavirus a la célula que incorpore la actividad de la proteína disulfuro isomerasa (PDI). Material y métodos. Utilizando bases de datos electrónicas, se realizó una búsqueda de literatura original y de revisión publicada entre 1990 y 2009 sobre moléculas de la superficie rotaviral o celular participantes en el proceso de entrada del virus. El análisis de los resultados enfatizó las bases moleculares y celulares de las interacciones temporo-espaciales de las proteínas virales y las moléculas de unión/receptoras de la célula. Resultados. Se encontró fundamentos moleculares y celulares para incorporar la actividad de PDI a un mecanismo coherente de vías secuenciales o alternativas previas a la penetración viral. Se propone un mecanismo en que interaccionan las proteínas virales VP4, VP6 y VP7 con las moléculas de la superficie celular ácido siálico, integrinas, Hsc70 y PDI en un proceso endocítico caveola/“raft”-dependiente, caveolina/clatrina-independiente, dinamina-dependiente y sensible a depleción de colesterol. Conclusión. Se amplía el concepto de múltiples pasos en el proceso de entrada de rotavirus, donde la participación de PDI podría ser un blanco potencial de la acción de inhibidores de grupos tiol/disulfuro...
Descritores: Proteínas de Choque Térmico
Integrinas
Rotavirus
-Dissulfetos
Isomerases
Limites: Seres Humanos
Tipo de Publ: Estudos de Validação
Responsável: CO136.2 - Biblioteca


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Id: lil-539713
Autor: Ling, H; Wen, L; Ji, X. X; Tang, Y. L; He, J; Tan, H; Xia, H; Zhou, J. G; Su, Q.
Título: Growth inhibitory effect and Chk1-dependent signaling involved in G2/M arrest on human gastric cancer cells induced by diallyl disulfide
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;43(3):271-278, Mar. 2010. ilus, tab, graf.
Idioma: en.
Resumo: Diallyl disulfide (DADS) inhibits growth and induces cell cycle G2/M arrest in human gastric cancer MGC803 cells. In this study, 15 mg/L DADS exerted similar effects on growth and cell cycle arrest in human gastric cancer BGC823 cells. Due to the importance of cell cycle redistribution in DADS-mediated anti-carcinogenic effects, we investigated the role of checkpoint kinases (Chk1 and Chk2) during DADS-induced cell cycle arrest. We hypothesized that DADS could mediate G2/M phase arrest through either Chk1 or Chk2 signal transduction pathways. We demonstrated that DADS induced the accumulation of phosphorylated Chk1, but not of Chk2, and that DADS down-regulated Cdc25C and cyclin B1. The expression of mRNA and total protein for Chkl and Chk2 was unchanged. Chk1 is specifically phosphorylated by ATR (ATM-RAD3-related gene). Western blot analysis showed that phospho-ATR was activated by DADS. Taken together, these data suggest that cell cycle G2/M arrest, which was associated with accumulation of the phosphorylated forms of Chk1, but not of Chk2, was involved in the growth inhibition induced by DADS in the human gastric cancer cell line BGC823. Furthermore, the DADS-induced G2/M checkpoint response is mediated by Chk1 signaling through ATR/Chk1/Cdc25C/cyclin B1, and is independent of Chk2.
Descritores: Compostos Alílicos/farmacologia
Antineoplásicos/farmacologia
Dissulfetos/farmacologia
/efeitos dos fármacos
GTEMEFOS PHASE/efeitos dos fármacos
Inibidores do Crescimento/farmacologia
Proteínas Quinases/efeitos dos fármacos
Neoplasias Gástricas/enzimologia
-Linhagem Celular Tumoral
Divisão Celular/efeitos dos fármacos
Proteínas Quinases/metabolismo
Transdução de Sinais/efeitos dos fármacos
Neoplasias Gástricas/patologia
Limites: Seres Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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